ObjectiveTo study the missing items in questionnaire cfidentification for TCM constitution.MethodsThe questionnaires were regarded as study objects,which were independently finished by 1322 participants in Changfeng community who were over 45 years and agreed to participate in.The data'was analyzed by SPSS.ResultsMissing items were found in the questionnaires,with a missing rate of 33.74％ on an overall basis.No statistic difference was noted between male and female (x2=0.67,P＞0.05).The rate of missing items increased as participants' age climbing up to the age of 80,and then decreased.Groups characterized with age and gender showed difference in missing items.89.69％ of the incomplete questionnaires had less than 3 missing items,which concentrated in some specific items.ConclusionAttention should be addressed to the fact that missing items were found in questionnaircs of identification for TCM constitution finished independently by residents.

AIM: To examine the changes in immune cells which might be involved in systemic immune dysfunction after severe burns, we detected the sequence of expressed sequence tag in lymphocytes and monocytes of F344 rats post-burn. METHODS: Two pairs of primers were designed depending on the sequence of AI764697.1. SMART RACE technology was employed for amplification of whole sequence. Furthermore, the amplified sequence was testified by Northern blot. RESULTS: A 592bp sequence, which was testified by Northern blot, was suceessfally obtained in our experiments. This product was accepted by GenBank and its accession number is AF244895. CONCLUSION: A cDNA whole sequence in immune cells of F344 rats after severe burns was amplified successfully. More research work should be done to identify the resource, location in genome and function of this product.

Objective To study the optimum process for Suanzaoren granules,and to optimize the ethanol-extracting process for jujuboside A in Semen Ziziphi Spinosae.Methods With the extracting rate of jujuboside A as the index,HPLC was used to determine the content of jujuboside A in extracted solution and the extracting conditions were optimized by orthogonal design.Results The optimum extracting condition was adding 8 times amount of 70% ethanol and refluxing for 60 min,then adding 6 times amount of 70% ethanol,refluxing and extracting for 40 min(two times).Conclusion The optimized process is stable and highly efficient.

Objective To explore drinking water pollution by algae in Wuhan. Methods Sampling was carried out in different kinds of tap waters and tank waters for algae detection and identification. Results The detection rate of algae in tap waters was 89% in tank water it was 86% and at the same spot the genera and number of algae in the tank waters increased by 37% and 60% compared with the tap waters respectively. Conclusion Drinking water has been polluted by algae in different degree in Wuhan.

Objective: To investigate the expression of angiopoietin-related growth factor (AGF) in obese patients and its relationship with body mass index(BMI), waist circumference(WC), waist-hip ratio(WHR), blood glucose(FBG), plasma insulin(FINS) levels, and HOMA-insulin resistance index(HOMA-IR). Methods: Plasma AGF levels were assayed by ELISA in 40 patients with obesity and 40 normal controls. The relationship of AGF level with BMI, WC, WHR, HbA 1c, blood lipids, FBG, FINS, and HOMA-IR was also analyzed. Results: AGF levels in patients with obesity were significantly increased compared with that in the control group ([177.55±74.09] ng/ml vs [122.37±66.91] ng/ml, P<0.05). We also found that AGF level was positively correlated with Triglyceride(TG), FINS and HOMA-IR(r=0.325, P<0.05; r = 0.451, P<0.01; r = 0.483, P<0.01); and was negatively correlated with high-density lipoprotein cholesterol(HDL, r= -0.529, P<0.01). Multiple regression analysis showed that HDL, BMI and FINS were the factors influencing AGF levels. Binomial Logistic regression analysis indicated that AGF level was positively correlated with obesity after adjusting sex, age, total cholesterol (TC), TG, HDL and low-density lipoprotein cholesterol (LDL) (OR>1). Conclusion: The change of AGF level is associated with the metabolism disorder in obese patients, and it may also contribute to the pathogenesis of obesity.

Objective To explore the value of video-assisted thoracoscopy for the diagnosis of indeterminate solitary pulmonary nodules.Methods A total of 42 patients with indeterminate solitary pulmonary nodules(

Objective To investigate the expression and role of Caspase-3 during the development of non-traumatic avascular/aseptic necrosis of femoral head(ANFH).Methods Tissue samples were obtained from 22 femoral heads of 21 patients with ANFH.The samples were divided into three groups:early stage(6 cases),middle stage(8 cases) and late stage(8 cases) groups according to the progress of the disease.Meanwhile,19 tissue samples of 19 femoral heads were obtained from 19 cases of acute femoral neck fracture and set as a control group.The level of apoptosis of each group was compared in terms of the percentages of TUNEL positive osteocytes and empty osteocyte lacuna,and then the activity of Caspase-3 was detected by colorimetric method.Results Both the pecentages of empty osteocyte lacunae(F=45.43,P=0.000) and the TUNEL positive osteocytes(F=120.86,P=0.000) increased significantly with the progress of ANFH.The two indexes in the early and middle stage groups were significantly higher than those in the control(q=18.899,P0.05).Caspase-3 activity was not significantly related to the capacity of osteogenesis(r=0.126,P=0.425).Conclusions Caspase-3 is not the major executor of apoptosis in ANFH.It is very likely that it does not play a nonapoptotic role in the progress of the diseases.

Objective To investigate the influence of renal function on serum NT-proBNP in the diagnosis of chronic heart failure by observing the relationship between eGFR and NT-proBNP in serum and comparing cutoff values of NT-proBNP in different eGFR levels.Methods 297 elderly participants were enrolled in the study, including 106 inpatients with heart failure and 191 healthy controls in the corresponding period.The concentration of NT-proBNP was determined by an automated electrochemiluminescence immunoassay on Roche Elecsys 2010.Estimated glomerular filtration rate (eGFR) was calculated using the Levey-modified Modification of Diet in Renal Disease formula. The diagnosis of clinical physician was considered to be the golden standard for heart failure.Results Serum NT-proBNP levels were significantly higher in subjects with renal dysfunction compared with those with normal renal function for heart failure patients [829.1 (202.4 ~ 3725.5) ng/L vs. 227.2 (111.1 ~ 964.2) ng/L, P

AIM:The limitation of materials' strength results in the external fixation of patients with patellar fracture following absorbable screw fixation.This study was aimed to guide the patients do progressive rehabilitation and observe the recovery of knee joint function.METHODS:Totally 35 patients with patellar fractures were enrolled from the Sixth People's Hospital in Shanghai Jiao Tong University between June 2004 and June 2006,including 27 cases of transverse fractures and 8 cases of comminuted fractures.They were all treated with BIOFIX self-reinforced poly-lactide absorbable screws,which were produced by Finland BIONX Company.Postoperatively isometric contraction training of quadriceps femoris was followed,and then progressive knee joint range of motion was carried out.The curative effect was evaluated by the recovery of knee joint function and radiograph.Normal knee joint function was taken as excellent effect,while nearly normal knee joint function and slightly poor squatting were taken as good effect.RESULTS:All the patients completed the treatment and rehabilitation,followed up for no less than 12 months,with a mean of 22 months.The average healing time was 6 weeks,excellent result cases were seen in 29 patients and good in 6.There was two cases occurred postoperative complications(wound swelling).CONCLUSION:Self-reinforced poly-lactide absorbable screws for treatment of patellar fractures and appropriate postoperative rehabilitation is believed to prevent the second operations.

Objective To explore the related factors about acute stress responses of the military freshmen,then built a path model among self-efficacy,social support,coping style,state-trait anxiety and acute stress responses.Methods 584 students were tested by General Self-efficacy Scale (GSES),Perceived Social Support Scale (PSSS),Coping Styles Questionnaire,State-Trait Anxiety Inventory (STAI) and Acute Stress Response Scale ( ASP S).Results ①The military freshmen in each factor of acute stress response got a lower score,especially the mean score of the changes of cognition was higher (0.21 ±0.21 ),compared with the other factors,and the changes of pathology (0.053 ±0.114) was lower.②There were significant correlations between self-efficacy,social support,positive coping,negative coping,state anxiety,trait anxiety and acute stress responses (P ＜ 0.01 ).③ Negative coping( r=0.130),trait anxiety(r=0.005) and state anxiety(r=0.002) influenced ASR directly,meanwhile,self-efficacy( r =-1.292) influenced ASR through the state anxiety indirectly,self-efficacy ( r=-7.465 ) and social support ( r =-0.294) influenced ASR indirectly through trait anxiety.Conclusion Acute stress responses of the military freshmen were directly influenced by negative coping and state-trait anxiety,and negative coping style was the main reason.