Diplopia ; etiology ; Fracture Fixation ; adverse effects ; methods ; Humans ; Orbital Fractures ; diagnosis ; diagnostic imaging ; surgery ; Orbital Implants ; Tomography, X-Ray Computed

Objective To study the chemical constituents in ethanol extract from the carpophore of Xanthoceras sorbifolia and explore a new potential part.Methods The constituents were isolated by macroporous resin and silica gel column chromatography and their structures were elucidated by the phisi-cochemical properties and spectral evidence.Results Compounds Ⅰ-Ⅵ wre obtained and their structures were identified as 21-angeloyl-24-hydroxy-R1-barrigenol(Ⅰ),23-hydroxybetulinic acid(Ⅱ),3?,23-dihydroxy-lup-20(29) en-28-oic acid-3-caffeate(Ⅲ),22,23-dehydroxy-?-chondrillasterone(Ⅳ),3-oxotirucalla-7,24-dien-21-oic(Ⅴ),and ?-spinasterol-3-O-?-D-glucopyranoside(Ⅵ),respectively.Conclusion Compound Ⅰ is a new natural product and compounds Ⅱ-Ⅳ are isolated from the plants of Xanthoceras Bunge for the first time.

Objectives:To study the effect of triptolide(Tri) on fibroblast cells in vitro culture, morphologically and kinetically. Methods:Light microscope(LM)and electron microscope(EM)were employed to detect the morphological changes in Tri group. Cell DNA content and apoptosis were assayed by flow cytometry (FCM). Results:In Tri group, fibroblast cells showed less density, slimmer body, abnormally and typical apoptosis under LM, while rarefaction and content of rough endoplasmic reticulum(RER) decrease, and apoptosis in different stages could be seen under EM. In FCM,AI elevated remarkably in Tri groups, and the effect is dose dependent, with cell cycle uninfluenced at all. Conclusions:The mechanism of triptolide on hypertrophic scars is related to its promotion on fibroblast apoptosis and inhibition of collagen excretion.

Adult ; Arterial Occlusive Diseases ; etiology ; Arteritis ; complications ; Coronary Artery Disease ; etiology ; Female ; Humans

Animals ; Electroencephalography ; methods ; Entropy ; Epilepsy ; chemically induced ; physiopathology ; Male ; Nonlinear Dynamics ; Penicillins ; Rats ; Rats, Sprague-Dawley ; Signal Processing, Computer-Assisted

Posterior pedicle fixation-based dynamic stabilization is now densely studied in the non-fusion spine surgery. The method is characterized by the motion preservation of segmental lumbar, avoidance of the stress change after fusion surgery, and adjacent disc degeneration. Posterior pedicle fixation-based dynamic stabilization systems have undergone fast development and are now used for the treatment of degenerative lumbar spine disease. As an innovation of traditional fusion surgery, the clinical evaluation of its efficacy has become a focus of study among spine surgeons. In this paper, we review the recent progress in the clinical efficacy of posterior pedicle fixation-based dynamic stabilization.

Gout ; therapy ; Humans ; Hyperuricemia ; therapy ; Medicine, Chinese Traditional ; methods

Objective To explore the expressions of adiponectin and adiponectin receptor 2 (AdipoR2) in the liver of patients with hepatitis B virus (HBV)-related liver failure and their clinical implications in the pathogenesis of liver failure.Methods The healthy controls (HC),patients with chronic hepatitis B (CHB) and patients with HBV-related liver failure (HBV-LF) were enrolled in the study.Each group had 20 participants.The liver tissues from 11 CHB patients who were diagnosed by liver biopsy,6 patients with HBV-LF and 6 liver donors during liver transplantation were collected.Histological specimens were observed by hematoxylin-eosin staining and Masson trichrome staining under microscope.The mRNA and protein expressions of adiponectin and AdipoR2 in the liver were measured by semi-quantitative reverse transcription and polymerase chain reaction (SqRT-PCR) and immunohistochemical staining,respectively.The level of serum adiponectin was detected by enzymelinked immunsorbent assay.Serum biochemical parameters and HBV DNA levels were also measured.The pairwise comparison between groups was done by Student-Newman-Keuls and mann whitney U test.The relationship between two variables was analyzed using Spearman correlation.Results The level of serum adiponectin in HBV-LF group [(0.86 ± 0.15) ng/mL] was higher than those in HC [(0.59±0.15) ng/mL] and CHB groups [(0.62±0.13) ng/mL,Z=3.963,Z=3.774,both P＜0.01)],but showing no difference between CHB and HC groups (P＞0.05).The expressions of adiponectin and AdipoR2 mRNA in the liver were significantly higher in HBV-LF group (0.251 ±0.028 and 0.223 ± 0.021,respectively) than those in HC (0.091 ± 0.018 and 0.072 ± 0.020,respectively) and CHB (0.121±0.019 and 0.097±0.017,respectively) groups (q=18.428,17.069,19.807,18.673,respectively; all P＜0.01).Also,the expressions of adiponectin and AdipoR2 mRNA in CHB group were significantly higher than those in HC group (q=3.895,3.860,both P＜0.05).The expressions of adiponectin and AdipoR2 proteins in the liver were significantly higher in HBV-LF group (8.482±0.772 and 7.654±0.272,respectively) than those in HC (4.045± 0.815 and 2.804±0.623,respectively) and CHB (5.545±0.613 and 4.775±0.458,respectively) groups (q=15.327,11.542; Z=2.802,3.266; respectively; all P＜0.01).Also,the expressions of adiponectin,AdipoR2 proteins in CHB group were significantly higher than those in HC group (q=5.894,Z=3.266,both P＜0.01).In HBV-LF group,serum adiponectin levels as well as the expressions of adiponectin mRNA and protein in the liver were negatively correlated with serum albumin (r=-0.815,-0.886,-0.943; P＜0.01 or P＜0.05),but positively correlated with serum alanine aminotransferase (r=0.701,0.886,0.943; P＜0.01 or P＜0.05).The expression of AdipoR2 mRNA in the liver was negatively correlated with serum albumin (r=-0.943,P＜0.01),but positively correlated with serum aspartate aminotransferase (r=0.829,P＜0.05).Conclusions The expressions of adiponectin and AdipoR2 are up-regulated during HBV infection,especially in patients with HBV-LF,which might reflect the degree of necroinflammation in the liver.

In order to explore more reasonable and scientific accounting method of community health service costs, to improve the accounting method system of community health service cost. In the current existing community health services costing methods, based on the analysis of hospital cost accounting methods system, analyze and imitate the accounting method of hospital costs, establish a more scientific and implemented accounting method system of community health service costs: divide parts of clinics scientifically, redesign the center acollation method of indirect cost, confirm different cost accounting methods for different cost accounting objective and provide suggestion for the improvement of project cost accounting.

Adult ; Delirium ; chemically induced ; Female ; Humans ; Thiocarbamates ; poisoning