Purpose:To investigate the relationship between the expression of NF?B family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell lines. Methods:First, 13 ovarian cancer cell lines were cultured, and then protein was extracted separately from cytoplasm and nucleus. Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NF?B family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.Results:It was found that the expression of positive rate of P65, P50 and IKB in cytoplasm was 76.9%, 81.8% and 84.6% respectively, and the significant positive expression of P65 and P50 was also found in the nucleus, with a rate of 15.3%, 45.5% respectively. In OV MZ 5 and OV MZ 2774, the expression of P65 was positive and their IC 50 reached a significant value. Conclusions:It was concluded that P50 and P65 affected the growth and development of ovarian cancer cells with the effect of P50 being more obvious. P65 had a close relationship with the chemotherapy resistance of ovarian cancer cells, and thus P65 could be expected to be a new marker in the observation of prognosis.

Aortic Valve ; surgery ; Cardiac Catheterization ; Heart Valve Prosthesis Implantation ; methods ; Humans

Cardiac Catheterization ; standards ; Heart Defects, Congenital ; therapy ; Humans

Now medical undergraduates are faced with a series of problems in the clinical practice.Instructors should work well on students' education work to improve the effect and quality of clinical practice.

AIM: To study the effect of sesamin on expression of inducible nitric oxide synthase(iNOS)and nitrotyrosine(NT)in rat liver tissue with metabolic syndromic hepatic steatosis. METHODS: The rat model of metabolic syndromic hepatic steatosis was induced by operation of two kidneys with one clip(2K1C)and high-fat. The rats taken from that successful model were randomly divided into model group and sesamin(120, 60, and 30 mg·kg~(-1)·d~(-1))groups. In addition, the sham-operated group was set up. The rats in treated group were given sesamin intragastrically everyday for 8 weeks. The levels of blood lipids(TC, TG and FFA)in serum were detected. The activity of SOD and MDA level in the liver homogenate were determined. The expressions of iNOS and NT proteins were detected by Western blotting analysis. The histopathological changes were observed by HE staining in the liver tissues. RESULTS: Compared to model groups, sesamin(120, 60 mg·kg~(-1))significantly inhibited the elevation of serum TC, TG, FFA, and MDA in liver homogenate(P<0.05), and increased the activity of SOD(P<0.05). It also decreased the protein expression of iNOS and NT(P<0.05), and ameliorated the degree of hepatic steatosis. CONCLUSION: Sesamin prevents and cures the metabolic syndromic hepatic steatosis. The mechanism is probably mediated through decreasing the protein expression of iNOS and NT, and alleviating the oxidative stress in addition to regulating the lipid metabolism.

Objective: To compare the therapeutic effects of penehyclidine hydrochloride on rat with oleic acid (OA)-induced acute lung injury (ALI) when given via inhalation and intravenous injection. Methods: Forty male Sprague-Dawley rats were randomly divided into 4 equal groups: normal control group(group I), ALI group(group II), penehyclidine hydrochloride inhalation group(group III), and intravenous penehyclidine hydrochloride group (group IV). The blood pressure (BP), pulmonary artery pressure (PAP), and airway pressure (Paw) were determined every 60 min during the experiment and the artery blood gas analysis and mixed venous blood gas analysis were performed. The wet to dry lung weight ratio (W/D) and the serum concentration of tumor necrosis factor alpha (TNF-α) were determined 240 min after the experiment in each group. Results: The OA-induced increase of PAP, Paw and decrease of PaO2/FiO2 were improved in the group III and group IV compared with those in the group II(P<0.05 for group III). The W/D and serum TNF-α concentration were lower in the group III and group IV compared with those in the group II (P<0.05 for group III). Conclusion: Penehyclidine hydrochloride can relieve the symptoms of OA-induced ALI. Inhalation approach is more effective than intravenous injection when the same dose of penehyclidine hydrochloride is given.

Adult ; Computer-Aided Design ; Humans ; Male ; Malocclusion ; diagnostic imaging ; therapy ; Orthodontic Appliance Design ; Orthodontic Brackets ; Orthodontic Wires ; Radiography, Panoramic

Child ; Humans ; Proteomics ; methods

Aim To investigate the difference of aortic function between renal hypertension rat(2K1C) and renal hypertensive-hyperlipidemia rat(RHHR).Methods Animals were divided into 3 groups: the sham-operated group,RHHR and 2K1C model groups.The vascular function test was performed in vitro.The response of aortic ring to phenylephrine(PE),acetylcholine(ACh) and sodium nitroprusside(SNP) was measured.Then aortic ring was incubated with nitricoxide synthase inhibitor N-nitro-L-arginine-methyl-ester(L-NAME) and its response to Ach was observed.Results The contraction in response to PE was augmented in the two model groups.ACh-induced vasorelaxation was reduced in both model groups,and such relaxation was more depressed in RHHR than that in 2K1C.The ability of relaxation evoked by NO was impaired in two model groups.SNP elicited complete relaxation in three groups,but the sensitivity to SNP was more decreased in RHHR than those in sham-operated and 2K1C groups.Conclusion These findings suggest that the vasodilation function of aorta is more impaired in RHHR than that in 2K1C group.The decrease in utilization of vascular smooth muscle to NO induced by hyperlipidemia may contribute to the main cause.

Objective To evaluate the effect of the high frequency oscillatory ventilation(HFOV) on the treatment of premature neonates with respiratory distress syndrome(RDS).Methods Seventy premature infants with RDS were randomly grouped as HFOV group(n=33) and conventional mandatory ventilation group(CMV group,n=37),based on their fetal age,weight,age,and their clinical condition from Jan.to Sep.in 2009.The blood gas analysis was detected and compared between the 2 groups.Results In HFOV group,the inhaled oxygen concentration,pa(CO2) decreased after treatment for 6 h,which were significantly lower than those at the beginning of the therapy(Pa