Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; Gene Expression Profiling ; Humans ; Oligonucleotide Array Sequence Analysis ; Pancreatic Neoplasms ; drug therapy ; metabolism

Objective To evaluate the effects of intraoperative intraperitoneal chemotherapy with sustained release 5-fluorouracil for implantation(5-Fu SRI) for the treatment of locally advanced colon cancer. Methods A total of 70 patients with locally advanced primary colon cancer(T4N0M0 and stage Ⅲ) undergoing radical resection were divided into treatment group(n=36,intraoperative intraperitoneal chemotherapy with 600mg 5-Fu SRI)and control group(n=34,intraoperative intraperitoneal flushing with 1000mg 5-fluorouracil injection). Both groups received the same postoperative chemotherapy regime.The 2-year survival rate,local recurrence rate and distant metastasis rate were retrospectively analysed. Results The 2-year survival rate and local recurrence rate were more favourable in treatment group than those in control group(P0.05). Conclusion Intraoperative intraperitoneal chemotherapy with 5-Fu SRI may decrease postoperative local recurrence rate and increase 2year survival rate.

Objective To study the expression levels of interlukin-18(IL-18) in the CA1 region of the hippocampus and habenular of the rats with chronic stress. Methods Male Wistar rats (n = 30) weighting about 185～255g were divided into test group (T, n = 15) and control group (C, n = 15) randomly. The rats in group T were exposed to various types of stresses every day for consecutive 21 days. The rats in group C did not receive any stress during 21 days. Weighting and open-field tests were carried out on each rat before the test and on the 22nd day. On the 22nd day, brains were removed and cut coronally. Immunohistochemistry method was used to measure the expression levels of IL-18. Results After 21 -day stress, the body weight, erection time, rearing times, horizontal crossing numbers,modifying times and defecation in group T((297.33 ±25.83)g,(5.14 ±2.02)s,(19.00 ± 9.01), (9.47 ±3.64),(3. 93 ± 1. 87)and(4. 93 ± 1. 94)) were significantly different from those of group C((322.00 ±30.34)g,(1.97 ±0.93)s,(39.80 ±18.58),(14.80 ±5.88),(7.27 ±2.87)and(1.93 ±1.16)) (P < 0.01, P < 0.05). The average optical density values of IL-18 positive cells in CA1 region and habenular in group T ((0.3923 ±0.0084 and 0.4577 ±0.0234)) were higher than that in group C ((0. 3165 ±0.0063 and 0. 3400 ±0.0097)) (P<0.01). Conclusion The expression levels of IL-18 of the hippocampus glial cells and of the habenular neurons in the rats is increase after the chronic stress.

0.05), respectively. Conclusions Above data indicated that the ETB 2 could significantly restrain the development of mice liver cancer, and the effects depended on the time of administration. The mechanism may be due to its' effects of antiangiogensis, increasing necrosis and decreasing proliferation. Bifidobacterium has no significant antitumor function but could improve the immune function. Bifidobacterium may cooperate with endostatin in antitumor process.

Objective To explore the incidence rate, total incidence numbers and possible impacting factors of non-motor symptoms (NMSs) in Parkinson's disease (PD). Methods The NMS questionnaire (NMS Quest) was used to investigate 131 out-patients diagnosed with PD, and the prevalence of each item, the total NMS numbers and their relationships with clinical features were assessed. Results All of the patients, who were distributed in every stage of PD, had NMSs and each case with 11 items on average. Autonomic symptoms such as nocturia and constipation were the most frequent ones. The patients over 65 versus less than 65 years old had a higher prevalence in a number of items such as olfactory disturbance, dysphagia and constipation. At the same time, the rigidity subtype patients had a higher prevalence in depression items than the tremor subtype ones.The total number of NMS was positively correlated with course of disease, age, UPDRS score and Hoehn & Yahr (H&Y) stage. Conclusions NMSs are common among PD patients. While the prevalence of each item may vary with different clinical features, the total number of NMS is increased with the severity of PD. More attention should be paid to the diagnosis and rational treatment of the NMSs.

A certain amount of potassium chloride is required to correct the hypokalemia occuring commonly during perioperation,but the overdosage may be harmful. To investigate the proper dose of KCl,40 adult rabbits were randomly and evenly assigned to be intravenously infused with normal saline(group Ⅰ),or saline containing 4,25 or 50 mmol/L KCI (group Ⅱ,Ⅲ,Ⅳ),at rate of 10 ml?kg~(-1)?h~(-1) during operation respectively. As compared with those before operation,the serum level of potassium decreased significantly in group Ⅰ and Ⅱ, changed unsignificantly in group Ⅲ,and increased in group Ⅳ during and after operation;the potassium amounts in liver and skeletal muscles increased markedly after operation. S-T segment was depressed in group Ⅰ and Ⅱ,T wave became peaked in group Ⅳ, and ECG remained normal in group Ⅲ. it is indicated that intravenous KCl infusion at concentration 25 mmol/L and rate of 10ml?kg~(-1)?h~(-1) may properly correct the hypokalemia during perioperation,and the occurance of perioperatine hypokelemia may be related to the uptake of liver and skeletal muscles

Ten mongrel dogs,divided randomly into a control group (A) and a cerebroplegia group (B) ,were supported by closed-chest cardiopulmonary bypass,subjected to 2 hours of hypothermic circulatory arrest at 18 C. In group B, oxygenated asanguineous solution 50ml/kg was infused into the carotid artery at the onset of arrest. and 10 ml/kg was supplemented every 30 minutes during 2hours arrest. Nothing was infused in group A. Then all animals were rewarmed to 37 C and killed 6 hours later At five time points cerebral cortex was collected to study adnosine triphosphate (ATP),malondialde hyde (MDA) and ultrastructure. Electroencephalography (EEG) was recorded continuously. In group A, ATP content decreased gradually from the beginning of arrest to the end of the experiment (P

UNLABELLED: Patients with cervical painless mass for 3 months with swallowing not feeling a week for the chief complaint. PHYSICAL EXAMINATION top right sternocleidomastoid before hitting a 7.0 by 3.0 cm size, lower limb reached 2.0 cm x 1.8 cm, the size of the mass on the left side of the supraclavicular reached 3.0 cm x 2.5 cm of the size of the mass, the three homogeneous medium hard, focally border and clear, the activity can be puncture cytological examination in return for: left supraclavicular see more protein and blood samples and a small amount of sample are arranged heap of fiber cells. Nuclear magnetic resonance (NMR): on the right side of the neck, with three at the left supraclavicular neoplasm, between 2.5-5.5 cm in size, high in T2, T1 low mixed signals, lesion boundaries clear.
Clavicle ; Deglutition Disorders ; etiology ; Head and Neck Neoplasms ; complications ; pathology ; Humans ; Magnetic Resonance Imaging ; Meningeal Neoplasms ; pathology ; Meningioma ; pathology ; Neck ; Neurilemmoma ; complications ; pathology ; Sensation Disorders ; etiology ; Tumor Burden

AIM: To observe the curative effect of PDT combined with intravitreal injection of ranibizumab treatment for age-related macular degeneration with choroidal neovascularization ( CNV) .
METHODS:In accordance with the inclusion criteria, by indocyanine green choroidalangiography ( ICGA ) and optical coherence tomography ( OCT ) examination confirmed the diagnosis of macular CNV in 27 patients (27 eyes), treated with PDT 3 ~ 7d professional intravitreal injection of ranibizumab. At 1, 3, 6mo after treatment, the results of best corrected visual acuity (BCVA), FFA, ICGA, OCT examination and complications were observed.
RESULTS: The BCVA improved in 17 eyes ( 63%) , stable in 6 eyes ( 22%) , and decreased in 4 eyes ( 15%) . Before treatment, the average leakage area was 1 005. 69±105. 47μm, it were 875. 54 ± 103. 27μm, and 423. 37 ±79.68μm at 1 and 3mo after treatment, there were significant differences compared with before treatment ( P<0. 01). Average central macular thickness of retina before treatment was 485. 58±122. 59μm, and 398. 84±105. 32μm, 297. 74±89. 18μ m at 1 and 3mo after treatment, there were significant differences compared with before treatment( P<0. 01).
CONCLUSION: The method that PDT closed CNV combined with intravitreal injection of ranibizumab can effectively block angiogenesis recurrence, reduce the number of PDT treatment again and complications, improve the therapeutic effect.