Serum sialy transferase(ST) and fucosyltransferase(FT) were determinated in 56 patients with nasopharyngeal or laryngeal carcinoma. 18 healthy were normal control. Serum sialic acid and fucose were also determinated in each sample.Comparison were made statistically among groups of stages of cancer, having metastases or no metastases, before treatment,and recurrence or non-recuurrence after cure Our results suggest that determinations of serum ST, FT, sialic acid and fucose could be used as new-indicators of diagnosis f.or advanced nasopharyngeal or laryngeal cancer and differentiation between recurrence and non-recurrence after treatment. However,the levels of ST in serum could be evaluated for metastases or non-metastases before treatment

Objective To research the effect of Hydroxyl radical(type Ⅰ mechanism)and singlet oxygen(type Ⅱ mechanism)on virus inactivation and red cell cytotoxicity induced by DMMB and M007 phototreatment.Methods Hydroxyl radical quenchers mannitol and glycerol,singlet oxygen quenchers sodium azide and tryptophan were employed to investigated their ability to inhibit the inactivation of bacteriophage Phi6 and red cell cytotoxicity by induced DMMB and M007 phototreatment.Results Singlet oxygen quenchers could inhibit the inactivation of bacteriophage Phi6 obviously, whereas hydroxyl quenchers hardly affect the inactivation of bacteriophage Phi6.Both type of quenchers could reduce the hemolysis caused by CMMB or M007 phototreatment.Conclusion Viral inactivation of DMMB and M007 phototreatment is mainly through type Ⅱ mechanism, both type I and type Ⅱ mechanisms contribute to red cell cytotoxicity.

Objective:To test the accuracy of Tri Auto ZX electronic apex locator(Morita,Japan)and JustyⅡ electronic canal length meter(TME-601,Yoshida Toei Engineering Company,Japan).Methods:26 root canals were tested in vitro.Electronic canal length(L_E) measurements were recorded with Tri Auto ZX and Justy Ⅱ respectively at the reading "APEX" and "0.5".Under stereoscope the deviation of L_E from the actual working length and degree of accuracy were determined.Results:The average deviation of the measurements obtained by Tri Auto ZX was significantly lower than that by JustyⅡat reading"0.5"(P

0.05). Conclusion: The PGI 2 level in dental pulp tissue is closely related to the degree of pulp pain.

Objective To study the behavior factors associated with nonalcoholic fatty liver disease (NAFLD).Methods A total of 2038 subjects who underwent physical examination at the Department of Health check-up center,the Affiliated Hospital of Medical College,Qingdao University were included in this study(including 1438 cases of NAFLD).A questionnaire survey was conducted on their lifestyle and behaviors.Data of routine physical examination,ultrasound examination,liver function and blood lipid test were collected and analyzed.Results Multi-factors Logistic regression analysis showed that age (20 to 49 years) male sex,stress from work,sleep time, physical activity and diet axe among factors associated with NAFLD(all P＜0.05).Conclusion Lifestyle and behavior factor are associated with the incidence of NAFLD.

9.9Log10 bacteriophage R 17 Conclusion Alkylation of the phenothiazine ring increased the potential of inactivating virus and decreased the damage to red blood cells? M007 might be an alternative phenothiazine dye that can be used to inactivate viruses in red blood cells?

Objective: To develop Epstein-Barr virus(EBV)-transformed human peripheral blood B cell lines from healthy volunteers of type B.Methods: B lymphocytes from healthy volunteers of type B capable of producing anti-A antibodies were transformed by EBV,while Cyclosporine A(CsA),as an immunosuppressive agent that could selectively inhibit T-lymphocytes and protect B-lymphocytes from regression,was used in the experiment.Then,the supernanant of the cell culture medium was tested with red blood cells of type A,B and O by agglutinin assay.Results: Twelve of the15 EBV-transformed B lymphocyte cell lines were established,with a success rate of 80%,while 9 human B lymphocyte lines secreted anti-A antibodies.Conclusion: Human B lymphocyte lines secreting antibodies to A antigens were successfully developed,which helps further studies of human blood specific monoclonal antibodies.

Objective: To construct a phage display library of human single-chain Fv antibodies against blood group Rh(D) substance. Methods: Combining phage display library techniques, isolated total RNA from B lymphoblastoid cell lines secreting anti-Rh(D) antibodies was used for the synthesis of the first strand of cDNA, V_ H and V_ L genes were amplified by 2nd PCR and linked together by splicing overlap extension (SOE) with the use of a (Gly_ 4Ser)_ 3 linker. The resulted scFv genes were then cloned into pCANTAB5E vectors and displayed on the phage. Phage clones were selected using intact red cells as a source of antigen. After 4 rounds of "binding-elution-enrichment", each clone was assayed for specificity by Dot ELISA. Results: A phage antibody library, with the sink size being 1.2?107, was obtained. The percentage of full-length scFv gene inserted into phage DNA was 0.80. Rescued by helper phage, a phage scFv library with titer of 3?108 pfu/ml was established. Specific phages with scFv were acquired after 4 rounds of panning, one clone exhibiting specific binding to Rh+ cell was identified by Dot ELISA. Conclusion: A strategy for construction phage antibody library by means of phage display technique was practicable, which would be useful in screening engineered antibodies against human Rh (D) blood group substances.

Recently,thesituationofchemicalsubstancesillegallyaddedinTCMandhealthproductsismoreandmorecomplex and subtle, which not only results in latent danger and harm to customers, but also induce supervision difficulty and challenge for su-pervision departments. The variety and composition of chemical substances added illegally, analytical methods and relative state laws and regulations were illustrated in detail in the paper, which can provide the basis for the rational and safe use of TCM and health prod-ucts, and provide the reference and technology support for the supervision departments.

Objective To investigate the ultrasound diagnostic value for nipple discharge disease by comparison with fiberoptic ductoscopy. Methods Fiberoptic ductoscopy and ultrasonic inspection were performed on fifty-seven patients with spontaneous nipple discharge,and the results were analysed. Results In all patients, 55 cases (96. 49 % ) were abnormal in high-frequency ultrasound inspection:27 mammary duct expansion(47.36%),19 occupying lesion with mammary duct expansion or cyst (33.33%),6 cystic echo ( 10. 52% ) ,3 only solid echopattern (5.26%) ,and 2 nothing found (3.51% ). In ultrasonic inspection,color and pulse Doppler signal were found in 7 cases. On the other hand, 26 galactophoritis (45.61%), 12mammary duct ectasia (21.05 %), 17 intraductal papilloma (29.82%), 2 breast carcinoma (3.50%) were diagnosed by fiberoptic ductoscopy. In all 19 cases with occupying lesion,ultrasonography's sensitivity was 52. 63% ,specificity was 89. 47%, misdiagnosis rate was 10. 50%, rate of missed diagnosis was 47.36%.Conclusions To nipple discharge disease, high-frequency ultrasound has high sensitivity, easy to find abnormal indication,but is not accurate enough in the diagnosis of smaller occupying lesion.