BACKGROUND:Studies have shown that Ganoderma lucidum polysaccharides have biological activities of anti-viral, anti-tumor, enhance immunity, anti-oxidative and anti-aging. OBJECTIVE:To observe the biological effect of Ganoderma lucidum polysaccharides on the antioxidant capacity of D-galactose induced aging mice skin tissues, and to observe the effect in postponing skin aging. METHODS:Forty-four 2-month-old Kunming mice were randomly divided into four groups:normal control group, aging model group, vitamin E group, and Ganoderma lucidum polysaccharide group. Rats in the last three groups received subcutaneous injection (nape area) of Ganoderma lucidum polysaccharide to establish rat aging models, and intragastric administration of corresponding drugs or saline was performed. After 42 days, pathological sections of back skin were obtained to assess the morphological changes of skin tissues, measure the epidermal and dermal thickness, and detect the superoxide dismutase levels and the expression of CuZn-superoxide dismutase mRNA. RESULTS AND CONCLUSION:The epidermal and dermal thickness in the vitamin E group and Ganoderma lucidum polysaccharide group were increased when compared with the aging model group. The superoxide dismutase levels in the Ganoderma lucidum polysaccharide group were significantly higher than those in the other two groups. The decreased degree of cycle threshold value in the Ganoderma lucidum polysaccharide group was lower than that in the other two groups. The results indicate that Ganoderma lucidum polysaccharide can increase the epidermal and dermal thickness, improve the skin tissue structure, and enhance the superoxide dismutase levels and the expression of CuZn-superoxide dismutase mRNA.

AIM:To investigate the process and advantages of wound repair at one stage after repair of nasal skin defect with bilobed flap.METHODS:Under local infiltration anesthesia,nasal skin defects were repaired by bilobed skin flaps.The normal skin around the defect was designed for bilobed island skin flaps.The maximum size of the flaps was 1.8 cm?2.5 cm,and the minimum size was 0.8 cm?1.0 cm.And then the flaps were transferred to repair the defects.The distances from incisions to margin of lesions were as follow:hemangioma was 0.2 cm,basal cell carcinoma was 0.5-0.7 cm,and melanoma was 1.0 cm.The depths of incisions reached superficial fascia layer.Complete hemostasis of wound was performed.RESULTS:Fifty of 92 patients were followed up for 6 months,and 42 of them were followed up for 12 months.In all patients,the nasal skin defects were repaired completely with primary healing.The color and texture of the flap were the samilar with the adjacent skin,and the nose contour and cosmetic results were satisfactory.CONCLUSION:Repair of bilobed island skin flap for nasal skin defeets was simple,and the efficacy was satisfactory.

Resection of huge hepatic tumor located at segments Ⅶ and Ⅷ with reservation of segments Ⅴ and Ⅵ is difficult,because the right hepatic vein is easy to be injured during the operation.A male patient with huge hepatic tumor in the right lobe was admitted to the Cancer Hospital of Guangxi Medical University on July 26,2012.The tumor (diameter:14 cm) was located at segments Ⅶ and Ⅷ.The tumor and the whole structure of the liver were three dimensionally reconstructed by using digital medical technology and liver surgery planning system.The right hepatic vein was closely adjacent to the tumor.During the operation,the right Glisson pedicles were occluded intermittently,the liver parenchyma was dissected with cavitron ultrasonic surgical aspirator,and the right hepatic vein was occlued with non-invasive vascular clamp.The tumor was resected completely,with successful preservation of the trunk of right hepatic vein.No blood transfusion was needed,and the blood supply to segments Ⅴ and Ⅵ was not influenced.The patient recovered rapidly and was discharged on postoperative day 14.

OBJECTIVE:To establish the quality standard of Oleanolic acid dripping pills. METHODS:The property of the preparation was identified,and weight difference and dissolution time limit were detected. UPLC method was adopted to determine the content of oleanolic acid in the preparation. The determination was performed on ACQUITY UPLC BEH C18 column with mo-bile phase consisted of acetonitrile-water (70:30,V/V) at the flow rate of 0.30 mL/min. The detection wavelength was set at 206 nm,the column temperature was 30 ℃,and the sample was 5 μL. RESULTS:The characteristics of the preparation was significant;weight difference ranged 37.62%-46.56%;dissolution time limit was 24 min. Linear range of oleanolic acid ranged 0.006-0.06 mg/mL (r=0.9998). RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 99.34%-100.40%(RSD=0.4%,n=6). CONCLUSIONS:Established standard can be used for quality control of Oleanolic acid dripping pills.

Objective To observe the relationship between the changes of plasma D dimer in pathogenic course and prognosis in patients with acute cerebral infarction(ACI). Methods Changes in plasma D dimer levels of 93 patients with ACI and 20 cases healthy persons were detected dynamically by Latex semi quantitative method. The relation between D dimer levels and focus size, severity of infarction and prognosis were analyzed. Results There was significant difference( P

Baculoviruses produce two viral phenotypes, the budded virus (BV) and the occlusion-derived virus (ODV). ODVs are released from occlusion bodies in the midgut where they initiate a primary infection. Due to the lack of an in vitro system, the molecular mechanism of ODV infection is still unclear. Here we present data demonstrating that Helicoverpa armigera nucleopolyhedrovirus (HearNPV) ODV infected cultured Hz-AM1 cells in a pH dependent manner. The optimal pH for ODV infection was 8.5, which is same to that in the microvilli of midgut epithelial cells, the ODV native infection sites. Antibodies neutralization analysis indicated that four HearNPV oral infection essential genes p74, pif-1, pif-2 and pif-3 are also essential for HearNPV ODV infection in vitro. Thus, HearNPV-HzAM1 system can be used to analyze the mechanism of ODV entry.

Objective To explore the effect of quality control circle (QCC) on reducing the incidence of complications in the patients with peritoneal dialysis (PD). Methods Eighty-nine patients who underwent peritoneal dialysis after discharge were divided into QCC group (45 cases) and control group (44 cases) according to random sampling method. Both groups were followed up regularly, QCC activity was applied in patients of the QCC group on the basis of the QCC activity procedure. The intervention time was 1 year. The blood pressure, standard rate of urea clearance index, peritonitis incidence, catheter drift rate, infection incidences of external orifice of catheter, admission rate of complications and peritoneal dialysis withdrawal incidence between 2 groups were compared. Results After interference, the systolic and diastolic blood pressure in QCC group were significantly lower than those in control group:(124.11 ± 13.07) mmHg (1 mmHg=0.133 kPa) vs. (132.68 ± 12.09) mmHg and (72.13 ± 10.51) mmHg vs. (78.91 ± 11.79) mmHg, and there were statistical differences (P<0.05). The standard rate of urea clearance index in QCC group was significantly higher than that in control group:95.56%(43/45) vs. 81.82%(36/44), and there was statistical difference (P<0.05). After interference, the peritonitis incidence and admission rate of complications in QCC group was significantly lower than those in control group: 2.22% (1/45) vs. 13.64% (6/44) and 0 vs. 9.09% (4/44), and there were statistical differences (P<0.05). There were no statistical differences in catheter drift rate, infection incidences of external orifice of catheter and peritoneal dialysis withdrawal incidence between 2 group (P>0.05). The Spearman correlation analysis result showed that peritonitis was negatively correlated with education level (r = -0.28, P < 0.05), and standard rate of urea clearance index was positively correlated with education level (r = 0.21, P < 0.05). Conclusions The QCC management model can reduce the incidence of complications in patients with peritoneal dialysis.

Objective To establish a tissue culture system of Pueraria thomsonii and to cultivate a new breed autotetraploid.Methods Using the MS medium as the basic medium,the explants shoot apexes and stem segments were cultured to differentiate the regenerations.The autotetraloid with genetic material doubled was achieved by treating shoot tips with colchicine of different concentrations for different long times.Results The proper media MS+6-BA 1.0 mg/L + 2,4-D 1.0 mg/L+NAA 0.2 mg/L is for pro-(tocorn) inducing,MS+6-BA 1.0 mg/L+2,4-D 0.2 mg/L for diffrentiation and propagation,and 1/2(MS+) IBA 0.2 mg/L for rooting.The best effect of the autotetraploid induction can be achieved by treating the 0.3-0.5 cm shoot tips cut from the 1 cm plantlets with 0.4%-0.5% colchicine for 48 h,and it is unlikely to succeed by treating shoot tips with colchicine of very low or high concentration or for very long or short time.Conclusion The autotetraploid gives a chance to improve the content of medical materials in the root of P.thomsonii.

30 ml) undergoing minimally invasive evacuation of intracranial hematoma from January 1999 to October 2002 in our department were analyzed retrospectively. The changes of IL 1, IL 6, and IL 8 in hematoma fluid were observed continuously. The content of IL 1 was determined by radioimmunassay and IL 6, IL 8 by ELISA methed. Results IL 1, IL 6, and IL 8 were observed at 6 12 h after hypertensive cerebral hemorrhage, and showed different changes in acute stage. Conclusion Inflammatory cytokines are involved in the pathological process of cerebral hemorrhage.

Adult ; Humans ; Laryngostenosis ; surgery ; Larynx ; injuries ; Male ; Trachea ; injuries ; Tracheal Stenosis ; surgery ; Wounds and Injuries ; surgery