OBJECTIVE:To compare the contents of trace elements in different parts of Piper kadsura.METHODS: Trace elements in leaf, micro-stem and macro-stem of P. kadsura were determined with inductive coupled plasma emission spectrometer. The contents of trace elements in the different parts were analyzed and compared. RESULTS: The content of Mg in the leaf was the highest among all trace elements, followed by Fe,Mn,Zn,Ti,Sr,Ba,Pb,B,Cu,Ni,Cr,Cd.The content of V in the macro-stem was higher than in the other parts. The contents of trace elements in micro-stem were all lower than in the other parts except of Sn. CONCLUSION: The leaf and stem of P. kadsura maybe has some complementary pharmacological function.

Objective:To evaluate the effects of insulin-like growth factor 1(IGF-1) in the pathogenesis of oral submucous fibrosis(OSF).Methods:IGF-1 mRNA in 27 OSF tissues and 9 normal tissues were detected by the in situ hybridization technique.Results:There were expression of IGF-1 mRNA in keratinocytes of all the 8 cases of early stage OSF and 7 of the 19 cases of middle-late stage OSF.No expression of IGF-1 mRNA was detected in keratinocytes of nomal tissue.There were no significant difference between OSF tissues and normal tissues in the expression of IGF-1 mRNA in fibroblasts.IGF-1 mRNA expression in endothelial cells were found in 5 cases of middle-late stage.Conclusion:IGF-1 might be secreted by keratinocytes and may play important role in the pathogenesis of OSF.

ObjectiveTo study the missing items in questionnaire cfidentification for TCM constitution.MethodsThe questionnaires were regarded as study objects,which were independently finished by 1322 participants in Changfeng community who were over 45 years and agreed to participate in.The data'was analyzed by SPSS.ResultsMissing items were found in the questionnaires,with a missing rate of 33.74％ on an overall basis.No statistic difference was noted between male and female (x2=0.67,P＞0.05).The rate of missing items increased as participants' age climbing up to the age of 80,and then decreased.Groups characterized with age and gender showed difference in missing items.89.69％ of the incomplete questionnaires had less than 3 missing items,which concentrated in some specific items.ConclusionAttention should be addressed to the fact that missing items were found in questionnaircs of identification for TCM constitution finished independently by residents.

Objective To investigate the risk factors of renal damage in children with Henoch-Schonlein purpura(HSP).Methods Two hundred and ninety-two children with HSP admitted to the First Affiliated Hospital of Anhui Medical University from Jan.2007 to Mar.2008 were reviewed.According to the findings urinary examination,children were divided into non-renal damage group(183 cases)and renal damage group(109 cases).Clinical data(gender,age,predisposing factors,rash repeated frequency,system damage)and laboratory examination [blood WBC,PLT,IgA,fibrinogen(FIB),TT,ASO,C3,C4] of 2 groups were compared,and then multiple regression analysis were performed to evaluate the risk factors of renal damage in children with HSP.Results 1.Among them,37.33% cases had renal da-mage,and hematuria and proteinuria manifestations were common.2.Simple Logistic regression showed that age was at least 8 years old and the number of purpura relapses was at least 4 times,increased serum FIB levels had significant difference between the 2 groups(P=0.030,0.000 and 0.011,respectively).There was no significant difference between the 2 groups in other clinical data and laboratory examinations.3.Mutiple Logistic regression showed that when the number of purpura relapses was at least 4 times,increased serum FIB levels were the risk factors of the renal damage(P=0.000 and 0.029,respectively);while there was no significant difference for the children who were at least 8 years old between the 2 groups(P=0.175).Conclusion When the number of purpura relapses is at least 4 times,increased serum FIB levels are the risk factors of the renal damage in children with HSP.

Objective] The mechanism of astragalus polysaccharides treatment of asthma was discussed. [Methods]Through the domestic and foreign about traditional Chinese medicine monomer astragalus polysaccharide on IL-4, IL-10 and other cytokines and inflammatory infiltrates aspects such as the Th1/Th2 balance, smooth muscle actin and airway mucus in the airway remodeling and asthma related gene research literature summary. [Results] Improve airway inflammation astragalus polysaccharides from activation and inhibition EOS EOS infiltration reduction, regulating Th1/Th2 imbalance, so Th0 cells to Th1 cell differentiation, and inhibiting the release of inflammatory cytokines and other aspects, APS can reduce airway hyper-responsiveness mitigate aspects peribronchial infiltration of eosinophils and airway smooth muscle cells induced byα-SMA expression and other high alleviate airway remodeling. [Conclusion] The influence of astragalus polysaccharide on asthma gene research will lay the basis for study of the mechanism of treatment of asthma, monomer research provides new ideas for traditional Chinese medicine.

Objective: To study the effect of melatonin (Mel) , a potent antioxidant, on the oxidative stress and the activity of hypothalamo-pituitary-adrenal axis in diabetic rats. Methods: Male SD rats were assigned to the following 4 groups: normal control (treated with 2% ethyl alcohol lavage[ NC, n=6 ]), diabetes mellitus control (treated with 2% ethyl alcohol lavage [DM, n=7]), diabetes mellitus model group 1 (treated with Mel lavage [10 mg · kg-1 d-1, DM+Mell, n=61), diabetes mellitus model group 2 (treated with Mel lavage [0. 2 mg · kg-1 · d-1 , DM+Me2, n=61). Diabetes in DM group, DM+Mell group and DM+Mel2 group was induced by intraperitoneal injection of streptozotacin (65mg/kg). Blood glucose, body weight and adrenal gland weight/body weight(AW/BW) were determined in the 4 groups 12 weeks later; malondialdehyde (MDA) was examined with visible spectrophotometry; blood total cholesterol (TC) and triglyceride (TG) were examined with enzymology method; blood corticotrophin-releasing hormone (CRH), adreno-corticotrophic hormone(ACTH) and corticosterone(CORT) were examined with radioimmune method. Results: Blood glucose, MDA , TC, TG, CRH, ACTH, CORT and AW/BW in DM group were significantly higher than those in NC group (P<0.01); and the body weight of rats in DM group were significantly lower those of NC group(P<0.01). Compared with DM group, DM+Mel1 and DM+Mel2 group had a significantly lower blood glucose, MDA TC, TG and AW/BW (P<0.01 or P

[Objective] To investigate the effect of Suiqing Pill (SP), a Chinese prescription with the actions of activating blood and removing blood-stasis, on growth and metastasis of tumor. [Methods] Sixty C57BL/6 mice were randomized to three groups: group A (model control), group B (low-dose SP) and group C (high-dose SP). H22 hepatocarcinomatous metastasis models were established by subcutaneous injection of H22 hepatocarcinoma cells into the right lower limb. The inhibitory rate of tumor weight, incidence of metastatic carcinoma of lung, metastasis of lymph node, survival time and life-span prolongation rate were observed. [Results] The weight of tumor was reduced and the inhibitory rate of tumor weight was 36.83% in group B (P

Objective To explore the clinical value of serum procalcitonin(PCT)and C-reactive protein(CRP)combination detec-tion in early diagnosis of liver cirrhosis complicating spontaneous bacterial peritonitis(SBP).Methods The peripheral blood sam-ples were collected from 30 cases of liver cirrhosis complicating SBP,30 cases of simple ascites liver cirrhosis (non-SBP)and 45 healthy subjects as control group.The serum PCT level was detected by the dry immunofluorescence quantitation method and the serum CRP level was detected by the immunoturbidimetry.The sensitivity and specificity of PCT,CRP and PCT combined with CRP in diagnosing liver cirrhosis complicating SBP were compared and the relationship between PCT levels and prognosis was ana-lyzed.Results Compare with the healthy control group and the non-SBP group,the serum PCT and CRP levels in the SBP group were markedly increased(P <0.05).The sensitivity of PCT,CRP and PCT combined with CRP in diagnosing liver cirrhosis compli-cating SBP was 93.3%,90.0% and 96.6% respectively,and the specificity was 90.0%,75.0% and 95.0% respectively.Conclusion The combination detection of serum PCT and CRP can increase the sensitivity and specificity of the diagnosis and has more clini-cal value in early diagnosing liver cirrhosis complicating SBP.

Objectiive:The present study was designed to explore whether overexpression of human wild ?-synuclein in rat brain caused selective dopaminergic neuron loss in substantia nigra and aimed to find out a new method to make a rat model of Parkinson's disease(PD).Methods:The human wild ?-synuclein gene was induced into the rat brain by Adeno-Associated Virus(AAV) vector.The overexpression of ?-synuclein was detected by realtime PCR.The behavior of rats were recorded every 4 weeks after the viral particle injection.TH immunohistochemistry were performed at 4,8,12 and 16 weeks post-injection as well as the dopamine(DA),3,4-dihydroxypheny-lacetic acid(DOPAC) of striatum were determined by high performance liquid chromatography coupled with electrochemical detection.Results:Realtime PCR results revealed a significant overexpression of ?-synuclein in the injected hemisphere.By 8 weeks post injection,a significant loss of the dopaminergic neurons was observed.34% of the dopaminergic neurons were lost after 12 weeks,and about 60% cells loss after 16 weeks.The DA and DOPAC levels in the striatum decreased about 15% 12 weeks after injecting viral particle carried ?-synuclein gene and 30% decreased after 16 weeks.The AAV-?-synuclein-treated rats developed a type of motor impairment,i.e.,head position bias,compatible with this magnitude of nigrostriatal damage.Conclusion:All the results showed that overexpression of human wild ?-synuclein caused selective dopaminergic neuron loss and mimic a symptom of human PD in rats.This may be a new methed to make rat PD model which can offer new opportunities for the study of pathogenetic mechanismsand exploration of new therapeutic targets of particular relevance to human PD.