BACKGROUND:Vascular endothelial injury usually appears following atherosclerosis, hypertension, diabetes mellitus and other diseases, which can be repaired by endothelial progenitor cells (EPCs) in the systemic circulation. OBJECTIVE:To study the effect of salveanolic acid B on the mobilization and adhesion of EPCs in mice. METHODS:Thirty C57BL/6 mice were selected and randomly divided into surgical and non-surgical groups, (n=15 per group). Common carotid arteries of mice in the surgical group were injured by iron wire, then were subdivided into three groups (n=5 per group), and were given the injection of 5% of glucose (control group), 100 g/L salveanolic acid B and 10 μmol/L vascular endothelial growth factor, respectively. Meanwhile, the mice in the non-surgical group received the same assignment and treatment, but the common carotid arteries were not injured. After consecutive 7-day administration, the EPCs in the peripheral blood of the mice were isolated, and the EPCs adhesion was tested by flow chamber experiment. The serum levels of stromal cell-derived factor-1 and interleukin-8 were detected by ELISA kits. RESULTS AND CONCLUSION:The proportion of EPCs in the surgical group was significantly increased compared with the non-surgical group (P < 0.05). After 7-day administration, the proportion of EPCs in the salveanolic acid B and vascular endothelial growth factor subgroups of the surgical group was significantly higher than that in the control subgroup (P < 0.05); the proportion of EPCs in the vascular endothelial growth factor subgroup of the non-surgical group was significantly higher than that in the control and salveanolic acid B subgroups (P < 0.05). Flow chamber experiment revealed that the number of EPCs in the salveanolic acid B subgroup was significantly more than that in the control subgroup (P < 0.05). In the surgical group, the serum levels of stromal cell-derived factor-1 and interleukin-8 in the in the salveanolic acid B and vascular endothelial growth factor subgroups were significantly higher than those in the control subgroup (P < 0.05); in the non-surgical group, the serum levels of stromal cell-derived factor-1 and interleukin-8 in the in the vascular endothelial growth factor subgroup were significantly higher than those in the control subgroup (P < 0.05). To conclude, salveanolic acid B can increase the efficiency of the EPCs mobilization in bone marrowin vivo, help the EPCs adhere to the collagen surface, and upregulate the serum levels of stromal cell-derived factor-1 and interleukin-8.

ObjectiveTo explore the relationship between rotary strength of trunks and stability of the low back in patients with nonspecific low back pain. MethodsBefore sling exercise therapy (SET), 22 patients with chronic nonspecific low back pain were measured the rotary strength of core muscles of trunks with Proxomed Tergumed functional spinal evaluation and training system and the core stability of the low back with SET system. Their correlation was analyzed. ResultsThe core rotary strength was positively correlated with the local stability in patients with chronic nonspecific low back pain (r=0.876, P<0.001). ConclusionIncreasing the rotary strength of trunks is important to improve local stability in patients with chronic nonspecific low back pain.

AIM: To study the DNA fingerprinting of crude and processed Atractylodes lancea. METHODS: DNA fingerprinting of samples of crude and processed Atractylodes lancea were examined by RAPD. RESULTS: All crude Atractylodes lancea showed similar fingerprinting characteristics,while the processed Atractylodes lancea products indicated considerable different results. CONCLUSION: DNA fingerprinting is a reliable method for di-(fferentiating) crude Atractylodes lancea.

Objective To compare the application value and the clinical curative effect of longitudinal incision and tarsal sinus minimal-ly invasive approach in treatment of patients with intra-articular calcaneal fractures. Methods A retrospective statistical analysis was made by collecting and comparing the clinical data of 67 patients with intra-articular calcaneal fractures from March 2008 to March 2012,and they were divided into the longitudinal incision minimally invasive approach group ( ZW group,36 patients,37 feet) and the tarsal sinus minimally invasive approach group ( FW group,31 patients,33 feet) . The Bohler angle, Gissane angle before and after operation,complications healing time,and AOFAS scores were compared. Results The healing time,Bohler angle,Gissane angle of ZW group and other indicators were better than those of the FW group,but there was no significant difference between the two groups (P>0. 05). The infection of incision and compli-cations in ZW group was obviously less than those of the FW group (P<0. 05). According to the AOFAS scoring system,ZW group scored 82. 49 points while FW group scored 86. 53 points,which indicated no statistical significance (P>0. 05). Conclusion The two kinds of treatments are of no obvious difference,but the longitudinal small incision minimally invasive approach could receive better effect in terms of soft tissue damage,healing time,and postoperative complications.

Objective To explore effects of sling exercise therapy on back pain of adults with idiopathic scoliosis.Methods38 subjects were age-matched assigned to a control program (n=18) or a treatment program (n=20). Follow-up data were obtained 4 weeks after treatment. Primary outcome measures were disability (Oswestry Low Back Pain Disability Questionnaire) and pain (Numeric Pain Rating Scale).ResultsThe treatment group experienced greater improvements in disability and pain compared with the control program 4 weeks after treatment.ConclusionSling exercise therapy may benefit back pain of adults with idiopathic scoliosis.

Plantar pressure distribution can reflect the force of several key points on foot while standing and walking. A comprehensive understanding of the plantar pressure distribution makes great sense in the following aspects: the understanding of the normal foot biomechanics and function, clinical diagnosis, measurement of disease extent, postoperative efficacy evaluation, and rehabilitation research. A simple plantar pressure measurement device was designed in this study. This paper uses FlexiForce flexible sensor to pickup plantar pressure signal and USB A/D board to do data acquisition. The data are transferred into a laptop and processed by a VB-based software which can display, remember and replay the data. We chose patients with hallux valgus and normal people to measure the pressure distribution and make contrast analysis of plantar pressure with this device. It can be concluded that people with hallux valgus have higher pressure on the second metatarsophalangeal joint and the distribution move outward. The plantar pressure of patients postoperative could be greatly improved compared to the preoperative. The function of this device has been confirmed.
Foot ; physiology ; Hallux Valgus ; physiopathology ; Humans ; Metatarsophalangeal Joint ; physiopathology ; Monitoring, Physiologic ; instrumentation ; Pressure ; Walking

Objective To evaluate the clinical effect of umbilical cord mesenchymal stem cells (MSCs) for treatment of chronic dilated cardiomyopathy complicated with systolic heart failure.Methods Fifty-nine patients with chronic dilated cardiomyopathy and systolic heart failure in Henan Provincial People's Hospital from December 2013 and December 2014 were selected and divided into treatment group (n =30)and control group (n =29).All patients were given routine drug treatment.Based on this,the patients in treatment group were given umbilical cord MSCs 20mL by intracoronary transplantation;the patients in control group were given the same volume of saline.The changes of cardiac function,left ventricular ejection fraction (LVEF),left ventricular end diastolic diameter(LVEDD),6 minutes walking distance,the amino-terminal pro-brain natriuretic peptide (NT-proBNP) levels,the re-hospitalization rate,mortality rate of patients in the two groups were observed and compared at 1 month and 6 months after treatment.Results The cardiac function grading of the New York Heart Association (NYHA)of patients in the two groups were improved at 1 month and 6 months after treatment (P ＜ 0.05);the NYHA cardiac function grading of patients in the treatment group was significantly better than that in the control group at 1 month and 6 months after treatment (x2 =12.64,16.75;P ＜ 0.05).There was no statistic difference in LVEDD,LVEF,serum level of NT-proBNP and 6-min walking distance of patients between the two groups before treatment (P ＞ 0.05).There was statistic difference in LVEDD,LVEF,serum level of NT-proBNP and 6-min walking distance of patients in the two groups before treatment and 1 month,6 months after treatment(P ＞ 0.05).There was no statistic difference in LVEDD of patients between the two groups at 1 month after treatment (P ＞ 0.05);the LVEF,6-min walking distance of patients in the treatment group were significantly higher than those in the control group,but the serum level of NT-proBNP of patients in the treatment group was significantly lower than that in the control group at 1 month after treatment (P ＜ 0.05).At 6 months after treatment,the LVEDD,serum level of NT-proBNP of patients in the treatment group were significantly lower than those in the control group,but the LVEF and 6-min walking distance of patients in the treatment group were significantly higher than those in the control group (P ＜ 0.05).The mortality rate of patients in the treatment group (6.67％,2/30) was significantly lower than that in the control group (24.14％,7/29) (x2 =4.99,P ＜ 0.05).The re-hospitalization rate of patients in the treatment group and control group was 16.67％ (5/30) and 31.031.(9/29) respectively;there was no statistic difference in the re-hospitalization rate of patients between the two groups (x2 =1.68,P ＞ 0.05).Conclusion Umbilical cord MSCs can improve the cardiac function and cardiac remodeling,reduce the mortality rate of patients with chronic dilated cardiomyopathy complicated with systolic heart failure.

Objective To understand the situation of college students' awareness about innovative credit, analyze the influencing factors, in order to provide a scientific basis for constructing innovation credits and promoting the double strategy. Method The undergraduates of a medical university who were still at school in April 2015 were selected, and by using a stratified cluster sampling method, 450 students were chosen to be conducted by self-administered questionnaire survey to understand the cognitive status of in-novation of credit system in college students. Chi square test was used to compare the passing rates of students in different demographic characteristics, and the relationship between scores was analyzed by linear regression analysis. Result A total of 439 valid questionnaires were collected , and the total scores of innovative knowledge, attitudes and behaviors of 439 undergraduates were 15.95％ (70/439), 36.44％ (160/439) and 32.80％(144/439) respectively. In the part of knowledge innovation credits, girls' passing rate was higher than boys ( χ2=4.010,P=0.045. In the part of attitude, high grade group students had more positive attitude than the low grade group of students ( χ2=6.227,P=0.0013). In the part of behavior; higher grade students with innovative credits had higher pass rates than those in lower grades (chi, 2=7.781, P=0.005), and boys had a higher rate of passing than girls ( χ2=6.658,P=0.010). The total score of knowledge was positively linear with the total score of attitude and behavior. Conclusion College Students' awareness of innovation credit rate is low, but the innovative attitude and behavior is positive. The higher the awareness rate is conducive to the cultivation of attitude and behavior, so it is necessary in medical colleges to carry out innovation of credit and promote the cultivation of innovative consciousness.

Objective To evaluate the clinical efficacy of dendritic cells-cytokine induced killer cells combined with surgical treatment for primary liver cancer.Methods Totally 78 patients with primary liver cancer were randomly divided into experiment group (n =30) and control group (n =48).The patients in experiment group received hcpatectomy combined with dendritic cells-cytokine induced killer cell treatment while those in control group were given hepatectomy treatment.The median time to recurrence,progression-free survival,survival and quality of life were evaluated.Observed side effects of cell therapy in experiment group.Results Experiment group received a total of dendritic cells-cytokine induced killer cell treatment 78,an average of 2.6 times per person.Fever occured in 8 patients who received dendritic cells-cytokine induced killer cell treatment.After one cycle of immune therapy,the KPS score of 20 cases was improved,8 cases were stable and and 2 case was worsen in the experiment group.The KPS score of 10 cases were improved,32 cases were stable and and 6 cases were worsen in the control group,and the difference is statistically significant (P ＜ 0.05).The progression-free survival rates for 1,2 and 3 years in the experiment group were 73.3％,40.0％,23.3％ and 68.7％,27.0％,14.5％ in the control groups,respectively.The progression-free survival rates in the experiment group were improved compared to the control group and the difference is statistically significant (P ＜ 0.05).The median time of recurrence in the experiment group were (16.9 ± 2.6) months and (13.5 ± 2.9) months in the control group,respectively (P ＜ 0.05).The 1-2-3-years survival rates in the experiment group were 85.0％,50.0％,35.0％ and 85.0％,40.0％,23.3％ in the control group respectively.There was no statistically significant difference between these two groups (P ＞ 0.05).Conclusions Dendritic cellscytokine induced killer cells combined with surgical treatment on primary liver cancer is safe and effective,it can improve quality of life,and delay the recurrence time after surgery.But not improve long-term survival.

Objective To investigate the clinical effect and safety of spleen-preserving surgery by microwave tissue coagulation (MTC) therapy.Methods Retrospectively analyzed the clinical data of 45 cases undergoing spleen retaining surgery by MTC therapy (observation group) and comparative study was used on another 45 cases experiencing splenectomy (comparative group),clinical effect and complications were compared.All cases were patients from Jan.2010 to Jun.2013.Results All cases were cured.Hospitalization of observation group is obviously shorter than that of comparative group(P =0.007).The rate of complication in observation group(4.44％) is lower than that in comparative group (20.00％),but the time and amount of bleeding in operation of observation group is much more than that of comparative group.Conclusion MTC can effectively guarantee patients safety,shorten hospital stay,and be worthy of popularization.