Aim To investigate the effects of magnesium lithospermate B(MLB) on vasoconstriction of denuded aortic rings in vitro,as well as the effects of MLB on cytosolic free calcium concentration([Ca~(2+)]_i) in aortic vascular smooth muscle cells(AVSMCs).Methods Isotonic tension was measured in the rat thoracic aortic rings without endothelium and quantitative changes of [Ca~(2+)]_i were directly monitored in AVSMCs loaded with the fluorescent calcium indicator Fluo-3 using intracellular cation measurement system.Results MLB had no effects on resting ring tension in the presence or absence of extracellular Ca~(2+).In rings exposed to 50～200 ?mol?L~(-1) MLB,the isotonic contractions induced by 1 ?mol?L~(-1) PE were decreased in calcium-free K-H solution.However,in the presence of extracellular Ca~(2+),KCl 60 mmol?L~(-1) produced isotonic contractions that were abolished by verapamil 10 ?mol?L~(-1) or inhibited by pretreatment of 50～200 ?mol?L~(-1) MLB in a concentration-dependent manner.When administered to the rings precontracted with 1 ?mol?L~(-1) PE in the absence of extracellular Ca~(2+),a second extracelluar-calcium-dependent vasoconstriction induced by restoration of extracellular Ca~(2+) was also reduced by pretreatment of 50～200 ?mol?L~(-1) MLB.Moreover,MLB,at concentration of 50～200 ?mol?L~(-1),produced little effect on AVSMCs [Ca~(2+)]_i in the presence or absence of extracellular Ca~(2+).In AVSMCs exposed to 50～200 ?mol?L~(-1) MLB,the inhibitory effects of MLB on [Ca~(2+)]_i induced by 20 ?mol?L~(-1) ATP were concentration-dependent,with decreases ranging from 17.4% to 61.1% in the absence of extracellular Ca~(2+).In the presence of extracellular Ca~(2+) and thapsigargin,the increases in AVSMCs [Ca~(2+)]_i evoked by 60 mmol?L~(-1) KCl were inhibited by pretreatment with MLB(50～200 ?mol?L~(-1)) or abolished by pretreatment with verapamil(10 ?mol?L~(-1)).Conclusions MLB inhibits vasoconstriction induced by PE,high-K~+,and re-Ca~(2+).MLB also has inhibitory effects on increase of [Ca~(2+)]_i induced by ATP and high K~+ in AVSMCs.These results indicate that both the blocking effects on calcium release and voltage-dependent calcium channels may be responsible for the effects of MLB on [Ca~(2+)]_i in vascular smooth muscle cells.

Objective To investigate the microcirculative route of the arterialized venous flap.Methods Selecting 15 Chinese white rabbits designed the model of arterialized venous flap by anastomosis with from the central artery to central vein.The reversed blood flow perfusion was directly observed by the "ear windows" under the microscope and,simultaneous recording the changes in the microcirculation of the flap.Results All of the flaps were alived.The structure of the capillary net in the flap was a stereo-net .In the early arterialized stage,the upstream blood flows quickly in the venule;there was no blood flowing in the capillary net with the opening of the capillary net,the reconstructed procedure of the non-physilogical microcirculation was accomplished:the arterial blood mainly flow through small vein and venule,finally arrived the capillary net.Conclusions Keep up the drainage vein free,the arterialized venousflap could be alived by reconstructed effective microcirculation through the venous system.

Objective To investigate expressions of albumin during retinal neovascularization in a murine model of oxygen -in-duced retinopathy.Methods C57BL/6J mice were exposed to (75 ±2)%oxygen for 5 days and returned to room air to induce retinal neovascularization .Retinal neovascularization was observed by fluorescence angiography , and was quantified by counting the endotheli-al nuclei protruding into the vitreous cavity after hematoxylin-eosin staining.The blood-retina barrier was evaluated by Western blot a-nalysis of extravasated albumin in the retina .Results In fluorescence angiograms , irregular neovascularization and fluorescein leakage was observed surrounding the unperfused areas in the hypoxic group .Compared to the normoxic group , there was a significant increase in both-side retinal albumin protein levels in the hypoxic group at P 13, P15 , and P17.Conclusions Albumin expression was in-creased in murine retina under hypoxia , and might be used to detect blood-retinal barrier damage of oxygen-induced retinopathy .

The peritoneum is one of the most common sites of gastric cancer recurrence or metastasis.Peritoneal metastasis seriously affects the patients life quality and shortens patients survival time,so early diagnosis and active treatment have a major impact on the prognosis. Continuous studies of some biomarkers and extensive using of ncw imaging technology make the early diagnosis of peritoneal metastasis more promising.Simultaneously,with the rapid development of intraperitoneal chemotherapy and molecular targeted therapy,there are more options in the treatment.

The clinical data of 25 patients with acute exacerbations of chronic obstruction pulmonary disease (AECOPD) admitted from March 2009 to October 2010 were analyzed. The sputum samples were collected and the levels of inflammatory cell, interleukin-8 (IL-8) and eotaxin in sputum were determined. The lung function was measured. Twenty healthy subjects served as controls. The counts of neutrophils and eosinophils, and the levels of IL-8 and eotaxin in sputum of AECOPD were higher than those of health subjects. There was a positive correlation between the levels of IL-8 and the counts of neutrophils (P < 0. 01), and between the levels of eotaxin and the counts of eosinophils in sputum ( P < 0.01). The levels of IL-8 and eotaxin were negatively correlated with the lung function (FEV1% ).

Two recipients of orthotopic heart transplantation have all recovered from their serious heart disease and returned to their previous work with good condition for 3 and 1 year respectively.This article discusses with emphasis some steps happened before,during and after the operation of heart transplantation,especially the donor heart protection,immunosuppressant administration,diagnosis and treatment of rejection.

The professional skill schools started an activity,which is the important measure to popularize the strategy of“the science and education making our country prosper”,that is,the definition of professional skill and implement of institution of professional certificates.It is the development direction of professional education that implementing of the institution of double-certificates',creating the new mode of cultivating the individuals,and strengthening the tuition of students'skill to make the students to acquire the education diplomas and the pro- fessional certificates.This issue points out the way and some opinions in this work,in the terns that imploring practice of the identification of the professional skill of microorganism inspecting worker' and the joint of microorganism education reform.

Diphtheria-Tetanus-Pertussis Vaccine ; adverse effects ; Female ; Hematuria ; etiology ; Humans ; Infant

Objective To investigate the effect of high-flux hemodialysis (HFHD) on advanced oxidation protein products (AOPPs) in maintenance hemodialysis (MHD) patients.Methods 40 MHD patients (MHD group),50 non-dialytic chronic kidney disease (CKD) patients (CKD group) and 31 healthy subjects undergoing health examination survey received AOPPs detection.After lowflux hemodialysis (LFHD) for 3 months,HFHD was performed in MHD group for 3 months.Plasma AOPPs and other biochemical indicators were tested at the end of 0,3 and 6 months.AOPPs levels were detected in HFHD and LFHD dialysates.Results Plasma AOPPs level was higher in MHD group than in CKD and control groups,and plasma AOPPs level was higher in CKD group than in control group.Compared with before LFHD in MHD group,AOPPs and triglyceride (TG) levels had no significant differences after LFHD for 3 months(t=0.831,-0.191,both P＞0.05),while plasma cholesterol (CHO) and β2 MG levels were increased in MHD group after LFHD for 3 months(t＝-2.697,10.39,P＜0.05 or 0.001).There were significant differences in plasma AOPPs,TG,β2-MG and CHO levels before versus after HFHD for 3 months (t=4.292,2.271,8.297 and 2.295,P ＜0.001 or 0.05).No AOPPs were detected in HFHD and LFHD dialysates.Conclusions Plasma AOPPs levels are increased in CKD patients.LFHD can not effectively eliminate AOPPs,while long term HFHD can decrease the plasma levels of AOPPs,TG and CHO,and reduce β2-MG accumulation.

[Objeetive] To investigate the effect of minimally invasive V-shaped osteotomy instead of traditional operative technique for correction of ankylosing kyphosis.[Method]The C-arm X-ray was used under the local anaesthesia to select the space of osteotomy,the length of incision were 6~8 cm and only exposed one interlaminal space,the width of laminar V-shaped osteotomy were 8~10 mm.The operating table was changed from reverse V to type after finished osteotomy,the space of osteotomy would be automatica ly closed and replaced,if the space could not be automaticly closed,careful manipulation would be applied to close it.The bone mass of osteotomy was used in posterior bone graft of lamina.Postoperation,stric and standardized regulation were performed to send the patient back to ward.The fractional manual correction and external fixation of hyperextended plaster vest were performed respectivly 2 weeks after operation.[Result]Fifty patients of this group acquired more satisfied results except 1 case appeared recurrent deformity due to early removing of the plaster vest.[Conclusion]Minimal damage,lower medical expenses(without internal fixation)and satisfied results demonstrated the minimally invasive V-shaped osteotomy is a very effective method.It has advantages of Mutual intercalated V-shaped osteotomy space and better stability after reduction,posterior bone graft of lamina and external fixation of hyperextended plaster vest,therefore,all patients can acquire the bone graft fusion afer 6 months.