Objective To investigate the association between the results of Dermatophagoides pteronyssinus ( Der p) and Dermatophagoides farinae ( Der f) allergen SPT and serum sIgE in diagnosis of IgE-mediated mite allergy.Methods A total of 166 allergic outpatients with positive expression of serum sIgE of Der p and Der f diagnosed in Beijing Tongren Hospital,the General Hospital of Shenyang Military Region and Guangzhou Institute of Respiratory Disease were enrolled in the study.The SPT was performed by injecting liquid containing the allergen of Der p and Der f.One hundred ninety healthy subjects with negative serum sIgE of Der p and Der f were assigned as controls according to paired design ratio,1 to 1.The sensitivity and specificity of SPT used Der p and Der f allergen were evaluated in the diagnosis of mite allergy.The association between the results of SPT and serum sIgE was analyzed by linear regression method.Results In the allergic group,the positive rates of SPT for Der p and Der f were 98.80％ ( 164/166) and 95.18％ (158/166) respectively.In the control group,the positive rate of STP for Der p and Der f were 2.11％ (4/190) and 1.58％ (3/190) respectively.The SPT results of Der p and Der f showed significant statistical difference between healthy subjects and allergic patients (x2 =285.5,386.3;P＜0.01 ).The sensitivity and specificity of the SPT were 98.80％ (164/166) and 97.89％ (186/190) for Der p and 95.18％ (158/166) and 98.42％ (187/190) for Der f,respectively.There was a significant association between SPT and sIgE with linear correlation analysis( r =0.737,0.708;P ＜ 0.O1 ).Conclusions Both SPT of Der p and Der f is highly sensitive and specific.At the same time,SPT has a significant positive correlation with sIgE.It can be used as an accurate,convenient and fast test in diagnosis of mite allergic diseases.

Objective To explore the effects of sensory system obstacle on balance ability of patients with hemiplegia after stroke. Methods20 patients with hemiplegia after stroke (experimental group) and 20 normal people (control group) received Sensory Organization Test(SOT) with Smart Equitest Balance Master system. The balance index and the sensory index were accessed. Results The scores of the balanceindex in the experimental group were lower than in the control group in EC, SV, EOSS, ECSS, SVSS (P<0.05). The scores of somatosensory,visual and vestibular indexes in the experimental group were lower than in the control group (P<0.05), especially for vestibular index.Conclusion The sensory loss caused the balance dysfunction in patients with stroke.

ObjectiveTo study the effects and feasibility of Smart Equitest Balance Master training for severe balance disorder caused by cerebellar hemorrhage in children. MethodsA 10-year-old boy with severe ataxia caused by cerebellar hemorrhage were trained with Smart Equitest Balance Master. He was assessed with Smart Equitest Balance Master, Modified Barthel Index and Berg Balance Scale. ResultsAfter 22 weeks of balance training, he improved in equilibrium, gait, posture control, activity of daily living, dysarthria, and return to school to continue his study. ConclusionSmart Equitest Balance Master training can significantly improve balance function，motor function and activities of daily living after cerebellar hemorrhage.

Objective To study the effects of umbilical cord mesenchymal stem cells (UCMSCs) on the apoptosis of human breast cancer cell line MCF-7.Methods MCF-7 cells were co-cultured with different concentrations of UCMSCs.The apoptosis of MCF-7 cells was detected by in situ apoptosis and flow cytometry.Nude mouse subcutaneous tumor model was established by inoculating MCF-7 and MSCs cells subcutaneously on the right side of the back of a mouse.The MCF-7 cells were inoculated on the left side of the mouse as control.The tumor volume was measured every week to compare the difference between the two groups.On the 17th day after inoculation,the tumor tissue was harvested and the apoptosis of tumor cells was observed by a transmission electron microscopy.Results In situ apoptosis and flow cytometry showed that the early and late apoptosis rates of MCF-7 cells increased first and then decreased with the increase of UCMSCs concentration.The differences between the control and the MCF-7+UCMSCs group were statistically significant for early (F=39.80,P＜0.001) and late apoptosis rates (F=5.68,P＜0.01).The tumor volume of MCF-7+UCMSCs group was significantly lower than that of control group in 17 days after inoculation (F=9.81,P＜0.01).The representative apoptotic cells were observed by the transmission electron microscopyin the MCF-7 +UCMSCs group.Conclusion The UCMSCs with a certain concentration can effectively promote the apoptosis of MCF-7 cells.This study provides a certain experimental basis for the clinical treatment of breast cancer.

Objective To explore the effect of standing at a table while training on the upper limb function and muscle surface electromyography of hemiplegics.Methods Sixty hemiplegic persons were randomly divided into an experimental group and a control group,each of 30.The affected upper limbs of the experimental group were trained while standing at a table,while the control group was trained while sitting.Before the treatment,as well as after 2 and 4 weeks of treatment,both groups' motor functioning was evaluated using the Fugl-Mayer upper limb assessment (FMA),as well as muscle surface electromyography.Results Before the treatment there was no significant difference betweenthe two groups' average FMA scores.After 2 and 4 weeks of treatment it had increased significantly in the experimental group,but in the control group the increase was not significant until the fourth week.In terms of surface myography,significant differences were observed in the biceps femoris and gastrocnemius muscles of both groups after 2 weeks.Two weeks later there was further significant improvement in both groups except for the tibialis anterior muscles of the control group.The differences between the two groups were significant after two weeks in the electromyograms of the biceps femoris,gastrocnemius muscle and anterior tibialis.After four weeks the differences between the groups in all of the electromyograms were significant.Conclusion Compared with the traditional sitting position,standing at a table while training can effectively improve the muscle activity of the rectus abdominis and the spine so as to promote the recovery of movement in hemiplegic upper limbs.

BACKGROUND:Acellular vascular scaffolds can mimic the microstructure and function of native blood vessels,but some extracellular matrix loss occurs during their preparation,which affects their hemocompatibility.Therefore,it is necessary to modify them to improve their hemocompatibility. OBJECTIVE:To assess the hemocompatibility of acellular vascular scaffold prepared by Triton-x100/heparin sodium treatment. METHODS:The abdominal aorta was taken from SD rats and randomly divided into control and experimental groups.The control group was treated with Triton-x100 for 48 hours.The experimental group was treated with Triton-x100 for 48 hours and then treated with heparin sodium.The morphology and hydrophilicity of the two groups of acellular vascular scaffolds were detected.The hemocompatibility of the two groups of acellular vascular scaffold was evaluated by recalcification coagulation time test,platelet adhesion test,dynamic coagulation time test,hemolysis test,and complement activation test. RESULTS AND CONCLUSION:(1)Scanning electron microscopy showed that the surface of the two groups of vascular scaffolds was relatively intact,and a large number of fiber filaments appeared on the surface of the scaffolds after decellularity treatment,and the surface microstructure changed significantly.The water contact angle of the two groups of vascular scaffolds was smaller than that of natural vessels(P<0.000 1).There was no significant difference in water contact angle between the two groups(P>0.05).(2)The coagulation time of vascular scaffold was longer in the experimental group than in the control group(P<0.05).The number of platelets attached to the scaffold membrane was less in the experimental group than that in the control group(P<0.000 1).The coagulation index was greater in the experimental group than that in the control group(P<0.01),and the complement level was lower in the experimental group than that in the control group(P<0.001).The hemolysis rate of the two groups was lower than 5%of the national standard.(3)To conclude,acellular scaffold treated with Triton-x100/heparin sodium has excellent hemocompatibility.

Objective To evaluate the changes in the expression of hippocampal α7 nicotinic ace-tylcholine receptor (α7nAChR) , acetylcholinesterase ( AChE) and choline acetyltransferase ( ChAT) after sevoflurane anesthesia in neonatal rats. Methods Seventy-two healthy Sprague-Dawley rats of both sexes, aged 7 days, weighing 25-40 g, were divided into 3 groups ( n=24 each) using a random number table method: control group ( group C) , air and oxygen group ( group A∕O) and sevoflurane group ( group S) . Rats were exposed to carrier gas ( air 1 L∕min plus oxygen 1 L∕min) for 2 h in group A∕O. Rats were ex-posed to 3. 4％ sevoflurane in carrier gas for 2 h in group S. Eight rats in each group were selected at 2 h, 1 week and 4 weeks after the end of inhalation, and sacrificed, brains were removed and hippocampal tis-sues were obtained for determination of α7nAChR, AChE and ChAT protein and mRNA by Western blot and real-time polymerase chain reaction, respectively. Results Compared with group A∕O, the expression of α7nAChR mRNA was significantly down-regulated at each time point after the end of inhalation, and the expression of TnAChR was down-regulated at 2 h after the end of inhalation and up-regulated at 1 week after the end of inhalation, the expression of AChE mRNA was up-regulated at 2 h after the end of inhalation and down-regulated at 4 weeks after the end of inhalation, the expression of AChE was down-regulated at 4 weeks after the end of inhalation, the expression of ChAT mRNA was up-regulated at 2 h after the end of in-halation, and the expression of ChAT was down-regulated at each time point after the end of inhalation in group S ( P<0. 05) . Conclusion The expression of hippocampal α7nAChR is down-regulated at first and then up-regulated after sevoflurane anesthesia, the expression of ChAT and AchE in the later period is down-regulated, the tendency of protein expression mentioned above is different from that of its mRNA ex-pression, suggesting that sevoflurane may affect the protein expression through other pathways.