1.Developing of the automatic grinding machine for dentures
Jingtao CHENG ; Tianwen GUO ; Puquan XI
Journal of Practical Stomatology 2000;0(05):-
Objective: To develop a new denture grinding machine to replace the manually grinding procedure for metallic denture parts. Methods: Two hexagonal barrels were symmetrically fixed on a round plate. The direction of rotation and revolution of the barrels were reversed. The denture parts that were put into the barrels together with grinding materials and solution were ground by centrifugal force caused by rotation and revolution of the barrels. Results: The surface roughness of pure titanium castings went down from 1.060 ?m to 0.3113 ?m after ground with the automatic grinding machine using different shapes of grinding materials in series. Conclusion: The automatic grinding machine is competent for grinding the metallic denture parts.
2.A method to predict clustered repeats in Salmonella genomes
Xi CHENG ; Zhirong GUO ; Yueming HU ; Yejun WANG
Basic & Clinical Medicine 2017;37(2):150-155
Objective To develop a simple method identifying and illustrating clustered repeats in bacterial ge-nomes, and to observe the patterns of clustered repeats in Salmonella genomes.Methods Bacterial genomes were cut to be overlapped pieces of identical size with a sliding window strategy .Each piece of genome fragment was aligned against itself with BLAT integrated in PipMaker , which was further used to build collinearity figures . Collinearity figures were analyzed to identify the clustered repeats.Results With the new pipeline CRpred ( Clustered Repeat Predicter) , Salmonella typhimurium LT2 genome was screened, and in 151 clustered repeats were disclosed.Pattern analysis on these repeats indicated that there were five categories, including low-copy simple tandem repeats, high-copy simple tandem repeats, interspaced tandem repeats, reverse-complementary re-peats, and interspaced reverse-complementary repeats.Nine repeat regions in LT2 genome were discovered which could not be simply classified into the 5 categories defined above.Conclusions A new, simple and intuitive strategy is proposed to identify and show clustered repeats in genomes , providing clues for CRISPR , VNTR and oth-er repeat-related studies .
3.Comparative genomic analysis of Salmonella type Ⅲsecreted candidate gene yiiG
Xi CHENG ; Yueming HU ; Zhirong GUO ; Yejun WANG
Basic & Clinical Medicine 2017;37(1):43-49
Objective To study the distribution and evolution of yiiG, a Salmonella gene encoding a candidate type secretedsubstrate .Methods Salmonella genomes were comprehensively screened for yiiG distribution with se-quence alignment strategies .The evolutionary history of yiiG was traced .Comparative genomic analysis was per-formed to study the evolutionary mechanisms of yiiG gene acquisition , loss and duplication .RNA-seq data were combined to analyzing the correlation between yiiG and other virulence factors .A variety of bioinformatic tools were used for discovering the possible type Ⅲsecretion signals .Results yiiG distributed in S.enterica subsp.enterica but variable in other subspecies of S.enterica.No yiiG was found in S.bongori.Besides Salmonella, only a part of Shigella and E.coli strains were detected with yiiG homologs .The genomic locus of yiiG and its adjacency showed conservation among all Salmonella, E.coli and Shigella strains.In most of the serovars of S.enterica subsp.enteri-ca, there was a head-to-head tandem whole yiiG repeat sequence upstream the yiiG gene, which was renamed as yiiGRrc.RNA-seq analysis showed that yiiG gene expression level was highly correlated with T 3SS-related genes . Bioinformatic prediction also indicated the T 3SS effector signals in YiiG N-terminus.Conclusions yiiG represented an ancient genomic locus , which will be a hot spot where rearrangement events frequently happened .The function of yiiG could potentially be related with Salmonella virulence.Finally, a new protein-encoding gene (yiiGRrc) was newly identified that was closely related with yiiG, providing the target for further understanding the composition , function and function variation of yiiG gene family .
4.Effect of antimicrobial use density on antimicrobial resistance rate of healthcare-associated Staphylococcus aureus half a year later
Defang GUO ; Changhui CHENG ; Chunjing FU ; Zhewei CAO ; Zulian XI
Chinese Journal of Infection Control 2017;16(1):36-40
Objective To explore the effect of antimicrobial use density (AUD) on the detection rate of methicillin-resistant Staphylococcus aureus (MRSA) and antimicrobial resistance rate of healthcare-associated Staphylococcus aureus (HA-SA) half a year later.Methods From 2012 to 2015,all types of AUD,detection rate of MRSA,and antimicrobial resistance rate of HA-SA were calculated semiannually,correlation between antimicrohial resistance rate of HA-SA and all types of AUD in the same first half of year were analyzed with correlation analysis and multiple linear regression.Results From the first half of 2012 to the latter half of 2015,the total AUD declined from 128.2 to 49.0,except the AUD of carbapenems rose,AUD of other antimicrobial agents declined.From the latter half of 2012 to the latter half of 2015,104 249 patients were admitted to the hospital,and 1 008 strains of SA were isolated from 40 884 specimens,857 (85.02%) of which were community-associated SA(CA SA) and 151 (14.98%) were HA-SA.Isolation rate of HA-MRSA declined from 31.25% in the latter half of 2012 to 12.50% in the latter half of 2015;isolation rate of CA-MRSA rose from 7.08% to 16.08%,resistance rate of HA-SA was generally higher than that of CA-SA.Antimicrobial resistance rate of HA-SA to ciprofloxacin remained the same,to levofloxacin increased,to 8 other antimicrobial agents all declined;resistance rates of CA-SA to oxacillin,ciprofloxacin,clindamycin,gentamicin,and levofloxacin increased,but to other antimicrobial agents declined;no SA strains was found to be resistant to vancomycin and linezolid.The resistance rate of HA-SA to azithromycin and erythrocin was correlated with the AUD of macrolides,resistance rate of HA-SA to clindamycin was correlated wvith the AUD of aminoglycosides,to gentamicin was correlated with the AUD of macrolides and the total AUD.Conclusion The selective pressure of antimicrobial agents is still the important cause of the occurrence of antimicrobial resistance,decreasing the AUD of antimicrobial agents will help for reducing the detection rate of HA-MRSA and drug resistance rate of HA-SA.
6.Molluscicidal experiment of endophytes from Pseudolarix kaempferi Gord
Shangbin GUO ; Jun CHEN ; Jia HE ; Xi CHENG ; Nan ZHONG
Chinese Journal of Schistosomiasis Control 1989;0(04):-
Objective To screen 18 endophytes from Pseudolarix kaempferi Gord for molluscicidal effect and identify them by morphology. Methods Molluscicidal tests were performed according to the immersion test suggested by WHO and the strain screened was identified by the slide culture. Results The mortality rates of snails immersed by JJ18 broth salified (pH=7) were 26.7%, 76.7% and 100.0% for 24,48 h and 72 h, respectively, and 53.3% and 86.7% in 5% and 10% concentrations of JJ18 broth, respectively. The active components were extracellular moiety of the broth which had no acute toxicity to fish, and JJ18 strain belonged to Aspergillus. Conclusion Extracellular moiety of endophyte JJ18 from Pseudolarix kaempferi Gord is a new resource of molluscicide.
7.MRI diagnosis of cavernous transformation of the portal vein
Ting-zhen GUO ; Li WANG ; Xi-cheng GUO ; Xiao-jie ZHANG ; Yu-quan GAO ;
Chinese Journal of Postgraduates of Medicine 2012;35(9):23-26
ObjectiveTo investigate the value of MRI in the diagnosis of cavernous transformation of the portal vein (CTPV).MethodsPlain MRI,dynamic enhanced and (or) dynamic contrast enhanced magnetic resonance angiography(DCE-MRA) findings in 30 patients of clinical-proved CTPV were retrospectively analyzed.ResultsAmong 30 CTPV patients on plain MRI,obliteration of main and (or) branched portal vein were found,and mass-like or reticular abnormal soft-tissue signals were around the vein,which were produced by collateral vessels.On dynamic enhanced MRI,abnormal hepatic perfusion during arterial phase and abnormal enhanced collateral veins during portal phase could be seen.The above signs became more obvious on DCE-MRA.ConclusionsMRI and DCE-MRA can clearly visualize the anatomical features of CTPV.It is important and can provide the reliable evidence for planning properly therapeutic protocol to recognize and directly evaluate the CTPV.
8.Clinical efficacy evaluation of needle-knife for lumber disc herniation based on surface electromyography signals.
Xi-Yun YANG ; Zhi-Ru CHEN ; Da-Cheng ZHAO ; Jian GUO
Chinese Acupuncture & Moxibustion 2014;34(8):798-800
OBJECTIVETo apply needle-knife to treat lumber disc herniation (LDH) and surface electromyography were used to analyze biomechanical characteristic of patient's lumber muscle to make a comprehensive evaluation on its efficacy.
METHODSThirty patients who met the inclusive criteria were selected and treated with needle-knife, once a week for 2 weeks. Visual analogue scale (VAS), ASLR and JOA score before and after treatment were observed. Surface electromyography was applied to test the surface electromyography signals. AEMG, MFs and MPF were calculated before and after the treatment.
RESULTSAfter treatment, VAS was significantly reduced, ASLR, JOA, AEMG and MPF were obviously increased, and the absolute value of MFs was lowed (all P < 0.01).
CONCLUSIONThe needle-knife could significantly relieve lumbar muscle strength, muscle tone and muscle fatigue, improve in the imbalance of lumbar extensor muscle group, leading to the recovery of biomechanical characteristic, and the clinical efficacy is superior.
Acupuncture Therapy ; Adult ; Electromyography ; Female ; Humans ; Intervertebral Disc Displacement ; diagnosis ; physiopathology ; therapy ; Lumbar Vertebrae ; physiopathology ; Male ; Middle Aged
9.Treatment of 41 patients with advanced stage of nasopharyngeal carcinoma by combination therapy of radiotherapy and Chinese herbal drugs for activating blood circulation to remove stasis as hirudo.
Guang-wu HUANG ; Cheng-xi XIE ; Guo-qian KUANG
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(10):777-778
Adult
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Aged
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Animals
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Antineoplastic Agents
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therapeutic use
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Combined Modality Therapy
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Drugs, Chinese Herbal
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therapeutic use
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Female
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Follow-Up Studies
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Humans
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Leeches
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Lymphatic Metastasis
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Male
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Materia Medica
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Middle Aged
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Nasopharyngeal Neoplasms
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drug therapy
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pathology
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radiotherapy
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Phytotherapy
10.Commonality and pattern analysis of acupoint selection in moxibustion treatment of asthma
Guo-Shan ZHANG ; Ran-Ran QIU ; Jiang PAN ; Jian ZHANG ; Chi ZHANG ; Cheng-Xi WANG
Journal of Acupuncture and Tuina Science 2019;17(6):451-456
Objective:To analyze and summarize the commonalities and patterns in acupoint selection for moxibustion treatment of asthma. Methods: Data retrieval was conducted using ‘moxibustion’ and ‘asthma’ as the keywords through China National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full-text Database (Wanfang) and Chongqing VIP Database (CQVIP). Excel 2010 was used to establish the major acupoint database for moxibustion prescriptions in treatment of asthma; data mining methods including association patterns and clustering were adopted to analyze the characteristics and patterns in acupoint selection for moxibustion treatment of asthma. Results: A total of 161 moxibustion prescriptions were recruited. The most commonly used acupoint was Feishu (BL 13), the most commonly used meridian was the Bladder Meridian of Foot Taiyang, and the most commonly treated region was the back. The association rule analysis showed that Feishu (BL 13)-Dazhui (GV 14)-Fengmen (BL 12) had the most significant correlation, and the clustering analysis discovered 5 effective acupoint clusters. Conclusion: In moxibustion treatment of asthma, topical acupoints Feishu (BL 13), Dazhui (GV 14) and Fengmen (BL 12) can be selected, along with the acupoints from the Bladder Meridian of Foot Taiyang and the back.