Objective To investigate the distribution and synaptic connectivity of neuropeptide Y (NPY)-like immunoreactivity (IR) in Bullfrog retina, Rana Calesbeiana, especially in the inner plexiform layer. Methods Light microscopy immunofluorescence and post-embedding colloidal gold labeling electron microscopy techniques were employed in this study. Results NPY immunoreactivity was associated with a small population of amacrine cell bodies in the inner nuclear layer and cell processes, ramified with 3 distinct bands, in the inner plexiform layer. As for ultrastructure, quantitative analysis showed that NPY-IR amacrine cell processes were most often presynaptic to NPY negative amacrine cell processes (49.7%) and ganglion cell dendrites (49.3%). NPY-IR amacrine cells predominantly received synaptic input from bipolar cell axon terminals (86%), while a few input from other amacrine cell processes (14%). Conclusion NPY-IR in Bullfrog retina is mainly distributed in the inner part and predominately receives excitatory glutamate modulation from bipolar cells.

Objective To analyze the clinical efficacy and safety of laparoscopic and open uterine myomectomy.Methods 160 cases of uterine fibroids diagnosed by our hospital from April 2012 to April 2016 were selected as study cases.The patients were divided into two groups according to the order of treatment.80 patients in the control group received open uterine myoma rejection.80 patients in the observation group received laparoscopic myomectomy.The clinical treatment effect and complication were analyzed.Results The operation time,amount of intraoperative blood loss and the incision size of the observation group were (78.46 ± 10.31)min,(86.24 ±7.65) mL,(25.36 ± 1.28) mm,respectively,which were significantly less than those in the control group,the differences were statistically significant (t =6.57,6.81,7.66,all P ＜ 0.05).The time of postoperative body temperature recovery,time of anal exhaust,time of bedtime and the hospitalization time of the observation group were (24.16 ±3.81)h,(18.29 ± 4.36)h,(11.38± 2.06)h,(4.63 ± 1.51)d,respectively,which were significantly shorter than those in the control group (t =6.25,7.38,7.70,6.27,all P ＜ 0.05).The operative rate and postoperative analgesia rate of the observation group were 6.25％ and 11.25％,respectively,which were significantly lower than those of the control group (x2 =8.33,9.07,all P ＜ 0.05).The incidence rate of postoperative complications was 6.25％ in the observation group,which was significantly lower than 25.00％ in the control group,the difference was statistically significant (x2 =8.65,P ＜ 0.05).Conclusion Laparoscopic myomectomy has the advantages of less trauma,less bleeding,quick recovery and less complication,and high safety.It is the ideal technique for the treatment of uterine fibroids,which is worthy of promotion and application.

OBJECTIVE To investigate the feature of nosocomial infections of our hospital inpatients,and analyze the correlative factors,based on which effective methods are taken.METHODS An analysis was made on the basis of the survey,the data came from 58 324 hospitalized cases from 2003 to 2006.RESULTS Of them 2 509 cases were infected,the incidence was 4.30%.The lower respiratory tract ranked the first place (26.66%)and the highest infected rate was in Hematology Department(7.79%).The species were predominated by Gram-negatives(58.04%).CONCLUSIONS Strengthening the nosocomial infection management,the infection rate could be decreased after taking prevention measures according to various correlative factors.

Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Fatty Liver ; drug therapy ; Humans ; Phytotherapy

Burns ; complications ; Humans ; Multiple Organ Failure ; etiology ; prevention & control

One biocontrol bacillus B3 was isolated from peanut rhizosphere, which showed a broad inhibition spectrum against various soil-borne plant phytopathogenic fungi. It was also found to be capable of utilizing beta-cypermethrin, chlorpyrifos and imidacloprid as the sole source of carbon for growth. The extent of degradation of them at initial concentration of 100 mg/L was 54.42%, 57.44% and 49.24% respectively within 7 days. Strain B3 was identified as Paenibacillus polymyxa based on morphological, physiological-biochemical properties, and 16S rRNA sequence analysis. This study indicated that strain B3 had potential application in biological control and soil bioremediation.

Objective: To ascertain the condition for extracting tetramethylpyrazine from Rhizoma Chuanxiong with alcohol. Methods: With the transferring rate of tetramethylpyrazine as index, the optimum extraction condition for the first time was selected by uniform test, and the times of extraction was studied. Results: Taking 80% alcohol as solvent, Rhizoma Chuanxiong was extracted two times with 8 and 6 times volume of alcohol for 2hr and 1.5hr, respectively. In this condition, tetramethylpyrazine could be effectively extracted. Conclusion: The experimental results provided the basis for the ascertainment of extraction process of Rhizoma Chuanxiong preparation.

Objective:To observe influence of preinfarction angina pectoris (PAP)on primary ST elevation acute my-ocardial infarction (AMI).Methods:A total of 280 patients with primary ST elevation AMI received coronary angi-ography (CAG)within 24h after symptoms occurred,and they received ECG and QRS scoring on hospitalization. According to PAP or not,patients were divided into PAP group (n=102)and non PAP group (n=178).According to attack to CAG time,patients were divided into early stage group (<2h,n=60),mid-term group (2~6h,n=150)and advanced stage group (6~24h,n=70).Results:QRS score of PAP patients [(2.4±2.4)scores]was sig-nificantly lower than that of non PAP patients [(3.2±3.0)scores],percentage of high QRS score in PAP group (8.0%)was also significantly lower than that of non PAP group (18.4%),P<0.05 both.In early stage group, there was no significant difference in QRS score [(2.0±1.8)scores vs.(2.6±2.8)scores,P=0.35]between PAP and non PAP patients;The QRS score of PAP patients [(2.0±2.1)scores]was significantly lower than that of non PAP group [(3.0 ±3.0)scores]in mid-term group,P=0.03;in advanced stage group,the QRS score all signifi-cantly rose in PAP patients [(4.1 ±3.3)scores]and non PAP patients [(5.5±2.9)scores],P=0.13;QRS score gradually rose along with onset time prolonged in non PAP group.Conclusion:Preinfarction angina pectoris can de-lay progress of acute myocardial infarction and expand therapeutic window of reperfusion.

Objective] Summarize the pathogenesis of asthma and leukotriene receptor in latest research situation. [Methods]Check out the past five years CNKI, VIP data, PUBMED data to leukotrienes and asthma for keyword research literature, and classification, summary. [Results]Numerous clinical studies, in vivo and in vitro experiments showed that leukotrienes and their receptors are involved in the pathogenesis of asthma and progress in a multi-cell, multi-session, and interact with a variety of cytokines.[Conclusion]With concept of wholism as the characteristics of the Genomics technologies, more suitable as a primary means of application in the field of medical research has an overall concept for the regulation of traditional Chinese medicine research in the field of drugs to treat asthma leukotriene pathway and provide a new perspective.

Version 2015 of the National Comprehensive Cancer Network (NCCN) Guidelines for Uterine Neoplasms uses the 2009 International Federation of Gynecology and Obstetrics (FIGO) staging system.These NCCN guidelines divide pure endnmetrioid cancers into three categories to delineate treatment:(1) disease limited to the uterus;(2) cervical involvement;(3) extrauterinc disease.Surgery is recommended for medically operable patients.Continuous progestin-based therapy may be considered for highly selected patients with stage IA disease who wish to preserve their fertility.Preoperative chemotherapy can be considered if extrauterine disease is suspected.Factors that may influence the decision regarding adjuvant therapy include lymph vascular space invasion (LVSI),patient age,tumor volume,and lower uterine segment or surface cervical glandular involvement.When administering adjuvant radiotherapy,it should be initiated as soon as the vaginal cuff has healed,no later than 12 weeks after surgery.