1.Cytoskeletal vimentin protein expression in rats with exhaustive eccentric exercise injury
Chinese Journal of Tissue Engineering Research 2014;(18):2880-2885
BACKGROUND:Due to different experimental methods, the change of cytoskeleton proteins after centrifugal movement is stil controversial.
OBJECTIVE:To establish exhaustive eccentric exercise injury model in rats and to observe cytoskeletal vimentin protein expression at different time.
METHODS:A total of 48 male Sprague-Dawley rats were randomly and equal y divided into six groups:quiet control group, immediately after exercise group, and 12, 24, 48, 72 hours after exercise groups. In the exercise groups, the rats were subject treadmil exercise at the speed of 16 m/min in a-16° slope, for 100 minutes at the interval of 5 minutes. The quiet control group maintained unchanged, without exercise. The cytoskeletal vimentin was detected with immunohistochemical staining using anti-vimentin antibody, and vimentin expression changes were observed at different time after exhaustive eccentric exercise through the changes of target area percentage.
RESULTS AND CONCLUSION:The results of target area percentage showed that, there was no significant difference between quiet control group and immediately after exercise group (P>0.05). Compared with the immediately after exercise group, the target area percentage was slightly increased in 12 hours after exercise group, without significant difference (P>0.05). Compared with the 12 hours after exercise group, the target area percentage was also slightly increased in 24 hours after exercise group, without significant difference (P>0.05). Compared with quiet control group and immediately after exercise group, the target area percentage was increased in 24 hours exercise group (P<0.05). Compared with immediately and 12 hours after exercise groups, the target area percentage was increased significantly in 48 hours after exercise group (P<0.01). Compared with 48 hours after exercise group, the target area percentage began to decline in 72 hours after exercise group (P<0.05), which was higher than quiet control level. After exhaustive eccentric exercise, cytoskeletal vimentin begin to express at varying degrees. The vimentin expression is increased gradual y at 12 hours and reached the peak at 48 hours, then begins to decrease.
2.STUDY OF SERUM IgA ANTIBODY TO EB VIRAL CAPSID ANTIGEN IN NASOPHARYNGEAL CARCINOMA FROM SHAANXI
Journal of Xi'an Jiaotong University(Medical Sciences) 1982;0(04):-
A total of 154 sera from patients with nasopharyngeal carcinoma(NPC), patients with other head and neck tumors(OHNT) and healthy individuals (HI) from Shaanxi province were tested for IgA antibody to EB viral capsid antigen (VCA-IgA) by immunoenzyrnie method. 75.93% of 54 pretreated NPC patients had VCA-IgA antibody with a GMT of 1: 38. 00,whereas less than 12. 00%of 50 OHNT and 50 HI with a GMT of 1: 2. 5 ~ 1: 3. 35.The results showed that the detection of VCA-IgA was of great value in diagnosis of NPC from low risk areas. The elevated level of VCA-IgA was seen in well-differentiated squamous cell carcinoma which gave a pattern similar to that noted in vacuolar nuclear cell carcinoma or less-differentiated carcinoma, but not in undifferentiated carcinoma. The VCA-IgA positive ratio in NPC patients below 30-year-old was similar to that in OHNT, however the GMT was elevated markedly. The results above showed that VCA-IgA antibody was of little value in diagnosis of NPC below 30-year-old and undifferentiated carcinoma.
3.Clinical analysis of combined surgery on 68 patients with refractory glaucoma and coexisting cataract
Xiangdong LIU ; Xiaodong ZHOU ; Xiangping LIU
Journal of Chinese Physician 2012;(z2):19-21
Objective To explore clinical application of the combined therapy of manual small incision cataract surgery (MSICS) with intraocular lens (IOL) implantation and trabeculectomy for patients with refractory glaucoma and coexisting cataract.Methods In our hospital,68 patients (76 eyes) with angle-closure glaucoma of continuously high intraocular pressure (lOP) and coexisting cataract were included in this study from April 2008 to October 2011.All of them were treated with the combination of MSICS with IOL implantation and trabeculectomy.Postoperative visual acuity,IOP,anterior chamber depth (ACD),filtering bleb,ocular fundus and complications were observed.Results Each operation was successfully completed.Postoperative visual acuity and IOP were significantly improved and decreased,respectively,compared to the preoperative.ACD was deepen and filtering bleb was effectively established in the postoperative eyes without significant complications.Conclusion The combined surgery of MSICS with IOL implantation and trabeculectomy might effectively increase visual acuity and decrease IOP,which was a safe and effective approach for patients with refractory glaucoma and coexisting cataract.
4.Changes in cardiac troponin I during extracorporeal circulation
Jimei ZHAO ; Xiangdong LIU ; Chun LIU
Chinese Journal of Laboratory Medicine 2000;0(06):-
Objective To detect the changes in cTnI and CK MB during extracorporeal circulation and the myocardial injury by extracorporeal circulation. Methods The value of cTnI and CK MB was determined in 30 persons during extracorporeal circulation. We divided them into 6 groups according to pre operation, 6, 12, 24, 48 and 72 hours after operation. Modified method (Bodor et al) was used to detect cTnI, and immune suppression method to detect CK MB. Results cTnI and CK MB were, cTnI ( ?s ) (5.20?2 80) ?g/L,CK MB ( ?s ) (10.75?4.70) U/L in 28 persons. They elevated 6 hours after operation. 12 24 hours after operation cTnI and CK MB increased to the maximum, cTnI 28.80 71.30 ?g/L,CK MB 55.60 71.30 U/L. During 48 72 hours, CK MB decreased to normal, 49.70 22.80 U/L. cTnI was 9.40 19.80 ?g/L 72 hours after operation. Conclusion Extracorporeal circulation may cause myocardial diseases. cTnI is a more sensitive biochemical marker for myocardial diseases.
5.The interventional evaluation and management of arteriovenous fistulae in patients with hepatic cancer
Xiangdong LIU ; Chunyu LU ; Ronghua LI
Journal of Interventional Radiology 2009;18(12):934-936
Objective To analyze the DSA manifestations of arteriovenous fistulae in hepatic cancer and to discuss the appropriate method for obstructing the fistulae in order to improve the therapeutic results for hepatic cancer associated with arteriovenous fistulae. Methods The imaging findings in 183 patients of hepatic cancer with urteriovenous fistulae, which were. selected from 637 patients with liver cancer, were retrospectively analyzed. For cases with mild arteriovenous fistulae (n = 89) the tumor-feeding arteries were embolized after iodized oil embolization procedure, while for cases with moderate and severe aneriovenous fistulae (n = 94) the abnormal shunts were obstructed before performing chemoembolization of the tumor in order to ensure that the effective infusion of the drug into the lesion and the sufficient deposit of iodized oil in the tumor could be obtained. Results The tumor-feeding arteries were successfully embolized in all 89 patients with mild arteriovenous fistulae. Of 94 cases with moderate and severe arteriovenous fistulae,excellent deposit of iodized oil was seen in 64 (68.1%), incomplete embolization in 13 (14.83%), poor oil deposit due to over-embolization in 11 (11.7%) and failure to block the fistulae as the fistulae were too large or too diffuse in 6 (6.4%). No serious complications occurred. Conclusion The arteriovenous fistulae accompanied with hepatic cancer can be effectively obstructed in most patients, with resultant clinical improvement of the patients' condition. This therapeutic technique is safe and reliable.
6.Treatment of Unstable Intertrochanteric Fractures in the Eldly with the Prosthetic Artificial Fernord Head
Xiangdong WU ; Peifu TANG ; Lanzhen LIU
Journal of Medical Research 2006;0(09):-
Objective To explore the effect and methods of hip prosthesic replacement of comminuted interochanteric fracture of femur in the eldly.Methods We adopted bone cement hip prosthesic replacements to treat interochanteric fracture of femur in the the eldly.There were 34 cases,with male 11 cases and female 23 cases,aged 74 ~90years old.The fracture was classified according to Evans,with Ⅰa 4 cases,EvansⅠb 12 cases,EvansⅠc 7 cases,EvansⅠd 8cases and EvansⅡ 3 cases.Results All 34 cases got operation successfuly,with the follow-up time of 7 to 48 months postoperatively for all 34 cases.The average hospital stay of the patients was 21 days.The average time to ambulation was 13 days.The incidence rate of the hip joint was 10 percent.Conclusion Bone cement twin exceedingly prosthesic replacement for interochanteric fracture in the the eldly has advmtages of early full weight-bearing and rapid rehabilitation.
7.Terahertz spectroscopy for lung cancer detection: a preliminary study
Dingyuan LIU ; Changcheng SHI ; Xiangdong ZHOU
Journal of Third Military Medical University 2017;39(17):1739-1743
Objective To preliminarily explore the application of terahertz spectroscopy in the early detection of lung cancer by comparing the differences among lung cancer tissues,paracancerous tissues,and normal lung tissues with terahertz spectroscopy and screen the suitable terahertz frequency for specific diagnosis of lung cancer.Methods Thirty groups of lung tissue samples from non-small cell lung cancer patients at stage of Ⅰ to Ⅲ A were collected,and each group contained lung cancer tissue,paracancerous tissues,and normal lung tissue from the same patient.These lung cancer specimens included 17 cases of adenocarcinoma,10 cases of squamous cell carcinoma,2 cases of large cell lung cancer,and 1 case of sarcomatoid carcinoma.Terahertz spectroscopy from 0.2 THz to 2.0 THz was used to detect these tissue samples,and the absorption coefficient was compared.Results ① The absorption coefficient was significantly higher in the lung cancer tissues than the normal lung tissues.② Terahertz spectroscopy at a frequency of 0.8 to 1.4 THz may have the specific diagnostic value of lung cancer.Conclusion Since the absorption rate is higher in the lung cancer than the normal lung tissue,which is suggested that terahertz spectroscopy may be regarded as a novel non-invasive diagnostic approach for lung cancer.The specific diagnostic value of the terahertz frequency may be 1.4-2.0 THz for lung cancer.
8.Serum NO Level and SOD Activity in Patients with Traffic Accident
Guangxiong LIU ; Laiqi YANG ; Xiangdong XU
Chinese Journal of Clinical Psychology 2001;0(03):-
Objective: To explore the stress effects of traffic accident on changing the NO level and SOD activity, as well as the relationship between personality and prognostic change. Methods: Serum level of NO and activity of SOD were measured in 152 patients with traffic accidents and in 60 control subjects. All subjects were assessed with the Eysenck Personality Questionnaire (EPQ). Results: NO and SOD were significantly higher in patients as compared to controls ( P
9.Relativity study during polyethelene debris cytokine and hip prosthesis loosening
Jianbing MA ; Miao LIU ; Xiangdong MENG
Orthopedic Journal of China 2006;0(09):-
0.05),but the integrated arthoplasty group had significant differences from the other two groups(P
10.Stent implantation through butt joint of PTBD and ERCP for the treatment of obstructive jaundice
Yingdi LIU ; Zhiqiang WANG ; Xiangdong WANG
Medical Journal of Chinese People's Liberation Army 2001;0(11):-
Objective To study the technique and to evaluate the clinical value of stent implantation by butt joint of percutaneous transhepatic biliary drainage (PTBD) and endoscopic retrograde cholangio-pancreatography (ERCP) for the treatment of obstructive jaundice. Methods 24 patients with obstructive jaundice were treated by butt joint of PTBD and ERCP after the ERCP failure, and the clinical results were evaluated. Results A total of 24 cases with obstructive jaundice were successfully operated by butt joint of PTBD and ERCP. In PTBD, 10 cases were punctured from right side, and 14 cases were from left side. PTBD, ERCP and butt joint were done simultaneously in 16 cases, and separately in 8 cases. The total serum bilirubin decreased by 47.07 in 4 days, and the direct bilirubin decreased by 45.43. Infection of biliary tract was the main complication. Conclusion Stent implantation by butt joint of PTBD and ERCP for the treatment of obstructive jaundice may be chosen to treat the patients who failed in ERCP alone.