Female ; Humans ; Melanosis ; Middle Aged ; Occupational Diseases

AIM: To explore the machanism of calcined mica by comparing the difference in crystal structure and inorganic elements of raw mica and calcined ones. METHODS: Crystal structures were compared by IR spectrum and X-ray crystallography and the contents of element in the prevention were determined with ICP-AES. RESULTS: After being calcined,crystal phase transformation changed in gold mica and did not change in white mica.Both of their chemical composition changed,crystal water decreased and crystal defects increased.There were obvious differences in the contents of inorganic elements.The content in white mica was obviously more than that in gold mica.After being calcined,the value of K/Na in white mica decreased and the contents of Ca,Mg increased,while the content of Mg increased and the content of Ca decreased in gold mica. CONCLUSION: There are(actually) many differences in contents of elements and chemical structure in white and gold mica before and after being calcined,which may explain the mechanism of calcined mica.

AIM: To study the technological parameters of the enrichmentpurification of iridoid glycoside with SP825-macroporous resin and method of increasing the content of morroniside and loganin in solid production. METHODS:()The sample volume,elution concentration,volume and methods were observed and compared with those elution solvents.The enrichment of iridoid glycosides was evaluated with contents of morroniside and loganin by HPLC methods. RESULTS: The optimum process was that sample of 2 times macroporous resin was absorbed two dynamicaly with columns of SP825macroporous resin and the column was washed with 7 column volume of 50% ethanol. CONCLUSION: After being enriched,the content of morroniside and loganin is raised 6 times and reached 69.68% in solid production.Iridiod glycoside are enriched by SP825-macroporous.It is a feasible method to produce preparation.

The paper discusses the problems and inequity in town and country family-planning service by comparing the following aspects: work thinking in the service,accessibility of family-planning service,service capacity of the organizations,resource allocation.Besides,in order to satisfy the demand of rural family-planning service market and improve the equity,some suggestions,such as integrating resource and complementing advantages to implement regional family-planning program,identifying the functions of government and changing the service thinking,increasing the investment in primary health family-planning organizations and reinforcing the co-operation between health and family-planning organizations.

We have made an investigation on medica1 college students' thinking about the teaching of human parasitology,and put forward a preliminary tentative plan and suggestion to the teaching goal,teaching content,method and so on to human parasitology teaching.

Objective To discuss the possibility of prognosing the hydatidiform mole deterioration and metastasis by detecting the expression of matrix metalloproteinase 9(MMP-9)in different type of trophoblastic disease,and analyze the correlative factors of the hydatidiform mole deterioration,so as to improve the criterion of hydatidiform mole prognosis.Methods The expression of MMP-9 in 30 cases of normal human(Iess than 12 weeks)cytotrophoblast cells,32 cases of partial hydatidiform mole,93 cases of complete hydatidiform mole,30 cages of invasive mole and 5 cases of choriocarcinoma were detected by immunohistochemical streptavidin-pemxidase.The factors as the age of onset,uterine size,luteinizing cyst,β-human chorionic gonadotropin hCG of 7 cases of the hydatidiform mole deterioration were analyzed.Results The expression of MMP-9 was detected in all types of trophoblastic cells,with the development of the malignant transformation the stainning Was markedly increased in the invasion of the normal human cytotrophoblast cells(X2=23.49,P＜0.05).Conclusion The difference of MMP-9 expressin gestational trophoblasts is helpful to predict the hydatidiform mole deterioration and metastagis,the hydatidiform mole deterioration correlates with the age of onset,β-hCG,uterine size,luteinizing cyst.

Coal ; Noise, Occupational ; prevention & control ; Steel

ObjectiveTo investigate whether miR-25 targets KLF4 to regulate the growth of gastric cancer cells and lucidate the mechanism of miR-25 producing functional effects in gastric cancer cells. Methods The expression levels of miR-25 and KLF4 were detected by qRT-PCR in 35 pairs of gastric cancer tissues of patients. Target genes of miR-25 were predicted using Targetscan and verified though dual luciferase activity assay and west-ern blot. MTT assay and clone formation assay were detected after downregulated miR-25 and overexpressed KLF4 respectively. Results miR-25 was highly expressed in gastric cancer tissues with KLF4 downexpresstion. Downreg-ulated miR-25 and overexpressed KLF4 respectively can significantly reduce the proliferation of gastric cancer cells,the results were opposite when overexpression of KLF4. Conclusion miR-25 promotes gastric cancer cells proliferation by targeting KLF4.

Objective:To investigate the effect of Aspirin on the proliferation and NOS expression in astrocytoma cell line,and probe into ins mechanism.Methods:The effect of Aspirin on the growth of astrocytoma cells evaluated by MTT assay;NOS protein levels were determined by immunohistochemistry,NO and CEA concentration in the medium were determined by Griess assay and lepton catch immuning method respectively.Results:Aspirin can inhibit the growth of astrocytoma cells,induce the expression of iNOS,increase the concentration of NO in the medium. The effect of these were in a concentration dependent pattern. Moreover,Aspirin can reduce the concentration of CEA in the medium.Conclusion:Aspirin inhibit the growth of astrocytoma cell line.UP regulated iNOS expression resulting a increase of NO concentration are ascribed to mechanism of antrproliferation activity of Aspirin. CEA is a good indicator in monitoring curative effect of astrocytoma.

The community - based healthcare service is a main component of healthcare service system in China,and its development is affected by several factors including correlative ethical issues.In order to maintain a long - term and stable development of community - based healthcare service,great importance should be attached to some concerned ethical issues,such as the human - oriented service,intimacy of patients,the interests of disadvantaged group,etc.