The effect of inducers on leukemia cell line K562 was studied in liquid culture system in vitro. Our experimental results showed that hemin induced cells only to produce hemoglobin without suppressing the growth of cells; Butyric sodium inhibited only the growth of cells without induction of hemoglobin; Cytosine arabinoside and harring-tonine could induce the hemoglobin of cells in their toxicant quantification.The results suggest that hemin and butyric sodium may have not be a therapeutic effect on leukemia patient; The effect of induced differentiation and cell toxicity of cytosine arabin- oside and harringtonine may be closely interconnected. Clinically, there is still the possibility of cell toxicity in treatment of leukemia patients with small dosage of those drugs, and the effect of those drugs may be the combination of cell toxicity, induced differentiation and cell sensitivity.

Objective To evaluate the value of 99mTc-Octreotide somatostatin receptor and 99mTc-MIBI imaging in the detection of breast cancer. Methods 99mTc-Octreotide and 99mTc-MIBI imaging were performed in 26 patients with breast masses before operation. The scintigraphy results were analysed compared with pathologic study.Results The sensitivity, specificity and accuracy of 99mTc-Octreotide scintigraphy for breast cancer were 94.4%, 87.5%and 92.3%respectively and those of 99mTc-MIBI were 88.9%, 75.0%and 84.6%respectively. Significant difference was found between 99mTc-Octreotide and 99mTc-MIBI in both of specificity and accuracy (P

Objective: To improve the present method for casualty forecasting using the risk analysis theory. Methods: Empirical data were extracted from 11 urban aggressive combats during and after WW II, and the distribution of daily casualty was determined. Based on quantitative judgment model, several factors such as the number of soldiers, terrain, weather, general situation, suddenness, and combat efficacy were configured; Monte Carlo simulation was applied for simulation,and a forecasting model was setup with Microsoft Excel and Crystal Ball 2000 software for risk analysis of the simulation outcome. Results: The distribution of daily casualty during urban aggressive military action could be represented by normal distribution. With the values of the aforementioned factors, the result of 1000 tests showed that the mean daily casualty rate was 0. 42%, with the standard deviation being 0. 21%. Conclusion: Monte Carlo simulation is an effective means to improve the present casualty forecasting method.

Adult ; Female ; Humans ; Motor Neuron Disease ; complications ; Neck ; pathology ; Thyroid Neoplasms ; complications

Objective To investigate sexual dysfunction and its rehabilitation,as well as quality of life in patients after spinal cord injury(SCI). Methods Sixty-two male patients with SCl were assessed with a 5-item version of the International Index of Erectile Function(IIEF-5),an especially-designed sexual function scale,and the World Health Organization's quality of life questionnaire(WHO-QOL).Their results were compared with those of 146 other patients with work injuries hospitalized in the same period.Results 77.9% of 62 cases had moderate to severe erectile dysfunction.though more than half of them remained sexually active.52.7% had attempted sexual intercourse,but only 19.5% succeeded,and only 14.5% obtained sexual satisfaction.69.1% of the 62 patients had psychological symptoms,and 27.27% had family problems.Their average score on the WHO-QOL was significantly lower than that of the other disabled workers.There were significant differences between the two groups with regard to quality of life.especially in the physiological and social relationships domains. Conclusion Most male patients with SCI had sexual dysfunction and decreased quality of life.Sexual dysfunction rehabilitation of SCI patients needs urgent strengthening.

Theory of Chinese materia medica (CMM) nature was an important part in traditional Chinese medicine (TCM) theoretical system. During the long term practice, based on TCM theories of yin and yang, five elements, zang-fu, meridian and treatment principle, herbal features and patients' reactions were highly summarized into recognition and understanding. Since ancient time, theory of CMM nature has always guided the accurate syndrome differentiation and medication in TCM clinical practice. It contributed to the healthcare and reproduction of Chinese people. Theory of CMM nature is the basic theoretical knowledge which must be mastered in the study and research. Its concepts contained four-qi, five tastes, channel tropism, ascending and descending, toxicity, tonification and reducing, moisture and dryness, smooth and astringent, softness and hardness, fixed and itinerant effect, strong and mild. Using fixed and itinerant effects in the summarization of CMM function is one of the components in CMM nature theories. Although discussions on this theory from practitioners during past dynasties were in disorder, the significant guidance in herbal interaction mechanism and prescription compatibility should not be ignored.

In the experiment, the production of plagues by Bdellovibrio bacteria in solid medium cultivation, the reproduction of Bdellovibrio bacteria in liquid medium cultivation and the attachment of Bdellovibrio bacteria to carrier were observed, which aimed to study the effects of enrofloxacin on the growth and at-tachment ability of Bdellovibrio bacteria BDF-H16. Results indicated that in solid medium cultivation, the production of plagues by Bdellovibrio bacteria BDF-H16 was inhibited by different concentrations (2 ?g/mL, 5 ?g/mL, 10 ?g/mL, 20 ?g/mL, 50 ?g/mL) of enrofloxacin and the inhibitory effects of enrofloxacin became stronger with the increase of the concentration of enrofloxacin. Similarly, in liquid medium cultivation, the reproduction of Bdellovibrio bacteria BDF-H16 was also obviously inhibited by different concentrations ofenrofloxacin and higher concentrations of enrofloxacin such as 10 ?g/mL, 20 ?g/mL, 50 ?g/mL had stronger inhibitory effects on the reproduction of BDF-H16. However, the growth tendency of Bdellovibrio bacteria BDF-H16 was not inhibited in 10 ?g/mL enrofloxacin. Additionally, when zeolite was added, enrofloxacin had also inhibitory effects on the numbers of Bdellovibrio bacteria BDF-H16 attached to zeolite. With the increase of the concentrations of enrofloxacin, the numbers of Bdellovibrio bacteria BDF-H16 attached to zeolite became smaller and smaller. However, the attachment rate of Bdellovibrio bacteria BDF-H16 to zeo-lite became higher under 2 ?g/mL-20 ?g/mL enrofloxacin. The results above showed that enrofloxacin had inhibitory effects on the plague production and reproduction of Bdellovibrio bacteria BDF-H16, but the at-tachment ability of Bdellovibrio bacteria BDF-H16 was strengthened in liquid medium cultivation with 2 ?g/mL-20 ?g/mL enrofloxacin and zeolite, and adding zeolite helped to reduce the adverse effects of en-rofloxacin on Bdellovibrio bacteria BDF-H16.

OBJECTIVETo construct a cDNA library of osteosarcoma SAOS-2 cells and screen the proteins interacting with the bone morphogenetic protein-2 (BMP-2) to explore the role of BMP-2 in the development of prostate cancer.

METHODSThe total RNA was extracted from SAOS-2 cells, from which poly(A)(+)RNA was purified to generate the cDNA using RT-PCR with primer SMARTIII and primer CDSIIIoligo(dT). The library was constructed and screened by cotransformation of the double-stranded cDNA (dscDNA) and linearized pGADT7-Rec with the bait plasmid to the yeast AH109. The proteins interacting with BMP-2 were screened by AH109 mating with the bait strain Y187, and the interaction was confirmed using far-Western blot analysis.

RESULTSThe cDNA library of human osteosarcoma SAOS-2 cells was constructed, which was characterized by high diversity and sufficient capacity. The dscDNA size range was between 250-5000 bp, with a cotransformation efficiency of 4.3 x 10(5) and recombination efficiency of 1.9 x 10(6), and 4.3 x 10(5) clones were screened. The mating efficiency was 32% and 1.0 x 10(6) clones were screened by the bait of BMP-2, and 4 positive clones was obtained and sequenced.

CONCLUSIONThe diversity and capacity of the cDNA library meet the needs for screening genes related to prostate cancer.

Bone Morphogenetic Protein 2 ; genetics ; Cell Line, Tumor ; Gene Library ; Humans ; Male ; Osteosarcoma ; genetics ; Prostatic Neoplasms ; genetics ; RNA, Neoplasm ; genetics ; Two-Hybrid System Techniques

AIM: To investigate the effect of canstatin on cultured rabbit vascular smooth muscle cells(VSMC). METHODS: By means of cationic liposome mediated method, canstatin RNA was transferred into cultured VSMC. The proliferation quantity of VSMC were determined by the cell counting method and thymidine(-TdR) incorporation. RESULTS: Canstatin RNA could be effectively transferred into cultured primary rabbit aortic smooth muscle cells by the cationic liposome-Dosper and could markedly inhibit VSMC proliferation. CONCLUSION: Transfection of canstatin RNA could inhibit the growth of VSMC in vitro.

OBJECTIVE To drew the map of distribution of concealed sites in epistaxis and optimize the mode of diagnosis and treatment. METHODS We have searched 117 references on intractable/refractory/latent epistaxis treated under nasal endoscopy in the last decade, including 11 708 cases with epistaxis. There were total 11 860 cases in this study plus 152 cases in our hospital. We summed up the experiences of the optimized treatment mode performed on concealed epistaxis, which was searching the accurate bleeding areas by nasal endoscopy and performing minimally invasive radiofrequency treatment without nasal packing. RESULTS We had found that the offending arteries about epistaxis are mainly sphenopalatine artery and anterior or posterior ethmoid artery. The frequency of bleeding areas came as follows: the vault of inferior nasal meatus in 3783 cases(31.90%), the olfactory sulcus of middle turbinate in 3522 cases(29.70%), the posterior regions of middle meatus in 1349 cases(11.37%), the regions of deviation of nasal septum in 1065 cases(8.98%), the foremost regions of nasal cavity in 738 cases(6.22%), and the others or uncertain in 1403 cases(11.83%). Finally, we drew a concise map of distribution about epistaxis on the basis of the concealed bleeding areas and offending vessels. 151 of 152 patients(99.34%) could find out the bleeding sites accurately and stop the bleeding through minimally invasive radiofrequency ablation. CONCLUSION We drew a map of distribution about epistaxis in concealed areas so that it is convenient for us to seek out the bleeding sites. Rational use of nasal endoscopy to explore the sites of intractable epistaxis and minimally invasive radiofrequency are optimized mode of treatment.