Endothelial monocyte-activating polypeptide-Ⅱ (EMAP-Ⅱ ) is a novel proinflammatory cytokine with proinflammatory,proapoptotic and antiangiogenic properties.It is associated with many tumorassociated proteins,such as tumor necrosis factor,vascular endothelial growth factor,hypoxia inducible factor-1α,arginyl-tRNA synthetase,and insulin-like growth factor- Ⅰ.EMAP-Ⅱ has anti-tumor properties and has a good prospect in cancer prevention and treatment.

Objective:To investigate the different function of dendritic cells between patients with lupus nephritis and healthy control,and discuss the pathogenesis of the disease.Methods: Choose 50 healthy controls,50 patients with lupus nephritis,and isolation of blood dendritic cells.Then samples were induced with GM-CSF and IL-4,and cells were collected on day 7.DC maturity was evaluated using the following methods:fluorescence-activated cell sorting analysis for cell surface markers;enzyme-linked immunosorbent assay for cytokine production;cell proliferation assay for induction of T cell proliferation.Results: Compared with the control group,lupus nephritis group had increased MHC Ⅱ,CD86,CD80,and CD40 expression(P<0.05);displayed the increased IL-12 and TNF-αlevels(P<0.05);displayed a increased ability to drive lymphocyte cells proliferation (P<0.05).Conclusion:Compared with the control group ,dendritic cells in lupus nephritis group displayed the mature condition ,which indicates that dendritic cells play the important role in the pathogenesis of lupus nephritis.

Objective To evaluate the effectiveness of periodontal mechanical therapy on periodontal health and interleukin-6 (IL-6) levels in gingival crevicular fluid (GCF) in type 2 diabetic patients with chronic periodontitis. Methods GCF samples were obtained with filter papers in first molar sites from 37 type 2 diabetic patients with chronic periodontitis. The randomized controlled and blinded clinical trial with a duration of six months was designed to compare effects between three groups of professional mechanical tooth cleaning (PMTC), i.e. coronal scaling (group Ⅱ) with periodontal initial therapy (group Ⅰ) and without clinical therapy (control group). The clinical periodontal index such as probing depth (PD), attachment loss (AL), modified bleeding index (mBI) and bacteria plaque index (PLI) were obtained with a Williams type periodontal probe. Laboratory examination including GCF volume, concentrations and total amounts of IL-6 in GCF were detected with ELISA method. Results Significantly decreasing trends of PD, AL, mBI, GCF volume and total amounts of IL-6 were observed in group Ⅰin all test period. The group Ⅱ had a significant reduction of PD at the third month as compared with baseline (⊿=0.36 mm, P＜0.05), and the other figures showed descending trends but didn′t present statistical significances. At the end of study, the group Ⅰand Ⅱ had significant reductions of GCF volume compared with increases of control group (0.96 μ1＞0.03 μ1mm＞-0.20 μ1, P＜0.05). Conclusions The sequential periodontal supra- and sub- gingival scaling has definitive effects on periodontal health improvement and on reducing the IL-6 level in GCF in type 2 diabetic patients.

Objective To investigate the association between serum non-organ-specific autoantibodies and polycystic ovary syn-drome(PCOS).Methods A total of 69 patients with PCOS in child-bearing period treated in our hospital were selected as the study group and contemporaneous 69 healthy child-bearing year women were selected as the control group.Serum level of antinuclear anti-bodies (ANA)was measured by ELISA,serum level of anti-dsDNA was measured by colloidal gold spot infiltration assay and the extractable nuclear antigen (ENA)auto-antibodies profiles were measured by Western blot.Results Serum levels of ANA and an-ti-dsDNA antibodies in the study group were significantly elevated compared with the control group with statistical difference.Con-clusion Serum autoantibody positive exists in the patients with PCOS.

0.05). The data proved that PSMA7 was overexpressed in pcDNA3.1(-)/PSMA7-transfected A549 cell line, both in mRNA level and in protein level. Conclusion Eukaryotic expression plasmid pcDNA3.1(-)/PSMA7 has been successfully constructed, and the PSMA7 is stably expressed in A549 cell line. This would pave the way for further study of PSMA7.

Objective To locate the brain areas in which pain was induced by mechanical noxious stimulation by using functional magnetic resonance imaging.Methods Twenty healthy male volunteers,aged 20-40 yr,with body mass index of 18-25 kg/m2,were involved in this study.The volunteers were stimulated with 300 g von Frey filaments.Functional magnetic resonance imaging examinations were performed 1 week later.The monitoring data were collected during the scanning.The images were analyzed with SPM2 software.Results Bain areas in which pain was induced by mechanical noxious stimulation with 300 g yon Frey filaments were bilateral anterior cingulate gyrus,right contralateral insula and bilateral primary somatic sensory cortex.Conclusion The brain areas in which pain is induced by mechanical noxious stimulation include bilateral anterior cingulate gyrus,right contralateral insula and bilateral primary somatic sensory cortex.

Objective To investigate the effects of different doses of fentanyl on pain-activated brain areas as demonstrated by functional magnetic resonance imaging (fMRI) at 3.0 T.Methods Twenty healthy right-handed male volunteers aged 20-40 yr were randomly divided into 2 groups ( n =10 each); group F1 (fentanyl 1.0 μg/kg) and group F2 (fentanyl 1.5 μg/kg).Mechanical stimulation with von Frey filaments (vFFs,300 g) was delivered to left sole.The intensity of pain was assessed by VAS scores.fMRI was performed before and after fentanyl administration and the changes in the brain areas activated by pain were recorded.Results In group F1 ipsilateral (left) cingulate gyrus was activated after a bolus of fentanyl 1.0 μg/kg under stimulation with vFFs 300 g,while in group F2 bilateral cingulate gyrus and contralateral (right) insula were activated under vFFs stimulation after fentanyl 1.5 μg/kg.Conclusion Cingulate gyrus and insula may be the target brain areas of fentanyl analgesia.

Objective To compare the efficacies of extended and standard radical pancreatoduodenectomy (RPD) for the treatment of pancreatic cancer in head of pancreas.Methods The clinical data of 96 patients with pancreatic cancer in head of pancreas who were admitted to the West China Hospital of Sichuan University from 2001 to 2011 were retrospectively analyzed.Forty-one patients who received extended RPD were in the extended group and 55 patients who received standard RPD were in the standard group.Blood loss,operation time,hospital stay,surgical complications,severity of complication,1-,3-,5-year survival rate,median survival time,time and location of tumor recurrence and 1-year quality of life of the patients in the 2 groups were compared.All data were analyzed by using rank sum test and analysis of variance.The survival rate was calculated by Kaplan-Meier method and was analyzed by Log-rank test.Results There were no significant differences in the age,diameter and differentiation of the tumor,and number of metastatic lymph nodes between the 2 groups.The operation time,operative blood loss,postoperative hospital stay were 364 minutes (310-650 minutes),680 ml (0-1800 ml) and 13.5 days (10-76 days) in the extended group,which were significantly higher than 315 minutes (260-306 minutes),305 ml (0-1000 ml) and 9.2 days (7-30 days) in the standard group (F=7.15,4.22,3.82,P＜0.05).The incidences of complications in grade 1 and 2 were 5％ (2/41) and 20％ (8/41) in the extended group,which were significantly lower than 25％ ( 14/55 ) and 49％ (27/55) in the standard group ( x2 =5.76,8.87,P ＜ 0.05).The incidence of complications in grade 3a was 41％ ( 17/41 ) in the extended group,which was significantly higher than 7％ (4/55) in the standard group ( x2 =14.10,P ＜ 0.05 ).There were no significant differences in the incidence of complications in grade 3b and 4a between the 2 groups ( x2 =1.44,0.88,P ＞ 0.05 ).There were no significant difference in the 1-,3-,5-year survival rate,median survival time,postoperative quality of life and time of tumor recurrence between the 2 groups ( x2 =0,0.13,0; F =0.49,1.03,t =0.32,P ＞ 0.05 ).Conclusion Standard RPD is optimal for patients with ordinary pancreatic cancer in head of pancreas.Extended RPD could be applied for patients with enlargement of group 16 lymph nodes and could receive R0 resection.

[Objective] The aim of the present study was to investigate the role of membrane estrogen receptor (mER) mediated pathway in the proliferation and apoptosis of endothelial progenitor cells (EPCs). [Methods] Bone marrow (BM)-derived EPCs were cultured. The cells were divided into different groups, plus or not plus estrogen receptor blocker (ICI 182,780), PI3K inhibitors (LY294002), and NOS inhibitor (L-NAME) to show the effect of E_2-BSA on EPCs. The proliferation of EPCs was determined by MTT and nitric oxide (NO) release was measured by chromatometry. Apoptotic cell death was determined using the Hochest 33258 staining. The expression of phosphorylated eNOS (p-eNOS) were detected by Western blot. [Results] E_2-BSA could increase EPCs proliferation, and this effect was inhibited by estrogen receptor blocker ICI 182,780, thus indicated that mER-initiated membrane signaling pathways were involved in the action of estrogen on EPCs. E_2-BSA increased nitric oxide production and inhibited apoptosis induced by serum withdrawal, and this effect also inhibited by PI3K inhibitor (LY294002), NOS inhibitor (L-NAME)and estrogen receptor blocker(ICI 182,780), thus indicated that PI3K/Akt/NO pathway was involved the effect of estrogen on EPCs apoptosis. Moreover, E_2-BSA treatment increased phosphorylation of eNOS (p-eNOS). PI3K inhibitors (LY294002) also blocked these effects. [Conclusions] The results of present study suggested that mER mediated EPCs proliferation and apoptosis were related to the PI3K/Akt/eNOS pathway.

Objective To evaluate the factors influencing the reproducibility and validity of food group intakes measured by a food frequency questionnaire (FFQ) for Chinese men. Methods A total of 214 adult men were randomly selected from the Shanghai Men Health Study (SMHS), an ongoing cohort study conducted in urban Shanghai. Each subject who had completed the first FFQ survey at recruitment took 24-hour dietary recall (24-HDR) surveys which were conducted once a month for 12 consecutive months and a second FFQ at the end of the study. Two FFQs were administered approximately 1.2 years apart. The food items listed in FFQ and obtained from 24-HDR were categorized into 11 food groups. The factors influencing the reproducibility of the FFQ was assessed by comparing the Pearson correlation of intake levels from the two FFQs, while the factors influencing the validity was evaluated by comparing intake levels from the second FFQ with the multiple 24-HDRs. Results A total of 195 men completed all 12 times of 24-HDR and twice FFQ surveys. The median Pearson correlation coefficients of the food groups between the two FFQs in older vs younger age group were 0.62 vs 0.50;in higher vs lower education level group were 0.58 vs 0.50;in higher vs lower income group were 0.51 vs 0.50;in higher vs lower BMI group were 0.50 vs 0.49. The median Pearson correlation coefficients of the food groups between the second FFQ and 24-HDRs in older vs younger age group were 0.61 vs 0.45;in higher vs lower education level group were 0.49 vs 0.47;in higher vs lower income group were 0.43 vs 0.57;in higher vs lower BMI group were 0.48 vs 0.51. Conclusions Age is the main factor influencing the reproducibility of the food group intakes by a FFQ used in the SMHS, while the factors influencing the validity are age and family income.