OBJECTIVE:To make clear the situation of antimicrobial therapy for senile patients with lower respiratory tract infections and to evaluate the rationality of medication.METHODS:The data of the use of antimicrobials for in-hospital senile patients with lower respiratory tract infections were collected and the cost,DDD and DUI were analysed and evaluated.RESULTS:The average cost of antimicrobials for each patient was 2 257.86 yuan,which accouted for 25% of medical expense of hospitalization and 46% of total expense for drug.The average therapeutic course was(24.1?8.1) days.109 cases were cured and 6 cases were improved with a curative rate of 92.4%.CONCLUSION:The clinical use of antimicrobials in this series was rational on the whole.

Objective To explore the effect of Wendan Decoction plus Saffron in the treatment of patients with endometriosis of uterus on serum matrix metalloproteninases-9(MMP-9).Methods 74 cases of endometriosis of uterus were selected and randomly divided into two groups, 37 cases in each group.The control group were treated with oral administration of Gestrinone Capsules, the research group were treated on the basis of the control group with Wendan Decoction plus Saffron treatment,two groups were treated for 24 weeks.The serum estradiol, MMP-9 and matrix metalloproteninases inhibitor-1(TIMP-1) were detected before and after treatment, the degree of pain and clinical efficacy were compared.Results Compared with before treatment, levels of estradiol(E2),progesterone(P) and luteotropic hormone(LH) decreased in two groups(P<0.01), the levels of MMP-9, MMP-9/TIMP-1 decreased(P<0.01), levels of TIMP-1 increased(P<0.01), levels of CA125 decreased(P <0.01), and the maximum diameter of pelvic mass line decreased(P<0.01), dysmenorrhea pelvic pain, dyspareunia and VAS score decreased(P<0.01).Compared with the control group, levels of E2, P and LH in the research group were lower(P<0.01), the levels of MMP-9, MMP-9/TIMP-1 were lower(P<0.01), levels of TIMP-1 were higher(P<0.01), levels of CA125 were lower(P<0.01), the maximum diameter of pelvic mass line were lower(P<0.01), dysmenorrhea and pelvic pain and VAS scores were lower(P<0.01), and the effective rate of research group was higher(P<0.05).Conclusion Wendan Decoction plus Saffron in the treatment of endometriosis of uterus was effective, and it can reduce endometriosis, dysmenorrhea and pelvic pain degree, reduce pelvic mass, and may reduce the level of serum MMP-9 related.

2-Aminoadipic Acid ; analogs & derivatives ; analysis ; Aldehyde Dehydrogenase ; genetics ; Anticonvulsants ; therapeutic use ; Biomarkers ; analysis ; Brain ; pathology ; DNA Mutational Analysis ; Electroencephalography ; Epilepsy ; diagnosis ; drug therapy ; genetics ; Genetic Association Studies ; Humans ; Infant ; Infant, Newborn ; Magnetic Resonance Imaging ; Mutation, Missense ; Prognosis ; Pyridoxine ; therapeutic use ; Seizures ; diagnosis ; drug therapy ; genetics ; Status Epilepticus ; diagnosis ; drug therapy ; genetics

Objective To observe the time interval changes of the expression of Nogo-A in the intumescentia lumbalis of rats with experimental allergic encephalomyelitis(EAE),and explore the function of Nogo-A in the happening and development of EAE.Methods One hundred and six Wistar rats(female) were randomly divided into 2 groups:EAE group(70 rats)and control group(36 rats).Female Wistar rats were used to make EAE animal models,immunized by fresh cavia cobaya spinal cord homogenate and complete Freund adjuvant(CFA),at the same time,their blood brain barrier permeability was lifted by using pertussis stock solution.The rats those were coincident with the standard of being picked up were divided into 6 groups randomly according to the time of falling ill.In control group,fresh guinea pig spinal cord homogenate was replaced by sodium chloride,and rats were also divided into 6 groups randomly.Immunohistochemistry SP was used to detect expression of Nogo-A.Amyelination was detected by improved acid fuchsin and azulin myelin staining.Results In the control group,differences of Nogo-A expression in the intumescentia lumbalis were not obvious in each time group.In the EAE group,expression of Nogo-A could be observed in each time group,but their quantities were very different in contrast with those in the control group.One day after the rats had clinic symptoms,the expression of Nogo-A decreased to some extent;the third day,it reached the lowest point,and was significantly lower than that in control group(P

Objective To observe the efficacy and safety of joint mobilization therapy for osteoarthritis of knee joint.Methods 120 patients with osteoarthritis of knee joint were randomly divided into the treatment group and control group with 60 cases in each group. The treatment group were treated with joint mobilization and synthesis rehabilitation therapy, and control group only with synthesis rehabilitation therapy.Results The total effective rate was 90.00% in the treatment group and 76.67% in the control group according to analysis of intention-to-treat, and 92.73% in the treatment group and 80.77% in the control group based on analysis of per-protocol population. There was a significant difference between the two groups ( P<0.05). No adverse effect was found in the treatment group.Conclusion The joint mobilization therapy is efficacy and safety for osteoarthritis of knee joint.

China ; epidemiology ; Humans ; Pneumoconiosis ; epidemiology

The study is aimed to investigate the effect of plant growth regulators on yield and quality of the Salvia miltiorrhiza. The plant growth regulators was spraying on Salvia plants in July or August in field experiment, then the yield, ingredient content and the antioxidant activity were determined. The results showed that plant growth regulator 'Zhuanggenling' could increase the yield of Salvia with root-planting by 38.45%. Plant growth regulator 'Duoxiaozuo' could increase the yield of Salvia with seedling planting by 14.19%. Both plant growth regulator significantly reduced the antioxidant activity of Salvia in vitro, but they had no significant effect on active ingredient contents.
Diterpenes, Abietane ; analysis ; Phenanthrenes ; analysis ; Plant Extracts ; analysis ; Plant Growth Regulators ; pharmacology ; Salvia miltiorrhiza ; chemistry ; drug effects ; growth & development

Objective To investigate the genetic association between mutation in cytochrome P450 1A2 (CYP1A2) gene and Erxian suop response. Methods Forty-eight patients with male climacteric syndrome of both Yin and Yang deficiency received a treatment of erxian suop with three months. The severity of symptoms and responses to Erxian suop were quantizated by the method of Department of Psychology in Istanbul Bosphorus University and Clinical Research Guidance Principle on New Drug of Traditional Chinese Medicine published by China. A single nucleotide polymorphism (SNP) at position 2964 (G-A) of CYP1A2 gene were identified by polymerase chain reaction (PCR) in order to analyse the relationship between Erxian response and CYP1A2 single nucleotide polymorphism. Results Statistical analysis showed there was no significant difference among genetype and genetype distribution of CYP1A2 G2964A between the patients and control group. The genotype of the 16 patients among 32 effective patients was G/G (56.25%). The genotype of the 11 patients among 16 ineffective patients was G/A (68.75%). There was no significant difference in symptoms, physical sign between the two groups at the pre-treat and post-treat. Also the two groups had no significant difference among the score of major symptoms and hyposymptoms. Conclusion The patients with genotype of G/G have higher efficiency, while with genotype of G/A have lower efficiency among patients with CYP1A2 G2964A gene when treated with erxian soup.

Objective To establish the RP-HPLC method for determination of Bruchine and Strychine in Pingxiao Tablet. Methods The lichrospher C18 (4.6 mm?250 mm, 5 ?m) was Used as the stationary phase. The Mobile phase consisted of water-acetonitrile (60∶40) with 1.7 g sodium dodecylsulphate and 3.4 g KH2PO4 in 1 000 mL mixture. The Detecting wavelength was 254 nm and flow rate was 1.0 mL/min. The column temperature was 25 ℃. Result The linear ranage of Bruchine and Strychine were 0.102～0.918 ?g and 0.208 ～ 1.872 ?g respectively. The recovery rates of bruchine and strychnine were 101.07% (RSD=2.26%) and 99.29% (RSD=2.79%) respectively. Conclusion This method is realiable and accurate, the method can be applied to determination the contents of Bruchine and Strychine in Pingxiao Tablet.

Both levels of serum leptin and BMI were significantly higher in T2DM patients with severe NAFL (n=61) than those in T2DM with mild NAFL (n=24), T2DM without NAFL (n=45) and in normal control (n=30).