Objective The aim of the paper is to investigate the alteration of the physical-chemical properties and disinfection effect of electrolyzed-oxidizing water during storage, and to provide theory support for its application. Methods A typical electrolyzed-oxidizing water was stored under a closed and light condition for 66 days and its physical-chemical parameters and disinfection effect were measured at fixed periods. Results The results showed that the chlorine loss of the electrolyzed-oxidizing water was quite fast and reached 50% and 100% after 10 days and 66 days of storage period respectively, the pH value was quite stable, from 2.5 to 2.47. The oxidation-reduction potential(ORP) of the water decreased slowly only from 1 146 mV to 1 100 mV within 45 day, but decreased rapidly after 45 days of storage. The results also showed that the inactivation of Bacillus subtilis var niger spores by the electrolyzed-oxidizing water also decreased rapidly after one week of storage,from 100%(the first week) to less than 90%(after 10 days). Conclusion The electrolyzed-oxidizing water can keep its strong disinfection effect after 7-10 days of storage. It was believed that the chlorine loss from the electrolyzed-oxidizing water was the main reason for the decrease of its disinfection effect during storage.

Ulcerative colitis （UC） is one of the chronic refractory inflammatory bowel diseases characterized by abdominal pain， diarrhea， and mucus， pus and blood in the stool. In recent years， with changes in human life style and improvements of the diagnosis， the incidence and prevalence of UC have been increasing. The pathogenesis of UC is closely related to intestinal mucosal immune dysfunction， intestinal flora disturbance， and abnormal bile acid secretion. Patients with UC have abnormal bile acid secretion and intestinal flora imbalance. A large number of studies have found that abnormal bile acid secretion inhibits immune function， affects signal transduction， and destroys the intestinal mucosal barrier. Intestinal flora disturbance has an important impact on the occurrence and development of inflammation， immune homeostasis， and stress. Bile acids indirectly or directly affect the structure and function of intestinal flora， and at the same time， they produce secondary bile acids under the modification of intestinal flora， entering the liver through enterohepatic circulation. Therefore， the complex dialogue mechanism of bile acid-intestinal flora axis is closely related to the occurrence and development of UC. Based on the basic theory of traditional Chinese medicine（TCM） and clinical research， it is found that emotion is an important factor that induces this disease， spleen and stomach weakness is the root of the disease， and liver depression and spleen deficiency are the key pathogenesis of UC. Combined with modern medicine and molecular biology research， it is believed that abnormal secretion of bile acids is a microscopic manifestation of liver depression in TCM， and intestinal flora disturbance is the biological basis of spleen deficiency. In the pathogenesis of UC， the imbalanced bile acid-intestinal flora axis is consistent with the pathogenesis of liver depression and spleen in TCM. The exploration of the biological connotation of the pathogenesis of UC with liver depression and spleen deficiency from the perspective of bile acid-intestinal flora axis can better explain the scientific nature of its pathogenesis， which provides new clinical solutions and reliable references for studying the pathogenesis of UC with liver depression and spleen deficiency and finding representative prescriptions to prevent and treat this disease.