1.Construction of a 5-Lipoxygenase Transgenic Mice
Meiying ZHANG ; Honglian WU ; Wei YANG ; Zhaoyang LI ; Wanwei DONG ; Shenglai ZHOU ; Yang YU ; Wei WANG ; Xiangchuan Lü ; Ying QIN ; Zhihong ZHENG ; Luzeng WANG
Acta Laboratorium Animalis Scientia Sinica 2010;18(1):60-64
Objective To construct a 5-lipoxygenase (5-LO) transgenic mouse model of atherosclerosis.Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice.PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues.RT-PCR and Western blot analysis were used to detect the gene transcription and expression.Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.Expression of 5-LO and FLAP was found in the bone marrow,spleen,kidney,and peritoneal cells.Results of RT-PCR and Western blot showed that No.9,20,24transgenic mice expressed a higher level of 5-LO and FLAP than those in the wild type C57BL/6 mice.The expression levels in bone marrow and peritoneal cells were significantly different(P<0.05).Conclusion A 5-LO transgenie mouse line has been established in this study and may be used for future study on the function of 5-LO gene.
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