AIM:To investigate the expression of microRNA-193b(miR-193b) in the cervical tissues, and fur-ther to explore the effect of silencing miR-193b on diamminedichloroplatinum ( DDP)-treated HeLa cell viability.METH-ODS:The expression levels of miR-193b in different cervical tissues were examined by qPCR.After transfection of miR-193b-inhibitor, the cell migration was determined by Transwell assay, the sensitivity of HeLa cells to DDP was measured by MTT assay, the protein levels of phosphate and tension homology deleted on chromsome ten ( PTEN), protein kinase B (Akt), p-Akt and p-glycoprotein(P-gp) were determined by Western blot.RESULTS:The mRNA level of miR-193b was significantly increased in the cervical cancer tissues compared with normal cervical tissues ( P<0.05) .Knockdown of miR-193b obviously inhibited migration and enhanced sensitivity to DDP of HeLa cells (P<0.05).Additionally, after transfec-tion of miR-193b-inhibitor, the expression of PTEN was increased, whereas the protein levels of p-Akt and P-gp were de-creased (P<0.05).CONCLUSION:miR-193b is highly expressed in the cervical cancer tissues.Inhibition of miR-193b augments the sensitivity to DDP of HeLa cells, at least in part, through PTEN-PI3K/Akt signaling pathway.

This paper pondered thoroughly the analysis of the mental status of earthquake victims,the preparation,operational measures and steps,and remarkable issues in post-earthquake psychological crisis intervention,in order to provide mental support for the post-earthquake reconstruction.

The quality of teaching staff is closely correlated with the development of students and educational career.Therefore,educational administrators should enhance their concepts of management,clarify requirement and quality of teaching staff under the circumstance of new curriculum reform,and explore effective approaches to enhancing the quality of teaching staff,so as to unceasingly improve the quality of teaching staff.

Phage display is a widely used gene engineering high technology.Through the display of exogenous peptides or proteins fused specific coat protein on the surface of phages,it is possible to construct proteins or peptides libraries and screen interesting proteins,peptides and antibodies successfully.Most commonly used phage display technology is phagemid/helper phage system,in which helper phages are essential for the replication and assembly of phagemid particles.In this review,in combination with the newest research dynamic status,we summarize phagemid/helper phage double-genome system.We mainly emphasized the features and mutation mechanisms of different helper phages.We also made some prospects for the future directions,in the meanwhile,we also expect that our experience can provide some help for further maturity of the technology.

Aim To investigate the effect of sulfur dioxide (SO2) on blood pressure and aortic structure of the spontaneous hypertensive rat(SHR).Methods Sixteen male SHRs at the age of 4 weeks were randomly divided into SHR control group and SHR+Na2SO3/NaHSO3 group,respectively.Another eight male WKY rats served as WKY control group.Five weeks later, the blood pressure was determined.The rat aortas were dyed with Hart's Method. The morphometric parameters including outer radius, lumen radius and the wall thickness were calculated by Leica workstation. The proliferative index (PI) of smooth muscle cells was detected by immunohistochemical assay.Results The blood pressure of SHR control increased compared with that of WKY control rats(P

Dust ; analysis ; Humans ; Occupational Exposure ; analysis ; Power Plants

Nasopharyngeal carcinoma (NPC) is prevalent in Southeast Asia, especially in the area of southern China. It is acknowl-edged that NPC is absolutely related to EBV infection. Nevertheless, there are just a few researches about the relationship between the level of EBV antibodies and the tumor stages and it hasn't come to conclusion. This review makes a summary on present research progress and would be valuable for the better understanding of the contribution of EBV antibodies to the tumor stages in NPC.

Objective To observe and evaluate the clinical efficiency and safety of topiramate for treatment of patients with various types of epilepsy Methods 142 patients suffering from epilepsy were underwent the treatment of topiramate, of them 93 cases received a to piramate add on therapy and 49 cases a monotherapy The efficiency and side effects were evaluated after 8 weeks titration period and 12 weeks stabilization period Results The total effective rate was 79% and 84% in add on group and monotherapy group respectively The seizure free rate was 28% and 41% in two groups respectively The optimal dosages were 100～200 mg/d in adults and 3～5 mg/d in children Side effects were generally mild Conclusion Topiramate is a highly effective, safe and widespread first line antiepileptic drug which can be used as a monotherapy or add on treatment

Purpose: To detect the mRNA expression of epithelial skeleton protein CK19 in the peripheral blood of patients with colorectal cancer; to study its relationship to other clinicopathological parameters; and to discuss if the detection can help post-operative adjuvant therapy. Methods: A total of 39 blood samples from colorectal cancer patients were included in the study. Specific RT-PCR primers were used to avoid the amplification of pseudogenes. Results: Of 39 patients 31 showed CK19 mRNA expression (79.5%) . The patients in Dukes stage C and D showed higher frequency of CK19 mRNA expression than that in Dukes A and B (P

Objective To measure the concentration of the amniotic fluid EPO(AFEPO) and investigate the relationship between AFEPO levels and fetal hypoxia. Methods Their AFEPO and the umbilical vein serum EPO (UVSEPO) were measured thirty pregnancy induced hypertension pregnancies (PIH group) , 30 gestational diabetic pregnancies (Diabetic group) and 25 normal pregnancies (control group) were terminated by cesarean section by using a chemiluminescent enzyme labeled immunometric assay (CELIMA). Meantime, umbilical artery pH (UapH), base excess(UaBE) and UapO2 and so on were also measured. Results The mean concentrations of AFEPO and UVSEPO in PIH (1.42?0.33 and 1.63?0.37) and Diabetic group (1.53?0.25 and 1.81? 0.34) were significantly higher than those in control group (0.91?0.11 and 1.13?0.14), ( P