Purpose:Evaluate the prognostic factors in invasive vulvar cancer.Methods:Fifty one cases with invasive vulvar cancer admitted in our hospital from 1980 to 1999 were retrospectively analyzed.Results:Thirty six patients had squamous cell carcinoma and 27 of them were well differentiated and 6 moderately differentiated and 3 poorly differentiated ,three had adenocarcinoma, three had basal cell carcinoma ,three had leimyosarcoma , six had malignant melanoma. Thirteen cases (25.4%) were in FIGO stage Ⅰ;29 (56.7%) in stage Ⅱ;7(13.1%) in stage Ⅲ and 2(3.9%) in stage Ⅳ. The 5-year survival rates of stage Ⅰ,Ⅱ,ⅢandⅣwere 81.5% , 68.4% , 41.2% and 0.0% (P

Objective:To construct a EGFP(Enhanced green fluorescent protein)-labled euk-aryotic expression plasmid of Fcy::Fur suicide gene and to detect its expression in SKOV3 cell line. Methods:With the technology of gene re-arrangement,Fcy::Fur gene in pORF-Fcy:Fur plasmid was subcloned into pEGFP-N1 vector,with its correctness evaluated by the means of r-estriction enzyme analysis and sequencing.It was transfected into SKOV3 cells with lipofectin,the transient expression of GFP was observed under flu- orescence microscope after 24 hours and detected by Western blot. Results:Correct construction of pEGFP-N1-Fcy::Fur was identi- fied by methods of restriction enzyme analysis and nucleotide sequence determination.A total of 60% transfe-cted cells emitted out green fluorescence under fluorescent microscope after 24 h after transfecti-.on. Fcy::Fur gene expressed by the transfected cells were testified by Western blot. Conclusion:The recombinant eukaryotic expression vectors have been constructed successfully and effec- tive-ly expressed in ovarian cancer cells,which may provide an experimental basis for gene therapy of ovarian cancer.

Abdomen ; surgery ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Combined Modality Therapy ; Female ; Humans ; Lipid Mobilization ; Male ; Middle Aged ; Pain, Postoperative ; metabolism ; therapy ; Postoperative Period ; Short-Wave Therapy ; Young Adult

Objective To investigate the clinical effect of cefoperazone sodium and sulbactam sodium. Methods 56 cases who used cefoperazone sodium and sulbactam sodium for anti -infection were selected as the observation group,and 56 hospitalized patients who used cephalosporin ceftazidime were selected as the control group. The observation group was intravenously injectioned cefoperazone sodium and sulbactam sodium,the control group received intravenous ceftazidime.The clinical efficacy and adverse reactions of the two groups were compared.Results After treatment,the cure rate and total effective rate of the observation group were 55.36%,98.21%,which of the control group were 35.71%,91.07%.The cure rate of the observation group was significantly higher than that of the control group (χ2 =4.356,P <0.05),and the total effective rate between the two groups had no statistically signifi-cant difference (χ2 =2.817,P >0.05).The incidence rate of adverse reactions of the two groups had no significant difference.Conclusion Cefoperazone sodium and sulbactam sodium therapy has good clinical efficacy in the treat-ment of infectious diseases,and has low incidence of adverse reactions,good safety,which can be popularized and applied in clinic.

With the deepening of the lung cancer molecular biology research,small molecular targets antitumor drugs make breakthrough progress,the epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI) is one of the most attention drug.A series studies show that EGFR-TKI can enhance the antitumor activity of ionizing radiation.Therefore,EGFR-TKI combined with radiotherapy alone for poor-risk patients appears survival benefit,but can't ignore the lung toxicity.However,there is a big contro-versy that EGFR-TKI combined with chemoradiotherapy for locally advanced NSCLC.

Epstein-Barr Virus (EBV) is the first human virus related to oncogenesis.EBV infection is associated with inflammatory bowel disease (IBD) with unknown causality.The prevalence of EBV in intestinal tissue from patients with IBD is significantly higher and related to the exacerbation of the disease and refractory IBD.Immunosuppressive therapy has improved outcomes associated with IBD.However,it is also associated with an increased risk of opportunistic infection,and lymphoproliferative disorders (LDs) maybe due to EBV infection.Here we review our current understanding of the pathogenesis of EBV infection in colonic mucosal inflammation,EBV-induced disease exacerbation,lymphomagenesis in IBD,and clinical approaches therefrom.

Objective To compare the clinical effect and safety of two kinds of quadruple therapy on the basis of omeprazole and rabeprazole in the treatment of patients with active gastric ulcer and Hp positive.Methods 104 patients with active gastric ulcer and Hp positive were chosen,and they were randomly divided into two groups including A group (52 patients)with omeprazole treatment,and B group (52 patients)with rabeprazole treatment on the basis of amoxicillin +clarithromycin +bismuth potassium citrate.The clinical efficacy,clinical symptom remission rate in 7d,14d and 28d after treatment,HP eradication rate,recurrence rate with follow -up and adverse reaction inci-dence of 2 groups were compared.Results The clinical cure rate of B group was significantly higher than A group (36.54% vs.19.23%)(χ2 =8.74,P <0.05).There was no significant difference in the clinical total effective rate between the two groups(P <0.05).The clinical symptom remission rates in 7d and 14d after treatment of B group were significantly higher than A group(96.15% vs.76.92%,96.15% vs.78.85%,98.08% vs.82.69%;98.08%vs.84.62%,100.00% vs.82.69%,100.00% vs.88.46%)(χ2 =8.74,7.20,7.91;7.05,6.86,6.33;all P <0.055).The Hp eradication rate of B group was significantly higher than A group(92.31% vs.73.07%)(χ2 =9.24,P <0.05).The recurrence rate of B group was significantly lower than A group(7.69% vs.25.00%)(χ2 =10.62,P <0.05).The incidence rate of adverse reaction of B group was significantly lower than A group(3.85% vs. 13.46%)(χ2 =7.85,P <0.05).Conclusion Compared with omeprazole,quadruple therapy on the basis of rabe-prazole in the treatment of patients with active gastric ulcer and Hp positive can effectively relieve the digestive symp-toms,promote ulcer repair process,higher the Hp removal effects,prevent the long -term recurrence and is helpful to reduce the adverse drug reactions risk.

Objective To study the clinical effect of small incision cataract extraction in the treatment of acute angle-closure glaucoma with cataract.Methods Fifty-eight patients with acute angle-closure glaucoma combined with cataract were selected,and according to the digital table they were randomly divided into two groups:control group and observation group.The patients in the control group were treated by conventional method.The patients in the observation group were treated with small incision cataract.The changes of intraocular pressure,anterior chamber depth and visual acuity before and after treatment were observed.The incidence of complications and the satisfaction of treatment were compared between the two groups.Results There were no significant differences in IOP,visual acuity and anterior chamber depth between the two groups before treatment(all P>0.05).After treatment,the intraocular pressure,anterior chamber depth and visual acuity of the observation group were (10.2±4.0)mmHg,(3.3±0.5)mm,(0.85±0.08),respectively,which of the control group were (17.5±5.0)mmHg,(3.2±0.5)mm and (0.50±0.06),respectively.The improvement of intraocular pressure and anterior chamber depth of the observation group was more significant than the control group(t=0.120,0.520,1.012,all P<0.05).The satisfaction rate of the observation group(96.6%) was higher than that of the control group(75.8%)(χ2=9.823,P<0.05).There was no significant difference in the incidence of complications between the two groups(P>0.05).Conclusion Small incision cataract extraction is an effective treatment for acute angle-closure glaucoma complicated with cataract,and the postoperative complications are less.The patients are satisfied with the treatment and deserve to be popularized.

[Objective] To observe the clinical effect of Jiedu Qinghuo Drink on children infection mononucleosis(IM). [Method] Randomly divide 54 cases into treatment group 27 cases and control one 27 cases. The control one was given Ganciclovir, the treatment one added with Jiedu Qinghuo Drink. [Result] The total effective rate was 85.19% for treatment group, and 55.56% for control one, the comparison of both had difference. [Conclusion] Jiedu Qinghuo Drink has good cure effect on children IM.

Immune factor is one of the main mechanism of children glomerular diseases. Tacrolimus has a strong and effective immunosuppressive action comparing with CsA. In recent years,the curative effect of ta-crolimus in the treatment of children with kidney disease has attracted doctors attention. Tacrolimus in the treat-ment for refractory nephrotic syndrome ( steroid-dependent nephrotic syndrome, steroid-resistent nephrotic syn-drome and the frequent recurrence of glomerular syndrome) has obtained the good effect. This article reviews the application of tacrolimus in nephrotic syndrome,focal segmental glomerular sclerosis,mesangial proliferative glo-merulonephritis,membranous nephropathy,lupus nephritis( LN) in the children with kidney disease.