Animals ; Bone Marrow Cells ; cytology ; Cell Differentiation ; physiology ; Humans ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stromal Cells ; cytology ; Myocardial Infarction ; surgery ; Myocytes, Cardiac ; cytology

Objectives To observe the correlation among the level of serum apoH and liplid parameters in patients with cerebral infarction and study the relationship between the serum apoH level and cerebral infarction.Method The serum levels of apoH in all subjects were measured by using radioimmunoassay.Results Mean apoH level of the patients in actue and convalescent cerebral infarction was increased remarkably as compared with both stroke dangerous factor control group and normal control group(P

[Objective]To analyze clinical features,evaluate diagnostic procedures and discuss the different forms of therapy for each case of split cord malformation(SCM).[Method]A series of 48 patients had a follow-up period of 0.5~9 years,29 males and 19 females,aged 7 days to 14 years,were evaluated retrospectively.They suffered from back midline abnormalities,neurological deficits of lower limbs and bladder dysfunction.At first they were cut filum terminale and then excised the fibrous or bony spur finally treated scoliosis.The radiologieal procedures used for the diagnosis of SCM were spinal X-ray,ultrasound,CT scan and MRI.[Result]In all of the 48 patients with tethered cord,there were 28 cases with dysplasia of lower limbs and feet,13 cases with urination and defecation dysfunction,14 cases with only dorsal-lumbar abnormalities,26 cases with scoliosis and kyphosis,14 with myelomeningocele,15 with syringomyelia and 12 with intraspinal lipomas.There were 27 cases suffering from type Ⅰ SCM,18 type Ⅱ SCM and 3 composite type SCM according to Pang.The most frequent location of the spur was at the lumbar level(38 cases),the less frequent in thoracic regions(6 cases)and exceptionally rare below S_1(2 cases),in the cervical regions(1 case),the thoralumbar(1 case).After operation,28 cases were improved,3 cases were cured and 3 cases were inefficacy.[Conclusion]The most frequent syndrome of SCM patients is lower limbs dysplasia and dorsal-lumbar abnormalities.The patients are most suffered from tethered cord and spinal deformities.To improve diagnosis of SCM,tethered cord and other deformities,the patients should be routinely evaluated by MRI and X-ray of the whole spine,sometimes could be evaluated by ultrasound and CT scan.SCM cases should be early treated by first cutting filium terminal and then excising fibrous or bony spur and hemivertebra.

The results of lysine, phenylalanine, tyrosine, and valine on kinetics of zinc absorption in rat ileurn using a two-compartment in situ perfusion model showed; 1. Phenylalanine can increase the zinc absorption micro-constants K21, K12, and K02, That is to say phenylalanine increases the a-mount of zinc from lumen to mucosa, from mucosa to lumen, and from mu-cosa to blood. Tyrosine and valine can increase the values of K21, so more zinc flows from lumen to mucosa; 2. The results of amino acids on the half times of zinc absorption showed that phenylalanine decreases both the half times of the fast phase and the slow phase, while valine decreases only the half time of the fast phase. Lysine and tyrosine have no effects on the half times of zinc absorption. The results of this study indicate that phenylalanine, tyrosine, and valine increase zinc absorption in the ileum of rat. The enhancing effects of amino acids on zinc absorption are related to the stability constants of zinc-amino acid chelators.

Abnormalities, Multiple ; Bronchi ; abnormalities ; Bronchography ; Constriction, Pathologic ; diagnostic imaging ; Diagnosis, Differential ; Humans ; Infant ; Male ; Rare Diseases ; Tomography, X-Ray Computed ; Trachea ; abnormalities ; diagnostic imaging ; Tracheal Stenosis ; congenital ; diagnostic imaging

Objective:To evaluate the diagnostic efficacy of SPIO in known liver lesions.Methods:30 patients with known liver lesion were underwent MRI.The contrast noise rate(CNR)of lesion to liver and the relative enhancement rate(RE)of liver were measured at 10、30、45、70 min and 16 hour after SPIO was injected.The dynamic curve of signal-noise were made and analysed.Results:The study shown that the hepatic parenchyma had a significantly negative enhancement in T 2 weight of SE and GRE sequence.However,the signal intensity showed few changes in hepatic metastases and only a little positive or negative enhancement in angioma、HCC and FNH.Conclusion:SPIO,as a negative contrast media for liver,has a significantly efficacy to show liver lesions,and as liver lesion-specific contrast MR agent,is worth being explored. [

Objective:To identify the function of extracellular soluble vimentin that promotes proliferation, activation and chemotaxis of inflammatory cells. Methods: The proliferation of rat splenocytes stimulated with vimentin was evaluated in vitro. The lymphocyte counts and vimentin-antibody levels of peripheral blood in mice immunized with vimentin were detected in vivo. Peritoneal macrophages were collected and cultured with different concentrations of vimentin to detect the effect on phagocytosing chicken red blood cells. The chemotaxis of NIH3T3 fibroblast towards vimentin was observed in transwell chamber. Results:In vitro vimentin dose dependently promoted the proliferation of splenocytes. The proliferation indexes of primed and naive splenocytes cultured with 16μg/ml vimentin were reached up to ( 196. 0 ± 9. 7 )% and ( 208. 9 ± 4. 6 )% respectively without significant difference. In vivo vimentin significantly enhanced the lymphocytes number(109 L-1)of peripheral blood(5. 74±0. 51 vs. 1. 69±0. 13)and the levels of vimentin specific antibody( OD value 2. 31 ± 0. 06 vs. 0. 19 ± 0. 08 ) that shown no significant difference from immunization with vimentin plus CFA. In vitro vimentin dose dependently stimulated phagocytic ability of macrophages and performed the chemotactic effects on NIH3T3 fibroblasts. Conclusion:Extracellular soluble vimentin promotes the proliferation,activation and chemotaxis of concerned inflammatory cells and possesses the functions as an alarmin.

Objective To evaluate the characters of respiratory function in children with chronic cough and explore the correlative association between cough variant asthma(CVA) and etiology of chronic cough.Methods One hundred and forty patients with chronic cough were divided into 2 groups based on peak expiratory flow(PEF) or forced expiratory volume in one second(FEV1).Ninty-three cases were done exercise test and 47 cases were done bronchoditor rest.The parameters included forced vital capacity(FVC),FEV1,PEF,forced expiratory flow at 50% and 75%(FEF50,FEF75).Results The measurement 35 cases with positive bronchoditor rest,and 30 cases with positive exercise test were found.The PEF and FEV1 variation rate were(18.30?10.50)%,(18.78?9.44)% in exercise test groups,and(30.36?27.27)%,(36.13?26.83)% in bronchoditor rest groups,respectively.Conclusions FEV1,PEF may be used as markers for the reversibility of airway obstructe in children with CVA.There is significant correlation between PEF and FEV1.Respiratory function mensurate may reflect the change and degree of inflammation in the airway of children with chronic cough.

Objective To investigate the infiltration of inflammatory cells and the pathogenesis of nasal polyps. Methods the nasal polyps were obtained from 42 patients undergoing nasal operations. The tissues from each patient were stained with HE and Toluidine Blue for eosiophils, lymphocyte, plasma cell and mast cell and observed under the light microscope. A mean number of the above cells were counted. All data were analyzed with the Ttest. Results Eosinophils were found predominantly in 12 polyps(28.57％). Lymphocyte infiltration was shown in the other 30 polyps (71.43％). Furthermore, many mast cells with only a few eosinophils were found in the lymphocyte infiltration polyps; the number of mast cells in these polyps were much more than those in eosinophil accumulation polyps with a very significant difference(P＜0.01). Conclusion The accumulation of eosinophils in the nasal polyps does not appear to be related to the presence of mast cells and allergy. The disorder of immunity may play an important role in the formation of nasal polyps.

BackgroundHypoxia-inducible factor-1 α (HIF-1α) specific double-stranded RNA ( dsRNA ) mediated by liposome inhibit reinal neovascularization in mice at dose-dependent manner. ObjectiveThe present study was to investigate the inhibitory effect of dsRNA targeting HIF-1α on retinal neovascularization in mice.MethodsModels of oxygen-induced retinal neovascularization were set up in C57BL/6J mouse through exposure of postnatal day 7 ( P7 ) to (75±3) ％ oxygen for 5 days.Fluorescein conjugated Dextran angiography of retinal vascular was performed to identify the retinal neovascularization.The 8 mice of the normal group were raised in the room air.Fifty-one P7 mice exposed to(75±3)％ oxygen for 5 days and then returned to the room air and assigned to control group ( 3 mice),empty vector group( 3 mice) and gene therapy group (45 mice),and the latter were medially divided to 9 groups randomly according to dose-ratio ( liposomes ∶ plasmid).The pSilencer 2.1-U6 hygro was injected in the model mice of empty vector group,and different dose-ratios of pSilencer2.1-U6 hygro-HIF-1α dsRNA were injected respectively in the model mice of various gene therapy groups.Fluorescein conjugated Dextran angiography of retinal vascular was performed to observe the morphology of new blood vessels,and retinal slides were prepared to score the numbers of nuclei extending beyond the inner limiting membrane( ILM ),and expression of vascular endothelial growth factor(VEGF) was detected in the retina by immunohistochemistry.Results The retinal blood vessels of the normal group formed a fined radial branching pattern.The retinal vascular patterns in the control group and the empty vector group were characterized by decreased central perfusion in both the superficial and the deep layers.The abundant vessels were distorted and irregular in the control group and empty vector group,and the obstructed capillary and lots of neovascular tufts were seen.The retinal neovascularization and non-perfusion distraction in the every gene therapy group were reduced markedly with the most severe appearance in 1 ∶ 1 ( liposomes ∶ plasmid) dose-ratio group.Few vascular endothelial cell nucleus extending beyond the ILM were found in the normal group;while a large number of vascular endothelial cell nucleus were showed in the control group and empty vector group with the occurring rate 100％.Statistically,no significant difference was seen in the number of nuclei extending beyond the ILM between the control group and the empty vector group(11.57±5.85 vs 11.53±6.15),however,that in 1∶1 (liposomes∶plasmid) group was reduced markedly ( 2.17 ± 4.23 ) ( P ＜ 0.01 ).Immunohistochemistry revealed that VEGF was faintly expressed in the normal group but strongly expressed in the control group and the gene therapy group.VEGF expressions of various gene therapy groups were weaker than ones of the control group and the empty vector group.ConclusionsRetinal neovascularization can be efficiently inhibited by intravitreal injection of the pSilencer2.1-U6 hygro-HIF-1α dsRNA mediated by liposome.Proportion of 1 ∶ 1 (liposomes ∶ plasmid)has a maximized efficiency.