Cyclooxygenase-2 (COX-2) plays an important role in the development of leukemia.COX-2 inhibitor plays a certain anti-leukemia role in the process.Many studies show that COX-2 inhibitor plays antileukemia role by inhibiting the proliferation of leukemia cell,promoting apoptosis,affecting cell cycle,inhibiting cell metastasis and reversing cell drug resistance and so on.Therefore,the researches about the anti-leukemia mechanisms of COX-2 inhibitor will provide a new target in clinical prevention and treatment of leukemia.

BACKGROUND:Currently, a large number of studies have confirmed that macrophage migration inhibitory factor plays an important role in a variety of biological activities, such as tumor development. In recent years, it also plays an important role in the inflammatory process, and has achieved a lot of results. However, it is unclear whether and how the macrophage migration inhibitory factor plays a role around the oral implant under oral environment.
OBJECTIVE:To investigate the effect of macrophage migration inhibitory factor on the inflammation of bone marrow mesenchymal stem cels growing around the titanium implant.
METHODS:First, bone marrow mesenchymal stem cels were seeded onto titanium cel culture disks to simulate the peri-implant environment in the mouth, and then, the cels were divided into four groups: control group, without any stimulation; lipopolysaccharide group, lipopolysaccharide-induced inflammation of bone marrow mesenchymal stem cels; non-specific smal interfering RNA (siRNA)+lipopolysaccharide group, non-specific siRNA-transfected and lipopolysaccharide-induced cels; macrophage migration inhibitory factor siRNA+lipopolysaccharide group, cels under the stimulation of lipopolysaccharide were transfected with macrophage migration inhibitory factor.
RESULTS AND CONCLUSION: Using flow cytometry, the cels expressing over 95% CD29 and CD90 as wel as less than 5% CD 45 were selected in the experiment. Cel counting kit-8 test showed that macrophage migration inhibitory factor siRNA+lipopolysaccharide had no influence on the proliferation of bone marrow mesenchymal stem cels. Lipopolysaccharide significantly stimulated the inflammatory reactions of bone marrow mesenchymal stem cels, which was 15-20 times of the control group (P < 0.01). However, compared with the lipopolysaccharide group, the levels of interleukin-1β, interluekin-6 and tumor necrosis factor-α were increased significantly after transfection with macrophage migration inhibitory factor siRNA+lipopolysaccharide stimulation (P < 0.01). These findings indicate that lipopolysaccharide can promote inflammation of bone marrow mesenchymal stem cels around the oral implant, but macrophage migration inhibitory factor siRNA can, to some extent, inhibit the occurrence of inflammation.

Objective] The mechanism of astragalus polysaccharides treatment of asthma was discussed. [Methods]Through the domestic and foreign about traditional Chinese medicine monomer astragalus polysaccharide on IL-4, IL-10 and other cytokines and inflammatory infiltrates aspects such as the Th1/Th2 balance, smooth muscle actin and airway mucus in the airway remodeling and asthma related gene research literature summary. [Results] Improve airway inflammation astragalus polysaccharides from activation and inhibition EOS EOS infiltration reduction, regulating Th1/Th2 imbalance, so Th0 cells to Th1 cell differentiation, and inhibiting the release of inflammatory cytokines and other aspects, APS can reduce airway hyper-responsiveness mitigate aspects peribronchial infiltration of eosinophils and airway smooth muscle cells induced byα-SMA expression and other high alleviate airway remodeling. [Conclusion] The influence of astragalus polysaccharide on asthma gene research will lay the basis for study of the mechanism of treatment of asthma, monomer research provides new ideas for traditional Chinese medicine.

Acupuncture Therapy ; Aged ; Female ; Humans ; Male ; Middle Aged ; Pterygium ; therapy ; Yin Deficiency ; therapy

Cardiovascular Diseases ; therapy ; Humans ; Medicine, Chinese Traditional ; Phytotherapy

Objective To investigate the prevalence of urinary incontinence among Uigurs in Xinjiang district. Methods 506 adults Uigurs (over 18 years old)in Xinjiang, 200 men and 306 women, were interviewed with questionnaire. Results The total incidence of urinary incontinence was 38.9%, 43.8% in women and 31.5% in men, P

Objective To investigate the transfection and expression of pcDNA3 1-VEGF 165 plasmid in mammalian cells. Methods pcDNA3 1-VEGF 165 plasmid was transfected into CHO cells by electroporation and the positive clones were screened by G418. The expression of VEGF 165 gene in the transfected CHO cells were detected by RT-PCR, ELISA and western blotting analysis. The biologic activities of VEGF 165 secreted by the transfected CHO cells were measured by Mile's assay and MTT method. Results G418-resistant CHO cell clones were obtained. RT-PCR, ELISA and western blotting analysis showed that there were the transcription and translation of VEGF 165 gene in anti-G418 CHO cells. The mile's assay showed that the expressed VEGF 165 could increase the vessel permeability, and MTT assay proved that it could promote the proliferation of endothelial cells. Conclusion pcDNA3 1-VEGF 165 plasmid could express VEGF 165 with biological activities in mammalian cells.

AIM:To investigate the influence of free hemoglobin on the initiation and progression of atherosclerosis and the role of CD163 in this process.METHODS:Thirty male SD rats were randomly divided into three groups:control group(group C),atherosclerosis group(group A),atherosclerosis and hemolysis group(group P).The hemolysis and atherosclerosis animal model was established.The free hemoglobin(FHb)and MDA levels in plasma,(RAAPIs)and intima area/midmembrane area(I/M)of each group were measured.The expressions of CD163 and heme oxygenase-1(HO-1)in atherosclerosis plaques in group A and P were detected and measured by means of immunohistochemistry and Western blotting.RESULTS:Compared with group C,the FHb,MDA,CD163 and HO-1 in group A and group P increased significantly(P0.05).The FHb level in plasma and the expressions of CD163 and HO-1 in atherosclerotic plaques correlated with each other(r=0.526,r=0.498,r=0.653;P

Objective To discuss the meaning and association with prognosis of Bcl-w expression in gastric carcinoma. Methods By Immunohistochemistry( SP) method was used to detect the expression of Bcl-w and MMP-2 in 100 cases of human gastric carcinoma. Results (1) Among 100 cases of stomach carcinoma, Bcl-w and MMP-2 positive expression rates were respectively 69. 0% , 77. 0% ; (2) The Bcl-w and MMP-2 expression in gastric carcinoma was positively associated with tumor size, invasive depth, histology differentiation and lymph node metastasis(P<0.05) ;(3)The expression of Bcl-w was significantly correlated with MMP-2 in gastric carcinomas (P =0.002) ; (4) Five years survival rates and overall survival of Bclw-positive patients were lower than the Bcl-w -negative patients. Conclusions (1)Bcl-w plays an important role in inhibiting apoptosis of gastric cancer and promoting gastric cancer invasion; (2) Bcl-w-positive expression patients have a bad prognosis and Bcl-w expression in gastric carcinoma can be regarded as a prognosis evaluation index.

AIM: To evaluate the therapeutic efficacy and safety of heavy silicone oil (HSO), Densiron 68, was used as internal tamponade to treat complex vitreoretinopathy.METHODS: A retrospective study of 30 patients (30 eyes) who underwent vitrectomy and HSO tamponade for complex retinal detachment between January 2015 and January 2016.The best corrected visual acuity (BCVA), intraocular pressure (IOP), retinal reattachment and complications after surgery were observed.RESULTS: There were statistical significances in both the BCVA difference between pre-operation and HSO tamponade, and the BCVA difference between pre-operation and the removal of HSO for 3mo (z=-2.198, P=0.028;z=-2.682, P=0.007).The average intraocular pressure of HSO tamponade group was 20.233±8.007mmHg, and the average intraocular pressure of pre-operation group was 16.067±4.025mmHg, showing significant difference(t=-2.913, P=0.005).Between the pre-operation group and the HSO removed group 14.933±3.423mmHg, there was no significant statistical difference in the analysis of IOP (t=2.635, P=0.430).Anatomical success was achieved in 90% of cases after the removal of HSO.Most common complications were cataract formation and oil emulsification.CONCLUSION: Densiron-68 is a safe and effective tamponade material for the treatment of complex vitreoretinopathy.However, most common complications are cataract formation and oil emulsification.So clinicians should strictly handle indications and usage during the clinical applications.