1.Review on the development and application of electroacupuncture apparatus in the past 5 years in China
Yu-Yun WEI ; Qiang XI ; Yi GUO
Journal of Acupuncture and Tuina Science 2019;17(4):284-288
Objective: To review the problems existing in the development and application of current electroacupuncture (EA) apparatuses, and provide reference for the development of EA apparatuses. Methods: Related literatures about EA apparatus were derived from China National Knowledge Infrastructure (CNKI), Wanfang Academic Journal Full-text Database (Wanfang) and Chongqing VIP Database (CQVIP) between 2013 and 2017. Literatures about the development of EA apparatuses and the application for experimental research and clinical treatment published in Chinese journals were retrieved, and the development and application of EA apparatuses were summarized. Results: Among the 107 selected literatures, 48 types of EA apparatuses were used, of which only 11 models could be found in the registration list and within the validity period. There was no registration information for the EA apparatus newly developed in the past 5 years. The EA parameters used in experimental research and clinical treatment in Chinese medicine were different, and the combination of stimulation parameters was diverse. Conclusion: At present, most of the new EA products are still in the experimental or research stage, and the transformation of results has not yet been achieved. Moreover, there is no unified standard for the use of EA apparatus and its stimulation parameters in experimental research and clinical treatment, which is not conducive to the standardization process of EA stimulation.
2.Analysis of the Efficacy and Safety of L-Glutamine Granules in Treating Severe Burn Patients
Xi PENG ; Zhongyi YOU ; Fengjun WANG ; Linghui TAO ; Yun ZHAO ; Guangzhao HE ; Lei GUO
China Pharmacy 2001;12(6):358-359
OBJECTIVE: To evaluate the clinical therapeutic effect of L-glutamine granules on intestinal damage of severe burn patients and the safty of the drug.METHODS: Thirty-nine severe burn patients were randomly divided into two groups: control group(C group, nineteen patients) and L-glutamine treatment group(GLN group, twenty patients) .GLN group patients were given L-glutamine in a dose of 30g per day for 7 days, and C group patients were given the same dosage of placebo for 7 days.The plasma L-glutamine concentration, the degree of intestinal mucosa damage, blood biochemistry and complication were observed and wound healing rate of burn area was determined, then the length of hospital stay was recorded.RESULTS: After 7 days of taking L-glutamine orally, plasma L-glutamine concentration in GLN group was significant higher than that in C group(P<). The degree of intestine damage and intestinal mucosal permeability in GLN group were lower than those in C group. In addition, the wound healing rate was faster and the length of hospital stay was shorter in GLN group than those in C group. CONCLUSION: Administration of L-glutamine could abate the degree of intestine damage obviously, lessen intestinal mucosal permeability, ameliorate wound healing rate and reduce the length of hospital stay.
3.Hospital Infection in EICU Ward: Investigation and Analysis
Xiao-Li SONG ; Ying HOU ; Hui LV ; Xi-Yun GUO ; Xiao-Qiu YANG ;
Chinese Journal of Nosocomiology 2006;0(06):-
OBJECTIVE To investigate the hospital infection rate and sites of patients in emergency intensive care(unit)(EICU),and provide basis for prevention and treatment of hospital infection.METHODS Patients who were hospitalized in EICU were investigated by retrospective study.RESULTS Among the hospital infection sites,(respiratory) tract was the most frequent one(65.63%),the next was urinary tract(28.13%),and the deep vein was the third(6.25%).The most common hospital infection bacteria were Staphylococcus aureus,Pseudomonas aeruginosa and Acinetobacter baumannii.CONCLUSIONS The analysis of the subjective and objective factors of hospital infection,and the acknowledge of relationship between nursing and hospital infection sites are(important) basis for hospital infection prevention and treatment.
4.Clinical efficacy evaluation of needle-knife for lumber disc herniation based on surface electromyography signals.
Xi-Yun YANG ; Zhi-Ru CHEN ; Da-Cheng ZHAO ; Jian GUO
Chinese Acupuncture & Moxibustion 2014;34(8):798-800
OBJECTIVETo apply needle-knife to treat lumber disc herniation (LDH) and surface electromyography were used to analyze biomechanical characteristic of patient's lumber muscle to make a comprehensive evaluation on its efficacy.
METHODSThirty patients who met the inclusive criteria were selected and treated with needle-knife, once a week for 2 weeks. Visual analogue scale (VAS), ASLR and JOA score before and after treatment were observed. Surface electromyography was applied to test the surface electromyography signals. AEMG, MFs and MPF were calculated before and after the treatment.
RESULTSAfter treatment, VAS was significantly reduced, ASLR, JOA, AEMG and MPF were obviously increased, and the absolute value of MFs was lowed (all P < 0.01).
CONCLUSIONThe needle-knife could significantly relieve lumbar muscle strength, muscle tone and muscle fatigue, improve in the imbalance of lumbar extensor muscle group, leading to the recovery of biomechanical characteristic, and the clinical efficacy is superior.
Acupuncture Therapy ; Adult ; Electromyography ; Female ; Humans ; Intervertebral Disc Displacement ; diagnosis ; physiopathology ; therapy ; Lumbar Vertebrae ; physiopathology ; Male ; Middle Aged
5.Expression of hepatitis B virus e gene synthesized in Pichia pastoris and the immunological characterization of the product
Zhao-Xia LI ; Yun XI ; Guo-Qiang HONG ; Bo HU ; Min-Jian HANG ; Jue XU ; Lin LI ;
Chinese Journal of Laboratory Medicine 2003;0(09):-
Objectives To synthesize hepatitis B virus e gene which was suitable for yeast protein expression and express hepatitis B virus e antigen (HBeAg) in Pichia pastoris.Methods Using synonymous codons preferred by yeast usage on protein expression to replace some native codons of wild-type HBV e gene,the synthetic e gene (syneAg-gene) was achieved by a recursive PCR (rPCR).The syneAg- gene was inserted into the yeast expression vector pPICZ?A.The recombinant plasmid was transformed into GS115 yeast by electroporation.The yeast transformant induced by methanol expressed the HBeAg.Results The restriction analysis and DNA sequencing confirmed that the syneAg-gene was inserted to yeast pPICZ?A in correct orientation.SDS-PAGE,immunoblot and ELISA indicated that the secreted form of HBeAg was expressed by the yeast transformant.The expression level of HBeAg in the strain containing syneAg-gene was 63 mg/L.The titer of the recombinant HBeAg in culture superuatant was 1 :81 920 and the maximum OD (optical density) value of absorbance was 2.8 by ELISA.No cross reactivity between Pichia pastoris-derived HBeAg and anti-HBc antibody was found.Conclusion The recombinant HBeAg with high degree of specificity and immunoreactivity is expressed efficiently in Pichia pastoris.
6.Establishment of network laboratory for blood cell analysis and its quality assurance
Wen-Juan WANG ; Pei-Pei WANG ; Bu-Yun ZHONG ; Yu CHEN ; Xi-Chao GUO ; Sheng-Hang JIN ; Wei-Min ZHANG ;
Chinese Journal of Laboratory Medicine 2003;0(09):-
Objective To establish a network laboratory for blood cell analysis and better calibrate haematology analyzers in local lab.Methods According to GB/T 15481《General requirements for the competence testing and calibration laboratories》(idt ISO/IEC 17025),we established a network laboratory providing traceability for blood cell analysis.Complete blood count was traced to Calibration Laboratory in NCCL;The secondary standard haematology analyzer with the same model and calibrator with same lot number were used for verification for a long period.Fresh blood from healthy people was used to calibrate haematology analyzers.Results Gradually we have improved our laboratory quality management system, precision as well as accuracy,which was satisfactory.The unified blood sample was adopted to calibrate different equipments in our hospital and showed consistence when compared with calibration analyzer.The correlation coefficient of all tests is more than 0.99.The relative deviation of WBC,RBC,HCT,HGB and PLT are within?7%,?3.5%,?4%,?3% and?15%,respectively.Conclusions Secondary standard systems provides good comparable results with calibration laboratory.Its tracing mode and quality control scheme could ensure the traceability and accuracy of completed blood count.Furthermore,using elective fresh blood from healthy people,the comparable results from different analyzers were achievable.
7.The effect of Astragalus membranaceus (Fisch.) Bge. on intestinal function and gut microbiota homeostasis in aging mice
Jia-ting YIN ; Shu-hui YANG ; Cheng-xi LI ; Yun LIU ; Yu-meng WANG ; Jin-ao DUAN ; Jian-ming GUO
Acta Pharmaceutica Sinica 2022;57(12):3535-3545
Aging can cause degenerative changes in the function of multiple tissues and organs in the body. Gastrointestinal diseases and intestinal dysfunction are very common in the elderly people. The purpose of this study is to explore the effect of the total extract of
8.Regulatory effect of Flos Abelmoschus manihot in mice with inflammatory bowel disease based on gut microbiota sequencing and untargeted lipidomics
Shu-hui YANG ; Cheng-xi LI ; Jian-ping LI ; Yu-meng WANG ; Yun LIU ; Jin-ao DUAN ; Jian-ming GUO
Acta Pharmaceutica Sinica 2022;57(12):3546-3556
In this study, the ameliorative effects of
9.Correlation of daily living activities with location and severity of traumatic brain injury.
Yun-ge ZHANG ; Chun-xiao LI ; Guo-fu GUAN ; Xing-rong CHEN ; Hui-min FANG ; Zhou WANG ; Xi-ping CHEN
Journal of Forensic Medicine 2014;30(6):434-436
OBJECTIVE:
To study the correlation of daily living activities with location and severity of trau- matic brain injury (TBI) and to provide a theoretical basis for improving the accuracy of expert opinion.
METHODS:
Five hundred and one cases of patients with TBI were selected. Detailed records included following: pre-injury situation, location and severity of injury, treatment and education. Daily living activi- ties scale (Barthel index) was applied to test the subjects' daily living activities. The relevance among location and severity of TBI and Barthel index was statistically analyzed.
RESULTS:
In mild TBI group, there was no significant difference in Barthel index among each location (P>0.05). In moderate TBI group, there were significant differences in Barthel index between subarachnoid hemorrhage and cerebral lobe injury, also between parietal, occipital lobes injury and frontal lobe injury, parietal, occipital lobes injury and temporal lobe (P<0.05), respectively, whereas no significant difference in Barthel index between frontal lobe injury and temporal lobe injury (P>0.05). In severe TBI, there were significant differences in Barthel index between every two different locations (P<0.05).
CONCLUSION
There is some correlation between the location of TBI and Barthel index, which provides an important reference value for analyzing and determining daily living activities after TBI.
Activities of Daily Living
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Adult
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Brain Injuries/rehabilitation*
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Female
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Humans
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Male
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Outcome Assessment, Health Care
;
Trauma Severity Indices
10.Anxiety and Related Factors of Elderly Outpatients
Xi-yun GUO ; Ying-chen LIU ; Li-ming ZHAO ; Suqin REN ; Xiaoqing JIA ; Qu KONG ; Meiling ZHOU
Chinese Journal of Rehabilitation Theory and Practice 2006;12(5):457-458
ObjectiveTo investigate the anxiety and its interrelated factors of elderly outpatients. MethodsZung self-rating anxiety scale was used to evaluate 971 elderly outpatients, the scores between different sex, age, degree of education, etc. were compared. ResultsThe scores may become significantly different with the patients' sex, age, degree of education, satisfaction in regards to medical service and recognition of the disease(P<0.05).ConclusionFactors such as sex, age, degree of education, satisfaction of medical service, etc, deeply affect the anxiety in elderly outpatients.