Objective To discuss the relationships between expression levels of CD147 and vascular endothelial growth factor C (VEGF-C) in lung squamous cell carcinoma and adenocarcinoma and the clinicopathological features.Methods The expression of CD147 and VEGF-C in 57 lung cancer tissues was detected by immunohistochemical method.Results The positive expression rates of CD147 and VEGF-C in 57 lung cancer tissues were 63.16 ％ (36/57),66.67 ％ (38/57) respectively.The expression of CD147 and VEGF-C in lung squamous cell carcinoma and adenocarcinoma had no statistical difference (both P ＞ 0.05).The expression of CD147 and VEGF-C in lung squarnous cell carcinoma and adenocarcinoma had relationship with lymph node metastasis and tumor TNM stage (all P ＜ 0.05),however they had not correlation with the patient' s age,sex,smoking and tumor cell differentiation extent irrelevant (all P ＞ 0.05).The expression of CD147 was related positively with VEGF-C (P ＜ 0.01).Conclusions CD147 and VEGF-C play important roles in the progression and metastasis of lung squamous cell carcinoma and adenocarcinoma,which may have a synergistic effect.The joint detection of CD147 and VEGF-C can be used as a useful prognostic indicator in patients with lung cancer.

Objective To investigate the correlation between the expression of CD147 and MUC5ac in peripheral small cell lung cancer and the computed tomography (CT) signs.Methods Forty two cases of peripheral non-small cell lung cancer (NSCLC) confirmed by operation and pathology were examined by CT.The positive expression of CD147 and MUC5ac was detected by immunohistochemical staining and the relationship between the expression of CD147 and MUC5ac was analyzed.Results CD147 and MUC5ac were closely related to the lymphatic metastasis of peripheral non-small cell lung cancer (P ＜ 0.05).The positive expression of CD147 is significantly related to tumor size,deep lobulation,spinous process and lymphadenopathy (P ＜ 0.05).MUC5ac is significantly related to tumor size,deep lobulation,spiculation,vascular convergence sign,extrapleural fat Line disappearance,and mediastinal lymphadenopathy (P ＜0.05).Conclusions These results indicate that CD147 and MUC5ac play an important role in the invasion and metastasis of peripheral lung cancer.

Objective To investigate the application of dual volume reconstruction translucent imaging in performing stent- assisted coil embolization for intracranial aneurysm embolization treatment. Methods During the period from Nov. 2011 to Sep. 2012, a total of 30 patients with intracranial aneurysm were admitted to authors’ hospital. Stent-assisted coil embolization was carried out in all patients. The number of all the stent point-marks visualized on routine 2D-DSA, on rotational angiography (3D-RA) and on dual volume reconstruction translucent images were determined, and the results were compared between each other of the three imaging methods. Results A total of 34 stents (206 stent point-marks in total) were implanted in the 30 patients. Of the 206 stent point-marks, 2D-DSA, 3D-RA and dual volume reconstruction translucent image could clearly display 146 (70.8%), 123 (59.7%) and 190 (92.2%), respectively. Statistically significant difference in the displaying rate of the stent point-marks existed between each other among the three imaging methods (P < 0.05). Conclusion Dual volume reconstruction translucent imaging can distinctly display the location of the stent marks, which is of great value in guiding the performance of intracranial stent implantation surgery.

Objective:To construct an adenoviral vect or carrying human tissue inhibitor of metalloproteinase-2(TIMP-2)gene in order to mediate the expression of TIMP-2 gene in vascular smooth muscle cells(SMC)i n vitro. Methods:A recombinant adenovirus(AdhTIMP-2)containging human TIM P-2 cDNA fragment was generated by homologous recombination in BJ5183 bacteria. Recombinant plasmids were screened by alteration of antibiotics.The adenovirus v ector was then packaged and amplified in 293 cells.The SMC of rat aortic were is olated and cultured in vitro and been infected with AdhTIMP-2.The expression of TIMP-2 was detected by the techniques of Western blot and RT-PCR. Results:The recombinant adenoviral vector carrying human TI MP-2 was constructed. The titer was 4?1011efu/ml after purification.The AdhTIMP-2 could infect the cultured VSMC efficiently(MOI=100,infection ra te=94%).The expression of TIMP-2 gene in those infected cells was detected by R T-PCR.After the cells were infected with AdhTIMP-2 24hours,TIMP-2 protein cou ld be detected in the conditioned medium by Western blot. Conclusion:The recombinant adenoviral vector carrying human TIMP -2 is successfully constructed and AdhTIMP-2 can efficently mediate the expres sion of TIMP-2 gene in cultured VSMC,paving the way for further application in vascular disease gene therapy.

Objective To investigate the effect of TIMP-2 gene that was transfected by adenovirus on extracellular matrix of abdominal aortic through assessing the changes of morphology and histopathology of the rat models with abdominal aortic aneurysm. Methods The rat models with abdominal aortic aneurysm were constructed by intraluminally perfusing porcine pancreatic elastase. Twenty-four SD rats with aneurysm were then randomly divided into 3 groups: AdTIMP-2 group (perfused locally with solution of TIMP-2 gene transfected by adenovirus vector to abdominal aorta), AdCMV group (transfected by non-viral vector), and PBS group. After 14 days, the concentrations of elastin and collagen that were collected from the samples of aortic wall were measured by image analysis system and the fixed aortic tissues were examined by light microscopy and some other specific staining methods. Results None of abdominal aortic aneurysm developed in TIMP-2 gene transfected group, with significantly higher rates of developed aneurysm in the other groups (P

Objective To investigate the effect ofmicroRNA-101(miR-101) overexpression on proliferation and metastasis of human hepatoma HepG2 cells and the molecular mechanism.Methods HepG2 cells were divided into blank group,negative control group and miR-101 transfection group.HepG2 cell line stably overexpressing miR-101 was established by lentiviral vector.The overexpression of miR-101 was detected by chemiluminescence method.The expression of vascular endothelial growth factor (VEGF) protein was detected by Western Blot.Scratch experiments was used to analyze the cell migration and the Transwell assay was used to detect cell proliferation.Results The expression of miR-101 in HepG2 cells was significantly increased after transfection with miR-101,and there was a direct targeting relationship between miR-101 and VEGF.Compared with the negative control group,the VEGF protein level in the miR-101 transfected group was significantly down-regulated,and the difference was statistically significant (P＜0.01).Moreover,the cell scratch healing ability and invasion ability were decreased in the miR-101 transfected group.Conclusions Overexpression of miR-101 can inhibit invasion and migration of human hepatoma HepG2 cells by targeting VEGF.

Objective To explore the correlation between lipoprotein-associated phospholipase A2 (Lp-PLA2) and perioperative myocardial injury (PMI),and to discuss the predictive value of Lp-PLA2 in patients with stable angina after percutaneous coronary intervention (PCI).Methods A total of 222 consecutive patients with stable angina,who were admitted to Yixing Municipal People's Hospital,Jiangsu Province,China to receive PCI during the period from June 2015 to March 2017,were enrolled in this study.The patients' baseline data as well as the distribution pattern of coronary lesions,were recorded.According to the paclitaxel-PCI and the surgical cooperative study (SYNTAX) score,the severity of target vascular lesions was assessed,which was classified into low score group (0 to 22 points),middle score group (23 to 32 points) and high score group (≥33 points).The preoperative blood lipid level and renal function,both preoperative and postoperative Troponin T (cTnT),high sensitive C reactive protein(hs-CRP),as well as the postoperative Lp-PLA2 were tested.Results After the procedure,the Lp-PLA2 levels in patients with normal cTnT value (n=155) and in patients with elevated cTnT value (n=67) were(122.21±43.80) ng/ml and (224.53±65.00) ng/ml respectively (P＜0.05).SYNTAX score analysis showed that low score group had 120 patients,middle score group had 78 patients and high score group had 24 patients,the Lp-PLA2 levels of the above three groups were (119.51±51.96) ng/ml,(178.67±61.49) ng/ml and (233.16±61.32) ng/ml respectively,the differences were statistically significant between each other among the three groups (P＜0.05).Pearson correlation analysis indicated that a parallel correlation existed between Lp-PLA2 levels and postoperative cTnT values (R=0.492,P＜0.05).Logistic regression analysis revealed that Lp-PLA2 was the independent risk factor for elevated cTnT value during the perioperative period of PCI (OR=7.377,95％CI=3.368-16.156,P＜0.05).The area under ROC curve of Lp-PLA2 was 0.896 (95％CI=0.874-0.945,P＜0.001),the best cut-off point was 179 ng/ml,and the sensitivity and specificity for the diagnosis of PMI were 92.2％ and 66.7％,respectively.Conclusion Lp-PLA2 levels are closely correlated with the increased cTnT values after PCI,and the preoperative high level of Lp-PLA2 is the independent risk factor for PMI after PCI.

Objective To explore the measurement accuracy of right coronary artery during coronary angiography (CAG)at 45Gdegree left oblique position.Methods The images of right coronary artery angiographic view of 45Gdegree left oblique position were divided into nine average areas.The images with marker segment of guiding wire located in the central area (n＝45)or subcentral area (n＝45)of CAG cases were collected retrospectively and randomly.The marker segment was measured by catheter calibration method,and the measure values were compared with the actual length (30 mm).Results A N OVA analysis suggested statistical differences among the three groups (F＝4.59,P＜0.05).By paired comparison,no significant differences were found in measured values between central areas (3 1.1 9± 4.12)mm or subcentral areas (29.55±2.75)mm and the actual length (P＞0.05).Significant differences were found in measured values between central areas and subcentral areas (P＜0.05).The measuring error of subcentral areas (－1.5%)was less than that of central areas (3.9%).Conclusion During CAG at 45Gdegree left oblique position,the values of subcentral area were more accurate than those of central area and the subcentral area was thus regarded as image area with less measurement error in interventional surgery.

OBJECTIVETo quantitatively analyze the temporal and spatial pattern of RhoA expression in injured spinal cord of adult mice.

METHODSA spinal cord transection model was established in adult mice. At 1, 3, 7, 14, 28, 56 and 112 days after the surgery, the spinal cords were dissected and cryosectioned for RhoA/NF200, RhoA/GFAP, RhoA/CNPase or RhoA/IBA1 double fluorescent immunohistochemistry to visualize RhoA expressions in the neurons, astrocytes, oligodendrocytes and microglia. The percentages as well as the immunostaining intensities of RhoA-positive cells in the parenchymal cells were quantitatively analyzed.

RESULTSRhoA was weakly expressed in a few neurons and oligodendrocytes in normal spinal cord. After spinal cord injury, the percentage of RhoA-positive cells and RhoA expression intensity in the spinal cord increased and peaked at 7 days post injury (dpi) in neurons, oligodendrocytes and astrocytes, followed by a gradual decrease till reaching a low level at 112 dpi. In the microglia, both the RhoA-positive cells and RhoA expression intensity reached the maximum at 14 dpi and maintained a high level till 112 dpi.

CONCLUSIONTraumatic spinal cord injury can upregulate RhoA expression in the neurons as well as all the glial cells in the spinal cord. RhoA expression patterns vary with post-injury time, location and among different parenchymal cells in the injured spinal cord.

Animals ; Astrocytes ; metabolism ; Female ; Mice ; Mice, Inbred Strains ; Microglia ; metabolism ; Neuroglia ; metabolism ; Neurons ; metabolism ; Spinal Cord ; metabolism ; Spinal Cord Injuries ; metabolism ; rho GTP-Binding Proteins ; metabolism

Objective To explore the differences of aortic diameter measured by catheter calibration method and centimeter sizing catheter calibration method in endovascular graft exclusion for Stanford type B aortic dissection.Methods A total of 30 patients with Stanford B type aortic dissections treated with endovascular graft exclusion were measured with the two calibration methods to measure aortic diameters at left subclavicular artery position.The measurement parameters were compared with CTA measurement results.Results Statistical differences of measurement parameters were found among catheter calibration,centimeter sizing catheter calibration and CTA (F=3.15,P＜0.05),and paired comparison showed statistical differences between catheter calibration method and CTA result (P＜0.05),and between the results of catheter calibration method and centimeter sizing catheter calibration method (P＜0.05),while no statistical difference was found between centimeter sizing catheter calibration method and CTA result (P＞0.05).Taking CTA as golden standard method,Bland-Altman analysis indicated that the centimeter sizing catheter calibration method exerted better consistency with CTA.Conclusion The measurement value of centimeter sizing catheter calibration method is identical to the CTA measurement result,and superior to catheter calibration method for precise stent selection in Stanford type B aortic dissection.