At present there is no definitive therapy for food allergy and the mainstays of treatment are allergen avoidance and ready access to emergency medications. Significant progress toward an novel oral immunotherapy (OIT)for food allergy has been made. These preliminary data on OIT are encouraging, OIT can be effective in desensitizing at least a subset of children with IgE-mediated food allergy, however, it remains uncertain whether OIT can induce long-term tolerance. During OIT, allergic reactions are common, although severe reactions are less common. Additional studies are needed to realize whether tolerance would be maintained, and to determine the specific laboratory indicators in rigorous multicenter randomized and placebo-controlled trials.

Objective To observe the protective effects of ligustrazine on glutamate-induced injury in cultured hippocampal neurons. Methods Primarily cultured hippocampal neurons from fetal rats were incubated with ligustrazine (1 mmol/L) for 12 hours,then glutamate (1 mmol/L) was added for 20 minutes to induce injury. Cell viability was detected by MTT assay,and the change in LDH activity was determined by biochemical method. Finally,the expression of AchE in the cultured neurons was assayed by immuocytochemistry. Results Ligustrazine improved the survival rate of hippocampal neurons injured by glutamate,inhibited the release of LDH from the kytoplasm injured by glutamate. The expression of AchE in the injured neurons was promoted by pretreatment of ligustrazine. Conclusion Ligustrazine can significantly exert protective effects on the hippocampal neuron injury induced by glutamate.

IEEE Std 1708-2014 breaks through the traditional standards of cuff based blood pressure measuring devices and establishes a normative definition of wearable cuffless blood pressure measuring devices and the objective performance evaluation of this kind of devices. This study firstly introduces the background of the new standard. Then, the standard details will be described, and the impact of cuffless blood pressure measuring devices with the new standard on manufacturers and end users will be addressed.
Blood Pressure ; Blood Pressure Monitors ; standards ; Humans ; Reference Standards ; Telemedicine

Objective To investigate whether nestin-positive cells can be isolated from human fetal pancreases and cultured in vitro for passages. Methods The islet-like cell clusters (ICCs) were isolated from pancreases of induced human fetuses at 16, 18, 20 gestational weeks and cultured subsequently. Immunohistochemical staining and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of the nestin, pancreatic and duodenal homeobox gene-1 (PDX-1/IPF-1), islet endocrine hormone insulin and glucagon. Results Nestin-positive cells, which may further transform into sphere cell clusters that include PDX-1, insulin and glucagon expressing cells, as well as nestin-positive cells, were isolated from the ICCs in vitro and cultured with several passages. Conclusions The nestin-positive cells can be isolated from fetal pancreases, and may have the capability to proliferate and differentiate into islet endocrine cells.

Objective To assess the expression patterns of apoptosis-related genes in human pancreatic cancer cells (Patu-8988)induced by Kanglaite (KLT), a novel anti-cancer botanical product. Methods The apoptosis of Patu-8988 cells was determined by flow cytometry with Annexin V-FITC and propidium iodide staining. A human apoptosis microarray containing 96 cDNA fragments was used to detect gene expressions, and followed by Western blot analysis to confirm changes in expression of selected gene products. Results KLT induced apoptosis of Patu-8988 cells in a time-dependent manner. cDNA microarray analysis identified 5 down-regulated genes and 12 up-regulated genes (more than three fold) in the first 24 h as a consequence of treatment. Western blot performed for P53, Bcl-2, Bax and Caspase-3 were consistent with the microarray results. The enhanced activity of Caspases-3 was evidently verified by the cleavage of the 89?103 form of poly ADP-ribose polymerase. Conclusion KLT could alter the expression profile of apoptosis- related genes in human pancreatic Patu-8988 cancer cells, which provides valuable insight into the tentative signaling pathways mediating the outcome of KLT-induced apoptosis.

Objective To discuss the influence of the holistic nursing careng on clinical value in patients with advanced pancreatic cancer.Methods 70 patients with pancreatic cancer were randomly divided into the observation group and the control group,with 35 cases in each group.The control group was given routine nursing measures.The observation group received the holistic nursing careng on the basis of routine nursing measures.The hospitalization time,satisfaction degree,treatment compliance and mentality were observed.Results The SAS,SDS scores of the observation group after nursing were (56.87 ±6.01)points and (61.79 ±6.17)points,which were significantly lower than (39.11 ±5.68)points and (40.82 ±5.31)points of before nursing(t =9.136,8.561,all P <0.05).The hospi-talization time,exhaust time of the observation group were (32.9 ±6.6)days and (8.21 ±1.29)days,which were significantly lower than (45.1 ±10.2)days and (13.56 ±1.77)days of the control group(t =8.561,3.769,all P <0.05).The treatment satisfaction and treatment compliance in the observation group were also significantly better than the control group(χ2 =8.292,8.156,all P <0.05).Conclusion Hsystematic nursing intervention can significantly improve thepostoperative anxiety and depression,and improve the quality of life of patients with severe pneumonia.

Objective: To study the critical influence factors in the preparation of orally disintegrating tablets of fresh antler with all ingredients (ODT-FA) by lyophilized method and to optimize the preparation technology. Methods: The lyophilized molding technology was used to prepare the ODT-FA and the optimal formulation and preparation were obtained by means of single factor test with promoting cell proliferation activity, moisture content, disintegration time, and appearance as evaluation criterion. Results: The optimal preparation conditions of ODT-FA were as follows: The frozen storage temperature of fresh antler was -40°C, acetate buffer was used as diluent to mix with the fresh antler powder according to ratio of 1:2, then to homogenize for 10 min under liquid nitrogen protection with 10% trehalose as lyoprotectant, and the homogenate was lyophilized in vacuum after poured into the mold to prepare the ODT-FA. The tablets could disintegrate within 30 s completely and its moisture content was less than 5%. In addition, there was no difference in promoting cell proliferation compared with fresh antler. Conclusion: The ODT-FA prepared by optimal technology has highly biological activity and is easy to take. The optimal preparation process is stable and suitable for industrial production.

OBJECTIVE To determine distribution of pathogens and resistance characteristic in urinary infection and to provide basis for clinicians to select antibiotics correctly.METHODS A total of 272 strains of pathogens were identified and ESBLs-producing G-bacilli were detected.RESULTS Among 272 strains,68.4% of G-bacilli,19.1% of G+cocci,12.5% of fungi,41.1% of Escherichia coli and 18.2% of Klebsiella pneumoniae strains produced ESBLs.CONCLUSIONS G-bacilli are major pathogens of urinary infection and their resistance is raised.Much attention should be paid to resistance detection.It is important to control nosocomial infection and select antibiotics correctly.

Aged ; Humans ; Lung Diseases, Fungal ; complications ; Silicosis ; complications ; microbiology ; Tuberculosis, Pulmonary ; complications

Objective To explore effects of ginsenosides Rg1 on the expression of poly(ADP-ribose) polymerase-1 (PARP-1) and tumor necrosis factor receptor (TNFR) 1 in cortex cells after focal cerebral ischemia in rats. Methods Ninety healthy rats were randomly divided into sham-operative group, focal cerebral ischemia group, ginsenoside Rg 1groups (low, medium and high concentrations) and drug control group. Rats were intraperitoneally injected saline 45 mg/kg, saline 45 mg/kg+ginsenosides Rg1 10, 20 and 40 mg/kg, nimodipine 1 mg/kg 5 d before surgery, respectively. Focal cerebral isch?emia model was made by middle cerebral artery occluding in rats. The neurological deficit score and TTC staining were used to verify the success of the rat model. The expressions of PARP-1 and TNFR1 were evaluated by immunohistochemical meth?od and Western blot technique. Results There were obvious symptoms of neurological deficit and large pale infarct area in focal cerebral ischemia group compared with those of sham-operative group. There were higher percentages of neurological deficit score and infarct area in ginsenosides Rg1 groups and positive control group than those of sham-operative group, but which were lower than those of ischemia group (P<0.05). There were no significant differences between ginsenosides Rg1 groups and positive control group. The positive cells of PARP-1 and TNFR1 were higher in ginsenosides Rg1 low-dose group than those of sham-operative group and positive control group, while ones of medium and high-dose Rg1 group were higher than those of sham-operative group, and were lower than those of ischemia group (P<0.05). Compared with sham-op?erative group, PARP-1 and TNFR1 expression strips were significantly enhanced in ischemia group. Expression strips were higher in ginsenosides Rg1 low-dose group than those of sham-operative group. Expression strips were higher in ginsen?osides Rg1 medium-dose group than those of sham-operative group, but which were lower than those of ischemia group, and ones of high-dose group were lower than ischemia group (P<0.05). Conclusion Ginsenoside Rg1 shows protective effects on focal ischemia injury, which may be related with down-regulation of the expression of PARP-1 and TNFR1.