After an analysis of the urgent need of systematic medical information service in village and township hospitals and doctors and the advantageous conditions for medical academic libraries to provide information service for them, its imple-mentation strategies were proposed and how to promote the construction of libraries and the development of medical cause in villages and townships by providing information service in countryside were pointed out.

Genotype ; Hepatitis B, Chronic ; complications ; Hepatitis C, Chronic ; complications ; Humans ; Parvoviridae Infections ; complications ; virology ; Parvovirus ; classification ; genetics ; isolation & purification ; Parvovirus B19, Human ; isolation & purification

Setting Chinese as preparatory courses is an effective way to harmonize Chinese language teaching and medical teaching.By this way,some low-level students can be disqualified from further study,thus both the level of Chinese teaching and medical professional teaching can be improved.In the process of the eductional system reform from 5 years to 6 years,building Chinese training based preparatory course,will consummate the oversea medical undergrade education system.

Objective To observe the clinical effects of surgical treatment of retinal detachment(RD) caused by macular hole(MH) in high myopia. Methods The clinical materials of 149 eyes of 149 high myopia patients with RD caused by MH were reviewed. The cases were divided into complete posterior vitreous detachment (PVD) group and incomplete PVD group. The anatomic successful rate of operative treatment was evaluated according to the applications of vitrectomy surgery and non vitrectomy surgery respectively in each group. The visual acuity changes after the operations were also observed. Results The anatomic successful rates were as follow: 77.9% in total cases with vitrectomy surgery and 25.9% with non vitrectomy surgery ( P 0.05). Conclusions The scleral buckling combined with vitrectomy, gas intraocular tamponade and postoperative photocoagulation is an effective and optimal procedure for RD caused by MH in high myopia.

Objective:Shenkang was prescribed and prepared by our own hospital and its clinical effect and side effect for treating latent glomerulonephritis(LGN) were observed. Methods: Shenkang was composed of dog's penis Carapar Trionycis、nephritis herb and so on and prepared by the modern pharmaceutical technology. 60 cases with LGN were divided into the control group(30 cases) and the Shenkang group(30 cases) at random. Patients with protein uria in the control group were treated with the tablet of multiglycoside of Radix Tripterygii Wilfordi and patients with accompanied symptom were treated by the supportive expectant treatment. Patients in the Shenkang Capsule group were treated with it, 5 tables each time, 3 times a day and patients with accompanied symptom were also treated by the supportive expectant treatment. The treatment courses of two groups were all 3 months. 24 hour protein uria quantity、 serum total protein(TP)、 albumin(Al)、 1 hour urine red blood cell excreting rate(1huRBCer)、 serum creatinine and sodium before and after the treatment were observed. Results: Shenkang Capsule can significantly lower protein uria of LGN so as to raise the levels of TP and Al and reduce 1huRBCer. There was no side effect in the course of using Shenkang Capsule. Conclusion: Shenkang Capsule is effective for LGN.

OBJECTIVE: To investigate the effect of genistein on cell proliferation and apoptosis in human laryngeal carcinoma cell line Hep-2. METHOD: Cell Counting Kit-8 (CCK-8) assay was used to measure the 50% inhibiting concentration (IC50) value of genistein; cell apoptosis rate and the distribution changes of cell cycle were determined with flow cytometry assay after treatment by gensitein. The morphological changes of tumor cells were evaluated by inverted phase contrast mircroscopy. RESULT: The IC50 of geniste responses to Hep-2 cells for 24 h was 23.64 µg/ml. The apoptotic rates of Hep-2 cells treated by genistein for 24 h were 22.40% ± 1. 65% (at 12 µg/ml genistein) and 30.64% ± 2.94% (at 24 µg/ml genistein) respectively, significantly statistical differences were foundbetween above threated groups and the control group (P < 0.05); the apoptotic rates of Hep-2 cells treated by genistein for 48 h were 30.55% ± 0.72%(at 12 µg/ml genistein) and 48.69% ± 1.06% (at 24 µg/ml genistein) respectively, significantly statistical differences were found between above threated groups and the control group (P < 0.05). When Hep-2 cells exposed to the same concentration of genistein for 24 h, 48 h respectively, the difference in apoptotic rate was statistically significant. CONCLUSION Genistein inhibited Hep-2 cells growth obviously, meanwhile it could induced apoptosis of Hep-2 cells, the apoptotic rate was increasing with the increase of the time and dose of genistein.
Apoptosis ; Cell Cycle ; Cell Line, Tumor ; drug effects ; Cell Proliferation ; Flow Cytometry ; Genistein ; pharmacology ; Humans ; Laryngeal Neoplasms ; pathology

Humans ; Psychological Tests ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; psychology

Objective　To study the ultrastructure of macular pucker (MP) from the patients with rhegmatogenous retinal detachment (RRD) and the mechanism associated with its development．　Methods　Twenty specimens of MP surgically removed by vitrectomy from 13 patients were dissected into two layers in each of them．The ultrastructure of two layers,i,e,near the vitreous and near the retina,was studied with electron microscopy．　Results　Seven sections of the near vitreous ones appeared prodominant collagen deposits and a few of epithelial-like cells,and pigment particles might be present in the cytoplasm．While cells with foot processes were found in 13 membrane sections near the retina and increasing number of various types of cells rich in collagen around were observed including fibroblast-like cells and glial cells．　Conclusion　The findings suggest that the MP after surgery of retinal detachment may possess a characteristic lamination,and posterior hyaloid cortex was involved in the developmetn of MP．The adhesion between posterior hyaloid cortex and macular area might be a key factor for forming MP．

Spinal cord injury is a traumatic disorder resulting in a functional deficit that usually leads to severe and permanent paralysis.Now several strategies including pharmacological and rehabilitation therapies are not effective treatments for spinal cord injury.Recently stem cell transplantion caused extensive concerns from scholars because of its feasibility in theories and definite effectiveness in experiments.Stem cell types used in spinal cord injury therapy include embryonic stem cells,neural stem cells,mesenchymal stem cells and induced pluripotent stem cells.We extensively review the related articles in recent years,discusse and analyze current situations and perspectives of different stem cells transplantion for treating spinal cord injury.

The pathogenesis of diabetic retinopathy is complicated.The vast network of multiple factors including unifying mechanism,inflammatory reaction,neuron degeneration and metabolic memory of glucose,and the four established pathogenic molecular pathways are hotspots of mechanism research for diabetic retinopathy.Nevertheless,these researches may be only one corner of the iceberg of DR mechanism,and we still face enormous challenges in DR mechanism research.Collaboration with multiple disciplines to study the relationship between DR and diabetes and other systemic diseases,search novel therapy targets may increase the result in an unexpected windfall for DR basic research.