Objective To study the optimum process for Suanzaoren granules,and to optimize the ethanol-extracting process for jujuboside A in Semen Ziziphi Spinosae.Methods With the extracting rate of jujuboside A as the index,HPLC was used to determine the content of jujuboside A in extracted solution and the extracting conditions were optimized by orthogonal design.Results The optimum extracting condition was adding 8 times amount of 70% ethanol and refluxing for 60 min,then adding 6 times amount of 70% ethanol,refluxing and extracting for 40 min(two times).Conclusion The optimized process is stable and highly efficient.

AIM: To examine the changes in immune cells which might be involved in systemic immune dysfunction after severe burns, we detected the sequence of expressed sequence tag in lymphocytes and monocytes of F344 rats post-burn. METHODS: Two pairs of primers were designed depending on the sequence of AI764697.1. SMART RACE technology was employed for amplification of whole sequence. Furthermore, the amplified sequence was testified by Northern blot. RESULTS: A 592bp sequence, which was testified by Northern blot, was suceessfally obtained in our experiments. This product was accepted by GenBank and its accession number is AF244895. CONCLUSION: A cDNA whole sequence in immune cells of F344 rats after severe burns was amplified successfully. More research work should be done to identify the resource, location in genome and function of this product.

ObjectiveTo study the missing items in questionnaire cfidentification for TCM constitution.MethodsThe questionnaires were regarded as study objects,which were independently finished by 1322 participants in Changfeng community who were over 45 years and agreed to participate in.The data'was analyzed by SPSS.ResultsMissing items were found in the questionnaires,with a missing rate of 33.74％ on an overall basis.No statistic difference was noted between male and female (x2=0.67,P＞0.05).The rate of missing items increased as participants' age climbing up to the age of 80,and then decreased.Groups characterized with age and gender showed difference in missing items.89.69％ of the incomplete questionnaires had less than 3 missing items,which concentrated in some specific items.ConclusionAttention should be addressed to the fact that missing items were found in questionnaircs of identification for TCM constitution finished independently by residents.

Objective To explore drinking water pollution by algae in Wuhan. Methods Sampling was carried out in different kinds of tap waters and tank waters for algae detection and identification. Results The detection rate of algae in tap waters was 89% in tank water it was 86% and at the same spot the genera and number of algae in the tank waters increased by 37% and 60% compared with the tap waters respectively. Conclusion Drinking water has been polluted by algae in different degree in Wuhan.

Objective: To investigate the expression of angiopoietin-related growth factor (AGF) in obese patients and its relationship with body mass index(BMI), waist circumference(WC), waist-hip ratio(WHR), blood glucose(FBG), plasma insulin(FINS) levels, and HOMA-insulin resistance index(HOMA-IR). Methods: Plasma AGF levels were assayed by ELISA in 40 patients with obesity and 40 normal controls. The relationship of AGF level with BMI, WC, WHR, HbA 1c, blood lipids, FBG, FINS, and HOMA-IR was also analyzed. Results: AGF levels in patients with obesity were significantly increased compared with that in the control group ([177.55±74.09] ng/ml vs [122.37±66.91] ng/ml, P<0.05). We also found that AGF level was positively correlated with Triglyceride(TG), FINS and HOMA-IR(r=0.325, P<0.05; r = 0.451, P<0.01; r = 0.483, P<0.01); and was negatively correlated with high-density lipoprotein cholesterol(HDL, r= -0.529, P<0.01). Multiple regression analysis showed that HDL, BMI and FINS were the factors influencing AGF levels. Binomial Logistic regression analysis indicated that AGF level was positively correlated with obesity after adjusting sex, age, total cholesterol (TC), TG, HDL and low-density lipoprotein cholesterol (LDL) (OR>1). Conclusion: The change of AGF level is associated with the metabolism disorder in obese patients, and it may also contribute to the pathogenesis of obesity.

Chronic atrophic gastritis (CAG), a chronic disease of the digestive system resulting from multi-pathogenic factors, is precancerous state of gastric cancer. Authors reviewed the current situation of Chinese medical diagnosis and treatment of CAG, and looked forward to its prospect by combining with their own clinical experience and scientific researches.
Chronic Disease ; Gastritis, Atrophic ; diagnosis ; therapy ; Humans ; Medicine, Chinese Traditional ; Precancerous Conditions ; Stomach Neoplasms

Objective To probe into the indication, operative skill of intrapericardial pneumonectomy, the prevention and cure in postoperative complication of intrapericardial pneumonectomy for locally advanced lung canc-er. Methods The clinical data of 43 cases of locally advanced lung cancer undergoing intrapericardial pneumonec-tomy from February 1998 to November 2007 were retrospectively analyzed. Results No surgical death occurred. The main postoperative complication was arrhythmia, acute pulmonary edema and pneumonia, with incidence as 41. 86%. The 1-,3- and 5-year postoperative survival rate was 79.1% ,30.2% and 11.6%. Conclusions The intrap-ericardial pneumonectomy skill not only can improve resection rate, reduce exploration rate, but also offer more oppor-tunity of further therapy in a part of locally advanced lung cancer.

Objective To investigate the expression and significance of survivin and CD44v6 in non-small cell lung cancer (NSCLC) and their correlation. Methods SP immunohistochemical technique was used to detect the expression of survivin and CD44V6 protein in 53 cases of NSCLC and 13 cases of para-eaneer nor-mal tissues. Results The positive rate of survivin and CD44v6 in NSCLC was 60. 38% and 69. 81% respec-tively,which was higher than that of normal pulmonary tissues adjacent to carcinoma(P <0. 01). The expres-sion of survivin and CD44v6 in NSCLC was related to lymph node metastasis (P < 0. 05), but not to patients' age and gender(P>0.05). The expression of survivin was related to TNM stages and cell differentiation (P <0. 05) ,but not to histology type(P >0. 05). The expression of CD44v6 in squamous carcinoma was significantly higher than that of adenocareinoma (P <0. 05),but the expression of CD44v6 was not related to TNM stages and cell differentiation (P >0. 05). There was no correlation between the expression of survivin and C1)44v6 (r = -0. 058, P >0. 05). Conclusion Survivin might be used to evaluate NSCLC development;CD44v6 might be used for the differential diagnosis of squamous carcinoma in NSCLC;both of them might be helpful to predict the metastasis of NSCLC. They might be two independent events in the process of NSCLC genesis and develop-ment.

BACKGROUND: Although cultured technique and purified method of Schwann cells (SCs) are changed successively,purity is still limited. Whether double negative selections can culture high-purified SCs still needs a further study.OBJECTIVE: To investigate the effects of double negative selections on growth and purity of SCs.DESIGN: Controlled animal study.SETTING: Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University,and the Beijing Institution of Otolaryngology.MATERIALS: The experiment was carried out in the Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University and the Beijing Institution of Otolaryngology between May 2006 and July 2006. Twenty new-born Wistar rats of 3 days (without gender limitation) were provided by the Experimental Animal Center of the Chinese Medical Academy.METHODS: The sciatic nerves of rats were digested by 0.2% collagenase. ① Grouping: All the rats were divided into 4 groups with 5 each. The experimental group was depurated by differential adhesion and Ara-c for 30 minutes. SCs in the control group 1 and 2 were managed by differential adhesion and Ara-c for 30 minutes, respectively. Cells in the control group 3 were not purified. ② Evaluation: The cell growth was observed under invert microscope; growth curves were measured with MTT method in the experimental group and control group 3 to observe cell proliferation. In addition, the SCs were identified by immunocytochemistry; the culture purity was calculated (ratio of S100 positive cells in each sight).MAIN OUTCOME MEASURES: ① Growth of SCs; ② immunocytochemistry stain of SCs; ③ growth curve of SCs; ④ purity of SCs.RESULTS: ① Growth of SCs: At 5 days after incubation, the SCs proliferated well. SCs were spindle, bipolar and sometimes tripolar, with obvious nuclei and a little cytoplasm. There was more fibroblast cell in the group 3 than that in experimental group. ② Growth curve: The SCs in experimental group entered into the logarithm multiplication period at the third day. ③ Immunocytochemistry: The positive-stained SCs were brown in its body and process, and arranged like swirl. The FB with spread and anomaly shape was slight blue. ④ The ratio of anti-S100 positive cells was higher in experimental group than that in group 3 (P ＜ 0.01).CONCLUSION: With the double negative selection, high-purified SCs are obtained effectively.

Purpose　To detect the prevention of EGF and GLN on the side effect of TPN.　Methods　We randomized SD rats into four groups:group A (control),B(STPN),C(TPN+GLN),D(TPN+GLN+EGF).All rats were maintained on their respective diets for 8 days,at which time they were killed and the proximal jejunum was incised for biopsy,the MLN and celiac vein blood were collected for bacterial culture(aerobic culture).　Results　Group B rat mucosal cell atrophied,DNA and RNA content and plasma cell decreased,the positive rat of MLN bacterial culture was 62.5%.Atrophy in group C alleviated and bacterial translocation rate declined(37.5%).Group D was similar with group A.　Conclusions　GLN combined with EGF is more effective in protecting gut barrier function and preventing bacterial translocation than GLN alone.