1.Research advances in corneal newborn lymphatic vessel and corneal transplantation rejection
Jie-Ying, MAI ; Xian-Ling, TANG ; Ping, LIU
International Eye Science 2014;(12):2168-2171
Corneal newborn lymphatic vessels construct the afferent arc of corneal immunological reaction, which play important role in immune response. The corneal transplantation rejection rate rises due to the emergence of new lymphatic vessel which breaks the immunologic mechanism. With the founding of specific marker of lymphatic endothelial cells and research advancing of growth factor of lymphatic vessels, the mechanism, therapy and prevention of corneal immunological rejection reaction of corneal lymphatic vessel have been studied intensively. The graft survival rate has been greatly improved through inhibiting newborn lymphatic vessel.
2.Variation of polysaccharides and alcohol-soluble extracts content of Dendrobium officinale.
Qiao-xian YU ; Ying-ying GUO ; Jin-ping SI ; Ling-shang WU ; Lin-hua WANG
China Journal of Chinese Materia Medica 2014;39(24):4769-4772
To reveal the variation of polysaccharides and alcohol-soluble extract contents of Dendrobium officinale, the polysaccharides and alcohol-soluble extracts contents of three D. officinale strains were determined by phenol-sulfuric acid method and hot-dip method, respectively. The results showed that the contents of polysaccharides and alcohol-soluble extracts and their total content were significantly different among D. officinale samples collected in different periods, and the variations were closely related to the phenology of D. officinale. Additionally, the quality variation of polysaccharides was closely related to the flowering of D. officinale, while the alcohol-soluble extracts was closely associated to the formation and germination of buds. According to the dynamic variation of these two compounds, it is more reasonable to harvest D. officinale at biennials pre-bloom than at specific harvesting month considering polysaccharides content. It is better to harvest before the germination of buds considering alcohol-soluble extracts. While with regards to both polysaccharides and alcohol-soluble extract, it is better to harvest this plant at the period from the sprouting to pre-bloom next year.
Dendrobium
;
chemistry
;
Plant Extracts
;
isolation & purification
;
Polysaccharides
;
isolation & purification
3.The Value of Reading Report in Training of Medical Students' Integrated Faculties
Ying XIAN ; Jie TIAN ; Mo WANG ; Jing ZHANG ; Jin-Ping LIANG ; Jie YU ;
Chinese Journal of Medical Education Research 2006;0(10):-
Objective:To explore the value of reading report in upbringing the integrated faculties of medical students.Methods:To give the training of reading report to the Seven-year and the five-year program medical students in their last year.Results:Such a training stirred the students' learning interests,improved their abilities in independent study,promoted their ability in collecting and dealing with information and widened their knowledge.Medical students grasped of the essential methods in writing reading report,which settled the groundwork for research abilities in the future.Conclusions:Reading report writing is instrumental in de- veloping students' integrated faculties and worthy advocating in medical teaching.
4.Pterygium conjunctive reverse transplantation combined with amniotic membrane transplantation on recurrent pterygium
Ting, LI ; Shu-Xian, CHEN ; Xu-Guang, XIA ; Zheng, YIN ; Mu-Zhen, HUANG ; Ping-Ying, GUO
International Eye Science 2014;(9):1715-1716
To discuss the effective method of decreasing the postoperative recurrence rate of recurrent pterygium.
●METHODS:Totally 126 cases (126 eyes) with recurrent pterygium were randomly divided into A group (56 cases) and B group ( 70 cases ). Group A was treated by pterygium conjunctive reverse transplantation combined with amniotic membrane transplantation, group B was treated by amniotic membrane transplantation. The followed-up time after surgery was 6-24mo.
●RESULTS:ln group A, postoperative 5-7d (average 5. 62± 1. 38d), cornea epithelium was repaired. ln group B, postoperative 7- 10d ( average 7. 38 ± 1. 12d), the corneal wound was healed. There was statistical significant difference between two groups (t = 4. 307,P<0. 05). Three cases recurrence were noted in A therapeutic group (56 cases), the recurrent rate was 5. 4%; Twelve cases recurrence were noted in B compared group (70 cases), the recurrent rate was 17. 1%. There was statistical significant difference between two groups(P<0. 05).
●CONCLUSlON: lt is suggested that pterygium conjunctive reverse transplantation combined with amniotic membrane transplantation is effective in the treatment of recurrent pterygium.
5.Expression of cytokines in CD8~+ T lymphocytes of the peripheral blood of patients with condyloma acuminatum
Dong-Xian LIU ; Wen JIANG ; Xing-Ping CHEN ; Ying-Ling CHEN ; Dong-Liang YANG ;
Chinese Journal of Dermatology 2003;0(11):-
Objective To detect the levels of cytokine IL-2,IL-12,IFN-?and IL-4 secreted by peripheral CD8~+ T lymphocytes in patients with condyloma acuminatum (CA).Methods Flow cytometry was employed to study the expression of cytokines IL-2,-12,INF-?and IL-4 in CD8~+ T lymphocytes in the peripheral blood of 60 patients with CA and 20 healthy controls.Results The percentage of CD8~+ T lym- phocytes producing IL-2,IL-12 and IFN-?were significantly lower in CA patients than that in healthy con- trols (P
6.Microsequencing, analysis of molecular weight and amino acid composition for pyrimidine 5'-nucleotidase I of human erythrocytes.
Zhu-Lin PAN ; Jin-Ying LI ; Bi-He MIN ; Kang YING ; Hong ZHOU ; Xiao-Ping XU ; Xian-Min SHONG ; Feng-Lai HAN ; Wei-Ping ZHANG ; Xian ZHANG
Journal of Experimental Hematology 2003;11(1):61-65
To further explore the mechanism of congenital pyrimidine 5'-nuleotidase I (P5'N-I) deficiency, on the basis of purification of the protein, the molecular weight and amino acid composition were analysed by mass-spectrograph and amino-acid analyzer, microsequencing and bioinformation analysis of P5'N-I were performed after it was hydrolysed by trypsin. The results showed that three fractions were found in the purified P5'N-I and their molecular weights were 26,952.9, 55,476 and 110,938, respectively. The sequence from one of the peptide fragments was I-E-G-P-T-I-R-Q-I-E. The homologous sequence was not found after comparision with the ten-amino-acid sequence in GenBank by blast procedure. Amino acid analysis indicated that P5'N-I was composed of 18 amino acids at least, and 243 amino acid residues. In conclusion, the enzyme might be an allosteric enzyme, there might be homologous dimer or tetramer in physiological status of normal human erythrocyte, the microsequence could be designed as the probe for fishing the genes of interest. The composition of amino acid might be an important information in determination of its protein primary structure.
5'-Nucleotidase
;
blood
;
chemistry
;
isolation & purification
;
Amino Acid Sequence
;
Amino Acids
;
analysis
;
Chromatography, High Pressure Liquid
;
Electrophoresis, Polyacrylamide Gel
;
Erythrocytes
;
enzymology
;
Humans
;
Mass Spectrometry
;
Molecular Weight
;
Peptide Fragments
;
chemistry
;
Sequence Analysis, Protein
7.Multiplex sequence-specific PCR detection for simultaneous screening of 5 types of JAK2 mutations in myeloproliferative diseases
Hongxing LIU ; Chunrong TONG ; Peng CAI ; Guirong TANG ; Xian ZHANG ; Wen TENG ; He WANG ; Ying ZHANG ; Ping ZHU
Chinese Journal of Laboratory Medicine 2009;32(1):30-34
Objective To develop a multiplex sequence-specific PCR assay for simultaneous screening of 5 types of JAK2 mutations and investigate its clinical application value. Methods Multiplex sequence-specific PCR assay for simultaneous screening of JAK2 V617F, K539L (include 2 types of gene mutations), N542-E543del and E543-D544del mutations were developed. 115 patients with myeloproliferative diseases (MPD) including 61 polycythemia vera (PV) cases, 43 essential thrombocythemia (ET) cases and 11 primary myelofibrosis (MF) cases were analyzed. Results The assay can screen the 5 types of JAK2 mutations efficiently. The detection sensitivity is 1% for JAK2 V617F mutation and 0.1% for the other mutations. JAK2 V617F mutation and JAK2 exon12 mutation were detected in 56 and 3 of the 61 PV samples, respectively. 27 of the 43 ET samples and 6 of the 11 MF samples were JAK2 V617F positive, but no JAK2 exon12 mutation was found in both groups. The 3 cases carrying JAK2 exon12 mutation had the clinical feature of erythrocytosis and erythropoietin-independent erythroid colony formation but without apparent leukocytosis, thrombocytosis and splenectasis. Conclusion The assay can simultaneously screen 5 types of JAK2 mutations with high sensitivity and thus lead to an increased detection rate.
8.Study on identification of "Digeda" raw materials in Mongolian patent medicine by PCR amplification of specific alleles.
Zhan-hu CUI ; Xian-zhang HUANG ; Ping LONG ; Le ZHANG ; Dong-dong ZHAO ; Ying-li WANG ; Min-hui LI
China Journal of Chinese Materia Medica 2015;40(5):793-798
To explore a new method for identification of Mongolian patent medicine (MPM) by PCR amplification of specific alleles. Eight kinds of MPM were used to study the identification of "Digeda" raw materials. The total DNA of Lomatogonium rotatum and Corydalis bungeana samples were extracted through modified CTAB method, psbA-trnH sequence was amplified by PCR and sequenced directionally. Specific primer was designed. The DNA of 8 kinds of MPM also was extracted and purified by the commercial DNA purification kits. The rbcL and two pair of specific primers sequences were amplified. The specific amplified products were sequenced in forward directions. All specific sequences were aligned and were analyzed. The results indicated that L rotatum can be identified by specific primers from Digeda-4 Tang, Digeda-8 San, Digeda-4 San, and C. bungeana medicinal materials can be identified by specific primers from Li Dan Ba Wei San, Yi He Ha Ri-12 and A Ga Ri-35. PCR amplification of specific alleles can stably and accurately distinguish raw medicinal materials in MPM.
Alleles
;
DNA Primers
;
genetics
;
DNA, Plant
;
genetics
;
Medicine, Mongolian Traditional
;
Molecular Sequence Data
;
Plants, Medicinal
;
classification
;
genetics
;
Polymerase Chain Reaction
;
methods
9.Observations on pathogenicity between A and B subtype of hMPV in mice
Zhen ZHOU ; Yuxia CUI ; Yongbo ZHANG ; Xian QIN ; Rongpei LI ; Ping LIU ; Ying DOU ; Lijia WANG ; Xiaodong ZHAO ; Yao ZHAO
Chinese Journal of Microbiology and Immunology 2012;32(1):36-40
ObjectiveTo investigate the difference of pathogenicity between the two genotypes of human metapneumovirus(hMPV) for the further research.MethodsAt various time after hMPV infection in BALB/c mice,viral titers of lung tissue were measured by real-time RT-PCR,pathology was assessed by a histopathological scoring system,airway responsiveness was assayed by animal lung function monitoring equipment.Pathogenicity was then measured by detailed evaluation through the results above.Results There is no significant difference in weight of mice between control group and experimental group through dynamic monitoring ; though the difference was exists in airway responsiveness and pathological changes of mice between control group and experimental group,the differences were not statistically in airway reaction,pathological changes and virus drops among the three groups of experimental group.ConclusionThere is no difference in pathogenicity between the two subtypes of hMPV in infection of BALB/c mice,viral genotype do not appear to be associated with pathogenicity.
10.A comparative study of the influence of different bowtie of 64 multi-slice CT on cardiac image quality and radiation dosage
Jian-Hua GAO ; Xian-Chang SUN ; Jian-Ying LI ; Na LI ; Qing-Tang XIA ; Wen ZHAO ; Ru-Ping DAI ;
Chinese Journal of Radiology 2001;0(08):-
0.05 indicating no statistical difference.However,the noise measurements for the L and C groups were 30.05 and 27.80,respectively,with P