Objective:To study the effects of combination of arsenic trioxide and octreotide on the proliferation and apoptosis of hepatocellular carcinoma cells(HCC),and to explore the mechanism of their action on liver cancer.Methods:Heptocellular carcinoma cell SMMC-7721 was treated with arsenic trioxide and octreotide.Cell survival rate was determined by MTT assay;cell cycle and the apoptosis index were determined by flow cytometry (FCM);the morphological changes were observed by transmission electron microscope.Results:The combination of arsenic trioxide and octreotide significantly inhibited the growth of SMMC-7721 and the concentration of each drug in combination is 2.022?mol/L and 8.088?mol/L that was less than single use of arsenic trioxide (3.707?mol/L)and octreotide (22.637?mol/L).The combination of two drugs can arrest HCC in S phases and induce apoptosis.Conclusion:Arsenic trioxide can enhance the antiproliferative effect of octreotide to human HCC and induce apoptosis.

The methods for analysis of cosmetics including chemical analysis and instrument analysis, such as spectrophotometry, electrochemical analysis, chromatography (gas chromatography, liquid chromatography and thin-layer chromatography), fluorescence analysis, were summarized in this article in recent years. The prohibited and limited components in cosmetics were determined mainly by chromatography method. The methods for determination of hygienic chemical indexes of cosmetics had been mature. The methods for determination of prohibited and limited components in cosmetics had been studied a little, so should be strengthened more.

Objective To investigate the clinical characteristics and prognosis of young patients with endometrial cancer.Methods Thirty cases with endometrial cancer were selected as our observed group (age less than 40 years old).Forty cases with endometrial cancer were selected as control group and their age were over 40 years old.The information about clinical characteristics,diagnosis,treatment and prognosis of patients in two groups were collected.Results The rates of primary infertility and less than 3 times of pregnancy in observed group were 30.0％ (9/30),56.7％ (17/30) respectively,higher than that in control group (5.0％(2/40) or 30.0％ (12/40)).The differences were statistically significant (x2 =6.183,5.372 respectively,P ＜ 0.05).The positive rate of estrogen receptor(ER) and progesterone receptor(PR) expression were 93.3％ (28/30) and 86.7％ (26/30) in observed group,higher than that in control(42.5％ (17/40),52.5％ (21/40)respectively).The differences were statistically significant (x2 =5.951,4.721 respectively,P ＜ 0.05).Two groups of patients were statistically significant differences in pathological stage,the pathological type of observed group was mainly stage Ⅰ (76.7％ (23/30)),higher than that of control group (47.5 (19/40),and the difference was statistically significant (x2 =5.464,P ＜ 0.05).There was statistically significant difference between the observed and control group on Cervical infiltration and lymph node metastasis(x2 =5.464,1.018respectively,P ＜ 0.05).Conclusion The infeaility and less than 3 times of pregnancy may be the risk factors of younger endometrial cancer and the pathological type of observed group was mainly stage Ⅰ.However the prognosis is similar between the young and the old patients.

Objective To investigate the chnical effect of levonorgestrel-releasing intrauterine system(LNG-IUS) in patients with adenomyosis.Methods A total of eighty patients with adenomyosis were enrolled and underwent LNG-IUS insertion.The degree of dysmenorrhea,amount of menorrhea,size of the uterus,endometrial thickness,hemoglobin as well as hormonal changes and adverse reaction before treatment and after treatment for 3,6,12 months were compared.Results After treatment for3,6,12months,the amount of menorrhea was reduced [(52.4 ± 8.4)％,(22.6 ± 9.8)％,(14.8 ± 8.6)％ vs.(100.0 ±0.0)％],the scores of dysmenorrhea was reduced [(1.2 ± 0.5),(0.8 ± 0.4),(0.3 ± 0.2) scores vs.(2.4 ±0.6) scores],the level of hemoglobin was increased [(104.4 ± 11.4),(109.2 ± 12.6),(118.8 ± 13.4) g/L vs.(88.6 ± 16.5) g/L],the endometrial thickness was thinner [(6.3 ± 1.4),(3.6 ± 0.8),(1.8 ± 0.5) mm vs.(9.2 ± 2.8) mm],the size of the uterus was reduced [(156.8 ± 56.8),(142.4 ± 48.9),(126.6 ± 36.5)cm3 vs.(172.6 ± 62.5) cm3],there was significant difference compared with that before treatment (P ＜ 0.05).After treatment for 3,6,12 months,the levels of folhcle-stimulating hormone,luteinizing hormone and estradiol had no significant difference compared with those before treatment (P ＞ 0.05).After treatment for 3,6,12 months,the level of cancer antigen 125 was significantly reduced compared with that before treatment [(34.6 ± 11.6),(16.6 ± 9.8),(11.7 ± 6.4) kU/L vs.(62.4 ± 24.3) kU/L].Conclusiom The LNG-IUS has exact therapeutic effect in treatment of adenomyosis,and has little influence on ovarian function.It is worth of using in clinic.

Objective To explore the clinical efficacy of ethinylestradiol and cyproterone acetate (Diane-35) combined with mefformin in patients with polycystic ovary syndrome (PCOS).Methods One hundred and sixty patients with PCOS were divided into control group and observation group by random digits table method with 80 cases each.Patients in the control group were given Diane-35 only,while patients in the observation group were given Diane-35 combined with metformin.The triglycerides (TG),total cholesterol (TC),low density lipoprotein cholesterol (LDL-C),fasting insulin (FINS),fasting blood glucose (FBG),follicle-stimulating hormone (FSH),luteinizing hormone (LH) and testosterone (T) of patients in the two groups were measured before treatment and after treatment 6 months,the rates of pregnancy and abortion were recorded.Results TC,LDL-C,FINS,FBG,FSH,LHt and T of both the 2 groups after treatment decreased obviously than before treatment,and TG in observation group decreased obviously than before treatment,there were statistical differences (P ＜ 0.05).There was no statistical difference in TG in control group between before treatment and after treatment (P ＞ 0.05).After treatment,TG,FINS,FBG,LH in observation group were lower than those in control group [(1.0 ± 0.2) mmol/L vs.(1.6 ± 0.8) mmol/L,(15.2 ± 6.3) mU/L vs.(18.9 ± 6.2) mU/L,(4.5 ± 1.1) mmol/L vs.(5.1 ± 0.9) mmol/L,(6.3 ± 3.0) U/L vs.(7.4 ± 2.9) U/L],there were statistical differences (P ＜ 0.05).After treatment,the rate of pregnancy in observation group was higher than that in control group [61.2％(49/80) vs.42.5％(34/80)],there was statistical difference (x2 =5.633,P＜ 0.05).The rote of abortion in observation group was lower than that in control group [6.1％(3/49) vs.17.6％(6/34)],but there was no statistical difference (x2 =1.694,P ＞ 0.05).Conclusion The clinical effect of Diane-35 combined with metformin in patients with PCOS is better than Diane-35 only.

Objective:To study the changes of cell cycle phase and cell cycle phase regulation protein during arsenic trioxide induced apoptosis in hepatoma cells,and to explore mechanisms of arsenic trioxide inhibited HCC.Methods:Cultured in vitre,HCC SMMC-7721 were treated 72h with 2?mol/L arsenic trioxide.The cell cycle and apoptosis index were detected by flow cytometry(FCM);the morphological changes were observed by transmission electron microscope.The immunohistochemistry was used to detect the protein expression of p21、cyclin D_1,cyclinA.Results:SMMC-7721 cell cycle was arrested in G_1 and S phase by arsenic trioxide.Compared with control in the course of apoptosis induced by arsenic trioxide p21 protein expressions rose significantly(control:1.128;arsenic trioxide:3.794),while cyclinD_1,cyclinA protein expressions decreased significantly(control 1:3.647,2.833;arsenic trioxide:1.133,1.179).Conclusion:Arsenic trioxide could disturb cell cycle progression of SMMC-7721 and induce apoptosis.The mechanism may relate to that arsenic trioxide up-regulate expression of p21 and down-regulate expression of cyclinD_1,cyclinA.

0. 05). Conclusion :Cordyceps sinensis can inhibit the hepatic fibrosis,and at the same time,doesn't improve the hepatic glycogen synthesis.

Actins ; metabolism ; Adult ; Breast Neoplasms ; metabolism ; pathology ; surgery ; Diagnosis, Differential ; Female ; Humans ; Immunohistochemistry ; Middle Aged ; Neoplasm Recurrence, Local ; Phyllodes Tumor ; metabolism ; pathology ; surgery ; Vimentin ; metabolism

Early diagnosis of prediabetic peripheral neuropathy depends on the evaluation of small fibers.Traditional nerve conduction tests can only evaluate the function of large myelinated fibers,while lack of sensitivity to small fibrous lesions.These lesions were related to pain and autonomic neuropathy.In recent years,with the progress of neurophysiological diagnosis technology,the early diagnosis of diabetic peripheral neuropathy has been improved.Clinical methods,commonly used in the detection of small fibrous lesions,mainly include skin sympathetic response,quantitative sensory test,contact heat pain evoked potential,and quantitative sudomotor axonal reflex test.In this paper,the pathogenesis of diabetic peripheral neuropathy,neurological pathological changes and applications of electrophysiology technology were reviewed to provide an objective basis for early diagnosis of prediabetic peripheral neuropathy.

Objective To study the anti acute rejection effect of ethanol extract of poria cocos wolf (EEPCW) in heart transplantation rats.Methods Homologous heart transplanted rats were divided into 4 groups ( n =20 in each group). EEPCW 50?mg?kg -1 ?d -1 , EEPCW 25?mg?kg -1 ?d -1 , CsA 5?mg?kg -1 ?d -1 and olive oil 8?ml?kg -1 ?d -1 were given to different groups. Cardiac allograft survival was observed on 10 of each groups. The pathologic lesion, the contents of IL 2 and IFN ? in peripheral blood, percentage of CD3 +, CD4 +, CD8 + lymphocytes and ratio of CD4 +/CD8 + were determined on another 10 in each groups and 10 normal rats. Results Survival of the donor heart was significantly prolonged and pathologic lesion was relieved, the contents of IL 2 and IFN ? in peripheral blood, percentage of CD3 +, CD4 +, CD8 + lymphocytes and ratio of CD4 +/CD8 + were significantly reduced in EEPCW groups.Conclusion Acute rejection of heart transplantation can be effectively suppressed by ethanol extract of poria cocos wolf.