1.Research progress on small cell lung cancer with syndrome of inappropriate secretion of antidiuretic hormone
Chinese Journal of Clinical Oncology 2017;44(5):233-237
Many malignant tumors, most commonly small cell lung cancer, merge with syndrome of inappropriate secretion of antidi-uretic hormone (SIADH). SIADH is caused by the neuroendocrine function of tumor cells or is a side effect of chemotherapeutics. As a chemotherapeutic side effect, SIADH presents as hyponatremia. Therapy for SIADH includes both treatment for primary diseases and hyponatremia. Given that SIADH-induced hyponatremia aggravates the prognosis and shortens the survival time of patients with malig-nant tumors, SIADH is an independent risk factor for prognosis. Therefore, the early diagnosis, monitoring, and treatment of SIADH will improve the prognosis of patients with malignant tumors.
2.p38 MAPK signaling transduction pathway and cerebral ischemia
International Journal of Cerebrovascular Diseases 2011;19(11):853-856
p38 mitogen-activated protein kinase (MAPK) is an important intracellular signaling transduction pathway.Its major role is involved in inflammatory regulation and apoptosis.p38 MAPK is activated during the cerebral ischemia.Its expression level and the upstream and downstream protein phosphorylation levels will change.Ischemic preconditioning may mediate the survival or death of neuronal cells after cerebral ischemia through signaling transduction pathway.
3.Microbial contamination in dental unit waterlines A comparison among different specialty departments in a stomatological hospital
Pingping XU ; Xiaochun CHEN ; Xiaoqing SHEN
Chinese Journal of Tissue Engineering Research 2009;13(51):10189-10192
BACKGROUND: The presence of various microorganisms in dental unit waterlines has been revealed by numerous reports previously. However, impact of different dental procedures on microbial contamination in dental unit waterlines (DUWLs) remains unclearly.OBJECTIVE: To assess the level of microbial contamination in water effluent from the high-speed handpiece line of dental chair units (DCUs) among different specialty departments in a provincial stomatological hospital. Thus, to propose individual infection control measures for different specialty departments according to their properties of microbial contamination.DESIGN, TIME AND SETTING: A cross-sectional investigation was designed and conducted in Guangdong Provincial Stomatological Hospital and Laboratory of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, between May 2007 and March 2008.MATERIALS: The materials used in this investigation including nutrient agar, blood plates, biochemical testing kit, agglutination testing kit and Gram staining agents. These materials were purchased from Guangdong Huankai Microbial Science and Technology Co., Ltd.METHODS: Water sampling was conducted at 80 DCUs of different specialty departments in Guangdong Provincial Stomatological Hospital. A total of 60 mL, with 6 mL for once sampling of water effluent from the high-speed handpiece line of each DCU were collected aseptically after the finish of the daily clinical work on every second Friday between May and October in 2007. Standard isolation and identification technique of bacteria was adopted.MAIN OUTCOME MEASURES: Concentration of bacteria and prevalence of 3 species of bacteria in water effluent from the high-speed handpiece line of DCUs among different specialty departments were evaluated.RESULTS: Concentration of bacteria in water effluent from the high-speed handpiece line of DCUs averaged 5.67×10~2 cfu/mL, ranged from 5.15×10 to 1.59×10~3 cfu/mL. The microbial concentration of water samples from Department of Periodontics was significantly higher than that from other departments, while the microbial concentration of water samples from Department of Oral Surgery was the lowest (P = 0.000).CONCLUSION: Contamination in water effluent from the high-speed handpiece line of DCUs varied from different specialty departments in the provincial stomatological hospital. It is suggested that DUWLs flushing should be performed routinely, especially for the department without frequent use of the high-speed handpiece in the daily work, and extra infection control measures should be adopted for the department with severe clinical operative contamination.
4.Microbial contamination in dental unit waterlines with different flushing volume
Pingping XU ; Xiaochun CHEN ; Xiaoqing SHEN
Chinese Journal of Tissue Engineering Research 2009;13(52):10397-10400
BACKGROUND:Daily flushing of dental unit waterlines (DUWLs) is believed to be the least expensive and simplest method for reducing the level of microbial contamination in DUWLs.Authorities' guidelines suggest that high-speed handpieces should be run to discharge water for 30 seconds after use on each patient.However,the guideline is inaccurate for clinicians according to the time-dependent flushing since the maximum flow rate of flushing water varied from dental chair units (DCUs).OBJECTIVE:To assess the level of microbial contamination in water effluent from the air-water syringe and the high-speed handpiece line of DCUs and prevalence of three species of bacteria detected in DUWLs at each flushing volume.Thus,practical water flushing measures could be proposed according to the effect of volume-dependent dental unit waterline flushing on the microbial contamination in DUWLs.DESIGN,TIME AND SETTING:A cross-sectional investigation was designed and conducted in Guangdong Provincial Stomatological Hospital and Laboratory of Microbiology,School of Public Health and Tropical Medicine,Southern Medical University between May 2007 and March 2008.MATERIALS:The materials used in this investigation including nutrient agar,blood plates,biochemical testing kit,agglutination testing kit and Gram staining agents.These materials were supported by Guangdong Huankai Microbiological Science and Technology Ltd.METHODS:Water sampling was conducted at 80 DCUs in a stomatologicel hospital.A total of 6 mL,2 mL for once sampling,of continuous water flushing from the air-water syringe and the high-speed handpiece line of each DCU respectively were collected aseptically after the finish of the daily clinical work on every second Friday,between May 2007 and October 2007.Standard isolation and identification technique of bacteria was adopted.MAIN OUTCOME MEASURES:Concentration of bacteria and prevalence of three species of bacteria in water effluent from the air-water syringe and the high-speed handpiece line of DCUs at each flushing volume were evaluated.RESULTS:The median concentration of bacteria in water effluent from either the air-water syringe or the high-speed handpiece line of DCUs was 5.67×10~2 cfu/mL,and there were no statistical differences among microbial concentrations of first three 2-mL flushing water samples (P>0.05).Prevalence of Staphylococcus spp.from the third 2-mL flushing water sample demonstrated an obviously higher level than that of Staphylococcus spp.from the first two 2-mL (P < 0.05),while prevalence of Streptococcus spp.and Actinomycete spp.kept at a relative stable level (P > 0.05) at each flushing volume.CONCLUSION:Volume-dependent water flushing procedure stays a more practical measure for reducing microbial contamination in DUWLs rather than time-dependent flushing,but the volume of flushing water needs to be further evaluated.
5.The roles of heat shock protein 27 in cerebral ischemic tolerance
Baoxing WU ; Pingping ZHU ; En XU
International Journal of Cerebrovascular Diseases 2011;19(12):948-952
Heat shock protein 27 is one of the members of the small heat shock protein family.Its molecular chaperone function and the interaction with the cell death pathway mediate cell survival under the stress state.The expression of heat shock protein 27 in ischemic lesions and remote areas and its changes in phosphorylation levels during cerebral ischemia is associated with neuronal tolerance to ischemia.However,the neuroprotective mechanism of heat shock protein 27 in cerebral ischemic tolerance still remains unclear.This article reviews the roles of heat shock protein 27 in cerebral ischemic tolerance and its potential mechanism.
6.Clinical value of high frequency two-dimensional and color Doppler ultrasonography for the diagnosis of lower limb deep venous thrombosis
Tingting REN ; Houmei XU ; Pingping REN
Chinese Journal of Postgraduates of Medicine 2012;35(14):19-21
ObjectiveTo explore the application value of high freuency two-dimensional and color Doppler ultrasonography in the detection of lower limb deep venous thrombosis.MethodsEighty-nine patients with suspicious lower limb deep venous thrombosis were enrolled and received high frequency two-dimensional and color Doppler ultrasonography examination.Vessel diameter,thrombus position,size and echo and blood flow in vessel were observed.ResultsIn 89 patients,42 cases (47.2%) had femoral-iliac vein thrombus;27 cases(30.3% ) had vein thrombus in intramuscular of cnemis; 13 cases (14.6%) had deep venous thrombosis of the whole lower limbs; 5 cases (5.6%) had intramuscular haematoma;2 cases (2.2%)had subcutaneous soft tissue edema of cnemis.Eighty-four cases were detected having vein thrombus,2 cases (2.4%) were in both lower limbs; 57 cases (67.9%) were in single left lower limb and 25 cases(29.8%)were in single right lower limb.The ratio between thrombus in single left lower limb and in single right lower limb was 2.28:1.The diameter of popliteal vein and femoral vein in affected side limb was respectively (8.8±1.2) mm and (12.9±2.7) mm,which was significantly larger than those in normal side limb[ (5.9 ±1.5) mm and (9.2±2.1 ) mm](P< 0.05).ConclusionHigh frequency two-dimensional and color Doppler ultrasonography has high precision in the diagnosis of lower limb deep venous thrombosis and with certain sensibility and specificity.
7.The effect of tuftsin and thymopentin on phagocytic function in mice
Qinhui XU ; Pingping YANG ; Kangtai RONG
Chinese Journal of Immunology 1985;0(02):-
The stimulate effects of synthetical endogenous peptides, tuftsin (Tuf) and thymopentin(TP5), on phagocytic function were studied. After subcutaneous administration (sc) of Tuf, thelysozyme content in both M? and sera, and the acid phosphatase activity in M?, were increasedin a dose-dependent manner. The peak time of serum lysozme level following a sigle sc injectionof Tuf was about on the 6th day. The elevation of lysozyme in sera was delayed and enhancedstrongly by Freund incomplete adjuvant (FIA). In cylcophosphamide (Cy) treated mice,TP5further increased both lysozyme content amd acid phosphatase activity in M?activated byCy, and recovered the lysozyme level in spleen inhibited by cy. The results suggested thatTP5could not only activate the funceion of M?, but recover the number of M?decreased by im-mumoimhibitant.
8.EXPERIMENT RESEARCH OF CHINESE MEDICINE (YI QI XIAO ZHENG DECOCTION)FOR RADIATION SIDE EFFECT
Pingping LI ; Buo XU ; Xinyu YANG
Chinese Journal of Radiation Oncology 1995;0(02):-
In order to evaluate the effect of chinese medicine (Yi Qi Xiao Zheng decoction) in reducing radiation side effect, an observation of peripheral blood WBC, stem cell amount, the function of liver and renal in mice was made during and after radiation.The results showed that WBC and stem cell amount were higher in Chinese medicine combined with radiation group than radiation only group after 3 fraction radiation(P
9.Effects of norepinephrine on lung injury in acute respiratory distress syndrome goats with inhaled nitric oxide
Shaohua LIU ; Xinrong XU ; Ji XU ; Xiangrong ZUO ; Pingping QIN
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To observe the effects of norepinephrine (NE) on the cardiopulmonary and lung injury in goats with acute respiratory distress syndrome (ARDS) induced by endotoxin. METHODS: A model of septic ARDS was induced by intravenous infusion of low dose endotoxin in six goats. Then 40?10~ -6 NO inhalation was applied to the animals. After 30 min, combined intravenous infusion of NE at concentration of 0.5 mg?kg~ -1 ?min~ -1 was conducted. The dynamic changes in gas exchange and hemodynamics were measured with the aid of Swan-Ganz catheter. Arterial blood gas analysis before and after the onset of ARDS, 30 min after NO inhalation and combined NE was detected. Histology of the lung was also observed. RESULTS: Inhalation of NO rapidly reduced mean pulmonary arterial pressure (MPAP), increased PaO_2, decreased P_ (A-a) O_2 and Qs/Qt in septic ARDS goats. These decrease and increase were more significant than those in NO inhaled alone when animals received NE. The combination of NO inhalation and NE injection resulted in increase in mean arterial pressure. NO inhalation did not ameliorate lung injury and combined NE intravenous injection ameliorated lung injury. CONCLUSION: Injection of low dose norepinephrine improves the beneficial effects of inhaled nitric oxide on lung gas exchange and ameliorates lung injury in goats with acute respiratory distress syndrome induced by endotoxin.
10.Study of oncogene DJ-1 expression in renal interstitial fibrosis
Honglan WEI ; Pingping LIANG ; Dixin LI ; Lin LIU ; Gang XU
Chinese Journal of Nephrology 2009;25(4):288-293
Objective To observe the expression and localization of D J-1 in renal fibrosis, and to investigate the expressions of E-cadherin, vimentin and the level of β-catenin tyrosine phosphorylation in human tubular epithelial cells. Methods In vitro, the human tubular epithehal cells (HKC cell line) were cultured with 10 μg/L TGF-β1 for 72 h. The protein expressions of E-cadherin, vimentin and DJ-1 were measured by Western blot. RT-PCR was used to detect the expression of D J-1 mRNA. The intracellular distribution of DJ-1 was observed by confocal microscope. In vivo, Masson stain was used to evaluate the level of renal fibrosis. The expression and disposition of DJ-1 in renal tissue were detected by immunohistochemistry. HKC cells were transfected with pEGFP-N1-DJ-1 via lipofectamine 2000. The efficiency of transfection was detected by fluorescence microscope. The expressions of DJ-1, E-cadherin, vimentin and β-catenin tyrosine phosphorylation level in the transfected cells were detected by Western blot. Results The expressions of DJ-1 protein and DJ-1 mRNA were up-regulated in renal tubular EMT cells. Most of DJ-1 protein localized in cytoplasm, and some was in nucleus. After stimulation by TGF-β1, the expressions of DJ-1 protein both in cytoplasm and nucleus was greatly increased, especially in nucleus. In vivo, renal tissue expressed DJ-1 in tubular epithelia, but little expression in glomeruli. In renal tissue from 5/6-nephrectomized rots, DJ-1 expression was greatly increased. In the DJ-1 transfectants, the expressions of DJ-1, vimentin and β-catenin tyrosine phosphorylation level were up-regulated, but E-cadherin expression was suppressed. Conclusion The increased expression of DJ-1 may promote renal fibrosis.