Object To study the source of sugar in flowers and fruits of Macrocarpium officinalis (Sieb et Zucc.) Nakai during fruiting.Methods The sugar contents were analyzed by HPLC.Results The contents of glucose, fructose and total sugar decreased slowly in the third or the forth branch after the barks were cyclically cut and there were significant differences between test group and control group, the changes of sugar contents in the first or the second branch were similar to control group.Conclusion These sugars in flowers and fruits were provided by the first or the second branch during fruiting.

Objective To study the regulation of dry substance and active components accumulation in the fruit of Forsythia suspensa. Methods The dry substance of the fruit was analyzed by the method of hundred grain mass. The contents of the active components in leaves, shucks, and seeds were analyzed by HPLC. Results The period for F. suspensa to accumulate the dry substance was mainly May and July. During the whole growth phase, the active components, such as forsythiaside, forsythin, and rutin in different parts, were decreasing. From the last ten-day of August to the first ten-day of September, the accumulation of the dry substance came to stability and the contents of active components in shucks and seed were stable. The contents of active components in shucks decreased rapidly from September to October, and then came to stability and the contents in shucks is the least during the last ten-day of October. So the contents of active components in younger F. suspensa are higher than that in elder one, and the proportion among forsythiaside, forsythins, and lution is also significantly different from elder seeds. Conclusion It is more preferable to pick younger F. suspensa from the last ten-day of August to the first ten-day of September and to pick elder F. suspensa during last ten-day of October.

In natural condition, two years were needed for Panax quinquefolius seeds to break dormancy. In the first year, the embryo developed slowly, and the activities of amylase and peroxidase were low. In the second year the embryo developed rapidly, and the activities of both enzymes also increased gradually. But with the treatment of hormones enzymatic activ ities accelarated remarkably and speeded up the decomposition of stored nutrients in endos perm, so that starch and non-reducing sugar decreased while reducing sugar incrcased rapidly, resulting in earlier germination.

A tumor-targeting recombinant fusion immunotoxin B-L-SEC2 was constructed by fusing staphylococcal enterotoxin C2 (SEC2) and an anti-HER-2 single-chain Fv B1 through a peptide linker, and expressed in E. coli strain BL21 (DE3) with an improved expression vector pASK75-EX as inclusion body. The denatured inclusion body was purified with Ni-NTA chelate agarose, and then re-natured by dialysis. FACS and MTT assays indicated that the re-natured fusion immunotoxin B-L-SEC2 could target the HER-2 over-expressing breast tumor cell SK-Br-3 in vitro, and inhibit the growth of SK-Br-3.

Objective To evaluate the safety and efficacy of percutaneous nephrolithotomy (PCNL) with B ultrasound-guided renal access in the lateral decubitus flank position for complex renal calculi. Methods From June 2004 to August 2009, 650 patients with renal stones≥20 mm underwent PCNL with B ultrasound-guided renal access in the lateral decubitus flank position. 512 men and 138 women,with a mean age of 38 years(range 11 - 78 years)and a mean stone size of 31 mm(range 20 - 58 mm). Results Successful access was achieved in 650 patients (100%). Minimally PCNL and PCNL were performed in 493 and 157 patients. Complete stone clearance rate was 86. 6% (563/ 650). The mean operative time was 72 min(range 35 - 145 min), and the mean hospital stay was 18d (range 9 - 32 d). There were no visceral injuries. Conclusions PCNL with B ultrasound-guided renal access in the lateral decubitus flank position is safe and convenient, and prevents harmful effects of radiation for the surgeon, the surgical team, and the patient.

Objective To summarize clinical outcomes of interventional therapy on children with common congenital heart diseases(CHD).Methods A retrospective study was conducted.One hundred and fourteen patients with CHD were selected as our subjects,who underwent catheter interventional therapy in the Second Affiliated Hospital of Zhengzhou University from Jan.2004 and Dec.2012.The size of occluder was chose according to intraoperative echocardiography or cardiac imaging measurements,and occluder was released under assisted monitoring by subtraction angiography or cardiac ultrasound.Results There are all together 112 patients got the therapy successfully,2 cases failed(occluder detachment),and the success rate of operation was 98.2％.After the success of interventional,echocardiography examination showed that 11 cases were with star point across shunt,but the function of the around valve was not affected.One months after operation,echocardiography examination showed star point across shunt of 11 cases were disappeared,and no occluder was shifted as well as no thrombosis formed.Three months after operation,chest radiograph showed pulmonary congestion decreases and heart shadow was shrink.Thirty-eight cases were with three tricuspid regurgitation before operation and 32 cases were without reflux at 3 months after operation,and 6 cases relieved significantly.The patients were followed up for 6 months or 3 years,activity endurance was significantly improved than that before operation.No occluder was shiftand hemolysis and arrhythmia occurred.Meanwhile,No thrombosis or embolism occurred.Conclusion Interventional treatment for children with congenital heart disease is proved as a safe,effective methods and it have broad prospects in clinical application.

To evaluate the differences of Ophiopogonjaponicus from different cultivations, the metabolomics based method was conducted to compare the effects of Hangmaidong and Chuanmaidong (Chinese name) on plasma endogenous metabolites of normal rats. Data were collected by ultra performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF/MS), and were analyzed by multivariate statistical method, such as Principal Component Analysis and Orthogonal Signal Correction Partial Least Square Discriminant Analysis. Results revealed that the plasma metabolites profiling of low and middle dose group of Chuanmaidong were similar to the control group, but different from the high dose group obviously. Meanwhile the high, middle and low dose groups of Hangmaidong were different from control group notably, and the difference is dose dependent. Lysophosphatidylcholines, the possible endogenous metabolites which contribute to the classification most significantly, are closely related to cardiovascular system diseases. Compared with the group of Chuanmaidong, Hangmaidong has greater impact on the plasma metabolic profiling of normal rats. Hangmaidong and Chuanmaidong showed significant differences pharmacodynamically.

Object To develop a method of multiplying virus free clonal seedlings of CV.85 5 of Rehmannia glutinosa (Gaert.) Libosch. ex Fisch. et Mey.. Methods The leaf cut segments with or without tips of plantlets were grown in different media (mg/L) (1.MS+BA 0.5; 2 MS+BA 0.5+NAA 0.02;3 MS+BA 0.5+NAA 0.02+GA 3 0.1; 4 1/2 MS+BA 0.1; 5 1/2 MS+BA 0.1+GA 3 0.1; 6.1/2 MS+GA 3 0.1) to select a suitable medium, and the height and the numbers of newly formed leaves and roots were recorded 30 days later. To screen out a favorable condition, similar segments were transferred to No.2 medium at different temperatures in different illuminations, and the height together with the leaf number and the fresh weight of the plantlets was recorded.Results In the former three media, the segments with tips were flourishing and one to three axillary buds were formed at its basal stem; while in those without tips almost every axillary bud developed with the main tip length 0.5～1.5 cm. In the later three media, thd elongation of the segments was usually overstimulated, and the slim plantlets with fewer leaves and more roots formed. When the segments cultured in No.2 medium, all records at 28 ℃ were higher than those at 23 ℃ in the same illuminations. But the leaf size and the height of the plantlets were inversely proportional to the intensity of illumination at the same temperature. In addition, antagonism between BA and GA 3 was found.Conclusion No.2 medium is more suitable for the multiplication of the virus free seedlings of 85 5 when cultured at 28 ℃ in the illumination of 1 000～2 000 lx.