Issues of Chinese medical health reform are analyzed and approached associated with the US federal medical health system. We made the suggestions that establish the public health service system covering both urban and country residents, push on the reform of medical insurance, drug circulation and medical system. We advocate that implement the scientific development view and hold the opportunity, impel the medical health reform in general.

Angiogenesis is one of the important pathological characteristics in the development of tumor growth. Hence ,an?ti-angiogenes is has become a hot topic in the field of cancer research. The current strategy for anti-angiogenesis therapy is to restore the angiogenic balance which is broken in the tumor via either block of proangiogenic factorsor application of angiogenic inhibitors. Endogenous angiogenic inhibitors show more promising prospects compared with proangiogenic factor antagonists. However ,the un?derlying mechanisms for the angiogenic inhibitors remain to be thoroughly elucidated. There are two kinds of endogenous angiogenic inhibitors,one is the hydrolyzed fragments of precursor protein,such as plasminogen Kringle 5(K5),angiostatin/kringle 1~4,end?ostatin,etc;the other is cell secreted proteins,such as pigment epithelium-derived factor(PEDF),kallikrein-binding protein (KBP/kallistatin),antithrombin,etc. Here we summarized the research progresses on the biological functions,underlying mecha?nisms of tumor angiogenesis and application prospects of K5,PEDF,and KBP,so as to provide insights into the antiangiogenic ther?apies of tumor in the future.

Cognitive behavior therapy (CBT) has been proved to be an effective psychological treatment method for depression,especially for mild-to-moderate depression,however,due to the influence of various factors,its application in patients with depression is limited.Studies abroad have confirmed self-help intervention based on CBT has good effects on patients with depression.By reviewing efficacy of self-help interventions based on CBT on depression,it can be known that most of the literatures support that self-help interventions based on CBT has good effects on depression.Related researches abroad have been more mature,but few similar studies have been conducted in our country.So self-help CBT for depression has broad prospects in our country,which can play an important role in the prevention and therapy of depression,alleviating the shortage of medical resources,and reducing the patients' medical expenses,etc.Based on bibliotherapy (a major form of self-help interventions based on CBT),future studies need to learn from foreign practice to expand the audience to the clinic,community and non-clinical patients,in order to provide advice for interventions for depression in China.

Objective:To explore the effect of Shenfu Injection (SFI) on expression of Heme oxygenase-1 (HO-1) and iNOS (inducible nitric oxide synthase) in renal failure rats induced by intestinal ischemia-reperfusion (IR) and its possible mechanism. Methods:The model of intestinal ischemia-reperfusion was induced by clamping superior mesenteric artery for one hour and then releasing the arterial clamp for six hours. Wistar rats were randomized into three groups:IR+normal saline group,IR+SFI group and control group (C group). The serum creatinine and blood urea nitrogen were observed respectively. Expression of HO-1 and iNOS in rat kidney tissue was detected by immunohistochemitry and morphometry computer image analysis. The histological change of kidney was observed under light microscope. Results:①Compared with C group,expression of HO-1 and iNOS increased markedly in IR+ normal saline group (P

Carbon nantubes composites draws more and more attention these days. Its application provides new potential for the reinforcement and toughness of dental ceramic. In this paper, the properties of carbon nanotube, carbon nanotube based ceramic composites and its mechanics of toughness, and the future trend of carbon nanotube in dental ceramic material, have been reviewed.

Objective To prepare monoclonal antibody (mAb) against recombinant human cardiac troponin I(cTnI).Methods The full-length gene encoding human cardiac troponin I (cTnI) was synthesized chemically and inserted into expression plasmid pBV220 to construct recombinant plasmid p pBV220/cTnI. The recombinant plasmid was transformed into E.coli DH5? which then expressed cTnI. The immunological activity of the expressed cTnI was analyzed by Western blot. Recombinant human cTnI protein was used as antigen to immunize BALB/c mice. Monoclonal anti-bodies against cTnI were prepared by normal hybridoma technology. The relative affinity of mAbs was determined by ELISA. Specificity of mAbs was analyzed by Western blot.Results Human cTnI gene was synthesized and confirmed by DNA sequencing. Positive recombinant clones were identified by restriction enzyme digestion analysis and DNA sequencing. Western blot analysis showed that the cTnI protein could be recognized by an anti- cTnI antibody. Two hybridmas producing antibodies against cTnI were obtained. IgG isotypes of two mAbs were IgG2a and IgG2b. Western blot showed that the antibodies were specific for cTnI. Neutralisation test showed that these mAbs could be evidently neutralized by cTnI.Conclusion The recombinant expression plasmid of cTnI was constructed successfully and expressed in E.coli. The method of EL ISA established to test serum cTnI is to clinically useful. The cTnI mAb which using cTnI as antigen prepared in this paper can be used for cTnI immunoassay in vitro.

A MSP430-based detection project of blood pressure (BP) is put forward, in which the application of the portable monitor is taken into considerations. High- precision sensors and parameter recognition are also involved in. BP parameter extraction and real-time control of the system are discussed in detail.

Objective To study the effect of anti-hypertensive therapy on the characteristics of platelet in essential hypertension(EH) patients. Methods Thirty-two healthy people(CT) and 37 patients with primary hypertension (gradeⅠ) were recruited . Blood pressure and 4 platelet parameters: platelet count (PLT), mean platelet volume (MPV), platelet distribution width (PDW) and platelet hematocrit (PCT) were determined by whole blood automatic analysis apparatus. Results The 4 platelet parameters were increased in hypertension patients compared with healthy people and decreased after anti-hypertensive therapy[PLT:(before 201.8?72.9 vs after 187.1?68.6 )10~ 9 L+{-1}; MPV:(before 10.3?1.9 vs after 10.0?1.9)fL, PDW:(before 15.5?1.1 vs after 15.2?1.0)fL; PCT:(before 0.198%?0.047% vs after 0.187%?0.036%)](P

OBJECTIVE To investigate the resistance of Staphylococcus to erythromycin and clindamycin and detect the percentage and gene for inducible resistance in Tai'an.METHODS The susceptibilities of Staphylococcus to erythromycin and clindamycin were examined by Kirby-Bauer disc agar diffusion test and the inducible erythromycin resistance to clindamycin was checked by D-test according to the standards of NCCLS,and the resistance genes msrA,Vgb,sat4,ermA,ermB and ermC were detected by using PCR technology.RESULTS Among the 326 strains,162(44.12%)were all resistant to erythromycin and clindamycin;68(20.86%)were resistant to erythromycin and sensitive to clindamycin but they were positive in D-test;42(12.88%)were resistant to erythromycin and sensitive to clindamycin but they were negative in D-test.The rates of inducible resistance of MRSA,MSSA,MRCNS and MSCNS to clindamycin were 40.00%,56.25%,63.38% and 66.67%,respectively among the Staphylococcus which were resistant to erythromycin and sensitive to clindamycin.The gene ermC was the main one for inducible erythromycin resistance to clindamycin.The percentage of gene ermC was 85.29% and that of ermC and sat4 either was 7.35%;all the others were negative.CONCLUSIONS The rate of inducible erythromycin resistance to clindamycin in our area is relatively high,and D-test in clinical microbiology laboratory should be done so that the physicians can select the reasonable MLSB antimicrobial agents.

The effect of artrial natriuretic polypeptides on the intracellular free. calcium concentration of immune cells of spontaneously hypertensive rats has been investigated. It was found that artrial natriuretic polypeptides could raise directly the intracellular free calcium concentration of thymocytes, splenocytes and lymphonode cell, or cooperate with Con A in increasing the intracellular free calcium concentration of rat lymphocytes and T lymphocytes except for B cells. It was also found that artrial natriuretic polypeptides could cooperate with IL-2 in increasing the intracellular free calcium concentration of rat lymphoblast induced by Con A. The extent of increased intracellular free calcium concentration induced by artrial natriuretic polypeptides in immune cells of spontaneously hypertensive rats was lower than that of WKA rats.