Objective To investigate the correlation between the plasm level of homocysteine(Hcy) and carotid intimal-medial thickness(IMT) in type 2 diabetes(T2DM).Methods 54 patients with preliminary diagnosis of T2DM were divided into two groups according to IMT level:normal IMT group and IMT increased group.30 healthy people were chosen as control.Hcy were detected in all patients and healthy people.Results Hcy levels in T2DM was (24.77 ± 10.17) μmol/L,which was higher than that in healthy people [(10.95 ± 2.60) μmol/L] (P ＜ 0.05).Hcy levels in IMT increased group was (19.80 ±4.97)μmol/L,which was higher than that in normal IMT group [(15.00 ±4.83) μmol/L] (P ＜ 0.05).The positive correlation between Hcy and IMT in T2DM was shown (r =0.6384,P ＜ 0.05).Conclusion High Hcy was an independent risk factor of atherosclerosis in patients with type 2 diabetes.

Objective To investige the diatolic/systolic oddi (DSO) of blood pressure and its relation to the gold section ratio (GSR)(0.618). Methods Blood pressures were measured and statistically analyzed in 604 patients with various types of hypertension. Dymamic changes of the blood presures were observed in patients treated with nitrobentin. Results The research on the aesthetic relationship between various types of blood pressure's DSO and GSR revealed that DSO of normal blood pressure approached the GSR, while that of the abnormal one deviated from GSR. Conclusion DSO may offer a new aesthetic parameter in assessing the human bodys aesthetics. This further verifies that the medical aesthetics of human body is natural collection of GSR.

Objective To evaluate the effect of the miracle questions in solution?focused brief therapy on memory tend and emotional stage in a non?clinical situation in college students. Methods A quasi?experimental design was adopted,and two kinds of materials for self?coaching( solution?focused,problem?focused) were devel?oped according to the previous researches. Seventy?two subjects were randomly divided into two groups,receiving the miracle questions in solution?focused brief therapy( The miracle question group,thirty?six subjects) or problem?focused coaching intervention(problem?focused group,thirty?six subjects).Exception memories number and positive and negative emotion level were evaluated between the two groups. Results The subjects receiving the miracle question group write more exception memories (3.67±2.07) than problem?focused group (1.61±1.18) ( t=5.178, P<0.01) . There was no significant difference in the positive and negative emotion level between the two groups(P>0.05) . Conclusion The miracle questions in solution?focused brief therapy can trigger the subjects to exceptional memories .This suggests that in practical application,questions order has impact on the effect of consultation. Be?fore asking exception question,miracle questions should be asked in the use of solution?focused intervention.

Allergens ; analysis ; Humans ; Hypersensitivity ; diagnosis ; immunology ; Practice Guidelines as Topic ; Skin Tests

Objective To investigate the distribution and effect of class Ⅰ integron in 40 strains of clinical multi-resistant Enterobacter cloacae. Methods Kirby-Bauer method was used to analyze the drug resistance of 40 strains of multi-resistant Enterobacter cloacae and the drug resistance gene was analyzed by polymerase chain reaction (PCR). Results 28 strains (70.0%) of 40 clinical isolates were observed to contain class Ⅰ integron. Drug resistance test showed their resistance rate to 10 kind of an-tibaeterials as follow: 7.5% to imipenem, 5.0% to meropenem, 75.0% to piperacillin-tazobactam,55.0% to amikacin, 12.5% to cefepime, 52.5% to ceftazidime, 55.0% to cefotaxime, 90.0% to ceftriaxone;81.8% to ticarcillin-clavutanic acid, and 85.0% to ciprofloxacin. Conclusion This study shows that the resistance situation of Enterobacter cloacae isolates is very serious, and class Ⅰ integron may play an important role in the muhidrug resistant mechanism.

Objective This study was aimed to evaluate the remote real-time transmission 12-lead electrocardiogram system on door-to-balloon time in patients with ST-segment elevation myocardial infarction.Methods We retrospectively analyzed the consecutive patients with STEM I who had accepted primary percutaneous coronary intervention (PCI) in the chest pain center of our hospital from February 2012 to July 2012.The study group consisted of patients with pre-hospital ECG,while the control group included patients without pre-hospital ECG,Their door-to-balloon time and door-to-catheter room time,mortality w ere compared.Results Totally 60 consecutive patients who had received primary PC I for STEMI were evaluated.Among them,35 patients were hospitalized with pre-hospital ECG while the other 25 patients without ECG.The Pre-hospital ECG was associated with a significautly shorter median door-to-balloon time (38 min vs.94min;P ＜0.01),The proportion of patients received balloon dilation within the guidelinerecommended 90 min timeframes Was significantly higher in pre-hospital ECG group than in non pre-hospital ECG group (94.6％ vs.60％;P =0.001).No difference was observed in mortality between the two groups (5.7％vs.4％;P ＞ 0.05),Significant difference was seen in the median hospital time in study group (5 compared with control group (7day) (5 day vs.7 day;P ＜ 0.01).Conclusions The remote real-time transmission 12-lead electrocardiogram system is associated with a significantly shorter door-to-balloon time in STEMI patients.The remote real-time transmission 12-lead electrocardiogram system is recommended in patients suspected STEMI.

Objective To investigate temporal expression of estrogen receptor (ER) in myocardium at non-infarct zone after acute myocardial infarction (AMI) in rats. Methods 120 healthy, male SD rats were randomly divided into three groups: control group, left anterior descending coronary artery (LAD) occlusion group and sham operation group with 8 rats in each group. The murine AMI-model was established by LAD ligation after anesthetization, and the rats in both LAD occlusion group and sham operation group were sacrificed at 2h, 4h, 8h, 12h, 1d, 2d, 4d, and 9d after occlusion. Myocardial samples were collected. H.E and ER immunohistochemical staining were performed. The results were quantitatively evaluated by image analysis system. The data were statistically analyzed with SPSS for Windows. Results ER expression in non-ischemic cardiomyocytes after LAD occlusion became more prominent with extension of LAD occlusion period. There was no significant difference within 12h after AMI, while ER expression was enhanced significantly 24h after ischemia. The enhancement were more evident from 4 to 9 days. Conclusion The results suggested that protective role may be initiated in response to acute ischemia in cardiac cells at non-infarct zone by increased ER expression after LAD occlusion in rats.

Objective To investigate the application value of spiral CT imaging in diagnosing costal cartilage diseases. Methods CT volume scan with 1～7mm layer thinkness and 1～2.5mm layer distance was performed in 28 cases of costal cartilage diseases and 200 cases of controls. All of the original images were processed to form the thin layer reconstruction of low contrast and high contrast, and then the reconstructed images were transmitted to CT 3D work-station to perform the image reconstruction by MPR, MIP and SSD methods. The ability of different image techniques in displaying costal cartilage was compared, and costal cartilage pathological changes were analyzed. Results The CT scanning images of 228 cases could display the costal cartilage after the management of MPR, MIP and SSD, and the images managed with MIP and SSD were better. Among 28 patients with costal cartilage diseases, 13 cases of costal cartilage damage,9 cases of costal cartilage inflammation and 6 cases of costal cartilage malformation were found. Conclusion Spiral CT image could display the costal cartilage perfectly, was a new means of researching costal cartilage morphology in living body, and was the best imaging technique of researching costal cartilage disease without trauma.

0.05). The data proved that PSMA7 was overexpressed in pcDNA3.1(-)/PSMA7-transfected A549 cell line, both in mRNA level and in protein level. Conclusion Eukaryotic expression plasmid pcDNA3.1(-)/PSMA7 has been successfully constructed, and the PSMA7 is stably expressed in A549 cell line. This would pave the way for further study of PSMA7.

Aim To investigate the effect of oral itraconazole on pharmacokinetics of viaminate and under-stand the metabolism pathway of viaminate in healthy humans,as well as guide the reasonable medication for viaminate and itraconazole coadministration.Methods The study was conducted in a double-blind randomized and crossover manner with two phases of treatment with placebo-viaminate or itraconazole-viaminate.Eight healthy male subjects received an oral dose of 200 mg itraconazole(QD) or matched placebo for five consecutive days,and took an oral 100 mg dose of viaminate on day 4 after each treatment phase.The liquid chromatography-mass spectrometry(LC-MS) was established to measure the blood drug concentration of viaminate.Results Our results showed that the mean peak concentration of viaminate was increased with itraconazole [(21.21?11.65) ?g?L~(-1) vs(27.12?13.83) ?g?L~(-1),P0.05].Additionally,the mean elimination half life was prolonged [(2.71?0.38) h vs(4.43?0.93) h,P