Objective To investigate the applicative value of micro-surgery in the treatment of cerebral aneurysms.Methods During January 2011 to January 2013 in our hospital,90 patients with cerebral aneurysm were randomly divided into the endovascular group and the microsurgical clipping group (n =45 each).The endovascular group were treated with endovascular interventional treatment,and the patients of the microsurgical clipping group were treated with microsurgical clipping treatment.The aneurysm occlusion rate and recurrence,time of hospital stay,complications and prognosis were compared between the two groups.Results The complete occlusion rate was higher [97.8％ (44/45) vs.88.9％ (40/45) and the recurrence rate was lower 2.2％ (1/45) vs.13.3％(6/45) in microsurgical clipping group than in endovascular intervention group x2 =4.34 and 3.54,P=0.031 and 0.042),all P＜0.o5.The average time of hospital stay was longer in the endovascular intervention group than in the microsurgical clipping group [(19.3 4.4) days vs.(16.34.9) days,t 1.93,P=0.035].The good rate was higher in microsurgical clipping group than in endovascular intervention group [91.1％ (41/45) vs.77.8％ (35/45),x2=6.325,P=0.012].Conclusions Microsurgical clipping surgery can significantly raise the complete occlusion of cerebral aneurysms,reduce recurrence in patients with cerebral aneurysm,decrease the hospitalization time,and improve prognosis,which is worthy of clinical application.

Epidermal stem cells (ESCs) play a critical role in homeostasis and wound repair of skin tissue. Since ESCs are rare (fewer than 10% in total basal cells population) and lack specific markers,it is difficult to isolate and identify them from keratinocytes. Currently ,isolation of ESCs was achieved mainly by fast adhesion of ESCs to extracelluar matrix or flow cytometry. Several specific markers have been found in recent years for the identification of ESCs.

Ojective To evaluate the effect of the collagen injection for skin defect correction. Methods The various kinds of skin defect were injected by medical cosmetic collagen. Results 103 cases of skin defect were treated by collagen injection. Short term therapentic effect inclnding "excellent" and "good" was 85.5%. no complication occurred. Conclusion It showed that the method of collagen injection for skin defect correction is safe, simply and effective.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Tennis Elbow ; therapy

Objective:To improve the forming process of Jingning particle according to the influencing factors in its effectiveness, such as low yield, high moisture absorption and difficult dissolubility during oral administration. Methods:A new technology that im-mediate granulation after the extract was well mixed with lactose and dextrin and dried. The appearance, dissolubility, hygroscopicity and pellet formation rate ( granularity) were compared between the new technology and the old one, and the difference in critical rela-tive humidity was also studied. Results:The appearance, dissolubility and pellet formation rate of the new technology were all better than those of the old one, and the moisture absorption rate was reduced with the critical relative humidity up to 70%(25℃), which enhanced the granule stability. Conclusion:The pellet formation rate is improved by the new technology, which effectively solves the problems such as high moisture absorption and poor dissolubility, and the granule quality is improved.

Cucumber mosaic virus was detected from infected Basella rubra L. with the indirect enzyme-linked immunosorbent assay. Total RNA was extracted from infected leaves and the cDNAs of coat protein gene of CMV-Ba were obtained by RT-PCR. The amplified cDNA fragments were then cloned into pMD 18-T vector and sequenced,the result showed that the CP gene was 657 nucleotides in length. This sequence was aligned with the obtained CP gene and some CMV strains or isolates of subgroup Ⅰ and subgroup Ⅱ in GenBank using DNA MAN software. The results showed that CMV-Ba shared 90.9%~93.8% and 76.1%~76.9% identity with the known CP genes of subgroup Ⅰ and Ⅱ respectively in nucleotide level,on the other hand,amino acids deduced from CMV-Ba CP gene shared 92.7%~97.7% and 72.4%~78.1% identity with the known CP protein of subgroup Ⅰ and Ⅱ,respectively. This suggested that CMV-Ba CP gene belongs to CMV subgroupⅠ.

The chemical constituents of Vaccinium bracteatum were studied by means of macroporous resin, ODS column chromatography and preparative HPLC. Eleven compounds were isolated from this plant. By using ESI-MS and NMR, the structures of the eleven compounds were determined as 10-O-trans-p-coumaroyl-6alpha-hydroxyl-dihydromonotropein (1), 10-O-cis-p-coumaroyl -6alpha-hydroxyl-dihydromonotropein (2), vaccinoside (3), 10-O-cis-p-coumaroyl monotropein (4), isolariciresinol-9-O-beta-D-xyloside (5), tectoridin (6), vicenin-3 (7), quercetin-3-O-alpha-L-rhamnoside (8), quercetin-3-O-alpha-L-arabinopyranoside (9), quercetin-3-O-beta-D-galactopyranoside (10), and quercetin-3-O-beta-D-glucuronide (11), respectively. Compounds 1 and 2 are new, and compounds 4, 6 and 7 are isolated from the genus Vaccinium for the first time.
Drugs, Chinese Herbal ; chemistry ; Molecular Structure ; Spectrometry, Mass, Electrospray Ionization ; Vaccinium ; chemistry

Aneuploidy ; Breast Neoplasms ; genetics ; metabolism ; pathology ; Carcinoma in Situ ; genetics ; metabolism ; pathology ; Carcinoma, Ductal, Breast ; genetics ; metabolism ; pathology ; Cell Movement ; DNA Methylation ; DNA, Neoplasm ; genetics ; Female ; Humans ; Membrane Proteins ; metabolism ; Neoplasm Invasiveness

Breast Neoplasms ; genetics ; pathology ; Chromosome Aberrations ; Comparative Genomic Hybridization ; methods ; DNA, Neoplasm ; genetics ; Female ; Gene Dosage ; Humans ; Prognosis

Objective To establish the quality standards of Zizhu ointment. Methods The TLC was applied to identify Radix arnebiae and borneol of Zizhu ointment, and the content of borneol was determined by gas chromatography. Results The TLC spots were clear, well-separated and easy to identify. The good linear range of borneol reference substance on calibration curve was 0.048 4-1.210 0 μg, and the recovery was 90%-110%, the relative standard deviation was less than 5%. Conclusion The method is simple and feasible, can be used as the quality control method of Zizhu ointment.