The pathogenesis of congenital heart disease can not be explained by a simple genetic mechanism.Currently,it is recognized by the interaction of genetic and environmental factors.The environmental factors change epigenetic conditions in body.Methylation is the earliest research aspect in epigenetics.However,methylation in congenital heart disease is not being given enough attention until recent years.Therefore,this review describes the relationship between methylation and congenital heart disease,in order to make us understand the pathogenesis of congenital heart disease in-depthly.

Objective Through the UVB rays radiation skin injury model ,the study investigated the effect of seabuckthorn flavonoids on the protection and restoration of the skin ,in order to provide experimental basis of looking for the new drugs against ultraviolet injury .Methods By UVB radiating repeatedly ,the study built UVB radiation skin injury model in mice ,to observed the morphological changes of the skin in every groups of experimental animal ,the super oxide dismutase (SOD) activity of the skin and the peroxidation damage degree of the skin lipid ,which is also the content changes of malondialdehyde (MDA) and collagen .Results The change of model group whose epidermis layer increased and the dermis thickened was more obvious than the normal group ,so the UVB radiation skin injury model was built successfully .The model group compared with the normal group ,its SOD activity was significantly lower and its MDA content was significantly higher (P< 0 .01) .However ,the SOD activities of positive control group and being fed with seabuckthorn flavonoids group were significantly higher and their MDA contents were significantly lower (P<0 .01) .The model group compared with the normal group ,its collagen content was significantly lower (P< 0 .05) ,and the collagen contents of positive control group and being fed with seabuckthorn flavonoids group were higher ,whose difference was extremely significant(P< 0 .01) .However ,the positive control group had hyperplastic scar .Conclusion Seabuckthorn flavonoids had the fol‐lowing effects :preventing the UVB repeating radiation damage to the skin ,improving the SOD activity of skin tissue and the colla‐gen content ,inhibiting the increase of MDA content and scar hyperplasia ,and having antagonism effect of UVB causing mice skin photoaging .

Objective:To investigate the effect and the mechanism of interleukin-18 binding protein(IL-18BP) on acute graft-versus-host disease(aGVHD) in allogeneic bone marrow transplantation(allo-BMT) in mice.Methods:C57BL/6 recipient mice irradiated with 60Co were randomly divided into 2 groups: group A and B and were transfused with BALB/c donor cells.After transplantation recipient mice in group A were treated with NS subcutaneously daily from day 0 to day 5 and recipient mice in group B with 1 ?g IL-18BP,separately.The severity of aGVHD was evaluated in the two groups.Concentrations of IFN-?,IL-2,IL-4 and IL-10 were measured using enzyme-linked immunosorbent assay(ELISA).Results:aGVHD response was more severe in group A than that in group B.The serum concentrations of IFN-? and IL-2 in group A were higher than that in group B,while the serum concentrations of IL-10 and IL-4 were lower than that in group B.Conclusion:①IL-18BP can decrese aGVHD response;②That IL-18BP can promote the differentiation of CD4~+T cells into CD4~+Th2 cells,that is promoted by IL-18BP might be one of the mechanisms of decreasing aGVHD.

Objective To observe the therapeutic effect of Swertia mussotii Franch is extracted by alcohol on immunological liver injury in mice,and further evaluate its immun-regulating effect in decreasing aminotransferase and protecting heptocytes. Methods BCG vaccine and lipopolysaccharide were intravenously injected to establish liver injury model.The effects of Swertia mussotii Franch on liver injury were contrasted. Results The high-,medium-and low-dose Swertia mussotii Franch groups which had been administered for 10 consecutive days,had significantly inhibited serum ALT and AST acticities of immunological liver injury;Swertia mussotii Franch could effectively prevent immunological liver injury by BCG+LPS. Conclusion Swertia mussotii Franch can protect mice from immunological liver injury and protect the function of liver.

In this paper, anatomy of the liver and voluminal percentage of the hepatic segments in whole liver in twenty-two men's liver were studied.The results revealed that the average volume of the liver is 1289 cm3; the mean volume is 22.3cm3 in segment I, and is 9.9%,11.6%, 16.6%,17.0%,14.6%,14.7%,15.6% insegment II to VIII respectively.

Objective: To observe the clinical efficacy of different media in pediatric tuina treatment for children with muscular torticollis. Methods: A total of 80 children with muscular torticollis were randomized into an observation group and a control group, 40 cases in each group. Both groups received conventional pediatric tuina therapy. The observation group was treated with Bai Mai ointment as the massage medium, while the common lubrication oil was used as medium in the control group. Both groups were treated five times a week, 20 min for each time, 20 times constituted a course of treatment. The traditional Chinese medicine (TCM) syndrome scores were evaluated before and after treatment, and the curative effect was observed after 3 courses and 6 courses of treatment respectively. Results: The TCM syndrome scores improved in both groups after treatment, and the intra-group differences were statistically significant (both P<0.01). The score of the observation group was statistically different from that in the control group (P<0.05). In the observation group, the total effective rates after 3 courses and 6 courses of treatment were 95.0%and 100.0%, respectively, versus 87.5% and 95.0% in the control group. The total effective rates of the two groups were statistically different (both P<0.05). Conclusion: Bai Mai ointment has a better curative effect than the common lubricating oil as a massage medium in the treatment of children with muscular torticollis.

Objective To investigate the clinical effect ofKudiezi injection combined with rosuvastatin on patients with acute cerebral infarction(ACI).Methods A total of 90 patients with ACI patients of Department of Neurology of our hospital, from March 2015 to June 2016, were randomly divided into treatment group Ⅰ, treatment group Ⅱ and the control group, 30 in each group. The control group was given atorvastatin, the treatment group Ⅰ received rosuvastatin, and the treatment group Ⅱ was adopted Kudiezi injection combined with rosuvastatin. The curative effect was observed, and blood lipid and coagulation function were compared, and daily life ability and nerve function were recorded.Results The curative ratein treatment group Ⅱ (96.7%)was significantly better than that in treatment group Ⅰ (76.7%) and the control group (73.3%) (χ2=6.537,P<0.05). Total cholesterol,three acyl glycerin,low density lipoprotein in treatment group Ⅱ (4.03 ± 0.83 mmol/L, 1.68 ± 0.46 mmol/L, 2.37 ± 0.48 mmol/Lvs. 4.61 ± 0.89 mmol/L, 2.02 ± 0.81 mmol/L, 2.74 ± 0.68 mmol/L and 5.42 ± 0.75 mmol/L, 2.38 ± 0.67 mmol/L, 3.11 ± 0.81 mmol/L) were significantly lower than those in treatment group Ⅰ and the control group (F=21.538, 8.585, 9.092,P<0.05), and those in treatment group Ⅰ was significantly lower than those in the control group (P<0.05).The fibrinogen (2.48 ± 0.37 g/Lvs.2.81 ± 0.46 g/L, 2.95 ± 0.51 g/L), platelet counts[(125.27 ± 11.88)×109vs.(132.13 ± 13.55)×109 and (133.83 ± 13.91)×109] and prothrombin time (17.52 ± 1.94 svs.(16.14 ± 1.62 s, 15.34 ± 1.18 s) in treatment group Ⅱ were significantly lower and higher than those in treatment group Ⅰ and control group (F=8.592, 3.571, 14.139,P<0.05). The scores of Barthel index (85.63 ± 4.10 vs.81.83 ± 3.92, 79.23 ± 4.81) and NIHSS (3.57 ± 1.17 vs. 4.63 ± 1.22, 5.57 ± 1.33) intreatment group Ⅱ were significantly higher and lower than those in treatment group Ⅰand the control group (F=16.873, 19.550,P<0.05), those in treatment group Ⅰ was significantly higher and lower than those in the control group (P<0.05).ConclusionsKudiezi injection combined with rosuvastatin could improve curative effect ,reduce blood lipid, improve coagulation function, improve quality of life and promote recovery of nerve function in patients with acute cerebral infarction.

Left atrial volume index (LAVI) is related to systolic and diastolic function of heart .It can reflect the se‐verity of left atrial dilation and heart failure (HF) ,and is a powerful index predicting poor prognosis of HF .This article made a review on the relationship among cardiac function ,prognosis and LAVI in HF patients ,and the diag‐nostic value of LAVI for HF patients .

Objective To discuss the efficacy and safety of sitagliptin combined with metformin in treatment of type 2 diabetes mellitus(T2DM).Methods One hundred patients with T2DM were divided into two groups by random digits table method with 50 cases each.The patients in study group were given phosphate sitagliptin (100 mg,day 1 oral) combined with metformin (500 mg,day 3 oral).The patients in control group were given repaglinide (1 mg,day 3 oral) combined with metformin (500 mg,day 3 oral).They were treated for 12 weeks and fasting plasma glucose(FPG),2 h postprandial blood glucose(2 h PG),glycosylated hemoglobin (HbA1c),fasting insulin (FINS),body mass index (BMI) and the incidence of hypoglycemic events were detected before and after treatment.Results After treatment,the level of FPG,2 h PG,HbA1c was significantly lower than that before treatment in two groups,and there was significant difference (P ＜ 0.01).The level of B MI after treatment was significantly decreased [(24.45 ± 2.13) kg/m2 vs.(25.46 ±2.62) kg/m2] and FINS was significantly increased [(13.36 ± 1.89) mU/L vs.(12.36 ± 1.80)mU/L] compared with that before treatment in study group (P ＜ 0.05 or ＜ 0.01).But the level of BMI and FINS had no significant difference before and after treatment in control group (P＞ 0.05).The incidence of hypoglycemic events had significant difference between study group and control group [0 vs.12％ (6/50)] (P ＜ 0.05).Conclusions Sitagliptin combined with metformin is safe and effective in treatment of type 2 diabetes mellitus,which is the preferred solution treatment.The risk of hypoglycaemia events is obviously reduced,also it can control and reduce weight and other advantages.

Forkhead box Q1 (FoxQ1) is a transcription factor protein with the function of cell differentiation regulation.Recently,increasing evidence has demonstrated that FoxQ1 is significantly associated with pathogenesis of digestive system tumors.This article systematically reviewed the expression and the role of FoxQ1 in the pathogenesis of digestive system tumors.Potential therapeutic target of FoxQ1 was discussed as well.