Adult ; Humans ; Liver Transplantation ; United States

Humans ; Liver Failure, Acute ; etiology ; therapy

Hepatorenal Syndrome ; diagnosis ; prevention & control ; therapy ; Humans ; Liver Transplantation

Objective To investigate the clinical features and treatment programs in children with Takayasu arteritis (TA) in order to improve awareness of the disease. Methods A retrospective study of hospi-talized children with TA in our hospital from Jan. 1999 to Dec. 2012 was performed. Results Between the 15 patients with TA, the ratio of male to female was 1∶2. The onset was from 14 months to 15 years old, with average age at (10 ±4) years old. It is according to (the European League against Rheumatism/the Pediatric Rheumatology European Society (EULAR/PRES) criteria for the diagnosis of TA. The most common clinical manifestations are hypertension, which occurred in 13 cases(87%), weak pulse or pulseless in 11 cases (73%), and heart failure in 10 cases (67%). About two-thirds of patients were diagnosed when the onset of heart failure occurred. The most common clinical type was type Ⅱ, which occrred in 9 cases (60%). Antihypertensive drugs, oral steroid and congestive heart failure controlling were the main treatment. Three patients with positive purifiedproteinderivative (PPD) test received anti-TB treatment. Three patients had vascular bypass surgery, one patient had percutaneous transluminal angioplasty. Conclusion TA may be life-threatening and progressive. Many patients are with advanced disease at the time of treatment, so the prognosis is generally poor. Early recognition is necessary to initiate appropriate therapy. The disease should be considered in patients with unexplained arterial hypertension or unexplained inflammatory syndromes without local signs.

Objective To study the cognition of scientific research integrity and know status of scientific research standard of nurses who had research experience so as to provide some evidence for nursing research integrity management.Methods A total of 296 nurses whose working years was more than 5years and who had delivered scientific research papers in 13 Grade A hospitals from 7 cities were investigated by self-designed questionnaires.Results More than 64.0％ nurses showed not appropriate or inappro-priate for those irresponsible research behavior.64.5％ nurses did not agree with the researchers to referring the research object situation for others,97.0％ nurses believed the research achievement should be objective.While nurses did not know the research standards well:only 56.1％ nurses knew the standard of submitting manuscript,and 20.6％ nurses knew research data collection,preservation and usage.Conclusions It is necessary to enhance the management of nursing research integrity through education and propaganda of the research standards,improving the scientific management system,increasing the research support for nurse scientific so as to promote the nursing research integrity.

Objective:To investigate the clinical efficacy of honeysuckle extraction liquid combined with nasal irrigation in the treatment of nasosinusitis after radiotherapy of nasopharyngeal carcinoma.Methods:Eighty-two patients with nasosinusitis post-radiotherapy who received treatment between February 2018 and February 2020 in the First Hospital of Jiaxing were included in this study. They were randomly assigned to receive either nasal irrigation with 0.9% sodium chloride injection (control group, n = 41) or atomization treatment with honeysuckle extract liquid plus nasal irrigation with 0.9% sodium chloride injection (observation group, n = 41), once daily for 4 successive weeks. Visual Analogue Scale (VAS) and nasal endoscopic Lund-Kennedy (LK) score, ciliary transport rate, clinical efficacy and adverse reactions were compared between the control and observation groups. Results:After treatment, the nasal congestion score [(0.82 ± 0.10) points], viscous or purulent nasal discharge score [(1.88 ± 0.25) points], olfactory dysfunction score [(1.12 ± 0.15) points], head and face pain score [(0.40 ± 0.06) points] and nasal endoscopic Lund-Kennedy score [(2.03 ± 0.26) points] in the observation group were significantly lower than those in the control group [(1.94 ± 0.26) points, (2.93 ± 0.37) points, (1.73 ± 0.25) points, (0.62 ± 0.09) points, (4.07 ± 0.47) points, respectively, t = 9.156, 15.056, 13.397, 13.023, 9.156, all P < 0.01]. The ciliary transport rate in the observation group [(4.71 ± 0.56) mm/min] was significantly higher than that in the control group [(3.94 ± 0.51) mm/min, t = 15.056, P < 0.01]. Total effective rate in the observation group was 97.56% (40/41), which was significantly higher than 80.49% (33/41) in the control group ( χ2 = 4.493, P < 0.05). In the control group, one patient had epistaxis, and no adverse reaction was observed in the observation group. Conclusion:Honeysuckle extraction liquid combined with nasal irrigation with 0.9% sodium chloride injection is effective in the treatment of nasosinusitis after radiotherapy for nasopharyngeal carcinoma, can greatly improve the symptoms and signs of patients, and is highly safe.

Objective: Large-conductance calcium-activated potassium (BKCa) channel modulates vascular smooth muscle tone. In the present study, we tested the hypothesis that salt, one of the factors which significantly influence blood pressure (BP), can regulate BKCa activity and then elevate blood pressure. Methods: Male Sprague-Dawley rats aged 6 weeks were randomized into high salt diet group (HS) and control group, fed with high salt diet (containing 5% NaCl) and standard rat chow (containing 0.4% NaCl) respectively for 16 weeks. Tail systolic blood pressure (SBP), body weight (BW) and 24-hour urinary output were tested every 4 weeks. Content of urinary Na+ was detected using flame spectrophotometrical method. At the end of 16 weeks, all the rats were killed, the mesenteric arteries were obtained, and single mesenteric smooth muscle cells were isolated at once. The resting membrane potential (Em), the total potassium currents and the currents after perfusion with TEA solution of the cells were all recorded by whole cell patch clamp. The transcriptions of BKCa channel α and β1 subunits in mesenteric arterial vascular smooth muscle cells (VSMC) of each group were calculated by real-time RT-PCR. Results: There was no difference in SBP and BW at each stage between control group and HS group; the urinary Na+ level in HS animals was elevated significantly after 4 weeks. The negative values of Em in HS group VSMCs were reduced compared with those in the control group. Transcriptions of β1 subunit of BKCa channels were decreased in HS group, but α subunit transcriptions did not differ between the two groups. Whole cell potassium currents did not differ between HS and control groups, but BK Ca currents of HS group VSMCs were lower than those of control group ones. Conclusion: Even without elevating SBP, salt-loading can still modulate the expression and activity of BKCa channel in the mesenteric arterial VSMC and elevate vascular tone.

Objective: Large-conductance calcium-activated potassium (BKCa) channel modulates vascular smooth muscle tone. In the present study, we tested the hypothesis that salt, one of the factors which significantly influence blood pressure (BP), can regulate BKCa activity and then elevate blood pressure. Methods: Male Sprague-Dawley rats aged 6 weeks were randomized into high salt diet group (HS) and control group, fed with high salt diet (containing 5% NaCl) and standard rat chow (containing 0.4% NaCl) respectively for 16 weeks. Tail systolic blood pressure (SBP), body weight (BW) and 24-hour urinary output were tested every 4 weeks. Content of urinary Na+ was detected using flame spectrophotometrical method. At the end of 16 weeks, all the rats were killed, the mesenteric arteries were obtained, and single mesenteric smooth muscle cells were isolated at once. The resting membrane potential (Em), the total potassium currents and the currents after perfusion with TEA solution of the cells were all recorded by whole cell patch clamp. The transcriptions of BKCa channel α and β1 subunits in mesenteric arterial vascular smooth muscle cells (VSMC) of each group were calculated by real-time RT-PCR. Results: There was no difference in SBP and BW at each stage between control group and HS group; the urinary Na+ level in HS animals was elevated significantly after 4 weeks. The negative values of Em in HS group VSMCs were reduced compared with those in the control group. Transcriptions of β1 subunit of BKCa channels were decreased in HS group, but α subunit transcriptions did not differ between the two groups. Whole cell potassium currents did not differ between HS and control groups, but BK Ca currents of HS group VSMCs were lower than those of control group ones. Conclusion: Even without elevating SBP, salt-loading can still modulate the expression and activity of BKCa channel in the mesenteric arterial VSMC and elevate vascular tone.

Objective To explore the most suitable condition of influenza virus which was culturing on plateau region,and im-prove the effect of the separation of influenza virus.Methods The original specimens were respective inoculated in MDCK cells,by comparing CO2 percent concentration (5%,4.8%,4.6%,4.4%,4.2%),TPCK-pancreatic enzyme dosage (1,2,3, 4,5μg/m and 6,8,10μg/ml),inoculation amount (100,200,300,400,500μl),incubation time (48,72,96,120,144 h),inoc-ulation methods (adsorption,direct inoculated),and culture vessel(cells,6 orifice)that were influenced on influenza virus, the best culture conditions was determined.Hemagglutinatio (HA)method was used to detect the virus titers.Results Through the comparison,the HA titer of virus was in the highest titer with 6 orifice plate culture vessel,4.4% CO2 and 4.6% CO2 ,2μg/ml TPCK-pancreatic enzyme,300μl and 400μl inoculation amount,72h~96h of incubation time.Conclusion The optimal condition of the influenza virus cultured with the MDCK cell has been established in the laboratory on plateau region.

Objective To investigate the pathogenesis of hyperthyroidism combined with cerebral infarction and the relationship between hyperthyroidism and cerebral infarction. Methods Data from the clinical manifestations, diagnosis, and therapeutic outcomes of 10 patients with hyperthyroidism combined with cerebral infarction were analyzed retrospectively. Results After treatment with drugs for anti hyperthyroidism and active treatment of cerebral infarction, no severe complications were found in all patients. Conclusion Hyperthyroidism combined with cerebral infarction may be one of the important causes of adolescent cerebral infarction. Treatment of hyperthyroidism may play an important role in the treatment of cerebral infarction.