Type 1 diabetes mellitus(T1DM),also known as insulin dependent diabetes mellitus (IDDM), is classified as an autoimmune disease.Academia currently define it as one of the important autoimmune diseases(IAIDs).The most significant pathophysiological characteristics of T1DM are the pathological humoral and cellular autoimmune response which results in damage to pancreaticβ-cell and absolute reduction insulin.Thus, the mainstream traditional therapy for T1DM has been to chronically replenish insulin or substitutes.Nevertheless, this approach may generate a number of side effects, especially hypoglycemia reaction, hypoglycemic coma and insulin resistance.In recent years, the clinical use of methotrex-ate(MTX) as an immunity inhibitor or regulator aiming at pathogenesis of T1DM has received increasing attention and will become another significant case where an old drug is used for new purposes.The advances in research on MTX in T1DM are discussed in this paper.

OBJECTIVE: To provide a method for the microbial limit test of Anshenning oral liquid and carry out validation of the mothed. METHODS: The validation on the microbial limit test method was conducted in accordance with the counting method of bacteria, mycetes and yeasts and the control bacteria test method stated in the Appendix of China Pharmacopoeia(2010 edition). Culture dilution method was used in bacterium count and total combined molds and yeasts count; while routine method was used in the control bacteria test. RESULTS: The recoveries of test organisms in the control group 3 preparations by routine method were more than 70%. The recoveries of the test group were more than 70%. In the 3 control bacteria tests the negative control bacterias, was not found in the negative control group, while the control bacteria was found in the test group. CONCLUSION: The established method substances can eliminate the antimicrobial in Anshenning oral liquid is accurate, and comprehensive, and can be used for the microbial limit test of Anshenning oral liquid.

The relation between patent applications and published papers in hospitals of Guangdong Province since 2000 on China Intellectual Property Network was analyzed , which showed that the quality and quantity of patents were lower in hospitals of Guangdong Province than in those of Shanghai and Beijing , the quality of patents was re-lated with the medical service level in hospitals, the patent applications were centralized in large class A hospitals of Guangzhou , and inventive patents were those of medical preparations .Suggestions were thus put forward for hos-pitals of Guangdong Province to improve their awareness of patent application, the quality of patents, transformation of patents, cooperation with patent service organizations, research of patent application, and balance of patent dis-tribution in different areas.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Facial Muscles ; physiopathology ; Facial Paralysis ; physiopathology ; therapy ; Female ; Humans ; Male ; Middle Aged

OBJECTIVE To discuss the common problems and their management in the endoscopic nasal dacryocystorhinostomy(DCR) for recurrent dacryocystitis.METHODS A total of 44 patients with recurrent dacryocystitis were performed in our hospital from July,2003 to July,2008.The clinical data of all the cases were studied.All the cases were followed up for over 6 months.RESULTS The common problems met in the operation according to the occurrence frequency were bleeding,difficult for localizing the lacrimal sac,the size and the location of the bony ostium,the size of lacrimal ostium and sinonasal diseases.The cure rate was 93.18%(41/44). CONCLUSION Special instruments,skillful handling and familiar with intranasal anatomy of the lacrimal sac area are the keys to improve the successful rate of endoscopic nasal dacryocystorhinostomy.

Objective In order to study the relationship between the platelet activation sign:P-selectin(GMP 140 ) expression and human IgA nephropathy.Methods Expression of platelet membrane glucoprotein Ⅱb/Ⅲa(GP Ⅱb ,GP Ⅲa ) in blood for IgA nephropathy patients(n=46) was investigated by flow cytometry methods.Results In IgA nephropathy the up-regulated GMP 140 expression in blood was significantly higher than that in normal humans.Conclusions GMP 140 might play an important role in pathogenesis of IgA nephropahty,and the level of GMP 140 in blood might predict renal outcome in IgA nephropathy.

OBJECTIVE To evaluate the choice of microsurgical treatment modalities and it's clinical effect on middle ear cholesteatoma and chronic suppurative otitis media. METHODS 110 cases with middle ear cholesteatoma and chronic suppurative otitis media were analysed which performed canal wall up mastoidectomy and canal wall down mastoidotympanectomy or at the same time tympanoplasty depending on lesion extent and the recovery time, complications, recurrence and postoperative hearing improvement were followed-up. RESULTS 110 cases were reviewed including 66 cases of middle ear cholesteatoma, 44 cases of chronic suppurative otitis media. Among these 51 cases undergone canal wall up mastoidotympanectomy accounting for 46.36%, 46 case undergone tympanoplasty, accounting for 41.81%. 59 cases undergone canal wall down mastoidectomy, accounting for 53.64%. In the canal wall up mastoidectomy group, 37 ears got hearing improvement >25 dB(33.64%), >15 dB 14 ears(12.72%), while in the canal wall down mastoidectomygroup, 5 ears got hearing improvement>15 dB(4.55%), 4 ears hearing loss(3.64%). Statistically significant difference exist in preoperative and postoperative speech frequency of average hearing threshold. We have found 4 cases of recurrence with cholesteatoma after 1 year follow-up. CONCLUSION According to different lesions of middle ear and mastoid, different operative methods can be used to obtain the corresponding clinical curative effect.

Adenoma, Pleomorphic ; surgery ; Humans ; Trachea ; Tracheal Neoplasms ; surgery ; Tracheotomy

Adenocarcinoma, Clear Cell ; Humans ; Laryngeal Neoplasms ; Male ; Middle Aged

Objective To determine the evaluating prognostic value of plasma Nt-proBNP in patients with congestive heart failure.Methods 107 patients with congestive heart failure were included in this study,followed the degree of rehospitalization and mortality rate of the cured patients after left hospital.The plasma concentrations of Nt-proBNP were measured by enzymelinked immunozorbent assay(ELISA).Results Concentrations of Nt-proB-NP of Ⅱ,Ⅲ,Ⅳ classincation were(1129.8±383.2),(5849.7±567.6),(9506.3±492.4)ng/L in NYHA functional classification respectively(P＜0.01);the plasma level of Nt-proBNP had significant positive correlation with NYHA functional classification(r=0.759,P＜0.01).The degree of rehospitalization were(0.85±0.96),(1.98±0.86),(2.58±0.91)respectively(P＜0.01);mortality rate were 8.3%,12.5%,45.2%respectively(P＜0.01).Conclusion Plasma Nt-proBNP level may be the more important clinic value in evaluating prognosis in patients with congestive heart failure.