Objective To explore the effect of dexmedetomidine on the stress reaction and incidence of shivering in intravenous inhalation combined anesthesia scheme,and to improve the quality of anesthesia recovery accumulate experience.Methods 76 patients in Affiliated Union Hospital of Fujian Medical University from 2010 March to 2013 April were divided into study group and control group using random double blind method(n=38).Two groups of patients were underwent intravenous inhalation combined anesthesia,the research group in the process of anesthesia administered dexmedetomidine intravenous infusion,the control group was given normal saline intravenous infusion of the same dose,the same rate.The recovery period MAAS (muscle activity score),HR (heart rate),MAP (mean arterial pressure)and the incidence of shivering of two groups were observed and compared.Results The MAAS score on 5 min after extubation of study group was lower than that of control group (P<0.05);the MAP and HR values immediately in the drawing or after extubation 30 min in study group were lower than control group (P<0.05 ).The incidence of shivering and the chill time in study group was lower and shorter than control group (P<0.05).Conclusion Dexmedetomidine could effectively reduce the recovery period of stress level,and reduce the incidence of shivering in treatment of patients with intravenous inhalation combined anesthesia,It is conducive to patient of vital signs stable in recovery period.

Objective To conduct the molecular epidemiologic analysis of Staphylococcus aureus (S.aureus) in the intensive care units(ICUs) and general wards and to compare their clinical characteristics.Methods Ninety-six clinically isolated strains of S.aureus(43 strains from the emergency intensive care unit(EICU) and neurosurgical intensive care unit(NICU) and 53 strains from the general wards) collected from Sepetember 2015 to April 2016 were performed the bacterial identification and antibiotic susceptibility test.The molecular typing was performed by adopting staphylococcal protein A (spa) typing method.Results Among 96 strains of S.aureus,the detection rate of methicillin-resistant S.aureus(MRSA) was 40.6%(39/96),which among 43 strains in ICU was 62.8%(27/43) and which among 53 strains in the general words was 22.6%(12/53).The resistance rates of strains from ICUs to gentamicin,levofloxacin,clindamycin,fosfomycin and minocycline were 23.3%,48.8%,46.5%,32.6% and 32.5% respectively,while which from the general wards were 7.5%,24.5%,18.9%,2.1% and 0% respectively.The Spa typing results showed that the main types of ICUs were t002,t091 and t311.The major epidemic strain was t002(n=16,37.2%) and mainly isolated from EICUs(12 strains),26 spa types were identified among the general wards trains,mainly were t189,t377,t571,t034,t091,t127.Conclusion The detection rate of MRSA in ICUs is higher than that in the general wards,these strains have high resistant rate to routine antibacterial drugs.t002 is the major epidemic strain.The general wards have more spa types with higher genetic diversity.