Objective To generate brevican knockout mutant mice. Methods The targeting construct to inactivate the brevican gene was made by using the ET cloning technology. The construct was linearized and electroporated into embryonic stem (ES) cells. Homologous recombinants were identifed by PCR after selecting the stable transfectants. The positive clones were then injected into C57BL/6 blastocysts, and the injected blastocysts were transferred into pseudopregnant foster mothers. Heterozygote and homozygote were multiplied by procreation mosaic. Progeny gene type was assayed by PCR. Results A neomycin resistance expression cassette under the control of the phosphoglycerate kinase (PGK) promoter was flanked by a 2.4-kb 5-arm fragment and a4.8-kb 3-arm fragment, thus introducing the NEO gene into exon 3 and deleting the encoding region from exon 3 to exon 8 after homologous recombination. Fourteen clones of gene-targeted ES cells were identified and three male chimeras with a higher than 50% chimeric ratio were produced. Six brevican-null mice were generated after outbred and inbred. Conclusion Genetically modified ES cells have been successfully generated by homologous recombination, and brevican-deficient mouse strain has been successfully generated.

Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Female ; Headache ; therapy ; Humans ; Male ; Middle Aged ; Young Adult

Objective To study the diagnostic value of MRCP in cholangiocarcinoma.Methods MRCP in 39 patients with cholangiocarcinoma comfirmed by surgery and pathology were performed on a GE 1.5T superconductive MR imaging unit before operations.Results MRCP imagings with diagnostic value were obtained in 39 cases,MRCP accurately showed the obstructive positions confirmed by surgery and pathology in all cases (100%).The qualitative rate of cholangiocarcinoma was 97%(38/39).Conclusion MRCP can not only accurately show the position of cholangiocarcinoma but also make accuracy diagnosis.

The relation between patent applications and published papers in hospitals of Guangdong Province since 2000 on China Intellectual Property Network was analyzed , which showed that the quality and quantity of patents were lower in hospitals of Guangdong Province than in those of Shanghai and Beijing , the quality of patents was re-lated with the medical service level in hospitals, the patent applications were centralized in large class A hospitals of Guangzhou , and inventive patents were those of medical preparations .Suggestions were thus put forward for hos-pitals of Guangdong Province to improve their awareness of patent application, the quality of patents, transformation of patents, cooperation with patent service organizations, research of patent application, and balance of patent dis-tribution in different areas.

Background and purpose:Patients with colorectal cancer are often accompanied by the increase of plasma ifbrinogen concentration. This study aimed to investigate the distribution characteristics of beta-ifbrinogen gene-448G/A, -148C/T, -1420G/A and -854G/A polymorphism and plasma ifbrinogen (Fg) concentration in patients with colorectal cancer. Furthermore, we analyzed their effects on the occurrence and development of cancer.Methods:The level of plasma Fg was quantiifed by using Clauss clotting method.FGBβ gene polymorphisms were identiifed by re-al-time lfuorescence quantitative PCR (RTFQ-PCR) in 194 colorectal cancer patients and 74 healthy controls.Results:The plasma Fg levels in tumor metastasis group and non-metastasis group were signiifcantly higher than that in control group, respectively (P<0.05). Compared with control group, the frequencies of -148T allele and mutation genotype were notably higher in disease group (P<0.05). In all the groups, the plasma Fg levels of those with -148T allele were higher than those without -148T allele (P<0.05). In stageⅣ patients, there was no difference in PFS between -148T wild gen-otype group and mutation genotype group (P>0.05).Conclusion:Plasma Fg concentration in patients with colorectal cancer was signiifcantly raised, which suggests that Fg may play a role in the occurrence and development of colorectal cancer. The beta-ifbrinogen gene -148C/T polymorphism is one of the reasons that cause plasma Fg elevation, but has no correlation with prognosis of patients with stageⅣ colorectal cancer.

Objective Trying to predict the degree of GVHD after partly matched hematopoietic stem cell transplantation. Methods Analysis of the relationship between three-dimensional structure differences of donor-patient unmatched HLA and the GVHD levels after hematopoietic stem cell transplantation. Results GVHD levels were related to donor-patient unmatched HLA structure differences. The HLA structure differences forⅠ - Ⅱ degree GVHD were much smaller than that for Ⅲ - Ⅳ degree GVHD. Conclusion Prediction of GVHD by HLA structure differences is simple, rapid, specific and could help select proper conditioning regimens before transplantation and the proper immune suppressive agents after transplantation.

Objective To investigate the effect of cardiac resynchronization therapy on improving heart function and reversing left ventricular remodeling in chronic heart failure patients. Methods Eleven patients with NYHA class Ⅲ to Ⅳ heart failure, ejection fraction ≤35% and QRS duration ≥130 ms were implanted synchronous biventricular pacemaker. Serial assessment was performed before and up to 1-36 months after pacing. Results There were significant improvement of LVEF、 LVFS; and decrease in MR、 LVED and QRS duration. The NYHA class of heart function was improved from Ⅲ-Ⅳ to Ⅰ-Ⅱ. Six-minute hall walk distances were also improved. Conclusion For patients with NYHA class Ⅲ to Ⅳ heart failure and ventricular conduction delay, cardiac resynchronization therapy could reverse left ventricular remodeling and improve heart function.

Objective To determine the content of gallic acid and evaluate the quality of Galla chinensis of different habitats in Guizhou Province. Methods HPLC method was used to determine the content of Gallic acid on Alltima C18 column (4.6 mm?250 mm, 5 ?m) with the mobile phase of methanol-water-phosphoric acid (15∶85∶0.085). The flow rate was 1.0 mL/min and the detective wave length was 270 nm. Results The contents of Gallic acid in Galla chinensis of 10 different habitats in Guizhou Province were 51.43%～ 60.25%. Conclusion The quality of Galla Chinensis in different habitats in Guizhou Province are up to the Chinese Pharmacopoeia, and the individual varibility is significant.

N400 as one of endogenic components in event-related potentials,which reflected the cognitive process of language in brain,has been more and more widely concerned.This article reviewed the discovery,the characteristics,the origins and the elicited methods of N400,summarized the investigations and point at issue on the cognitive process of language over the past several years,and explored the application foreground of N400 in the field of language cognition.

Objective:TO provide theoretic evidence for Chinese rehabilitation training to study the characteristics of right hemisphere in recognizing Chinese and association between left and right cerebral hemispheres.Methods:102 cases up to the qualification,including 41 patients with right-hemisphere-lesion(RHL)、 31 patients with left-hemisphere-lesion(LHL) and 30 healthy individuals as control,were collected and tested with The Standardized Aphasia Battery in Chinese(ABC)、Writing-scopy of Chinese Character and Visual Spatial Structure-scopy.Results:14 aphasic patients,including 1 RHL and 13 LHLs,were found 11 of 72 patients had apparent reading disorder,including 7 LHLs with language reading disorder and 4 RHLs with neglect reading disorder;and 29 of 72 patients had apparent writing disorder,including 13 LHLs with language writing disorder and 16 RHLs among whom 9 cases had visual space writing disorder and 7 cases had language writing disorder;RHLs showed lighter listenning comprehension disorder than that of LHLs in the test.Conclusion:Right hemisphere is mainly involved in Chinese written language recognition and processing,and one of the most important reasons of reading and writing disorder is left side neglect、visual consciousness and spatial analysis dysfunction due to RHL.