Objective:To introduce a method and theory about how implementation vein image projection epidermis and conduct the clinical testing.Methods: Base on existing electronic components construct circuit. The construct circuit was made of industrial personal computer, mini-projector, camera and so on. The image is captured by camera transfer to industrial personal computer. Choosing suitable image process algorithms modify image. Processed image that storage in industrial personal computer transfer to mini-projector, vascular vein lines is produced from mini-projector.Results: Through this way that vascular vein projector method get image basic agreement vascular trend, meet the clinical needs.Conclusion: Vein image projection methods meet clinical need can be used to study other field.

In order to solve some limitations and difficulties in guiding the practical work of determination and classification of the areas of high water iodine and the endemic areas of iodine excess goiter (GB/T 19380-2003),we carried out the revision on the original standard from 5 aspects such as the name,normative reference file,the definition and the technical indicators of classification of high iodine areas and the endemic areas of iodine excess goiter,such as sampling method in appendix and so on,and worked out the standard of definition and demarcation of water-borne iodine-excess areas and iodine-excess endemic areas.Enforcement of the new standard will bring important social benefits.

Objective To investigate the effect of propofol preconditioning on activities of ATPase on myocardial cell membrane in rats with myocardial ischemia-reperfusion injury. Methods Forty Sprague-Dawiey rats were subject to 30 min ischemia and 60 min reperfusion and randomly assigned to five groups, six rats per group: control(C) group; ischernia-reperfusion (IR) group; propofol preconditioning group(P, according to the dosage of propofol, P was assigned to three KM+-ATPase and Ca2+-Mg2+-ATPase of left ventricle myocardial tissue were measured by chemical colorirnetric method. The rnyocardium pathomorphism was observed with light microscopes. Results The activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in ischemia-reperfusion group were lower than control group and propofol preconditioning group(P<0. 05 or P<0.01). Conclusion Propofol preconditioning can produced cardioprotective effect against ischemia-reperfusion injury by recovering the ATPase activities of myocardial tissue.

Tumor microenvironment plays a crucial role in neoplastic evolution process as it may provide functional interactions between neoplastic and non-neoplastic cells involved in tumor invasion,metastasis and angiogenesis,which implies that it can be regarded as a novel target for the antitumor therapy of polysaccharides.This review focuses on the protumor variations existing in tumor microenvironment,such as growth factors,immunosuppressive factors,proteases,glycoproteins,etc.,which may offer possible mechanisms of polysaccharides on the regulation of their corresponding functions.

Objective To study the origin of HIV-1 strains from 2 mother-to-child cases. Method DNA fragments of HIV-1 gag gene were amplified by nested PCR from the 4 target persons, and the amplified region were sequenced. Results The sequence of the specified fragment from all persons were analyzed and the strains were belonged to subtype A/E, while the sequence between mother's and child's is highly homologous. Conclusion 2 children were infected by mother-to-child transmission.

Objective To study the value of P 300 in judging the true and false coma in patients with closed craniocerebral trauma. Methods The latency and amplitude of abnormal P 300 evoked by visual (image) stimulation were compared and analyzed between 237 cases with closed craniocerebral trauma.which were divided into coma,non coma,false coma and noclear group.Results There was very remarkable diffe rence in the latency and amplitude of P 300 between coma group and non coma group ( P 0 05). There was remarkable difference between the cases whose coma histories were unclear and non coma ( P

Objective To investigate changes of the visual P300 topography mapping in patients with closed craniocerebral trauma.Methods The visual P300 topography mapping was recorded from 103 patients with closed craniocerebral trauma and 66 normal subjects with a medicide-03E brain evoked potential instrument. Results The P300 latency in patient group was significantly prolonged as compared with the control group ( P

Objective To evaluate the clinical effect of immediate implant restoration in lower posterior teeth . Methods 31 XIVE implants were placed in lower posterior teeth of 31 patients.The temporary fixed resin crowns were fabricated immediately and final restorations were finished in 3~4 months.The control group used nonsub-merged implants and were restored with final crowns in 3~4 months.The tracking survey was taken from 12 to 24 months after final restoration .The patient satisfaction , papilla index score ( PIS ) and implant survival rate were eval-uated between the two groups .Results PIS of patients with immediate restoration reached (2.35 ±0.70) in aver-age compared(1.84 ±0.74) in control group.The patient satisfaction reached (91.5 ±7.5) with immediate resto-ration, compared with VAS score in average of(85.8 ±8.1) in control group.All implants in both groups achieved osseointegration and received 100% survival rate .Conclusion With proper technique in surgical and restoration procedure , immediate restoration after implant insertion in posterior madible has similar clinical success compared with delayed restoration .

Objective:To establish a method for the determination of chloramphenicol residue in Wuji Baifeng pills by liquid chro -matography tandem mass spectrometry with normal solid-phase extraction .Methods:A normal solid-phase extraction column was used for the sample pretreatment and enrichment , and high performance liquid chromatography tandem mass spectrometry was used to deter-mine chloramphenicol residue in Wuji Baifeng pills .The analysis was performed on an Agilent-ODS C18 (250 mm ×4.6 mm,5 μm) column .The mobile phase was composed of methanol and water with gradient elution at 40℃ (0-7 min, 30% methanol;7-15 min, 30%-80%methanol;15-25 min, 80%-30%methanol).The flow rate was 1.0 ml· min-1 , and the quantitation ion was m/z 152.1 (the negative ionization) under the mode of multiple reaction monitoring .Results:The limit of detection was 0.016 ng.The calibra-tion curves were linear within the range of 0.187-3.749 ng.The method recovery was 94.3% with RSD of 3.19%(n=9).Conclu-sion:The method is simple with accurate results .It is suitable for the determination of chloramphenicol residue .

Objective To synthesize 24-methylenecholestan-3?,5?,6?-triol,a compound same as a marine natural product,.Methods The target molecule was synthesized in 7 steps using hyodeoxycholic acid as the starting material,3?-(tetrahydropyran-2-yloxy)-cholest-5-ene-24-ketone as the key intermediate and isopropylation in Grignard reagents as the key step.Results The target molecule was synthesized in a total yield of 53% and confirmed by mass spectrometry(MS),proton magnetic resonance spectroscopy(1HNMR),carbon magnetic resonance spectroscopy,(13CNMR)and infrared spectroscopy(IR).Conclusion Our method to synthesize 24-methylene-cholestan-3?,5?,6?-triol is characterized by easily available and cheap starting material,short synthetic route,high yield and atom-economic.