Objective:To probe the mechanism of Dachengqi Decoction combined with the treatment principle of promoting production of body fluid with sweet-flavored and cold-natured drugs on the earth-dry and water-exhaust syndrome.Methods:Chinchilla Giganta rabbits were randomly divided into four groups,i.e. blank control group,pathological model group,timely purgation to preserve Yin treatment group and treatment group of purgation with drugs sweet in favor and cold in nature.Changes of symptoms,signs,pathological changes of organs,WBC count,nitric oxide(NO),superoxide dismutase(SOD),malondialdehyde(MDA),middle molecular substances(MMS),sulfydryl(-SH),K+ and Na+,and viscosities of whole blood and plasma before and after treatment were investigated.Results:There were significant differences in decrease of body temperature,increases of SOD,MMS,K+ and Na+,decreases of NO,MDA,-SH and viscosities of whole blood and plasma as the two treatment groups compared with the pathological model group(P

Objective To investigate the clinical importance and the relationship between the levels of plasma total homocysteine (Hcy), serum folic acid and vitamin B 12 , and the occurrence and development of ischemic leukoaraeosis (LA). Methods Ischemic LA group and normal control group with 100 cases for each were studied. The 100 cases in ischemic LA group were firmly diagnosed by CT or / and MRI. The levels of plasma total Hcy, serum folic acid and vitamin B 12 of the patients in ischemic LA group were measured, and compared with that in control group. Results The level of plasma Hcy in ischemic LA group (19.79?5.25?mol/L) was significantly higher than that in control group (12.02?2.11?mol/L), while the levels of serum folic acid and vitamin B 12 in ischemic LA group (2.08?0.84ng/ml and 140.13?45.49pg/ml) were lower than that in control group (4.39?1.30ng/ml, 297.45?120.23pg/ml). The differences between two groups were statistically significant (P

Objective Preparation of specific antibody of neuron protein 14 3 3 and application for the diagnosis of prion diseases. Methods The rabbits were immunized with prokaryotic expressed GST 14 3 3 fusion protein and the collected antiserum was evaluated by ELISA and Western blot assays. Results ELISA revealed that the reactive titer of the prepared antibody against purified 14 3 3 protein was up to 1:4 096 000; Western blot assays confirmed that both prokaryotic expressed,and the natively presenting 14 3 3 proteins in the brain extracts were recognized by the prepared antibody. Two cases of clinically diagnosed Creutzfeldt Jakob disease (CJD)with typical EEG changes showed the positive 14 3 3 protein in cerebral spinal fluid (CSF). Four patients with dementia and narcoma but without typical EEG change were 14 3 3 negative in CSF,three of them being even further tested by brain biopsies without typical histological change of CJD and presentation of protease resistant PrP Sc protein. 35 samples CFS collected from patients without dementia were 14 3 3 negative in CSF. All the results were the same as that of standard 14 3 3 polyclonal antibody.Conclusion Using purified prokaryotic expressed GST 14 3 3 fusion protein as antigen,the specific antibody was elicited in the immunized animals. The prepared antibody can be used in identification of 14 3 3 protein in CSF for the diagnosis of CJD.

Objective To compare the application value and the clinical curative effect of longitudinal incision and tarsal sinus minimal-ly invasive approach in treatment of patients with intra-articular calcaneal fractures. Methods A retrospective statistical analysis was made by collecting and comparing the clinical data of 67 patients with intra-articular calcaneal fractures from March 2008 to March 2012,and they were divided into the longitudinal incision minimally invasive approach group ( ZW group,36 patients,37 feet) and the tarsal sinus minimally invasive approach group ( FW group,31 patients,33 feet) . The Bohler angle, Gissane angle before and after operation,complications healing time,and AOFAS scores were compared. Results The healing time,Bohler angle,Gissane angle of ZW group and other indicators were better than those of the FW group,but there was no significant difference between the two groups (P>0. 05). The infection of incision and compli-cations in ZW group was obviously less than those of the FW group (P<0. 05). According to the AOFAS scoring system,ZW group scored 82. 49 points while FW group scored 86. 53 points,which indicated no statistical significance (P>0. 05). Conclusion The two kinds of treatments are of no obvious difference,but the longitudinal small incision minimally invasive approach could receive better effect in terms of soft tissue damage,healing time,and postoperative complications.

ObjectiveTo assess different treatments on nonspecific low back pain.Methods246 subjects were assigned to 3 groups, a control group(n=82), a home exercise group(n=82) or a strengthening exercise group(n=82). Follow-up data were obtained at 1 months, 3 months and 6 months after the treatment. Primary outcome measures were modified Oswestry Low Back Pain Disability Questionnaire for disability and Numeric Pain Rating Scale for pain.ResultsThe home exercise and strengthening exercise groups experienced greater improvements in disability than the control group 6 months after the treatment. The strengthening exercise group demonstrated greater change than home exercise group.ConclusionActive rehabilitation therapy may benefit nonspecific low back pain patients.

Objective: To analyze the surveillance results of common diseases among primary and middle school students in Xinjiang Production and Construction Corps in 2020, so as to provide the evidence for formulating the control measures for common diseases among primary and middle school students. Methods: The comprehensive health surveillance data of 31 467 primary and middle school students were collected from 132 schools in 14 divisions of Xinjiang Production and Construction Corps in 2020, and the prevalence of poor vision, overweight and obesity, dental caries, malnutrition and elevated blood pressure were descriptively analyzed. Results: The 31 467 students included 15 580 boys ( 49.51% ) and 15 887 girls ( 50.49% ). There were 19 632 students ( 62.39% ) from urban areas and 11 835 ( 37.61% ) from suburban areas, and there were 11 371 primary students ( 36.14% ), 11 169 junior high school students ( 35.49% ) and 8 927 high school students ( 28.37% ). The detection rates of poor vision, overweight and obesity, dental caries, elevated blood pressure, and malnutrition were 57.22%, 29.69%, 27.57%, 15.03%, and 4.81%, respectively. The detection rates of overweight and obesity ( 36.07% vs. 23.45%, P<0.05 ) and malnutrition ( 5.71% vs. 3.93%, P<0.05 ) were significantly higher, while the detection rates of poor vision ( 51.61% vs. 62.72%, P<0.05 ), dental caries ( 24.46% vs. 30.63%, P<0.05 ) and elevated blood pressure ( 14.61% vs. 15.43%, P<0.05 ) were significantly lower in boys than in girls. The detection rates of overweight and obesity ( 30.17% vs. 28.91%, P<0.05 ) and poor vision ( 61.55% vs. 50.04%, P<0.05 ) were significantly higher, while the prevalence rates of malnutrition ( 4.55% vs. 5.24%, P<0.05 ), dental caries ( 24.47% vs. 32.73%, P<0.05 ) and elevated blood pressure (14.66% vs. 15.63%, P<0.05) were significantly lower in students from urban areas than from suburban areas. In addition, the prevalence of malnutrition and poor vision appeared a tendency towards a rise, while the prevalence of overweight and obesity, dental caries and elevated blood pressure appeared a tendency towards a decline among primary and middle school students with the study period (P<0.05 ). Conclusions The prevalence of poor vision, overweight and obesity, and dental caries is relatively high among primary and middle school students in Xinjiang Production and Construction Corps, and comorbid overweight and obesity and malnutrition is found.

OBJECTIVEThis study was performed to determine the feasibility of digital virtual positioning of braces in clinical applications.

METHODSWe determined the positions of brackets in 28 teeth of 15 cases according to positioning methods that use bracket height or root information. Final status was generated by OrthoRx software. Treatment results were assessed with American board of orthodontics objective grading system (ABO-OGS).

RESULTSThe deduction points in the marginal ridge height were significantly decreased after treatment using the two methods (P<0.05). However, no statistically significant difference was found in the deduction points in the marginal ridge height between the two methods (P>0.05). No improvement was found in the deduction points in root parallelism with both methods. No statistical significance was found in the deduction points in root parallelism before and after treatments using both methods. The deduction points in alignment and the three scoring components were significantly decreased after treatment using the two positioning methods. In addition, statistically significant differences were found in the deduction points in alignment between two methods (P<0.05).

CONCLUSIONBy using OrthoRx software, we achieved effective therapeutic treatment in reconstructing three-dimensional digital modes using two different bracket-positioning methods. The positioning method that used root information is more accurate compared with the bracket height positioning method. This study provides an experimental basis for bracket accuracy in the mouth.

The immunoregulatory effect of thalidomide on peripheral blood mononuclear cells in patients with thyroid-associated ophthalmopathy (TAO) was investigated.The resuhs showed that thalidomide (50 μg/ml)inhibited the proliferation of PBMC significantly.Thalidomide inhibitied tumor necrosis factor-α,IFN-γ,and interleukin-6 secretion,as well as mRNA expressions.

Objective To investigate the role of endoscopic retrograde cholangiopancreatography (ERCP) management in the diagnosis and treatment and illustrate clinical features of non-iatrogenic hemobi lia.Methods A total of 35 patients diagnosed as hemobilia via endoscope from August 2009 to September 2012 were reviewed retrospectively.Patients with iatrogenic causes of hemobilia were excluded in this study.Results The clinical features of hemobilia mainly included jaundice (77.1％),abdominal pain (62.9％),hematemesis (5.7％),melena (2.9％) and hematochezia (2.9％).Quincke triad was found in 4 patients with hemobilia.Hemobilia were caused by hepatobiliary malignancies in 29 patients and benign biliary tract diseases in the other six.Biliary obstruction caused by hemobilia was successfully ameliorated by ERCP in 34 patients.Postoperative pancreatitis ocurred in 1 patient.Conclusion The common noniatrogenic causes of hemobilia were hepatobiliary and pancreatic malignancies.ERCP is recommended as the initial management to confirm the hemobilia and solve biliary obstruction.

Objective To investigate the relationship between quantitative detection of T lymphoma invasion and metastasis inducing factor (Tiaml) mRNA and invasion,metastasis and prognosis of patients with colorectal cancer.Methods The quantitative expression of Tiam1 mRNA in cancer tissues in colorectal cancers (n =97),in tissues of colorectal adenomatous polyposis (n =49) and normal control (n =30) were detected with real-time fluorescence quantitative-polymerase chain reaction (PCR).The relationship between the quantitative expression of Tiam1 mRNA in colorectal cancer tissues and the invasion,metastasis and the prognosis were analyzed.Results The levels of Tiam1 mRNA expression in the colorectal cancer group,were significantly higher than those in colorectal adenomatous polyposis group and normal controlgroup [(14.45±4.87)2-△△△Ct vs (3.77 ±3.15)2-△△Ct,(1.06 ±0.65)2-△△Ct,P ＜0.01].The levels of Tiam1 mRNA expression in the adenomatous polyposis with high grade intraepithelial neoplasia were signifiCantly higher than those in the adenomatous polyposis with low grade intraepithelial neoplasia [(5.26 ±2.45)2-△△Ct vs (3.78 ±2.19)2-△△Ct,P ＜0.01],which were both significantly higher than those in the adenomatous polyposis with no intraepithelial neoplasia [(2.13 ± 1.64)2-△△Ct,P ＜0.05 or P ＜ 0.01].The levels of Tiam1 mRNA expression in colorectal cancer group were positively related to the differentiated degree of tumor,depth of invasion,lymph node metastasis,tumor node metastasis (TNM) stages and the postoperative recurrence (P ＜ 0.01),and were negatively related to the postoperative survival time (P ＜0.01).Multivariate Cox regression analysis revealed that levels of Tiam1 mRNA expression in cancer tissues were independent prognostic factor for colorectal cancer (Exp =4.043).Conclusions The high Tiam1 mRNA expression levels in cancer tissues are closely related to invasion,metastasis and poor prognosis of colorectal cancer,and may be a prognostic indicator of colorectal cancer.The increase expression of Tiam1 mRNA in colorectal adenomatous polyposis may be related to colorectal tumorigenesis.