The author treated forty-eight cases of infantile cerebral palsy with scalp acupuncture and point injection. After two courses of treatment the infants made progress in different degrees. Eight cases were cured,sixteen cases were obviously improved, twenty-two cases were improved and two cases were unchanged. The total effective rate was 95.8%.

Aim To investigate the effect of ranolazine postconditioning on myocardial apoptosis, the reperfusion injurysalvage kinase and the opening of MPTP in isolated rat hearts subjected to ischemia and reperfusion.Methods The langendorff mode was set up.Fifty-five SD rat hearts were randomly divided into 8 groups (n=7): ischema and reperfusion (I/R group),20 μmol·L~(-1) ranolazine postconditioning group(RPostC),ranolazine and the specific MPTP opener atractyloside group (RA), ranolazine and the specific ERK1/2 inhibitor PD98059 group (RP), ranolazine and the specific PI3K inhibitor PD98059 wortmannin group(RW), atractyloside group(A), PD98059 group (P) and wortmannin group (W). The isolated hearts were subjected to 30 minutes ischemia,10minutes drug postconditioning and 5 mitutes K-H buffer reperfusion.Myocardial apoptosis (TUNEL-positive cells), apoptotic index (AI), the opening of MPTP (spectrophotometry) and the The expression of p-AKT and p-ERK1/2 protein were measured at the end of reperfusion in each group.Results Compared with IR group, Myocardial AI and opening of MPTP were decreased in RPostC groups (P<0.05), the expression of p-AKT, p-ERK1/2 protein were increased in RPostC, RA, RP, and RW groups (P<0.05).There were no significant differences in other groups(P>0.05). Compared with RPostC group, Myocardial AI and opening of MPTP were increased in RA, RP, RW, A, P and W groups (P<0.05), and the expression of p-AKT、p-ERK1/2 protein were decreased in RP, RW, A, P and W groups (P<0.05). There were no significant differences of the expressions of p-AKT,p-ERK1/2 protein in RA groups (P>0.05). Compared with RA group, there were no significant differences in myocardial AI and opening of MPTP (P>0.05) and the expression of p-AKT and p-ERK1/2 protein were decreased in RP, RW, A, P and W groups (P<0.05).Conclusions Ranolazine postconditioning can attenuate myocardial apoptosis in isolated rat hearts subjected to ischemia and reperfusion via activation RISK pathway and inhibition of the opening of MPTP.

Alkanes ; adverse effects ; Empyema ; chemically induced ; complications ; Humans ; Male ; Middle Aged ; Pneumonia ; chemically induced ; complications

Acupuncture Points ; Acupuncture Therapy ; history ; China ; History, 20th Century ; History, 21st Century ; History, Ancient ; Humans ; Medicine in Literature

Objective To study the clinical application oflaparoscopic-assisted operation to colorectal neoplasms. Methods 28 cases of colorectal neoplasms underwent laparoscopic surgery from July 1997 to November 2000. The mean age was 65.3 years old(33～89)years.3 cases underwent right hemicolectomy.1 case of sigmoid colon adenoma was given partial colectomy. Sigmoid-rectal anterior resection was conducted in 21 cases. 3 cases underwent abdominal peritonieal resection. Results 5 cases were converted to open laparotomy.The mean operative time was 178(150～300)min for 23 cases given laparoscopic surgery with 135(30～1000)ml of average intraoperative bleeding. Neither postoperative complications nor intraoperative deaths occurred.1 case of low portion rectal cancer showed abdominal metastasis 12 months after surgery.Neither port site nor incision metastasis happened. Conclusions Laparoscopic assisted surgery has the advantages of less surgical trauma,less gastrointestinal interference and quicker recovery. Under the circumstances of radical resection and selected candidate,laparoscopic assisted surgery can be applied to colorectal neoplasms.

Objective To investigate the infection of chlamydiae trachomahs(CT) and myoplasmas in urogenital tract and antibiotic susceptibility of cultured genital myoplasmas.Methods 5095 patients with urogenital tract infection were detected with mycoplasma identification susceptibility testing reagent kit,and the drug susceptibility to eight antibiotics of ureaplasma urealyticum (Uu) and mycoplasm hominid (Mh) were tested by broth microdilution method.And chlamydiae trachomatis was examined by golden standard method.Results In 5095 cases, 417cases(8 2%) were infected with chlamydiae trachomatis, and the infective rate in woman(11 2%) was statistically higher than that in man(6 2%). 1728 cases(33 9%) were infected with mycoplasma, and the infective rate in woman(43 0%) was statistically higher than that in man(28 0%).The cases infected with simple UU(1251,24 6%) were more than that in the cases infected with simple Mh(71,1 4%) and the mixed infected cases(406,8 0%). Drug sensitivity to erythromycin, roxithromycin, josamycin, azithromycin, doxycycline, minocin,ofloxacin, ciprofloxacin in Uu infection were 60 3%,67 5%,73 2%,85 3%,55 7%,40 1%,25 6%,2 7%,respectively;while the mixed infection of Mh and Uu had resistance to the eight antibiotics on the different degree.Conclusions The infective rate of chlamydiae trachomatis and myoplasma in urogential tract and the resistance rates to 8 antibiotics in Hunan province were in a higher level, compared with other area inland. It is necessary to develop antibiotic susceptibility test,in addition to the myoplasma culture for guiding the clinical therapy.

Objective The changes of cytokine expression on a genomic scale in CD4 + cell of mice c hronically infected with Schistosoma japonicum and their roles in the pathog enesis of schistosomiasis were studied. Methods CD4 + cells were isolated from sp le ens of mice 13 wk infected with Schistosoma japonicum. We used the cDNA micr oarr ay technique to explore the cytokine gene expression profile of CD4 + cells f ro m normal and chronic infected mice. Three-color flow cytometry was performed to study intracellular protein levels of IFN-?、IL-4 of CD4 + cell. Results In the CD4 + cell of chronically infected mice, IL-4 was remarkably increase d at both transcription and protein expression levels (P

Objective: To establish a method for determination of emodin in rat plasma by HPLC/Q-Exactive HR/MS, and to study the pharmacokinetics of emodin in normal rats and cerebral ischemia rats. Methods: The plasma concentration of emodin was determined by HPLC/Q-Exactive HRMS method with internal standard method. Emodin was eluted on a XBridgeTM C18 (150 mm × 2.1 mm, 5 μm) column with temperature at 30 ℃. The mobile phase consisted of 3 mmol/L ammonium acetate and methanol, with a gradient program as follows: 0~2 min (30% methanol), 2-10 min (30%-60% methanol), 10-13 min (60%-30% methanol). The flow rate was 0.3 mL/min, and the injection volume was 5 μL. MS experiments were coupled with the HPLC via HESI source operated in negative ionization full-scan mode. The pharmacokinetic parameters were calculated by the software of DAS 3.0. Results: The main pharmacokinetic parameters of emodin in normal rats and cerebral ischemia rats were as follows: AUC0-∞ were (605.63 ± 163.66) and (1 107.78 ± 191.11) ng∙h/mL, Cmax were (81.96 ± 20.72) and (91.65 ± 16.82) ng/mL, VZ/F were (851.03 ± 97.30) and (1 051.87 ± 119.88) L/kg, t1/2 were (10.31 ± 1.61) and (23.13 ± 3.56) h, tmax were (0.75 ± 0.22) and (0.75 ± 0.16) h. Conclusion: The method is simple, accurate, fast, sensitive, and suitable for the pharmacokinetic study of emodin in rats.

Objective: To establish an HPLC fingerprint method of Verbenae officinalis L. and determine the contents of its main components. Methods: Many batches of Verbenae officinalis fingerprint and the contents of main components were determined by HPLC, and the similarity and cluster analysis of the fingerprints of each batch were compared and the contents of the main components were compared. Results: The results showed that the difference of the main content of medicinal materials and fingerprints were between each batch of Verbenae officinalis. Conclusion: The fingerprint and its main components determination method have been established to provide the scientific basis for the quality evaluation of Verbenae officinalis.

Objective: To establish the HPLC fingerprint of herbs of Patriniae Herba. Methods: The fingerprints of Patriniae Herba were built using Orca C18 column and acetonitrile-0.05% phosphoric acid as mobile phase. The flow rate was 1.0 mL/min. The detecting wavelength was set at 230 nm. The temperature of column was at 35℃. Results: Under the selected spectrum conditions, HPLC fingerprint of herbs of Patriniae Herba was established, and eight public peaks were shown in the HPLC fingerprint. The methodological study met the required standards. Cluster analysis showed that class I medical BJ1, BJ2, BJ3, BJ4, BJ5, BJ6, BJ7, BJ8, BJ9, both, BJ13, BJ14 each batch Patriniae Herba and control the similarity between fingerprints was 0.987-0.907, Indicating that there are good consistency between batches of medicinal materials; class II medical BJ11, BJ12, and BJ15 group of Patriniae Herba and control fingerprint differences larger. Conclusion: The method is accurate and reliable, simple and efficient, and can be used as the evaluation of Patriniae Herba from different origins and with different varieties.