Objective To establish a reversed HPLC method to determine the contents of gatifloxacin and tinidazole simultaneously. Methods ODS C_ 18 column (4.6mm?250mm,5?m) was used as an analytical column. The mobile phase consisted of 15% acetonitrile, 25% of methanol and 60% of 0.05mol/L KH_2PO_4 buffer,which contained 0.5% triethylamine and was adjusted to pH 3.5 by phosphoric acid. The flow rate was 1ml/min. The detection wavelength was 318nm. The column temperature was 30℃. 20?l of diluted sample solution was injected to the sampler. Results The calibration curve of gatifloxacin was linear in the range of 2~20?g /ml (r=0.999 6) ,and that of tinidazole was 2~20 ?g /ml (r=0.999 9). The recovery rates of them were 100.1% and 100.4%,respectively. RSDs of both drugs were less than 1.1 %. Conclusions The method is simple,accurate and sensitive and can be used to determine the two components simultaneously.

Bone marrow-derived stem cells, mainly including hematopoietic stem cells, mesenchymal stem cells, multipotent adult progenitor cells, etc., are the most densely studied adult stem cells up to now. Recent findings indicate that adult stem cells have the potential to differentiate across lineage barriers and to adopt the expression profiles and functional phenotypes of cells unique to other tissues. This flexibility is termed "plasticity",which has posed challenges to conventional concepts. Meanwhile, it brings new hopes for the therapeutic application of cell engineering and tissue engineering. This review focuses on the biological features, the plasticity and its possible mechanisms, research method and potential clinical application of bone marrow-derived adult stem cells

Objective In order to assist the medical staff to take appropriate contents and ways of health education,and to promote the recovery of physical and mental health of children with attention deficit hyperactivity disorder (ADHD),the needs of the parents about how to help them acquire the related knowledge were investigated.Methods A survey on the degree of familiarity of nursing knowledge,the requirements and the ways for getting the knowledge about the health education was carried out.Results The rate of 226 parents who knew about nursing knowledge was 10.62％-87.61％ (24/226-198/226).More than half of the parents needed health education.The rates of concerns were as follows,92.48％ (209/226) of all the parents concerned on the factors affecting prognosis,94.69％(214/226) on the comorbidities,92.04％ (208/226) on the training methods of attention,and 91.15％ (206/226) on psychological and behavioral interventions.Conclusions Parents could acquire related knowledge and educational methods about ADHD from the medical staff and afford a good care,which may improve behavior problems and mental health of the children with ADHD.

Objective To explore the prevalence of qnr gene in gram-negative bacteria isolated from Renmin Hospital of Wuhan University. Methods qnr gene was detected by PCR in non-duplicate E.coli (n=129), E.cloacae (n=10) and K.pneumoniae (n=29) isolates.PCR technique was used to amplify the gene encoding type I integrons.Kirby-Bauer disk diffusion method was used to test the in vitro suscepti- bility of the isolates to 16 antimicrobial agents.ESBLs were investigated according to phenotypie screening and confirmatory test recom- mended by NCCLS.MIC values of ciprofloxacin were determined for qnr gene positive strains.Results qnr gene were identified in 6 clinical isolates including 5 strains of E.coli and I E.cloacae, qnr gene was not found in K.pneumoniae isolates.The 6 qnr-positive clinical iso- lates were positive for type I integrons.All the 6 strains were susceptible to imipenem but resistant to many other drugs tested.Only 2 of the E.coli isolates were susceptible to ciprofloxacin.Conclusions In Hubei Province, the prevalence of qnr is confirmed.The serious situa- tion of multidrug-resistance in clinical isolates with qnr gene requires careful monitoring of qnr-carrying isolates.

Blood Flow Velocity ; Child ; Coronary Aneurysm ; diagnostic imaging ; Humans ; Mucocutaneous Lymph Node Syndrome ; diagnostic imaging ; Ultrasonography, Doppler

China ; epidemiology ; Disease Notification ; standards ; Epidemiologic Methods ; Humans ; Models, Statistical ; Occupational Diseases ; epidemiology ; prevention & control ; Occupational Health Services ; statistics & numerical data ; Registries ; Wounds and Injuries ; epidemiology ; etiology ; prevention & control

ObjectiveTo investigate whether miR-25 targets KLF4 to regulate the growth of gastric cancer cells and lucidate the mechanism of miR-25 producing functional effects in gastric cancer cells. Methods The expression levels of miR-25 and KLF4 were detected by qRT-PCR in 35 pairs of gastric cancer tissues of patients. Target genes of miR-25 were predicted using Targetscan and verified though dual luciferase activity assay and west-ern blot. MTT assay and clone formation assay were detected after downregulated miR-25 and overexpressed KLF4 respectively. Results miR-25 was highly expressed in gastric cancer tissues with KLF4 downexpresstion. Downreg-ulated miR-25 and overexpressed KLF4 respectively can significantly reduce the proliferation of gastric cancer cells,the results were opposite when overexpression of KLF4. Conclusion miR-25 promotes gastric cancer cells proliferation by targeting KLF4.

BACKGROUND:Compared with single or composite biomolecular materials, decellulazied matrices are more biomimetic to natural ectocytic surroundings. So cel-secreted extracellular matrix is paid more and more attention in the field of tissue engineering, and the composition of these matrices are influenced by decellulezired preparation methods more or less. But there are few studies about the biological effect of different decellulazried methods on the extracellular matrix. OBJECTIVE:To investigate the composition of cel-secreted extracellular matrices in vitro by different decellularizing methods and their effects as surface modification on cytobiological reaction. METHODS:After the treatment of different decellularizing methods (freeze/thaw cycles, trypsin, weak alkal, detergents), the extracellular matrix was obtained and grouped into four kinds. The biological composition of the extracellular matrix was determined by ELISA assay. Then osteoblasts were seeded onto the four kinds of extracellular matrix surfaces. cells cultured normal y served as controls. The effect of extracellular matrix coatings on cellgrowth and differentiation were determined by MTT test and alkaline phosphatase activity test. RESULTS AND CONCLUSION:The residual components were the most in the freeze-thaw group, fol owed by the detergent and weak alkal groups, and the least in the trypsin group. Compared with the control group, the absorbance value of cells were lower in the freeze-thaw and detergent groups at days 3, 5, 7 after inoculation (both P<0.05);the alkaline phosphatase activity was lower in the trypsin group at days 5, 7 after inoculation (P<0.05), in the weak alkal group at 7 days after inoculation (P<0.05), and in the detergent group at days 3, 5, 7 after inoculation (P<0.05). Therefore, we can harvest more extracellular matrices by the freeze-thaw method, and the extracellular matrix coating synthesized by the freeze-thaw method is more helpful for cellgrowth than others.

In order to study the mechanisms of high frequency stimulation ( HFS), we designed an in vitro device to deliver extracellular electrical stimulation to live cells in culture, using the same parameters as in the surgical treatment of Parkinson's disease (PD). The system was made of a 24 wells culture plate electrified with titanium wire bridges or two titanium wires placed parallel in a culture dish of 10 centimetres diameter. The extremities of the series of wells were connected to a constant current generator. The result showed that this system had no toxicity on GH3 and PC12 cell cultures. Preliminary experiences demonstrate that this system is suitable for the study with DNA and protein chip technologies.

Objective:To investigate the corrosion fatigue strength of laser-welded specimens fabricated from commercially pure titanium (TA2) and titanium alloy(TC4). Methods:TA2 and TC4 were mechanically processed into a certain size and joined by laser welding. A fatigue testing machine was used to test tensile load and fatigue strength after soaked in synthetic saliva. The fatigue fracture surface was examined by scanning electron microscopy (SEM). Results: The fatigue strengths of the laser-welded test specimens showed no significant differences compared to specimens in air. SEM examination showed that there were no defects such as corrosion pits. Conclusion: There are no significant differences between either laser-welded metal in the corrosion fatigue strength for dry specimens and specimens in synthetic saliva.