Objective of electroacupuncture(EA) of Alzheimer′s disease(AD) rat learning and memory ability and plasma SOD , MDA content in brain P53 ,P21 expression .Methods The normal 36 SD rats ,were randomly divided into sham operation group , model group and EA group .EA group acupuncture “Baihui” ,“Taixi” ,“Zusanli” treatment ,through the Morris water maze test in rats behavioral changes ,spectrophotometric observation of SOD and MDA in rat plasma levels ;observation of rat brain P53 ,P21 ex-pression by immunofluorescence .Results Compared With the model group ,group EA escape latency ,longer in the third quadrant of the activities of the time ,the number of crossing platform position increased ,decreased the elevated plasma SOD level ,MDA lev-el ,reduce the expression of brain P53 ,P21 ,and the difference was statistically significant (P<0 .01) .Conclusion electro-acupunc-ture treatment can improve the Aβ1-42-induced AD rat spatial learning and memory ability ,may be lowered P53 ,P21 protein ex-pression increase SOD activity and MDA .

Cytochrome P450(CYP450)is the most important family of enzymes in microsomal mixedfunction oxidase,widely distributed in vivo.CYP450 is involved in the metabolism of many exogenous compounds,the generation of endogenous substances,in particularly,affecting the occurrence and development of the tumors and their drug treatment,in the fields of medical and pharmaceutical research,cytochrome P450 has been very noticeable.

Objective To study the correlation between diabetes and the new discovered angiopietin-related growth factor (AGF)that participates in energy metabolism.MethodsA total of 24 male C.57BL/6 mice were divided into three groups:controlled group; fed with high-fat diet( HFD); fed with high-fat diet for 10 weeks and then treated with rosiglitazone ( HFD + RSG) ; The relative level of AGF mRNA expression in livers was measured by determining a ratio of PCR products of AGF to that of β-actin gene.Oral glucose tolerance test,and insulin resistance index (HOMA-IR) were used to measure blood glucose level and insulin sensitivity.Results Compared with the controls,the expression of AGF mRNA in liver decreased significantly in HFD group( P ＜0.05),and increased significantly in HFD + RSG group( P＜0.01 ).A negative correlation was found between AGF mRNA expression in mice livers and HOMA score ( r =-0.516,P ＜ 0.05 ).Conclusions AGF as a new hepatocyte derived circulating factor counteracts obesity and is related to insulin resistance.

Liver metastasis is the leading cause of death from colorectal cancer.Surgical treatment is so far the best choice for the treatment of colorectal liver metastases (CRLM).However,how to suitably select an individualized chemotherapy in perioperative period and surgical time and tool is still under controversy.Therefore,the key factors of improving the prognosis of patients with CRLM include overall evaluation of the patients and their preoperative examination outcomes,dividing patients into different groups based on different therapeutic goals,understanding different therapeutic goals of each group,selecting suitable chemotherapy and targeted therapy,ensuring surgical time (synchronous or stepwise resection) and tools (minimally invasive or open surgery) through multidisciplinary team model.

Objective To explore the effect of brief group psychological counseling in promoting students psychological health of high grade in primary school. Methods Two classes were randomly selected from a primary school in a community of Pudong New Area in Shanghai from October 2014 to November 2014, with stratified cluster sampling method in 4,5 grade ( the boy 30, the girl 24) as the research object, the other 2 classes (4,5 grade 1 classes;the boy 25,the girl 31) of students as control group, in the intervention group students brief group counseling was carried out, while the control group received the routine psychological health education course. Using the mental health diagnosis test (MHT) as a psychological assessment tool, MHT scores of two groups of students and factors were analyszed by the test analysis. Results Before intervention, the intervention group and the control group in the mental health diagnosis test (MHT) had no significant difference in each factor (P>0.05). After the intervention, significant were found differences between the intervention group and the control group in MHT learning anxiety, anxiety to people, self accusation tendency, allergic tendency, body symptom, terror tendency and the total score(6.46±3.01, 8.59±3.40; 3.04±1.91, 4.45±2.43; 3.56± 2.13, 5.30 ± 2.10; 4.37 ± 1.93, 5.84 ± 2.63; 3.76 ± 2.82, 5.46 ± 3.38; 2.15 ± 2.34, 3.91 ± 3.00; 32.44 ± 13.07, 44.52±17.59)(t=-3.47、-3.38、-4.30、-3.35、-2.87、-3.40、-4.10 ,P<0.05), and the average scores in the intervention group of each factor were lower than those of control group. Conclusion Brief group psychological counseling for higher-grade students of elementary school learning anxiety group, had better interventional effect on anxiety, self-blame tendency, the overall tendency of anxiety and other fields. It will have a positive impact on the allergic tendency, body symptom, terror tendency and other fields; it needs further evaluation in large sample for long-term effect.

OBJECTIVE: To explore the association of XRCC3 gene polymorphisms and haplotypes with laryngeal. METHOD: We selected 4 tag SNPs (rs12432907, rs861536, rs861537, rs861531, rs861531) for the present study. 310 laryngeal patients and 310 healthy control subject were genotyping. The distribution of genotypes and haplotypes in these two group was compared. RESULT: The distributions of rs12432907 was significantly different between these two groups. The CCAG haplotype frequency was higher in laryngeal group than that in control group. But TCAG and TTAG haplotype frequency was were lower in the laryngeal patient than that those in the control subject. CONCLUSION XRCC3 gene polymorphism was associated with the risk of laryngeal patients.
Case-Control Studies ; DNA-Binding Proteins ; genetics ; Genotype ; Haplotypes ; Humans ; Laryngeal Neoplasms ; genetics ; Polymorphism, Single Nucleotide

Objective To study the effects of miR-130b on the neuronal migration in the developing embryonic mice cortex.Methods Pregnancy E15.5 mice were selected and plasmid of miR-130b was injected into the lateral ventricle of the embryonic brain.Two days after eletroporation,murine embryos were collected and cut into frozon coronal slices,then surveyed the neuronal migration under the fluorescence microscope.Results The neuronal migration rates were higher in miR-130b overexpression embryonic cortex,of which 75.1％ eletroporated neurons migrated into MZ,compared with the 22.1％ in control group.On the contrary,only 7.9％ eletroporated neurons stayed in the VZ/ SVZ in the experimental group,compared with the 69.3％ in control group.Conclusions miR-130b accelerates the neuronal migration in the developing embryonic mice cortex,its regulational role is worth studying.

ObjectiveTo investigate the clinical and imaging features of nonaneurysmal perimesencephalic subarachnoid hemorrhage (NAPSAH).MethodsThe patients with NAPSAH were retrospectively enrolled, and their clinical and imaging features were collected and analyzed.Results In 489 patients with subarachnoid hemorrhage treated over the same period, a total of 23 (4.7%) with NAPSAH were diagnosed with cranial CT and CT angiography.Subarachnoid blood centered in the prepontine cistern in 4 patients (17.39%), interpeduncular cistern in 8 (34.78%), ring pool in 7 (30.43%), and quadrigeminal cistern in 4 (17.39%).Two patients were transferred to other hospital for treatment, and the remaining 21 patients had a good outcome without complications and neurological deficits.Among them, the modified Rankin scale scores in 17 patient were 0, and 4 were 1.Their cardinal symptoms were forgetfulness, irritability, anxiety, and other subjective symptoms.They were improved after receiving psychological counseling and anti-anxiety treatment.ConclusionsThe incidence of NAPSAH is low.The clinical symptoms in patients with NAPSAH are mild, the outcomes are better without obvious complications.

Objective To explore the mechanism ofTiaozhong Huatan Anshen Mixture (THAM). Methods A total of 200 healthy Kunming mice were randomly and equally divided into first, second, third and fourth major groups. Each major group was further equally divided into blank group, control group and high, medium, low dose THAM groups. Each group was given an equal volume of NS, estazolam, or high, medium, or low dose THAM by gavage for 7 d. For the first major group, the changes in number of spontaneous activities after administration were evaluated. For the second major group, 60 min after administration, each group was given pentobarbital sodium (30 mg/kg body weight, minimum dose for all mice sleeping) by intraperitoneal injection, and the sleep time was recorded. For the third major group, 60 min after administration, each group was given pentobarbital sodium (15 mg/kg body weight, maximum dose for 90%-100% of all mice having righting reflex) by intraperitoneal injection. The number of mice falling asleep was recorded, and the sleep rate was calculated. For the fourth major group, mice were sacrificed after continuous administration 15 d. They were quickly removed brain tissue;homogenates were taken out in batches;5-HT and 5-HIAA levels in brain tissues were determined according to fluorescence spectrophotometric.Results Compared with the blank group, all treated groups had a reduced number of spontaneous activities, a shortened sleep latency, a prolonged sleep time, and an increased sleep rate after administration (P<0.01,P<0.05), with the most significant changes seen in the control group and high-dose THAM group (P<0.05), with out statistical significance between the two groups (P>0.05). Compared with the blank group, brain 5-HT and 5-HIAA levels in all treated groups increased in mice (P<0.01,P<0.05), with the most significant changes seen in the high-dose THAM group (P<0.05).Conclusion THAM has a significant sedative-hypnotic effect. The mechanism may be related to increasing brain 5-HT synthesis and metabolism.

Microinjoction of thy-rotropin-releasing hormone (TRH) into par-avemricular nucleus (PVN ) obiviously increased gastric motility: frequency of gastric motility change from 3~1 times ? min-1 to 6 ~ 8 times ? min-1. intergastric pressure (IGP) rose from 0. 49 ? 0. 01kPa to 1. 9 1 ? 0. 5kPa. (P