Objective: To observe the therapeutic effects of RFA combined with chemotherapy drug on hepatic VX: carcinoma in rabbits and to explore its mechanism.Methods: 40 rabbit models with VX: carcinoma were used for this study.they were divided into four groups at random.We watched the changes in serolog ALT,diameter of tumor and pathomorphology.And we also utilized terminal deoxynucleotidyl ransferase-mediated dUTP nick-end-labeling and immunohistochemistry to observe the apoptosis and proliferation.Results: ⑴The blood ALT significantly increased at the first day after treatment,then descended at the third day,and at the seventh day it descended to the level of pretreatment stage in group RFA combined with epirubicin.(2) The diameter of tumor showed significant difference between combined treatment and other groups.(3) Two weeks after treatment,the complete necrotic area slightly extended.it was clearly seen that the zone consisted of normal liver constitution,inflammation hyperemia zone and amorphous necrotic constitution by HE staining in combined treatment group.(4) The apoptosis index of the combined treatment group was bigger than that in other groups by TUNEL technology,but the PCNA index in the combined treatment group was lower than other groups.They all had significant difference.There was negative correlation between apoptosis index and PCNA index in the combined treatment group(r=-0.745,P

10 units allows identification of patients a high risk factors in development ARDS.The high risk fac- tors providing the greatest contribution to improve therapy in acute lung injury and prevent ARDS to occur.

Chemotherapeutic drugs conventionally used for their cytotoxic activity can also exert their influence on the immune system by grooming the tumor microenvironment,abrogating immune tolerance and inciting immune reconstitution.Due to their immunomodulatory features,chemotherapeutics,used as a preconditioning regimen and combined with subsequent immunotherapy,can mobilize the immune system to generate antitumor immune response.The synergy between chemotherapy and immunotherapy has brought some enlightenment to the development of new protocols for cancer treatment.

Objective To observe the application of alkaline cleaning agent in disinfection and supply room cleaning of medical devices, to evaluate the cleaning quality. Methods The 800 contaminated medical instruments collected from February 2016 to February 2017 in municipal maternal and child health hospital were divided into control group (February 2016 to August 2016) and study group (September 2016 to March 2017 ) according to different time periods,with 400 pieces in each group.In the reference group, the instrument was washed with multienzyme detergent, and the study group was cleaned with alkaline detergent. The cleaning of 2 groups of instruments was observed and compared. Results The total excellent and good rate of instrument cleaning in the study group was 97.25％ (389/400), in the reference group was 89.50％(358/400), the difference between the two groups was statistically significant (P<0.05); the cleaning safety, practicality, economy, comprehensive score difference of the study group was significantly better than the reference group (P<0.05). Conclusion The effect of alkaline cleaning agent used in disinfection supply room cleaning medical instruments is remarkable, and the equipment residue and microorganism can be effectively eliminated, and the utility model has the advantages of high safety and low economic cost, and has the important practical value.

Objective To investigate the antioxidation of bioactive proteins from the meat of Meretrix meretrix.Methods Three protein peaks(P1,P2 and P3) were isolated from the meat of Meretrix meretrix by homogenating,step precipitating with ammonium sulfate,chromatography with Sephadex G-150.CAT activity and SOD activity of these proteins were determined.Inhibition of the proteins to lipid peroxidation was determined.Results CAT activity of P2 was the highest,its specific activity was 77.0 U?mg-1.SOD activity of P3 was the highest,its specific activity was 68.8 U?mg-1.Inhibition of P3to lipid peroxidation was the most obvious,its inhibitive rate was 50% when the protein content was 20 ?g.SDS-PAGE results showed that the main composition of P1 was the protein,the Mr of which was 18.0 kD.The protein band of P2 corresponded with the protein band of contrast CAT,its Mr was 28.0 kD.The protein band of P3 corresponded with the protein band of contrast SOD,its relative Mr was 16.0 kD.Conclusion The bioactive proteins were isolated from the meat of Meretrix meretrix,and the bioactive proteins had antioxidation.

The paper reports the evaluation of the feasibility of using internal standard method for the determination of nicotine recovery in microdialysis in vitro. This in vitro experiment included two conditions. Nicotine and codeine phosphate were dissolved in Ringer's solution. Nicotine, codeine phosphate and the mixture of them were perfused through the CMA30 linear probe separately to calculate the proportion of the recovery (or delivery) of nicotine to that of codeine phosphate. And then codeine was perfused through the probe which was immersed in nicotine solution with different concentrations to calculate the proportion, too. In another condition nicotine was dissolved in rat plasma. The rat plasma protein binding rate was determined by using retrodialysis and internal standard method in vitro. The results are as follows: the proportion of the recovery (or delivery) of nicotine to that of codeine phosphate was fairly stable. The delivery of codeine was independent of nicotine concentration in the external medium. Protein binding rate determined by retrodialysis was almost the same as that determined by internal standard method. It suggests that the internal standard method is an effective way in the determination of nicotine recovery and codeine phosphate can be used as the internal standard.

Objective To explore the methods for the evaluation of the uncertainty of the determination of hardness in water. Methods A certain water sample was analyzed for the hardness according to EDTA-Na2 volumetry ruled by the Sanitory Standard for Drinking Water(2001).The mathematical model of the determination of hardness in water was established based on JJF1059-1999. The variance composition of the relative uncertainty was analyzed. The procedure of the evaluation of every uncertainty component was described in detail. Results The hardness in the water sample was 137 mg/L calculated according to mathematical model established in this study. The relative uncertainty and the expand uncertainty of the detection of hardness in the water sample were 0.008, 3 mg/L respectively. The expression of the uncertainty in the determination of hardness was (137?3)mg/L. Conclusion This method presented referable value for the evaluation of the uncertainty of volumetry determination method of hardness in water.

OBJECTIVE: To offer support for optimizing marketing strategy and increasing consumption in drug retailing.METHODS: Microsoft SQL Server 2005 database was established,the data mining method was subjected to a correlation analysis using association rules with the daily sales data in a retail drugstore in Jiangsu as an example.RESULTS & CONCLUSIONS: The data mining of drug retailing consists of 4 items: construction of drug sales data mart,data pretreatment,data mining,and analysis of mining results.The application of the data mining in retail drugstore can help analyze the purchase tendency of customers,find out potential customers and increase sales volume.

The "all-free health care" system is being implemented in Shenmu County in Shaanxi Province,whose ethical orientation is during the transitional process from "basic medical insurance" to "meeting residents′ actual healthcare demand".The "all-free health care" system safeguards human health rights,embodies the principle of giving priority to social benefits,and is in accordance with the principle of effectiveness.However,due to the lack of scientific management,the capital flows violate the principle of prevention in the system.