Objevtive To evaluate the efficacy and safety of intensive pulse light (IPL) on treating the telangiectasis and dermal atrophy due to corticosteroid-dependent dermatitis.Methods We applied the IPL on 13 patients who had telangiectasis and dermal atrophy caused by corticosteroid-dependent dermatitis every three to four weeks.The treatment lasted three to eight times.The patients's skin types were Fitzpatrick Ⅲ-V and they had had corticosteroid therapy from 15 months to 13 years.Results To telangiectasis,3 patients had excellent effect afterwards;7 patients achieved fair good effect;Another patients also achieved improvement;2 cases had no positive effect;The total effective rate reached 76.92%.10 patients of dermal atrophy among the total 13 had improvement of the thickness of dermis.Conclusion Intensive pulse light can improve the telangiectasis and dermal atrophy of corticosteroid-dependent dermatitis.It needs less recovery time with fewer side effects and therefore IPL is an effective method for the Iesions.

Objective To explore the effect of aging on nonspatial learning and memory ability or recognitive memory ability in C57BL/6 mice.Method The C57BL /6 mice were used and novel-object recognition task was used to test the recognition memory ability.Results There was hardly exploring behavior in half of mice in the novel-object recognition task.Conclusion The novel-object recognition task is valueless to evaluate the effect of age on the nonspatial learning and memory ability in the C57BL/6 mice.

Objective To explore whether there is the decline of learning and memory ability in C57BL/6 mice in radial six-arm water maze (RAWM) task. Methods The RAWM task was used to test the spatial abilities of learning and memory. Results All of old mice showed the decline of spatial learning and memory ability in the RAWM task. Conclusion RAWM task is excellent spatial learning and memory task and is sensitive to measure the age-related decline of learning and memory ability in C57BL/6 mice.

Objective:Animal models were set up to explore the best preventative intra-nasal administration of interleukin 27 (IL-27)to diminish allergic airway inflammation of asthma and the related molecular mechanisms. Methods:Ninety-six female C57/6J mice were randomly divided into four groups,a group of the control group,a group of asthma group,and two groups of the prevention group. Based on being sensitizing and challenging with Ovalbumin ( OVA ) in the asthma model, two kinds of IL-27 administration asthma animal models were set up,one of which was low-dose-multiple preventive administration before OVA sensitization,one was low-dose-multiple preventive administration after OVA sensitization but before OVA challenge. Sacrificed the mice after challenging and analyzed the IL-5 and IL-13 levels in supernatant of Broncho alveolar lavage fluid( BAL) using ELISA;and HE stain and inflammation score were done for the lungs. Sacrificed the mice before challenging and used the lungs to analyze the level of total signal transducer and activator of transcription-1(STAT1) protein and phos-STAT1 based on the method of Western blot. Results: In low-dose-multiple administration before sensitization preventions group,IL-27 inhibits the secretion of IL-5 and IL-13(P<0. 05) and inflammation around bronchial and vascular obviously, and the inflammation score was lower than asthma group ( P<0. 05 ) , while another group has no significant effects ( P>0. 05 ) . The phosphorylation of STAT1 was impaired in mice after OVA sensitization, and preventative administration of IL-27 before sensitization could reverse the impairment of STAT1, whereas another group had no obviously changes. Conclusion:Preventative administration of IL-27 before sensitization can attenuate the airway inflammation in the mouse asthma model via reversing the phosphorylation of STAT1,while the mice which has been sensitized resisting the inhibition of IL-27 due to the impairment of the phosphorylation of STAT1 and already committed Th2-CD4+T cells existed in sensitization-mice airway might be the reason for such IL-27 resistance.

0.05).[Conclusion]Lateral-anterior fixation with use TSRH not only has effective result in strength, rigidity and stability of the spine, but also is superior to the control group in loading-bearing ability and stability of the spine. There is no significant difference between the TSRH group and Z-plate group. But TSRH is relatively simple to operate and is cheaper than Z-plate, and has exact clinical ontcome.

Cell culture, Fluo-3 AM loading and laser scanning confocal microscope(LSCM), flow cytometer(FCM)and Western blot were employed to detect the regulation effect of retinoic acid on signal transduction pathway of gap junction gene connexin cx43 in HeLa. The results showed that after treated by ATRA , the second intercellular messenger [Ca 2+]i was much higher in HeLa cells(58.16 nmol/L)than in untreated cells(35.73 nmol/L). A detectable and up-regulation of Cx43 in 43kDa protein in HeLa cells increased from 1.9% to 26.3% in RA-treated cells than in untreated cells examined by FCM and Western blot. Immunoblot showed that only the treated cells had phosphorylated Cx43 protein of 43 kDa. The results suggest that the anti-tumor effect of ATRA in HeLa might be due to up-regulation of cx43 gene and its signal transduction pathway which mediates GJIC. The decreased expression of Cx genes, and the disorder and abnormal signal transduction pathway of Cx gene should be responsible for the uncontrolled tumor cell growth.

Objective To compare the effects of different blood purification methods on the removal of large and medium molecular toxins in patients with maintenance hemodialysis(MHD), and to provide the clinical basis for selecting suitable dialysis modalities to prevent or delay the long term complications of uremic patients.Methods60 MHD patients with long term and stable hemodialysis were enrolled in this study.All of them were selected from the patients with insufficient hemodialysis.According to the digital table,60 patients were randomly divided into high flux hemodialysis (HFHD)group and hemodiafiltration (HDF) group,30 cases in each group.And before,3 months and 6 months after dialysis, the serum β2-MG,PTH and Cys C after dialysis were tested.Results The clearance of PTH in the HFHD group[(286.34±127.33)pg/mL] was significantly higher than that in the HDF group[(376.04±141.74)pg/mL],the difference was statistically significant(t=2.45,P<0.05).The clearance of β2-MG in the HDF group[(11.34±1.96)mg/L]was significantly higher than that in the HFHD group[(15.41±3.02)mg/L],the difference was significant(t=5.88,P<0.05).The clearance of Cys C in the HFHD group[(263.67±98.72)μg/dL]was significantly higher than that in the HDF group[(345.63±105.00)μg/dL],the difference was statistically significant(t=2.96,P<0.05).Conclusion Both two dialysis methods are very effective in the removal of large and medium molecular uremic toxins in patients.In the removal of patients with increased PTH,HFHD is more effective.In the removal of patients with increased β2-MG,HDF is more effective.In the removal of Cys C,HFHD is better.

Objective To investigate the role of spinal hyperpolarization-activated cyclic nucleotide-gated (HCN) channels in dexmedetomidine-produced antinociceptic effect.Methods In vivo experiment Thirty wild type C57BL/6J mice and 30 HCN1 gene knockout (HCN1-/-) mice, aged 2-3 months, weighing 19-25 g, were randomly divided into 5 groups (n=6 each) using a random number table: control group (group C), and dexmedetomidine 10, 20, 30 and 40 μg/kg groups (Dex10, Dex20,Dex30 and Dex40 groups).In Dex10, Dex20, Dex30 and Dex40 groups, dexmedetomidine 10, 20, 30 and 40 μg/kg were intraperitoneally injected, respectively.The equal volume of normal saline was given in group C.Before dexmedetomidine administration, and at 15, 30, 45, 60, 75, 90, 105 and 120 min after dexmedetomidine administration, tail flick latency to a thermal nociceptive stimulus was measured, and the percentage of the maximum possible effect (MPE％) was calculated.In vitro experiment HCN1 and HCN2 plasmids and green fluorescent plasmids were transfected into HEK293 cells with liposome 2000.At 24-48 h after transfection, HCN1 and HCN2 channel currents were recorded using whole-cell patch clamp technique.HCN channel currents were recorded as baseline value after rupture of membrane.HEK293 cells were perfused with the extracellular fluid containing different concentrations of dexmedetomidine (0.1, 1.0 or 10.0 μmol/L).After the cells were perfused with dexmedetomidine 0.1 μmol/L for 5 min, HCN currents were recorded.The inhibition rate of currents were calculated.After washout, HCN currents were recorded after the cells were perfused with the next concentration for 5 min.The half-maximal activation voltage (V1/2) of HCN channels and the curve slope were recorded.The difference in V1/2 before and after administration (△V1/2) were calculated.Results Compared with group C, MPE％ was significantly increased in Dex10 group-Dex40 group of wild type and HCN1-/-mice (P＜0.05).Compared with Dex30 and Dex40 groups of wild type mice, MPE％ was significantly decreased in Dex30 and Dex40 groups of HCN1-/-mice (P＜0.05).There was no significant difference in MPE％ between Dex10 group and Dex20 group of wild type and HCN1-/-mice (P＞0.05).Compared with the baseline value, the currents and V1/2 of HCN1 and HCN2 channels in HEK293 cells were significantly decreased when the cells were perfused with dexmedetomidine 0.1, 1.0 and 10.0 μmol/L (P＜0.05).Compared with the value when the cells were perfused with dexmedetomidine 0.1 μmol/L, the currents and V1/2 of HCN1 and HCN2 channels in HEK293 cells were significantly decreased, and inhibition rate of currents and △ V1/2were increased when perfused with dexmedetomidine 1.0 and 10.0 μmol/L (P＜0.05).Compared with the value when the cells were perfused with dexmedetomidine 1.0 μmol/L, the currents and V1/2 of HCN1 and HCN2 channels in HEK293 cells were significantly decreased, and inhibition rate of currents and △ V1/2were increased when perfused with dexmedetomidine 10.0 μmol/L (P＜0.05).There was no significant difference in the activation curve slope of HCN1 and HCN2 channel currents in HEK293 cells when the cells were perfused with dexmedetomidine 0.1, 1.0 and 10.0 μmol/L (P＞0.05).Conclusion Dexmedetomidine-produced antinociceptic effect is likely related to the inhibition of spinal HCN channel opening.

Objective To analyse the effect of different blood purification methods on serum cardiac troponinc T (cTnT), cardiac troponinⅠ(cTnI), β2-microglobulin (β2-MG) and interleukin-6 (IL-6) levels in patients with maintenance hemodialysis.Methods 60 uremic patients treated with maintenance hemodialysis were collected.All patients were randomly divided into conventional hemodialysis group (HD group) , hemodialysis and hemoperfusion group (HD+HP group) and hemodiafiltration group (HDF group) , 20 cases in each group.Corresponding dialysis treatment was given, then the serum levels of cTnT, cTnI,β2-MG and IL-6 were detected in all patients post-treatment.Results After treatment, the serum cTnT, cTnI,β2-MG and IL-6 levels in HD+HP group and HDF group were lower than those in HD group (P<0.05).Conclusions Different blood purification methods have different effects on serum cTnT, cTnI, β2-MG and IL-6 levels in patients with maintenance hemodialysis, HDF and HD +HP have better scavenging effect, which has the guiding significance to clinical application.

Objective To investigate the effects of exposure of gravid rats to the persistent organic pollutants (POPs), methoxychlor (MXC), on the placenta and their progeny during gestation periods. Methods According to different dosage levels of MXC [16, 32, 64 and 0 mg/(kg?d)], forty female SD rats aged 3-month were randomly divided into low-dosage group, mid-dosage group, high-dosage group and control group, with 10 animals for each. All the rats received intraperitoneal injection of MXC for 20 days. The influence of MXC on placenta of gravid rats and their progeny were observed in all aspects. Results With the increase in MXC dosage, intense changes were found in the rats, including an inerease in the number of corpus luteum of ovary, the number of nidation, fetal death and merging fetal (P