Antibody affinity influences the ant igen-antibody reaction in vivo and in vitro. It is important to routinely determine the antibody affinity in order to elucidate the impacts of this antibody characteristics on the immunoassay and the pathogenetic mechanisms of human diseases. In this paper, a simple method (modified Friguet' s) was reported to directly measure the experimental affinity data of monoclonal antibody in ascites by means of ELISA and to accurately determine the corresponding affinity constants of monoclonal antibody by amendment of underestimation of affinity constants due to the intrinsic properties of Friguet' s, and shows convenience and reliability.

Objective To develop a secure network system to monitor the temperatures in baby incubators.Methods By utilizing patented TAV temperature monitoring system,the running status of temperature-controlled equipments was recorded in digital way,and then it was analyzed and managed dynamically;and real time alarms could be given through networks.Results This system is a kind of security devices independent of baby incubators and can monitor temperatures in baby incubators well.Conclusion This system will not affect original functions of baby incubators,instead it can well monitor the temperatures in both imported and domestic incubators,so that it can improve the safety of facilities being used while decrease medical accidents.

The PTC hot air drying device controlled by a single-chip computer has such advantages as rapid heating, automatic isothermal, weak leakage current and being time-proof, which applies to all calibers of pipeline.

Objective:To investigate the effect of telmisartan and fosinopril on the expression of angiotensin-convertion enzyms 2 (ACE2) in cardiomyocyte after myocardial infarction.Methods:Animal model of acute myocardial infarction were established by ligating the left anterior descending coronary artery of rats. Vehicle, telmisartan,fosinopril,or both drugs combined were giv- en to rats by intubation feeding,from 2 weeks before coronary artery ligation to 2 weeks there after. ACE2 protein expression of myocardium were semi-quantified by SP immunohistochemistry. ACE2mRNA were measured by Reverse Transcriptase Poly- merase Chain Reaction (RT-RCR). Results:Compare with sham operated group,ACE2 protein increased significantly in infarc- tion group,but not the ACE2 mRNA. telmisartan and fosinopril therapy caused a significant increase in ACE2 protein compare to infarction group,but only telmisartan caused a significant increase in ACE2 mRNA though all therapied groups showed higher ACE2 mRNA expression. Conclusion:Both telmisartan and fosinopril induced increases in cardiac ACE2 exprssion, whereas the combination of these two drugs do not associated with higher ACE2 expression.

Several researches have suggested that estrogen contributes to the initiation and development of thyroid cancer by binding to estrogen receptors (estrogen receptor α,estrogen receptor β,and G-protein-coupled receptor 30),activating gene or non-genomic pathways and regulating proliferation of thyroid cells.Studies on antiestrogen drugs based on inhibiting thyroid cell proliferation may provide a new target in treating thyroid cancer.

Objective To investigate the effects of aminolevulinic acid-based photodynamic therapy(ALA-PDT) on chicken combs, an animal model for port wine stains (PWS), and to explore the feasibility of PWS treatment with ALA-PDT. Methods A total of 80 leghorns were randomly and equally divided into 10 groups: blank control group receiving no treatment, ALA group treated with ALA alone, four single laser groups irradiated with 630-nm red laser at 75, 100, 150 and 200 J/cm2 respectively, four ALA-PDT groups pretreated with ALA followed by 630-nm red laser radiation at 75, 100, 150 and 200 J/cm2 respectively. An area sized 1 cm × 1 cm were marked at one side of combs in all these leghorns, and served as the experiment area to receive corresponding treatment, with that in the other side as the control area. Tissue specimens were obtained on the 14th and 28th days after treatment followed by the observation of morphological and histological changes, calculation of decrement rate in capillary number, and determination of apoptosis index in vascular endothelial cells (VECs) in chicken combs. Results In all the four ALA-PDT groups, the combs became lighter in color with apoptosis of some VECs as well as a decrease in capillary count and diameter in the dermis of the experiment areas. The decrement rate in capillary number was 33.53% ± 4.89%, 52.02% ± 2.77%, 67.48% ± 5.58%and 88.96% ± 2.47% respectively, and apoptosis index in VECs was 63.44 ± 1.09, 88.50 ± 6.11, 94.32 ± 3.67 and 113.76 ± 10.57 respectively, in the 75-, 100-, 150- and 200-J/cm2 ALA-PDT groups on the 14th day after treatment, and both the decrement rate and apoptosis index in each of these groups were significantly different from those in the blank control group, ALA group, single laser groups receiving red laser radiation at the corresponding dose, and the other ALA-PDT groups (all P < 0.01)separately. The apoptosis depth of VECs, defined as the vertical distance from the basal layer to the deepest level at which VEC apoptosis occurred, was 201.19 ± 0.33 μm, 266.15 ± 1.02 μm, 546.09 ± 2.45 μm and 766.37 ± 1.08 μm respectively in the 75-, 100-, 150- and 200-J/cm2 ALA-PDT groups on the 14th day, with significant differences between these four groups (all P < 0.01). Conclusions ALA-PDT can markedly damage capillaries in the animal model of port wine stains, chicken combs, with the degree and depth of capillary damage associated with red light energy density. The induction of VEC apoptosis may be an action mechanism of ALA-PDT in the treatment of PWS.

Objective To discuss the manifestations, diagnosis and management of acute superior mesenteric artery occlusion (ASMAO). Methods The clinical data of 15 cases of ASMAO admitted in our hospital between 2000 and 2007 were analyzed retrospectively. Results The causes of ASMAO were mesenteric artery embolism in 11 patients and mesenteric artery thrombosis in 4 patients. All cases underwent emergency laparotomy, in which 2 cases underwent removal of the embolus, 11 cases were subjected to resection of necrotic bowel after removal of the embolus, and 2 cases gave up definite surgery. Postoperative heparin anticoagutherapy was used in all patients. The overall mortality rate was 27% (4 cases). Conclusions Acute superior mesenterie artery occlusion should be suspected in a patient with sudden abdominal pain, especially in those who have organic cardiac disease. Early diagnosis and prompt embolectomy are the key to save the patients.

Neurology is a professional science. The content of neurotomia, sub-speciality knowl-edge and auxiliary examination are abundant, and meanwhile it is rather difficult. Clinical graduates after graduation are malnly engaged in clinical work, clinical services, and will play a variety of roles in the course of their clinical practice so it is necessary for them to learn comprehensive knowledge. This article introduced experience of clinical postgraduates tralning of neurology, such as doctor-patient communication skills, basic theoretical study (neurology, neurotomia and sub-speciality knowledge), clinical thinking, and assistant examination, clinical research capacity during these 18month. It will provide the theoretical and practical knowledge in the future work.

Objective To analyze the clinical features and results of array-comparative genomic hybridization (array CGH, aCGH) in a Chinese girl with Phelan-McDermid syndrome. Methods The clinical symptoms of a child with Phelan-Mc-Dermid syndrome were retrospectively analyzed. Routine G-banding was performed to analyze the karyotype, and the aCGH was used to analyze subchromosomal abnormalities. Results The routine karyotype analysis showed a normal female karyotype without abnomalities in chromosome number and structure. aCGH analysis finely mapped the deletion of Chr22q13.2-qter. Phel-an-McDermid syndrome was diagnosed for this case. Conclusions Phelan-McDermid syndrome can be diagnosed by the typi-cal and detailed clinical features in combination with the laboratory examinations of subchromosomal abnormalities. aCGH is one of the most valuable methods to analyze subchromosomal abnormalities and to diagnose Phelan-McDermid syndrome.

Objective To observe the clinical efficacy of electroacupuncture plus umbilical moxibustion in treating gastrointestinal dysfunction after gynecological abdominal operation.Method Totally 112 eligible patients undergone gynecological operation were randomized into group A of 37 cases, group B of 38 cases, and group C of 37 cases. Group A was intervened by electroacupuncture, group B was by umbilical moxibustion, while group C was by electroacupuncture plus umbilical moxibustion. After a treatment course, the symptom scores, restored time of bowel sounds, anal exhaust time, motilin (MTL), gastrin (GAS), and vasoactive intestinal peptide (VIP) contents were observed and compared, and the clinical efficacies were compared between the two groups.Result The symptom scores of the three groups were significantly changed after the intervention (P<0.05). After the treatment, the symptom score of group C was significantly different from that of group A and B (P<0.05). The restored time of bowel sounds and anal exhaust time in group C were significantly different from that of group A and B (P<0.05). The MTL, GAS, and VIP contents of the three groups were significantly changed after the intervention (P<0.05). The MTL, GAS, and VIP contents of group C were markedly different from that of group A and B (P<0.05). The total effective rate was 86.5% in group A, versus 81.6% in group B and 97.3% in group C. The total effective rate of group C was significantly different from that of group A and B (P<0.05).Conclusion Electroacupuncture plus umbilical moxibustion is effective in treating gastrointestinal dysfunction after gynecological abdominal operation.