Animal Experimentation ; Animal Testing Alternatives ; methods ; Animal Welfare ; Animals ; Toxicity Tests ; methods

Objective To investigate the curative effect of combined traditional Chinese and Western medicine treatment of de-pression after stroke.Methods Patients with depression in 134 cases of cerebral stroke from July 2011 to August 2012 in Tongli-ang People′s hospital were treated in department of internal medicine,and according to the different treatment methods were divided into experimental group and control group,each group of 67 cases.The control group was given paroxetine hydrochloride orally,the experimental group was based on the use of miananning granule in the treatment,obered curative effect of two groups.Results In the experimental group,the control group total effective rate were 95.5%,77.6%.The experimental group total effective rate was significantly better than the control group and has a statistically significance(P<0.05).Conclusion The combination of traditional Chinese and Western medicine treatment of depression after stroke is exact,it is worthy of popularization and application.

Objective To elucidate the mechanism of prevention of postoperative tissues from adhesion by chitosan by stadying the effect of chitosan on indncing apoptosis of fibroblasts.Methods The cultured fibroblasts were treated for 48 hours with 0,0.01,0.1,1,10mg/ml chitosan,respectively.Then cell proliferation was detected by MTT assay and the cell apoptosis by flow cytometry.Results When the concentrations of chitosan were 0.01,0.1,1,and 10mg/ml,the optical density(A)values of the fibroblasts were 0.377?0.047,0.324?0.030,0.271?0.035 and 0.224?0.037,respectively,which were significantly lower than that of control group(0.561?0.044,P

A tissue-tracking system can trace myocardial tissue using pattern matching method regardless of Doppler velocity information.The article shows data concerning clinical usefulness of the 2-dimensional tissue tracking(2DTT)technique in pediatric patients.2DTT is able to measure 3 directional(radial,circumferential,and longitudinal)strains of the myocardium in a pediatric population including neonates.Therefore,2DTT has clinical value for determination of normal pediatric cardiac myocardial strain.

OBJECTIVE: To prepare clonazepam oral disintegrating tablets and to evaluate its quality. METHODS: Gelatian and aspartame were used as tastes masking. Microcrystalline celluose, low substituted hydroxypropylcellulose and polyvininylpolyrrolidone were used as disintegrants. Clonazepam orally disintegrating tablets were prepared by wet granules. The in vitro and in vivo disintegration time, the relationship between hardness and disintegration time and the relationship between the usage of magnesium stearate and disintegration time were investigated, while taste and dissolution rate were also evaluated. RESULTS: When the gelatian/aspartame/poloxamer ratio was 30:0.5:1, the tablets had good oral feel and the uppermost dissolution rate. The dissolution rate of clonazepam oral disintegrating tablets which was evaluated by the method of common tablets could exceed 95% after 20 minutes. When the microcrystalline celluose / low substituted hydroxypropylcellulose / polyvininylpolyrrolidone ratio was 9:1: 3, the in vitro and in vivo disintegration time Was within 30 s. When the usage of magnesium stearate was 0.5% and the hardness ratio was in the range of 3-4 kg, disintegration time was within 30 s. CONCLUSION: According reasonable prescription and simple product craft, we can product qualified clonazepam oral disintegrating tablets.

Objective To investigate the association of gene polymorphism of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) with ulcerative colitis (UC) in Chinese patients. Methods The A+49G transition polymorphism at position 49 (exon 1) and C-318T transition polymorphism at position -318 in promoter of the CTLA-4 gene were determined by polymerase chain reaction with sequence specific primers (PCR-SSP) method in 82 Chinese patients with UC and 204 healthy controls of Han nationality. Results No significant differences in the distribution of genotype and allele frequencies were observed between CTLA-4 C-318T and A+49G gene polymorphisms in UC patients and normal controls. How- ever , comparing with healthy subjects,the frequency of haplotype 2,3 (C -318 -G 49 /T -318 -A 49 ) in UC patients was significantly reduced (26% vs 41%, P

Objective To investigate the status of the anxiety and depression in the patients with paralysis after spinal cord injury, and the measures to cope with it. Methods Sixty cases of patients with paralysis after spinal cord injury were evaluated with Self-Rating Anxiety Scale (SAS) and SelfRating Depression Scale (SDS), the results were compared with those of the domestic norm of healthy individuals. Results The scores of patients with paralysis after spinal cord injury were higher than that of domestic norm of healthy individuals. 54 cases had anxious symptom (90.00%), and 37 had depression.The causes led to anxiety and depression mainly came from the suffering of the disease, the influence of the work as well as the study and the cost of the treatment. Conclusions The patients with paralysis after spinal cord injury had mental problems, they need to be interfered with by healthy education and mental nursing in order to promote rehabilitation.

Objective To study the lymphocytes subsets including Th1 and Th2 cells in PBMC of children with bronchiolitis in acute period.Methods The present study enrolled 34 patients with bronchiolitis,23 of them were respiratory syncytial virus(RSV)and 11 non-RSV,and 22 age-matched normal infants.Fresh peripheral blood samples were treated and run through the flow cytometry.The percentage of lymphocytes subsets were acquired using simultest IMK-lymphcyte software.Results The results showed the percentage of CD3+ and CD4+ cells in bronchiolitis group was higher than that in control group(P

Objective:To observe the effects of Periploca forrestil Schltr Compound extract in serum interleukin-4(IL-4),interferon-γ(IFN-γ),tumor necrosis factor-α (TNF-α) and transforming growth factor-β1 (TGF-β1) in joint synovial tissue of rats,and further to investigate it′s therapeutic effects and related mechanism.Methods: To built the complete adjuvant arthritis (AA) model in rats,and then to detect the contents of IL-4,IFN-γ,TNF-α in serum by ELISA,TGF-β1 in synovial tissues of rats knee was detected by immunohistochemistry.Results: The contents of IFN-γ,TNF-α were higher and IL-4 and TGF-β1 were lower in adjuvant arthritis rats (model group) than those in blank control (P<0.01).By comparing with the model group the contents of IFN-γ and TNF-α were significantly lower in Periploca forrestil Schltr Compound treated rat group,whereas the expressions of IL-4 and TGF-β1 were increased(P<0.05).Conclusion: The Periploca forrestil Schltr Compound can significantly inhibit the expression of IFN-γ and TNF-α,and enhance the expression of IL-4 and TGF-β1,adjusted the Th1/Th2 cell and cytokines imbalance,to alleviate arthritis and cartilage destruction,therefore the Periploca forrestil Schltr Compound can significantly inhibit the proliferation of T lymphocyte and infiltration in synovial tissues of experimental rheumatoid arthritis.

Objective To determine the prevalence of dental anxiety in children patients before tooth extraction.Methods　A modified Children's Fear Survey Schedule-Dental Subscale (CFSS-DS) and Venham's clinical ratings of anxiety and cooperative behavior were used in 150 children patients ranging in age from 5 to 12 years olds before tooth extraction. The former scale was answered by parents on behalf of their children, and the latter ratings was assessed by the author. Results　CFSS-DS scores and clinical ratings of behavior of the children were significantly higher in the children whose oral hygiene condition, dental experience, tooth extraction experience, spirit status on that day and whose mother's educational background were bad or low. A step regression analysis showed that oral hygiene condition had most significant interrelationship with both of the two scales（P＜0.01）. The correlation of the two scales was high (r=0.67).Conclusion　Children’s oral hygiene condition, dental experience, tooth extraction experience, spirit status on that day and their mother’s educational background are closely related to the dental anxiety level. Oral hygiene condition is the most important predictor of anxiety level before extraction and clinical ratings of behavior during extraction.