1.Pharmacokinetic effect of combined administration on spinosin and ferulic acid in monarch drug Ziziphi Spinosae Semen kernel.
Rong GAO ; Shan LI ; Xian-jin CHEN ; Xiao-feng WANG ; Shi-xiang WANG ; Min-feng FANG
China Journal of Chinese Materia Medica 2015;40(16):3293-3297
To study the pharmacokinetic effect of different combined administration with monarch drug Ziziphi Spinosae Semen on its main components in rats. Sprague-Dawley (SD) rats were randomly divided into Ziziphi Spinosae Semen group, Ziziphi Spinosae Semen-Fructus Schisandrae Chinensis group, Ziziphi spinosae Semen-Salviae Miltiorrhize Radix et Rhizoma group and Zaoren Ansheng prescription group. After oral administration, HPLC was eluted with the mobile phase of acetonitrle-0.03% phosphate acid water in a gradient mode. The detection wavelength was 280 nm. The pharmacokinetic parameters of spinosin and ferulic acid were calculated by DAS 2. 0 software. Compared with Ziziphi Spinosae Semen group, Ziziphi Spinosae Semen-Fructus Schisandrae Chinensis group, Ziziphi Spinosae Semen-Salviae miltiorrhizae Radix et Rhizoma group showed a lower maximum plasma concentration (C(max)) and area under curve (AUC(0-t)) for spinosin and ferulic acid but higher clearance speed (CL/F); whereas the Zaoren Ansheng prescription group showed higher maximum plasma concentration (C(max)) and area under curve (AUC(0-t)) for spinosin and ferulic acid but lower clearance speed (CL/F). Compared with Ziziphi Spinosae Semen group, prescription group showed slower metabolism of spinosin and ferulic
Animals
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Chromatography, High Pressure Liquid
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Coumaric Acids
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administration & dosage
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blood
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pharmacokinetics
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Drug Interactions
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Drugs, Chinese Herbal
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administration & dosage
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analysis
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pharmacokinetics
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Female
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Flavonoids
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administration & dosage
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blood
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pharmacokinetics
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Humans
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Male
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Rats
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Rats, Sprague-Dawley
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Ziziphus
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chemistry
2.Comparative Study on Wrist-ankle Acupuncture Versus Physical Therapy for Lumbar Intervertebral Disc Herniation
Xian XU ; Fanfu FANG ; Feng HUANG ; Kaixuan YANG ; Qinghui ZHOU ; Wei GU
Shanghai Journal of Acupuncture and Moxibustion 2016;35(3):317-321
Objective To campare the clinical efficacy of wrist-ankle acupuncture versus physical therapy in treating lumbar intervertebral disc herniation (LIDH).Method Fifty LIDH patients were randomized into a treatment group of 20 cases and a control group of 30 cases. The treatment group was intervened by wrist-ankle acupuncture, while the control group was by traction and interference electrotherapy. The short-form McGill Pain Questionnaire (SF-MPQ) and lumbar pain scoring system by Japanese Orthopedic Association (JOA) were compared before and after intervention.Result The SF-MPQ and JOA scores were significantly changed respectively after 5-day, 10-day, 15-day treatment as well as at the follow-up study in both groups (P<0.01). There were no significant differences between the two groups in comparing the SF-MPQ and JOA scores respectively after 5-day, 10-day, 15-day treatment as well as at the follow-up study (P>0.05).Conclusion Wrist-ankle acupuncture and physical therapy both are effective in treating LIDH, as they both can release the pain.
3.Correlation between invasion and metastasis of gastric cancer and serum angiopoietin-2
Shao-ping LIU ; Chun-hua FANG ; Li-qun YU ; Xian-feng ZHANG ; Ya-hua HU
Chinese Journal of Postgraduates of Medicine 2011;34(28):31-34
Objective To study the clinical significance of the serum angiopoietin-2(Ang-2) in the diagnosis,recurrence,invasion and metastasis of gastric cancer.Methods The serum Ang-2 and carcino-embryonic antigen (CEA) levels in 158 patients with gastric cancer (gastric cancer group) and 30 normal controls(control group) were measured by enzyme linked immunosorbent assay(ELISA) technique respectively.The serum Ang-2 and CEA levels were also measured 2 weeks after operation in gastric cancer group and reexamined in the recurred gastric cancer patients in 2 years after operation (recurred and metastasis group).The correlation between the serum Ang-2 level and pathologic c haracterization of gastric cancer was evaluated.Results The serum Ang-2 and CEA levels in gastric cancer group [ (331.8 ±64.3),(42.6 ±37.3)μg/L] and recurred and metastasis group [(318.7 ±72.9),(40.5 ±36.7)μg/L] were significantly higher than those in control group [ (187.4 ± 32.7),(4.2 ± 3.1 )μ g/L] (P < 0.01 ),and the serum Ang-2 level 2 weeks after operation [ (211.6 ± 75.1 ) μ g/L ] was significantly decreased to the control group (P > 0.05 ),while the serum CEA level [ (33.4 ± 30.6) μ g/L ] was still significantly higher than the control group (P < 0.01 ).The sensitivity of the serum Ang-2 for diagnosis of gastric cancer was markedly higher than that of the serum CEA (P < 0.01 ).There was correlation between serum Ang-2 and degree of tumor differentiation,TNM pathological staging,lymphatic metastasis,invasion depth and tumor size (p <0.01 ),but there was no correlation between serum Ang-2 and tissue classification and location of gastric cancer (P> 0.05).Conclusion The serum Ang-2 level is suggested to be a valuable gastric cancer marker and conduce to the diagnosis of gastric cancer,the monitoring of recurrence after operation and evaluation of prognosis.
5.The comparison of efficacy of different dosage regimen of recombinant human tumor necrosis factor receptor-Fc fusion protein in Chinese ankylosing spondylitis patients
Hui-Qin HAO ; Feng HUANG ; Jie TANG ; Xiao-Hu DENG ; Ya-Mei ZHANG ; Ta-Lin SUO ; Xian-Feng FANG ;
Chinese Journal of Rheumatology 2001;0(04):-
0.05).In addition,in different medication intervals and the same total dosage(200 mg),there was no difference in the number of patients who reached ASAS20,ASAS50 anti BASDAI50 in both groups.The changes of other parameters were not observed.Conclusion Two dosages and different medication interval of rhTNFR-Fc have similiar efficacy onset time and maintenee period.Mean- while,at the same total dosage,there is no signifieant difference in therapeutic effect in the two dosage groups. However,50 mg(1/7 d)regimen has better compliance than 25 mg(1/3 d).
6.The preliminary investigation of tumor necrosis factor-a blocker treatment on CD4~+CD25~+ Treg cells (peripheral blood)in ankylosing spondylitis patients
Hui-Qin HAO ; Feng HUANG ; Xiao-Hu DENG ; Ya-Mei ZHANG ; Jie TANG ; Li-Ping PANG ; Xian-Feng FANG ;
Chinese Journal of Rheumatology 2000;0(06):-
Objective To investigate the role of CD4~+CD25~+ Treg cells on the pathogenesis of ankylos- ing spondylitis(AS), and to study the machanism of tumor necrosis factor(TNF)-?blocker on the treatment of AS by detecting the number of CD4~+D25~+ Treg cells before and after the treatment. Methods The diagno- sis of 10 AS patients was made based on the 1984 modified New York criteria. The patients received subcuta- neou injection of recombinant human tumor necrosis factor receptor-Fc fusion protein(rhTNFR-Fc)(etaner- cept)50 mg weekly for 8 weeks and 10 heathy subjects were enrolled for control. The mononuclear cells were isolated from peripheral blood in beth patients and controls. The number of CD4~+CD25~+ T cells and CD4~+ CD25~(high)T cells and the expression of CTLA-4. were detected by flow cytometry. Results The proportion of CD4~+CD25~+ T cells(24?19)% in total CD4~+ T lymphocytes of peripheral blood and CD4~+CD25~(high)T/CD4~+ T (6?6)% from AS patients before treated with rhTNFR-Fc was higher than that in healthy volunteers and AS patients after treatment(P
7.Effect of Jiawei Foshou San and its compatibility on hepatic P450 enzyme activity and hepatocyte morphology in rats.
Fang-hong SHANG ; Shan FENG ; Fei-yan ZHANG ; Qian CHEN ; Xian-jin CHEN ; Ji-fen ZHANG ; Xiao-yu XU
China Journal of Chinese Materia Medica 2015;40(10):2030-2036
To investigate the effect of Jiawei Foshou San and its various combined administration on hepatic P450 enzyme activity and hepatocyte morphology in rats. Rats were orally administered with drugs for four weeks and then sacrificed to prepare liver microsomes. The liver microsomes were incubated with the cocktail method; The metabolites were determined with the rapid liquid chromatography with tandem mass spectrometry (LC-MS/MS) to investigate the hepatocyte P450 enzyme activity. In addition, the hepatic pathological changes were observed by using the hematoxylin and eosin (HE) staining. Compared with the control group, the enzyme activity of CYP1A2 and CYP3A4 in the Jiawei Foshou san group showed a significant rise (P < 0.05); the enzyme activity of CYP1A2, CYP2C9, CYP2D6, CYP2E1 and CYP3A4 in the ferulic acid + ligustrazine group and the ligustrazine + tetrahydropalmatine group showed a significant rise (P < 0.05) ; the enzyme activity of CYP1A2, CYP2D6 and CYP2E1 in the ligustrazine group showed a significant rise (P < 0.05); the enzyme activity of CYP3A4 in the ferulic acid group showed a significant reduction (P < 0.05). After the administration with various drugs, the hepatocyte morphologies in the ferulic acid group and the ligustrazine group were normal. The pathological changes were observed in the tetrahydropalmatine group, such as unclear boundary of hepatic lobules, disordered hepatic cell arrangement, blurred edge, anisokaryosis and infiltration of inflammatory cells. The ferulic acid + tetrahydropalmatine group, the ligustrazine + tetrahydropalmatine group and the Jiawei Foshou San group also showed inflammatory infiltration, but with less pathological changes, particularly the Jiawei Foshou San group. The study result shows that Jiawei Foshou San can induce the enzyme activity of CYP1A2 and CYP3A4, and ligustrazine may be the effective substance for inducing CYP1A2. Its combination with ferulic acid and ligustrazine can significantly reduce the liver toxicity of tetrahydropalmatine.
Animals
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Cytochrome P-450 Enzyme System
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metabolism
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Drugs, Chinese Herbal
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administration & dosage
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Female
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Hepatocytes
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drug effects
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enzymology
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Microsomes, Liver
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drug effects
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enzymology
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Rats
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Rats, Sprague-Dawley
8.Binocular integration of binocular neurons in striate cortex of Kitten
Fang, XIE ; Xue-feng, SHI ; Li-min, XU ; Teng-yue, ZHANG ; Jia-xing, WANG ; Yu-xian, NING ; Kan-xing, ZHAO
Chinese Journal of Experimental Ophthalmology 2011;29(6):485-488
Background The integration of segregated pathways from the two eyes first appears in V1 neurons,where it not only plays a critical role in the generation of a three-dimensional visual representation.Abnormal visual experiences in critical period usually lead to amblyopia and binocular integration defects.Objective Present study was to investigate how neurons of kitten coordinate their activity patterns in response to synchronous dichoptic stimulus inputs in striate cortex.Methods Spike rate and local field potential(LFP) gamma band(20-90Hz) power of three kitten(1-1.2Kg,8-10 weeks old) to monocular and synchronous dichoptic presented gratings were assessed for 28 binocular neurons in V1 of kitten by in vivo extracellular record method under anaesthesia and paralysis.Ocular dominance index(ODI) and binocular integration index(BII) were assessed and the correlation between these two indexes were analyzed.Results In 28 cells with binocular characteristic,the absolute value of spike-ODI was significant larger than that of LFP-ODI(t=2.606,P=0.021).A positive linear correlation between the ocular preferences of spike and LFP was found(R2=0.513,F=27.423,P=0.003).In dichotic trails,binocular facilitation with BII for spike was 2.348±0.996,showing a significant reduce in comparison with BII for LFP(3.678±1.974)(t=2.671,P=0.019).Binocular integration index for two signals were greater when monocular responses of both eyes were similar(P=0.035 and P=0.124,respectively).Conclusion Both spike rate and gamma band power of LFP exhibited binocular facilitation to synchronous presented dichotic stimuli with significant facilitation induced by balanced monocular responses.Spiking activity and LFP reflect neural activities of different spatial scales and source components.
9.A prevalence survey on the incidence of genital warts between urban and rural areas in China.
Zhi-fang LI ; Shu-zhen QI ; Xiang-xian FENG ; Ju-fang SHI ; You-lin QIAO ; Xin-xin LI ; Qian-qiu WANG
Chinese Journal of Epidemiology 2010;31(9):1078-1079
Adult
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China
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epidemiology
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Condylomata Acuminata
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epidemiology
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Female
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Humans
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Incidence
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Male
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Retrospective Studies
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Rural Population
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Urban Population
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Young Adult
10.An investigation on knowledge,attitude,and willingness of human organ donation among undergraduate students in Hangzhou City
Qiang GAO ; Xian-Hong HUANG ; Qi FANG ; Chen-Feng MAO ; Lu-Yi FENG
Journal of Preventive Medicine 2015;(10):1003-1007
Objective To investigate the status of knowledge,attitude,willingness toward human organ donation and its influencing factors among undergraduate students in Hangzhou City.Methods Using stratified random sampling method, 626 students from six universities in Hangzhou were selected.Data was analyzed by statistical description,one -way ANOVA and multiple linear regression method.Results The average scores of knowledge,attitude and willingness were 8.47 ±2.68,89.33 ±1.44 and 9.09 ±2.37,respectively and those with a willingness of organ donation made up 16.61%.Interpersonal communication,traditional ideas,knowledge level and religious belief were the influencing factors (P <0.05 ).Conclusion Undergraduate students generally hold a positive view on organ donation while have a poor knowledge and willingness of it.Family,school,as well as the society should take effective measures to improve the level of knowledge,attitude and willingness of organ donation among undergraduate students.