BNP (B-type natriuretic peptide) and NT-proBNP (N-terminal pro-B-type natriuretic peptide) are peptide hormones secreted by the heart.Recently, many researches have described that BNP and NT-proBNP were useful markers for the diagnosis of heart failure.We should develop these assays correctly on the basis of full understanding of biological characteristics and guide clinical use for them in medical laboratories.

Background:Inflammatory bowel disease(IBD)is a chronic,non-specific,relapsing inflammatory bowel disorder. Prediction of prognosis and relapse is of great importance for disease control. Aims:To analyze the characteristics and related factors for relapse in IBD patients. Methods:Four hundred and seventy-nine hospitalized patients diagnosed as ulcerative colitis(UC,n = 301)or Crohn’s disease( CD,n = 178)from Jan. 2004 to Jun. 2014 were enrolled and divided into relapse group and non-relapse group. The clinical characteristics were collected and univariate and multivariate analysis were performed to identify the influencing factors for relapse. Results:The relapse rates for UC and CD were 52. 49% and 77. 53% ,respectively. In stepwise Logistic regression analysis,younger onset age( < 40 years old,OR =0. 825,95% CI:0. 777-0. 876,P = 0. 000),longer disease duration(OR = 1. 186,95% CI:1. 089-1. 291,P = 0. 000) and extensive colitis(OR = 16. 993,95% CI:2. 670-108. 170,P = 0. 003)were independent risk factors for relapse of UC,while younger onset age( < 40 years old,OR = 0. 944,95% CI:0. 915-0. 974,P = 0. 000)and poor medication adherence(OR = 31. 074,95% CI:10. 456-92. 343,P = 0. 000)were independent risk factors for relapse of CD. Conclusions:Patients at high risk for relapse of IBD can be identified by referring to the related risk factors. Early identification and intervention,persistence for maintenance therapy and improving medication adherence by health education may reduce the clinical relapse of IBD.

Objective To explore the anti-inflammatory therapeutic role of erythromycin in chronic obstructive pulmonary disease(COPD) by investigating the effect of erythromycin on the level of nuclear factor kappa B(NF-?B) and intercellular adhesion molecule-1(ICAM-1) in airway epithelium of smoking rats.Methods Wistar rats(n=30)with weighed(150?10) g each,20 were randomly sampled to be exposed to cigarettes smoking for four weeks then 10 of them were exposed wntinuously to cigaretts smoking for eight weeks as smoking,group,other loof them were intragastrically perfused by erythromycih before smoking as evythromycin group,the rest of total 30 rats as control group Then assessed expiratory airway resistance and lung compliance and observed the expression level of NF-?B and ICAM-1 in airway epithelium by using immunohistochemical method.Results(1) The expiratory airway resistance was increased and the lung compliance was degraded significantly in smoking group(5.67?0.32,4.39?0.40)(P

BACKGROUND: Any surface treatments should Improve the bond strength of yttria-stabilized tetragonal zlrconia polycrystal ceramics without damaging the original strength. Currently, there is no Information about the influence of glaze technology on the bond strength of yttria-stabilized tetragonal zlrconia polycrystal ceramics, and its Influence on the material strength Is unclear. OBJECTIVE: To evaluate the effect of glaze technology on the mechanical behavior of yttria-stabilized tetragonal zlrconia polycrystal ceramics and on the bond strength with resin cements. METHODS: Yttria-stabilized tetragonal zlrconia polycrystal ceramics specimens were prepared and randomly divided into four groups: group A: the surface was not treated; group B: sandblasting with 110 urn Al203 particles; group C: glaze + hydrofluoric acid etching; group D: glaze + hydrofluoric acid etching + sllanlzation. The surface morphology, surface roughness, phase transformation, elemental composition, shear bond strength and flexural strength of zlrconia specimens after surface treatments were tested. RESULTS AND CONCLUSION: (1) The surface roughness of treatment groups increased obviously, whose descending order was group C (0.62±0.01) um > group D (0.55±0.02) um > group B (0.11±0.02) um > group A (0.05±0.01) urn (p < 0.05). (2) The surface of specimens in the group B contained 2.2% monoclinic zirconia, while the content of other groups was zero. (3) In addition to elements of Zr and O, there was 6.49% Al element in the group B specimens, and 18.67% and 25.78% Si element In the groups C and D specimens, respectively. (4) The shear bond strength in the groups A, B, C and D was (3.11±0.40), (4.23±0.45), (6.62±0.60) and (10.46±0.83) MPa, respectively (P < 0.05). (5) The three-point flexural strength in the groups A, B, C and D was (961.07±75.53), (1 234.73±114.09), (1 024.28±120.51), and (1 036.09±80.10) MPa, respectively, among which the multiple comparison of groups A, C and D showed no significant difference (P > 0.05). (6) The effect of glaze technology on improving the flexural strength of yttria-stabilized tetragonal zirconia polycrystal ceramics is not as obvious as that of sandblasting, but the bond strength between tetragonal zirconia polycrystal ceramics and resin cements can be significantly Improved with hydrofluoric acid etching and silanization after glaze.

Objective: To develop a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method with cation exchange-based solid phase extraction (SPE) for determination of tetrodotoxin (TTX) in salted pufferfish. Methods: Evenly crushed salted pufferfish samples were subjected to ultrasound-assisted extraction with 0.5% acetic acid/50% methanol/water. The extract was cleaned with cation exchange-based SPE cartridge and eluted with 0.3% hydrochloric acid and 50% acetonitrile/water. The eluent was neutralized with ammonia and separated with a Waters XBridgeTM BEH Amide column (150 mm×3.0 mm, 1.7 μm), and determined using LC-MS/MS in a multiple reaction monitoring mode. Results: The matrix effects of TTX were 85.7%-92.4%, and the matrix suppression effect was under effective control following clean-up procedures using the optimized SPE method. The TTX showed a good linear relationship at the range of 2.0 to 4 000 μg/kg, with a correlation coefficient (r2) of 0.999 2. The limits of detection and quantitation for TTX in sample matrix were 1.0 μg/kg and 2.0 μg/kg, respectively. The mean spiked-recovery rates were 81.2% to 96.5% at spiked amounts of 2.0, 200 μg/kg and 2 200 μg/kg, with relative standard deviations (RSDs) of 4.3% to 7.5%. The intraday accuracy and precision of TTX were 84.4% to 95.6% and 4.9% to 5.8% in quality control samples, and the interday accuracy and precision of TTX were 86.1% to 94.9% and 5.5% to 8.5% in quality control samples. The detection of TTX was 60.5% in 38 market-sold salted pufferfish products using the established LC-MS/MS method. Conclusion The established LC-MS/MS method is effective for accurate quantitative determination of TTX in salted pufferfish.

Objective To study the status quo of the caring character of baccalaureate nursing students and its related factors.Methods A total of baccalaureate nursing students were recruited from two and three levels of baccalaureate in Nantong district by convenience sampling method and cluster sampling method.The students were investigated with a nurse caring character questionnaire which was developed in the previous study and then an analysis was conducted.Results The caring characters of the nursing baccalaureate students were at the medium level.The caring characters of baccalaureate nursing students were affected by various factors.The linear regression revealed that choosing nursing willingly or not,relationship with familyand sex,among other things had the greatest influence on their caring characters.Conclusions Therefore,importance should be attached to the caring character of baccalaureate students and corresponding guidance and education directed at the various grades should be offered so as to increase their caring characters.

74 years were associated with increased cardiac events too(HR = 5.40,95%CI:2.16~11.52,P=0.0002).NYHA classification, LVEF, cTnT?cTnI and CK-MB were not independently predictive of cardiac events.Conclusions Measurement of NT-proBNP in patients with CHF can help to identify patients at higher risk for cardiac events .

Objective To investigate the effect of self- management programme in home care of oncohematologic patients with peripherally inserted central catheters(PICC). Methods Seventy oncohematologic patients with PICC were divided into the control group and the intervention group with 35 patients in each group by random digital table method. The control group received the routine care. The intervention group received the self- management programme. Self- care ability, anxiety, catheter- related complications and maintenance cost were observed before and on the 10th day and 30th day after intervention. Results On the 10th day and 30th day after intervention, the total score and scores of each dimension of self- care ability in the intervention group were significantly higher than those in the control group, P<0.05 or 0.01, the differences were significant. The score of anxiety in the intervention group was significantly lower than that in the control group [for the patients on the 10th day, 47.64±4.40 vs. 51.14±6.28,on the 30th day,44.36±3.71 vs. 48.64± 5.54, t=3.40, 5.18, P<0.01; for the family members of patients on the 10th day, 47.36±2.12 vs. 50.57±3.99, on the 30th day,43.57±2.04 vs. 47.57±2.79, t=4.35, 7.42, P<0.01. The incidences of catheter- related complications were not significantly different between two groups, P>0.05. Conclusions Self- management programme can effectively improve the self-care ability, relieve the anxiety and prevent the catheter- related complications in home care of oncohematologic patients with PICC.

Objective To investigate the influence of renal function on serum NT-proBNP in the diagnosis of chronic heart failure by observing the relationship between eGFR and NT-proBNP in serum and comparing cutoff values of NT-proBNP in different eGFR levels.Methods 297 elderly participants were enrolled in the study, including 106 inpatients with heart failure and 191 healthy controls in the corresponding period.The concentration of NT-proBNP was determined by an automated electrochemiluminescence immunoassay on Roche Elecsys 2010.Estimated glomerular filtration rate (eGFR) was calculated using the Levey-modified Modification of Diet in Renal Disease formula. The diagnosis of clinical physician was considered to be the golden standard for heart failure.Results Serum NT-proBNP levels were significantly higher in subjects with renal dysfunction compared with those with normal renal function for heart failure patients [829.1 (202.4 ~ 3725.5) ng/L vs. 227.2 (111.1 ~ 964.2) ng/L, P